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| 1 | Experimental study on enhancement of the metastatic potential of portal vein tumor thrombus-originated hepatocellular carcinoma cells using portal vein serum显示文摘Objective: Portal vein metastasis of hepatocellular carcinoma(HCC) results in a poor prognosis and seriously affects the survival rate of patients. The mechanism underlying the formation of portal vein tumor thrombus(PVTT) is complex and is not yet fully understood. This study was conducted to investigate the impact of portal vein blood on the proliferation, metastasis, invasion and apoptosis of PVTT cells and to explore its possible mechanisms, which was expected to lay a foundation for ascertaining the mechanism underlying the portal vein metastasis of HCC.Methods: Peripheral blood and portal vein blood were collected from patients with HCC, and the sera from these two sources were used to culture the PVTT-originated HCC cell line CSQT-2. The cells were collected after 24 h, and flow cytometry was performed to detect cell proliferation, cell cycle stages and apoptosis. Transwell migration and invasion assays were applied to detect the metastasis and invasion of the cells in each group. The changes in the expression of MMP-2 and MMP-9 in cells were detected via Western blotting. The contents of IL-12, IFN-γ, IL-1β, IL-2 and TNF-α in the two groups of sera were quantified using corresponding kits. Results: Compared with the group of cells cultured with peripheral serum, the cells cultured with portal vein serum showed significantly lower apoptosis(P<0.01), significantly enhanced cell metastasis and invasion(P<0.01), whereas cell proliferation and the stages of the cell cycle did not differ significantly(P>0.05). A significantly increased expression level of MMP-2 has been observed in tumor cells treated portal vein serum. In addition, compared with peripheral serum, the content of IL-12 was significantly decreased in portal vein serum(P<0.05), while the contents of IFN-γ, IL-1β, IL-2, and TNF-α did not differ significantly(P>0.05). Conclusions: Portal vein serum from HCC patients could inhibit the apoptosis of PVTT-originated HCC cells and promote cell metastasis and invasion. This effect may be related to the lower level of IL-12 in portal vein serum. | Yufu Tang Hongming Yu Long Zhang Kang Wang Weixing Guo Jie Shi Shupeng Liu Mengchao Wu Hongyang Wang Shuqun Cheng | 2014 | Chinese Journal of Cancer Research2014，26，5： | 2 |
| 2 | Driving mechanisms of desertification process in the Horqin Sandy Land—a case study in Zhalute Banner,Inner Mongolia of China显示文摘Both natural and human factors contributing to desertification were examined to understand the driving mechanisms of the desertification process in Zhalute Banner,Inner Mongolia of China.The coefficient of variation(CV)and climate departure index(Z)were calculated to examine the fluctuations and trends of interannual variations of temperature and precipitation;TM remote sensing data was extracted to obtain the sandy land area;linear regression analysis was used to analyze climate changes and the socio-economic evolution over the years,and it was also used to standardize the variables,which included annual temperature,annual precipitation,human population,and livestock number,in order to measure the difference in the rate of change between climate and anthropogenic factors.The results showed that there was a rise of about 1.6uC in temperature but no significant change in precipitation from 1961 to 2000,which indicated a short-term climatic trend toward aridity in this area,a condition necessary for desertification.The fraction of precipitation in spring tended to increase whilst the fraction in autumn and winter decreased.Both the human population and livestock population had tripled and the cultivated area had doubled from 1961 to 2000,suggesting that socio-economic factors might have contributed more significantly to the desertification.Between 1988 and 1997,the sandy land area increased by 12.5%,nearly 2.4 times in the farming section.It could be concluded that the driving mechanisms of the desertification processes in Zhalute banner are mainly the policy of cropland expansion and the rising populations of humans and their livestock,which has affected the land use pattern in the past decades. | Haiping TANG Yufu CHEN Xinyu LI | 2008 | Frontiers of Environmental Science & Engineering2008，2，4： | 1 |
| 3 | Morphology-Tunable Fluorescent Nanoparticles: Synthesis, Photophysical Properties and Two-Photon Cell Imaging显示文摘一篇小说水溶性荧光灯 amphiphile 基于氨基的聚乙烯乙二醇(PEG-NH 2) 代替了 oligo (p-phenyleneethynylene )( 打开) 成功地被设计并且综合。拿阴离子打开是的 amphiphile 比较, photophysical 特征通过紫外吸收被调查(紫外) 并且光致发光(PL ) 分析。由于 OPE 的恐水病吸水的性质结合了分子,有从“谷物”与形态学的变化一起从 19.6 ~ 93.5 nm 到“草莓”的尺寸的自我装配的 nanoparticles 方便地经由调整集中被准备打开水的答案。在为时间的一个时期的老化以后,有趣地,同类的空 nanospheres 自发地与大约 200 nm 的一条直径被构造。Cytotoxicity 测试和 nanoparticles 的细胞的举起行为进一步被调查评估他们的潜在的生物医学的应用。随后,二光子的房间成像的有希望的应用用人的胰腺的癌症房间(PANC-1 房间) 被探索,它显示 nanoparticles 主要位于房间细胞质以内。 | Xiaoming Hu Chao Yin Wenbo Hu Zhen Yang Jie Li Xiang Li Xiaomei Lu Hui Zhao Yufu Tang Quli Fan Wei Huang | 2015 | Chinese Journal of Chemistry2015，33，8： | 0 |
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