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| 1 | 百日咳毒素S1亚单位的分子修饰及在重组杆状病毒中的表达显示文摘目的:百日咳毒素既是百日咳杆菌的主要毒性因子,又是重要的保护性抗原,其S1亚单位具有ADP核糖转移酶活性和免疫保护性决定簇。为高效表达前构建的一个丧失酶活性但仍保持保护决定簇的S1变异子,开展了本研究。方法:通过添加人流感病毒血凝素基因信号序列或嵌膜序列的方法,对天然和变异S1亚单位基因片段作了遗传学修饰。然后使用重组杆状病毒技术使这些S1亚单位在昆虫细胞和昆虫幼虫中实现了高水平表达。结果:这些重组S1的表达水平介于每万个昆虫卵细胞018~193μg或每只昆虫幼虫046~498mg之间。天然信号肽和HA信号肽均可完整表达,但后者的切割效率(61%~81%)比前者(16%~19%)更高。所有带信号肽的S1亚单位均呈异质性表达(双带)。结论:重组S1亚单位的表达是高效和正确的,表达的异质性是由于信号肽切割不完全的缘故。 | 于康震 Burnette WN Kaslow HR Mar VL Ahmad S Yilma TD | 1999 | 中国免疫学杂志1999，15，2： | 4 |
| 2 | 分子修饰的重组百日咳毒素S1亚单位的生物学和免疫学特性显示文摘目的:对重组杆状病毒表达的经多种信号序列修饰的天然和变异百日咳毒素(PT)S1亚单位(rS1)的生物学及免疫学特性作出评价。方法:应用生物化学和免疫学技术与方法对这些rS1的酶活性、分泌性和免疫原性等做了系统鉴定。结果:rS1变异子无可检出的酶活性;所有rS1均不能由细胞分泌,可从细胞膜组分中大量检出,但不能从可溶性胞浆蛋白组分或细胞培养上清中检出;rS1分子内部含有3个疏水性区域;rS1虽不能与B寡聚体结合形成PT全毒素,但均能诱导抗PT免疫应答。结论:这些rS1极可能以膜蛋白形式存在,其非分泌性与分子内部的疏水性区域有关;所有rS1均保持其免疫原性。 | 于康震 Burnette W N Kaslow H R Yilma T D | 1999 | 中国免疫学杂志1999，15，4： | 4 |
| 3 | Flavonoid naringenin : a potential immunomodulator for Chlamydia trachomatis inflam- mation 显示文摘 | Yilma AN Singh SR Morici L | 2013 | Mediators Inflamm2013，5，10： | 1 |
| 4 | Satellite Climatology and the Environmental Risk of Schistosomiasis mansoni in Ethiopia and East Africa显示文摘 | Malone JB Yilma JM Mc Carroll JC | 2001 | Acta Trop2001，79，： | 1 |
| 5 | The agricultural technology market linkage under liberalization in Ghana:Evidence from micro data,Journal of African Economies显示文摘 | Yilma T Berg E Berger T | 2008 | 17(1):62-842008，17，1： | 1 |
| 6 | Perivasculas epithelioid celltumor(PEComa)of the uterine cervix associated with intraab-dominal“PEComatosis”:a clinicopathologieal study with compar-ative genomic hybridization analysis显示文摘 | Eadare O Paskash V Yilmas Y | 2004 | World J Surg Oneol2004，2，1： | 1 |
| 7 | Diagnostic value of homocys- teine, C-reactive protein and bilirubln for coronary artery disease 显示文摘 | Yilmas N Cieek HK Celik A | 2007 | East Mediterr Health2007，13，3： | 1 |
| 8 | Antimi- crohial lipids: novel innate defense molecules are ele- vated in sinus secretions of patients with chronic rhi- nosinusitis显示文摘 | LEE J T JANSEN M YILMA A N | 2010 | Am J Rhinol Allergy2010，24，： | 1 |
| 9 | A geographic information system forecast model for strategic control of fasciolosis in Ethiopia显示文摘 | Yilma J M Malone J B | 1998 | Vet Parasitol1998，74，7： | 1 |
| 10 | Transition to Ecological Urban Agriculture; a Challenge显示文摘 | Moniek van Hirtum Eric Goewie Yilma Getachew & Pene van Veenhuizen | 2002 | Urban Agriculture Magazine2002，，6： | 1 |
| 11 | The anti-inflammatory cytokine,interleukin-10,inhibits inflammatory mediators in human epithelial cells and mouse macrophages exposed to live and UV-inactivated Chlamydia trachomatis显示文摘 | Yilma AN Singh SR Fairley SJ | | 0，，： | 1 |
| 12 | Influence of some set accelerating admixtures on the hydration of Portland cement con - taining phosphogypsum 显示文摘 | YILMAS V T LSILDAK O | 1993 | Advances in Cement Research1993，5，2： | 1 |
| 13 | Satellite climatology and the environmental risk of schistosoma mansoni in Ethiopia and east African显示文摘 | Malone JB Yilma JM Mccarroll JC | 2001 | Acta Trop2001，79，1： | 1 |
| 14 | Enhancement of primary interferon and secondary immune response by interferon-gamma显示文摘 | Yilma T Owens S Fennie E H | 1998 | Adv Exp Med Biol1998，251，： | 1 |
| 15 | Accidental human vaccination with vaccinia virus expressing nucleoprotein gene显示文摘 | Jones L Ristow S Yilma T | 1986 | Nature1986，319，： | 1 |
| 16 | The anti - inflammatory cytokine, interleakin - 10, inhibits inflammatory mediators in human epithelial cells and mouse macrophages exposed to live and UV- inactivated Chlamydia trachomatis 显示文摘 | Yilma AN Singh SR Fairley SJ | 2012 | Mediators Inflamm Epub2012，，52： | 1 |
| 17 | Occurrence and distribution of species of Enterobacteriaceae in selected Ethiopian traditional dairy products:a contribution to epidemiology显示文摘 | Yilma Z Faye B Loiseau G | 2007 | Food Control2007，18，11： | 1 |
| 18 | Structure and function of long lived olfactory organotypic culture from postnatal mice显示文摘 | Josephson EM Yilma S Vodyanoy V | 2004 | J - Neurosci- Res2004，75，1： | 1 |
| 19 | Immunohistochemical analysis of biliary tract lesions显示文摘 | Tan G Yilmas A De Young BR | 2004 | Appl Immunohistochem Mol Morphol2004，12，3： | 1 |
| 20 | Sources and distribution of trace metals in the Saricay Stream basin of southwestem Turkey 显示文摘 | LEVENT T A YILMA Z F DEMIRAK A | 2007 | Environmental Monitoring and Assessment2007，125，1： | 1 |
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