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| 1 | Hes1 Knockdown Exacerbates Ischemic Stroke Following tMCAO by Increasing ER Stress-Dependent Apoptosis via the PERK/ eIF2a/ATF4/CHOP Signaling Pathway显示文摘Apoptosis induced by endoplasmic reticulum(ER)stress plays a crucial role in mediating brain damage after ischemic stroke.Recently,Hes1(hairy and enhancer of split 1)has been implicated in the regulation of ER stress,but whether it plays a functional role after ischemic stroke and the underlying mechanism remain unclear.In this study,using a mouse model of ischemic stroke via transient middle cerebral artery occlusion(tMCAO),we found that Hes1 was induced following brain injury,and that siRNA-mediated knockdown of Hes1 increased the cerebral infarction and worsened the neurological outcome,suggesting that Hes1 knockdown exacerbates ischemic stroke.In addition,mechanistically,Hes1 knockdown promoted apoptosis and activated the PERK/eIF2a/ATF4/CHOP signaling pathway after tMCAO.These results suggest that Hes1 knockdown promotes ER stress-induced apoptosis.Furthermore,inhibition of PERK with the specific inhibitor GSK2606414 markedly attenuated the Hes1 knockdown-induced apoptosis and the increased cerebral infarction as well as the worsened neurological outcome following tMCAO,implying that the protection of Hes1 against ischemic stroke is associated with the amelioration of ER stress via modulating the PERK/eIF2a/ATF4/CHOP signaling pathway.Taken together,these results unveil the detrimental role of Hes1 knockdown after ischemic stroke and further relate it to the regulation of ER stress-induced apoptosis,thus highlighting the importance of targeting ER stress in the treatment of ischemic stroke. | Yueyong Li Yingjun Zhang Huangde Fu Huadong Huang Qifeng Lu Houji Qin Yingning Wu Huatuo Huang Guizhen Mao Zhongheng Wei Pinhu Liao | 2020 | Neuroscience Bulletin2020，36，2： | 12 |
| 2 | On detection performance and system configuration of MIMO radar显示文摘Multiple-input multiple-output (MIMO) radar is a new concept with some new characteristics,such as multiple orthogonal waveforms and omnidirectional coverage. Based on Stein's lemma,we use relative entropy as a precise and general measure of error exponent to study detection performance for both MIMO radar and phased array radar. And based on derived analytical results,we further study the system configuration problem of Bistatic MIMO radar systems,where transmitters and receivers are located in different positions. Some interesting results are presented. For phased array radar,when the total numbers of transmitters and receivers are fixed,we should always make the number of transmitters equal to the number of receivers. For MIMO radar,we should use a small number of transmitters in low signal noise ratio (SNR) region,and make the number of transmitters equal to the number of receivers in high SNR region. These results are instructive for deployment of bistatic MIMO radar systems in the future. | TANG Jun WU Yong PENG YingNing WANG XiuTan | 2009 | Science in China(Series F)2009，52，7： | 7 |
| 3 | Detection and Analysis of Routine Blood Indexes in Guike Pig显示文摘[Objectives] In this study,routine blood indexes of Guike pig were detected to clarify quantity variation and morphology distribution of different functional cells( red blood cells,white blood cells and platelets),thus providing reference data for immunological evaluation and disease resistance research of Guike pig. [Methods] Blood samples were collected from forty-four 49-day-old healthy Guike pigs( 22 males and 22 females) once a week for seven consecutive weeks to detect routine blood indexes,and the results were compared with routine blood indexes of Landrace pig and Enshi black pig. [Results]Among red blood cell indexes of Guike pig at different day ages,red blood cell count( RBC) and hemoglobin count( HGB) both reached the lowest levels( 6. 85 × 1012/L and 97. 1 g/L)at 70 days of age,which were significantly lower than the corresponding indexes at 49,56,63,84 and 91 days of age( P < 0. 05,similarly hereinafter); mean corpuscular hemoglobin( MCH) and mean corpuscular hemoglobin concentration( MCHC) demonstrated basically the same variation pattern,which both reached the lowest levels( 15. 11 pg and 275. 70 g/L) at 70 days of age and reached the highest levels( 16. 40 pg and 314. 30 g/L) at 91 days of age; hematocrit( HCT),mean corpuscular volume( MCV) and red blood cell distribution width( RDW) exhibited relatively stable changes with no significant differences among different day ages( P > 0. 05,similarly hereinafter). Among white blood cell indexes of Guike pig at different day ages,white blood cell count( WBC) reached the lowest level( 13. 71 × 109/L) at 63 days of age and reached the highest level( 17. 52 × 109/L) at 84 days of age,with significant differences; neutrophil count( NEUT)reached the lowest level( 3. 61 × 109/L) at 70 days of age,which was significantly lower than that at 49,56 and 77 days of age; lymphocyte count( LYMPH) exhibited relatively stable changes and was not significantly affected by the increase in day age. Among platelet indexes of Guike pig at different day ages,platelet count( PLT),thrombocytocrit( PCT) and platelet volume distribution width( PDW) exhibited relatively stable changes among different day ages,which were not significantly affected by the increase in day age. Compared to 35-day-old Landrace pig and 5-month-old Enshi black pig,most routine blood indexes of Guike pig were similar to or ranged between those of Landrace pig and Enshi black pig except HCT,mean platelet volume( MPV),PCT and PDW. [Conclusions]Guike pig has a certain uniqueness in routine blood indexes,which has developed a stable immune system at 49-91 days of age. | Qianlian SU Bin LI Jiaxing LIANG Yingning ZHOU Zhongwei CHEN Yibin QIN Bingxia LU Ying HE Qunpeng DUAN Dongfu JIANG Jingzhuan LU Wu ZHAO | 2018 | Agricultural Biotechnology2018，7，3： | 6 |
| 4 | Detection of Porcine Epidemic Diarrhea Virus in Guangxi Province from 2011 to 2014 and Sequence Analysis of Its M Gene显示文摘Detection of pigs epidemic diarrhea virus( PEDV) was conducted on 331 piglets diarrhea fecal samples collected in Nanning,Yulin and other 12 areas of Guangxi Province from January of 2011 to April of 2014 by the method of reverse transcription-polymerase chain reaction( RT-PCR). The results showed that the positive samples of PEDV were 210 and the positive rate was 63. 44%. The clone and sequencing of M gene was carried out on 25 positive samples. PEDV reference strains were selected from Gene Bank to conduct the sequence homology alignment analysis and the phylogenetic tree of M gene. The M gene homology and amino acid sequence identity between 25 isolated strains and 51 reference strains were 96. 0%- 99. 6% and 94. 3%- 99. 6%,respectively. The genetic variation analysis of M gene showed that the genetic relationship of PEDV prevalent strains in Guangxi Province from 2013 to 2014 was close to that of the prevalent strains in Beijing,Anhui,Wuhan,Hebei and Guangdong from 2010 to 2013,and which were far from that of the Chinese early isolates CH / S( Gen Bank number: JN547228),vaccine strain CV777( Gen Bank number: AF353511) and Attenuated DR13( Gen Bank number: JQ023162). Indicating that the PEDV strains prevalent in Guangxi in recent years showed significant variation with the early isolates. | Lu Bingxia Qin Yibin He Ying Li Yingying Liang Jiaxing Li Keyu Li Bin Su Qianlian Zhou Yingning Jiang Dongfu Lu Jingzhuan Bi Bingfen Liang Baozhong Duan Qunpeng Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，1： | 3 |
| 5 | On detection performance of MIMO radar for Rician target显示文摘By using spatial diversity, multiple-input-multiple-output (MIMO) radar can improve detection performance for fluctuating targets. In this paper, we propose a spatial fluctuation target model for MIMO radar, where targets are classified as non-fluctuating target, Rayleigh target and Rician target. Based on Stein’s lemma, we use relative entropy to study detection performance of optimum detector for Rician target. It is found that in low signal noise ratio (SNR) region, the performance improvement of MIMO radar for detecting Rician target depends on array gain, which is related to the number of receivers. In high SNR region, the improvement of performance depends on diversity gain, which is related to the product of the number of receivers and the number of transmitters. The conclusions of this paper are important for designing MIMO radar system. | TANG Jun WU Yong PENG YingNing WANG XiuTan | 2009 | Science in China(Series F)2009，52，8： | 2 |
| 6 | Models and performance evaluation for multiple-input multiple- output space-time adaptive processing radar显示文摘 | Wu Yong Tang Jun Peng Yingning | 2009 | IET Radar Sonar I: Navigation2009，3，6： | 1 |
| 7 | Improved soil structure and citrus growth after inoculation with three arbuscular mycorrhizal fungi under drought stress 显示文摘 | WU Qiangsheng XIA Renxue ZOU Yingning | 2008 | European Journal of Soil Biology2008，44，： | 1 |
| 8 | Contributions of arbuscular mycorrhizal fungi to growth, photosynthesis, root morphology and ionic balance of citrus seedlings under salt stress 显示文摘 | Wu Qiangsheng Zou Yingning He Xinhua | 2010 | Acta Physiol Plant2010，，32： | 1 |
| 9 | Improved soil structure and citrus growth after inoculation with three arbuscularmycorrhizal fungi under drought stress显示文摘 | Wu Qiangheng Xia Renxue Zou Yingning | 2008 | European journal of soil biology2008，44，： | 1 |
| 10 | Spatial distribution of glomalin-related soil protein and its relation- ships with root mycorrhization, soil aaggregates, carbohy- drates,activity of protease and β-glucosidase in the rhizo- sphere of Citrus unshlu显示文摘 | Wu Qiangsheng He Xinhua Zou Yingning | | Soil Biology and Biochemistry0，45，： | 1 |
| 11 | On the essence of knowledge-aided clutter covariance estimate and its convergence显示文摘 | Yong Wu Jun Tang Yingning Peng | 2011 | IEEE Transactions on Aerospace and Electronic Systems2011，47，1： | 1 |
| 12 | Isolation of Swine Corynebacteria and Analysis of 16S rDNA Gene显示文摘A strain of gram-positive bacillus was isolated from suppurative lung organs of nursery pigs in a pig farm,which was further characterized by morphological observation,cultivation test,biochemical test,drug sensitivity test,pathogenicity test and 16 S rDNA gene cloning and sequence analysis. The results showed that the isolate grew well in rabbit blood agar plate and horse serum tryptone soybean agar( TSA) plate under aerobic condition,which could lead to mortality of mice and were susceptible to cephalosporin antibiotics and fluoroquinolone antibiotics. Phylogenetic analysis showed that the isolate had close genetic evolutionary relationships with Corynebacterium bacteria,and the sequence of 16 S rRNA gene shared the homology of 91. 7%-98. 3% with the representative strain of corynebacteria,indicating the isolated strain was corynebacterium. | Qin Yibin He Pingping Lu Bingxia He Ying Li Bin Liang Jiaxing Duan Qunpeng Chen Zhongwei Bi Bingfen Zhou Yingning Su Qianlian Jiang Dongfu Li Keyu Lu Jingzhuan Zhao Wu | 2016 | Animal Husbandry and Feed Science2016，8，6： | 0 |
| 13 | Phylogenetic Analysis of HN Gene of Eight Pigeon NDV Isolates in Guangxi显示文摘[Objective] The paper was to provide a basis for scientific prevention and control of pigeon Newcastle disease(ND).[Method] The HN gene of eight pigeon NDV strains isolated from different pigeon farms in Guangxi were amplified by RT-PCR,sequenced and analyzed.The molecular evolution characteristics of HN gene of pigeon NDV isolates in Guangxi was discussed.[Result] The nucleotide sequence length of HN gene of the eight NDV isolates was 1 716 bp,encoding 571 amino acids.They belonged to virulent group C,and the gene length characteristic of HN gene accorded with virulent strain.Analysis of nucleotide homologies indicated that the eight NDV isolates shared higher homology with genotype VIb,ranging from 90.4% to 99.5%.Phylogenetic tree analysis demonstrated that the genetic relationship between the eight NDV strains in Gangxi and the NDV isolates from Guangxi,Guangdong,Jilin,Liaoning,Yunnan and Heilongjiang during 2011 and 2013 was close.They were located in the same cladogram branch.[Conclusion] We assume that the eight pigeon NDV isolates in Guangxi all belong to the gene class II genotype VI b NDV. | Lu Bingxia Liang Jiaxing Duan Qunpeng Chen Zhongwei Jiang Dongfu Lu Jingzhuan Zhou Yingning Bi Bingfen He Ying Qin Yibin Li Bin Su Qianlian Zhao Wu | 2018 | Animal Husbandry and Feed Science2018，10，2： | 0 |
| 14 | Application of rare-earth and nano elements on diamond cup wheels显示文摘Diamond cup wheel is used widely as an important tool for machining ceramic tile.In this paper,nano rare-earth oxide and nano carbide were added in the segments of seven kinds of diamond cup wheels.The performance of diamond cup wheels were tested on a special designed test machine by grinding two kinds of ceramic tiles.The surface morphology of the segments was examined by Scanning Election Microscopy(SEM) and the micro-hardness of segments was measured.The results showed that nano rare-earth oxide and nano carbide can fine segment micro structure,make grain boundary clear and increase grasping of diamond grits.They can increase also the wear resistance of diamond cup wheels as well as the grinding ratio. | Cheng Yunping Wang Chengyong Hu Yingning Wu Wenge | 2007 | 金刚石与磨料磨具工程2007，27，5： | 0 |
| 15 | Root-associated endophytic fungi modulate endogenous auxin and cytokinin levels to improve plant biomass and root morphology of trifoliate orange显示文摘Root-associated endophytic fungi like Serendipita indica and arbuscular mycorrhizal(AM) fungi can improve plant growth and root construction, but the potential mechanism is unclear. In this study, Funneliformis mosseae(an AM fungus) and S. indica, singly or in combination were inoculated into trifoliate orange(Poncirus trifoliata) seedlings, to assess changes in biomass and root morphological traits, coupled with auxins and cytokinins concentrations in leaves and roots and the expression of auxin synthesis and transporter protein genes. After 20 weeks of inoculation with these fungi, shoot and root biomass, root total length, taproot length, average diameter, surface area, volume, and the number of lateral roots in 1st-, 2nd-, and 3rd-order were improved, and S. indica showed a relatively greater effect than F. mosseae and dual inoculation. Endophytic fungal inoculation also significantly increased the concentration of indoleacetic acid, indole butyric acid, trans-zeatin,dihydrozeatin, and isopentenyl adenine in leaves and roots, whilst F. mosseae and S. indica exhibited relatively greater effects on leaves and roots, respectively. Correlation analysis revealed that both biomass and root morphological traits(except root projected area) were significantly positively associated with endogenous auxins and cytokinins. In addition, the inoculated plants recorded comparatively higher expression levels of indoleacetic acid synthesis genes(PtTAA1, PtTAR2, PtYUC3, PtYUC4, PtYUC6, and PtYUC8) and indoleacetic acid transporter protein genes(PtAUX1, PtLAX1, PtLAX2, PtLAX3, PtPIN1, PtPIN3, PtPIN4, PtABCB1, and PtABCB19) than the non-inoculated plants, among which F. mosseae and S. indica showed better effects in leaves and roots, respectively. These results suggest that root-associated endophytic fungi improved plant growth and root architecture, which were associated with changes in endogenous auxins and cytokinins. | Ruicheng Liu Liu Yang Yingning Zou Qiangsheng Wu | 2023 | Horticultural Plant Journal2023，9，3： | 0 |
| 16 | Development and Application of an RT-PCR Method for Differentiation of PEDV Variant Strains and Classical Strains显示文摘In order to establish a rapid RT-PCR assay for detection of porcine epidemic diarrhea virus(PEDV),a pair of special primers was designed based on S gene sequence of PEDV published in Gen Bank.After optimization of the reaction system,a rapid RT-PCR method was established.Results showed that a fragment of 826 bp was successfully amplified only from the variant PEDV by RT-PCR,while the expected target fragment could not be amplified from TGEV,porcine rotavirus,porcine kobuvirus,PRRSV,PRV,CSFV,PPV.Sensitivity test of RT-PCR indicated that only 11.3 pg nuclear acids could be detected accurately and rapidly.A total of 123 samples collected from different farms in Guangxi Province were detected by the established RT-PCR,the positive rate of PEDV was 67.5%,and the positive rate of new PEDV was 86.7%(72/83).Therefore,the RT-PCR could be used as an effective tool for differentiating diagnosis of the highly pathogenic PEDV in epidemiological investigations. | Qin Yibin Lu Bingxia Zhao Wu He Ying Li Yingying Li Bin Liang Jiaxing Liang Baozhong Su Qianlian Jiang Dongfu Zhou Yingning | 2015 | Animal Husbandry and Feed Science2015，7，1： | 0 |
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