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您的检索式：作者名="Yixiu Zhong"
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| 1 | Multitranscriptome analyses of keloid fibroblasts reveal the role of the HIF-1α/HOXC6/ERK axis in keloid development显示文摘Background:A keloid is a disease of excessive fibrosis that is characterized by the aberrant prolifer-ation of fibroblasts.However,the molecular mechanisms of fibroblasts during the development of keloids remain unclear.This study aims to identify new molecular targets that promote the proliferation and migration of keloid fibroblasts,providing new ideas for the prevention and treatment of keloids.Methods:We utilized bioinformatics tools to analyze data from keloid fibroblasts(KFs)available in the Gene Expression Omnibus(GEO)database to identify the key genes involved in keloid development.Homeobox C6(HOXC6)emerged as a hub gene in KFs from the GEO database was verified in keloid tissue samples and KFs using reverse transcription-quantitative polymerase chain reaction,western blot(WB)and immunohistochemistry.Subsequently,the effects of down-regulated HOXC6 expression on the cellular behaviors of KFs were examined by performing Cell Counting Kit-8,flow cytometry,transwell migration and WB assays.Meanwhile,we performed transcriptome sequencing and gene set enrichment analysis(GSEA)to further explore HOXC6-related mechanisms and validated the signaling pathways by performing a series of experiments.Results:HOXC6 was the top-ranking hub gene of KFs in microarray datasets from GEO and was upregulated in keloid tissue samples and KFs.Downregulation of HOXC6 inhibited proliferation,migration and extracellular matrix(ECM)accumulation and promoted KF apoptosis.GSEA pre-dicted that the hypoxia signaling pathway was associated with HOXC6 in KFs.Transcriptome sequencing suggested that the extracellular regulated protein kinase(ERK)pathway was one of the downstream pathways of HOXC6 in KFs.Our experiments confirmed that hypoxia-inducible factor-1α(HIF-1α)upregulates HOXC6,contributing to KFs proliferation,migration,apoptosis inhibition and collagen accumulation through the ERK signaling pathway.Conclusions:Our findings first revealed that HOXC6 acts as an oncogenic driver in the molec-ular mechanisms of fibroblasts in keloids.The HIF-1α/HOXC6/ERK axis promotes proliferation,migration and ECM production by KFs,contributing to the progression of keloids.Taken together,HOXC6 may serve as a promising novel therapeutic target and new focus for research designed to understand the pathogenesis of keloids. | Qi Wang Yixiu Zhong Zhijia Li Dingheng Zhu Hongyan Lu Pingjiao Chen Changxing Li Xuebiao Peng Qian Li Kang Zeng | 2022 | Burns & Trauma2022，10，1： | 0 |
| 2 | Exosomes-loaded electroconductive nerve dressing for nerve regeneration and pain relief against diabetic peripheral nerve injury显示文摘Over the years,electroconductive hydrogels(ECHs)have been extensively applied for stimulating nerve regeneration and restoring locomotor function after peripheral nerve injury(PNI)with diabetes,given their favorable mechanical and electrical properties identical to endogenous nerve tissue.Nevertheless,PNI causes the loss of locomotor function and inflammatory pain,especially in diabetic patients.It has been established that bone marrow stem cells-derived exosomes(BMSCs-Exos)have analgesic,anti-inflammatory and tissue regeneration properties.Herein,we designed an ECH loaded with BMSCs-Exos(ECH-Exos)electroconductive nerve dressing to treat diabetic PNI to achieve functional recovery and pain relief.Given its potent adhesive and self-healing properties,this laminar dressing is convenient for the treatment of damaged nerve fibers by automatically wrapping around them to form a size-matched tube-like structure,avoiding the cumbersome implantation process.Our in vitro studies showed that ECH-Exos could facilitate the attachment and migration of Schwann cells.Meanwhile,Exos in this system could modulate M2 macrophage polarization via the NF-κB pathway,thereby attenuating inflammatory pain in diabetic PNI.Additionally,ECH-Exos enhanced myelinated axonal regeneration via the MEK/ERK pathway in vitro and in vivo,consequently ameliorating muscle denervation atrophy and further promoting functional restoration.Our findings suggest that the ECH-Exos system has huge prospects for nerve regeneration,functional restoration and pain relief in patients with diabetic PNI. | Qinfeng Yang Shenghui Su Shencai Liu Sheng Yang Jing Xu Yixiu Zhong Yusheng Yang Liangjie Tian Zilin Tan Jian Wang Zhiqiang Yu Zhanjun Shi Fangguo Liang | 2023 | Bioactive Materials2023，，8： | 0 |
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