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| 1 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 2 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 3 | Comparison of 5-hydroxytryptophan signaling pathway characteristics in diarrhea-predominant irritable bowel syndrome and ulcerative colitis显示文摘AIM: To study differences in the visceral sensitivity of the colonic mucosa between patients with diarrheapredominant irritable bowel syndrome(IBS-D) and those with ulcerative colitis(UC) in remission and to relate these differences with changes in the 5-hydroxytryptophan(5-HT) signaling pathway. METHODS: Gastrointestinal symptoms were used to determine the clinical symptom scores and rectal visceral sensitivity of patients with IBS-D and patients with UC in remission. Blood levels of 5-HT and5-hydroxyindoleacetic acid(5-HIAA) were measured using an HPLC-electrochemical detection system. The levels of 5-HT 3 receptor(3R), 4R, and 7R m RNAs in colonic biopsy samples were detected using reverse transcription-polymerase chain reaction. The protein expression of TPH1 was analyzed by Western blot and immunohistochemistry.RESULTS: Abdominal pain or discomfort, stool frequency, and the scores of these symptoms in combination with gastrointestinal symptoms were higher in the IBS-D and UC groups than in the control groups. However, no significant differences were observed between the IBS-D and UC remission groups. With respect to rectal visceral sensitivity, the UC remission and IBS-D groups showed a decrease in the initial perception threshold, defecating threshold and pain threshold. However, these groups exhibited significantly increased anorectal relaxation pressure. Tests examining the main indicators of the 5-HT signaling pathway showed that the plasma 5-HT levels, 5-HIAA concentrations, TPH1 expression in the colonic mucosa, and 5-HT3 R and 5-HT5 R expression were increased in both the IBS-D and the UC remission groups; no increases were observed with respect to 5-HT7 R expression.CONCLUSION: The IBS-D and UC groups showed similar clinical symptom scores, visceral sensitivity, and levels of serotonin signaling pathway indicators in the plasma and colonic mucosa. However, the pain threshold and 5-HT7 R expression in the colonic mucosa were significantly different between these groups. The results reveal that(1) IBS-D and UC are related to visceral sensitivity pathogenesis and the clinical manifestations of these conditions and(2) the observed differences in visceral hypersensitivity are possibly due to differences in levels of the 5-HT7 receptor, a component of the 5-HT signaling pathway. | Feng-Yan Yu Shao-Gang Huang Hai-Yan Zhang Hua Ye Hong-Gang Chi Ying Zou Ru-Xi Lv Xue-Bao Zheng | 2016 | World Journal of Gastroenterology2016，22，12： | 19 |
| 4 | 粉防己碱抑制前列腺癌细胞迁移和侵袭并通过诱导凋亡抑制细胞增殖显示文摘粉防己碱作为一种传统的中药,在多种肿瘤细胞中呈现显著的抗肿瘤活性。然而,粉防己碱在前列腺癌细胞中的作用却知之甚少,且其作用于前列腺癌的具体机制也尚未有人阐述。为了探究粉防己碱对前列腺癌DU145和PC-3两种细胞系生长抑制、凋亡诱导、迁移和侵袭抑制等方面的作用,通过MTT实验和克隆形成实验检测其对前列腺癌细胞的生长抑制能力,采用流式细胞仪检测其对前列腺癌细胞的凋亡诱导作用。后通过Western blotting实验检测粉防己碱处理后两种前列腺癌细胞系中PARP、Caspase-3、Akt、p-Akt、Bcl-2、Bax等蛋白的表达情况。而细胞划痕实验和transwell侵袭实验则分别用来检测粉防己碱对前列腺癌细胞的迁移和侵袭能力。结果显示,粉防己碱可剂量依赖性和时间依赖性地抑制前列腺癌细胞系DU145和PC-3的生长;且经粉防己碱处理后,两种前列腺癌细胞系的细胞克隆数明显受到抑制。且粉防己碱可抑制两种前列腺癌细胞系的侵袭,并显著抑制其迁移能力。由此可知,粉防己碱对前列腺癌细胞的增殖、迁移和侵袭有显著抑制作用。另外,粉防己碱可剂量依赖性地诱导前列腺癌细胞的凋亡,且此过程是通过caspase级联反应的激活和PI3K-Akt信号通路的抑制而得以实现的。上述结果提示粉防己碱在临床中可作为前列腺癌治疗的一种潜在治疗药物。 | Wei Liu Bo KOU Zhen-Kun Ma Xiao-Shuang Tang Chuan Lv Min Ye Jia-Qi Chen Lei Li Xin-Yang Wang Da-Lin He | 2015 | Asian Journal of Andrology2015，17，5： | 18 |
| 5 | Effects of baicalin in CD4 + CD29 + T cell subsets of ulcerative colitis patients显示文摘AIM:To evaluate the role of baicalin in ulcerative colitis(UC) with regard to the CD4+CD29+ T helper cell,its surface markers and serum inflammatory cytokines.METHODS:Flow cytometry was used to detect the percentage of CD4+CD29+ cells in patients with UC.Real time polymerase chain reaction was used to detect expression of GATA-3,forkhead box P3,T-box expressed in T cells(T-bet),and retinoic acid-related orphan nuclear hormone receptor C(RORC).Western blotting was used to analyze expression of nuclear factor-κB(NF-κB) p65,phosphorylation of NF-κB(p-NF-κB) p65,STAT4,p-STAT4,STAT6 and p-STAT6.The concentrations of interferon-γ(IFN-γ),interleukin(IL)-4,IL-5,IL-6,IL-10 and TGF-β in serum were determined by ELISA assay.RESULTS:The percentages of CD4+CD29+ T cells were lower in treatment with 40 and 20 μmol/L baicalin than in the treatment of no baicalin.Treatment with 40 or 20 μmol/L baicalin significantly upregulated expression of IL-4,TGF-β1 and IL-10,increased p-STAT6/STAT6 ratio,but downregulated expression of IFN-γ,IL-5,IL-6,RORC,Foxp3 and T-bet,and decreased ratios of T-bet/GATA-3,p-STAT4/STAT4 and p-NF-κB/NF-κB compared to the treatment of no baicalin.CONCLUSION:The results indicate that baicalin regulates immune balance and relieves the ulcerative colitis-induced inflammation reaction by promoting proliferation of CD4+CD29+ cells and modulating immunosuppressive pathways. | Feng-Yan Yu Shao-Gang Huang Hai-Yan Zhang Hua Ye Hong-Gang Chi Ying Zou Ru-Xi Lv Xue-Bao Zheng | 2014 | World Journal of Gastroenterology2014，20，41： | 18 |
| 6 | Consensus on the application of negative pressure wound therapy of diabetic foot wounds显示文摘Because China is becoming an aging society,the incidence of diabetes and diabetic foot have been increasing.Diabetic foot has become one of the main health-related killers due to its high disability and mortality rates.Negative pressure wound therapy(NPWT)is one of the most effective techniques for the treatment of diabetic foot wounds and great progress,both in terms of research and its clinical application,has been made in the last 20 years of its development.However,due to the complex pathogenesis and management of diabetic foot,irregular application of NPWT often leads to complications,such as infection,bleeding and necrosis,that seriously affect its treatment outcomes.In 2020,under the leadership of Burns,Trauma and Tissue Repair Committee of the Cross-Straits Medicine Exchange Association,the writing group for‘Consensus on the application of negative pressure wound therapy of diabetic foot wounds’was established with the participation of scholars from the specialized areas of burns,endocrinology,vascular surgery,orthopedics and wound repair.Drawing on evidence-based practice suggested by the latest clinical research,this consensus proposes the best clinical practice guidelines for the application and prognostic evaluation of NPWT for diabetic foot.The consensus aims to support the formation of standardized treatment schemes that clinicians can refer to when treating cases of diabetic foot. | Shizhao Ji Xiaobin Liu Jie Huang Junmin Bao Zhaohong Chen Chunmao Han Daifeng Hao Jingsong Hong Dahai Hu Yufeng Jiang Shang Ju Hongye Li Zongyu Li Guangping Liang Yan Liu Gaoxing Luo Guozhong Lv Xingwu Ran Zhongmin Shi Juyu Tang Aiping Wang Guangyi Wang Jiangning Wang Xin Wang Bing Wen Jun Wu Hailin Xu Maojin Xu Xiaofei Ye Liangxi Yuan Yi Zhang Shichu Xiao Zhaofan Xia | 2021 | Burns & Trauma2021，9，1： | 17 |
| 7 | Formation and control of disinfection byproducts and toxicity during reclaimed water chlorination: A review显示文摘Chlorination is essential to the safety of reclaimed water; however, this process leads to concern regarding the formation of disinfection byproducts(DBPs) and toxicity. This study reviewed the formation and control strategies for DBPs and toxicity in reclaimed water during chlorination.Both regulated and emerging DBPs have been frequently detected in reclaimed water during chlorination at a higher level than those in drinking water, indicating they pose a greater risk to humans. Luminescent bacteria and Daphnia magna acute toxicity, anti-estrogenic activity and cytotoxicity generally increased after chlorination because of the formation of DBPs. Genotoxicity by umu-test and estrogenic activity were decreased after chlorination because of destruction of toxic chemicals. During chlorination, water quality significantly impacted changes in toxicity.Ammonium tended to attenuate toxicity changes by reacting with chlorine to form chloramine,while bromide tended to aggravate toxicity changes by forming hypobromous acid. During pretreatment by ozonation and coagulation, disinfection byproduct formation potential(DBPFP)and toxicity formation potential(TFP) occasionally increase, which is accompanied by DOC removal; thus, the decrease of DOC was limited to indicate the decrease of DBPFP and TFP. It is more important to eliminate the key fraction of precursors such as hydrophobic acid and hydrophilic neutrals. During chlorination, toxicities can increase with the increasing chlorine dose and contact time. To control the excessive toxicity formation, a relatively low chlorine dose and short contact time were required. Quenching chlorine residual with reductive reagents also effectively abated the formation of toxic compounds. | Ye Du Xiao-Tong Lv Qian-Yuan Wu Da-Yin Zhang Yu-Ting Zhou Lu Peng Hong-Ying Hu | 2017 | Journal of Environmental Sciences2017，29，8： | 16 |
| 8 | Chromosomal level assembly and population sequencing of the Chinese tree shrew genome显示文摘Chinese tree shrews (Tupaia belangeri chinensis) have become an increasingly important experimental animal in biomedical research due to their close relationship to primates. An accurately sequenced and assembled genome is essential for understanding the genetic features and biology of this animal. In this study, we used long-read single-molecule sequencing and high-throughput chromosome conformation capture (Hi-C) technology to obtain a high-qualitychromosome-scale scaffolding of the Chinese tree shrew genome. The new reference genome (KIZ version 2: TS_2.0) resolved problems in presently available tree shrew genomes and enabled accurate identification of large and complex repeat regions, gene structures, and species-specific genomic structural variants. In addition, by sequencing the genomes of six Chinese tree shrew individuals, we produced a comprehensive map of 12.8 M single nucleotide polymorphisms and confirmed that the major histocompatibility complex (MHC) loci and immunoglobulin gene family exhibited high nucleotide diversity in the tree shrew genome. We updated the tree shrew genome database (TreeshrewDB v2.0: http://gffzzb70c77447da74c53hwn096punw99o6uqc.ffgz.tsg.suse.edu.cn) to include the genome annotation information and genetic variations. The new high-quality reference genome of the Chinese tree shrew and the updated TreeshrewDB will facilitate the use of this animal in many different fields of research. | Yu Fan Mao-Sen Ye Jin-Yan Zhang Ling Xu Dan-Dan Yu Tian-Le Gu Yu-Lin Yao Jia-Qi Chen Long-Bao Lv Ping Zheng Dong-Dong Wu Guo-Jie Zhang Yong-Gang Yao | 2019 | Zoological Research2019，40，6： | 15 |
| 9 | Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation显示文摘用 Yamanaka 因素产生的导致的 pluripotent 茎(iPS ) 细胞在自体同源的细胞治疗为使用有大潜力。然而, genomic 畸形充分在人的 iPS 房间,和房间不是的大多数老鼠 iPS 存在 pluripotent 由 tetraploid 互补试金评估了 TCA ) ;这很可能与 DNA 损坏反应(DDR ) 被联系在 Yamanaka 因素导致的早 reprogramming 发生了。相反,原子转移忠实地装改编体的房间进胚胎的茎(ES ) 满足 TCA 的房间。我们因此假设了涉及导致卵母细胞的 reprogramming 的因素可以在 reprogramming 期间稳定体的染色体,并且改进结果的 iPS 房间的质量。测试这个假设,我们在 iPS 期间为能减少的因素屏蔽了 DDR 信号房间正式就职。我们决定了那 Zscan4,在有 Yamanaka 因素的联合,不是仅仅显著地减少了 DDR 而且显著地支持了 iPS 房间产生的效率。Zscan4 的包括稳定了 genomic DNA,导致 p53 downregulation。而且, Zscan4 也提高了象通过 telomere 感染以后的 3 天一样早变长的 telomere 基于再结合的机制。作为结果,与 Zscan4 的增加产生的 iPS 房间比古典 iPS 房间展出了更长的 telomeres。惊人地,超过 50% iPS 房间线(11/19 ) 经由这生产了 “ Zscan4 protocol”产生了由 TCA 决定了的实时熊的 all-iPS 房间老鼠,与为用古典 Yamanaka 因素生产的线的 1/12 相比。我们的调查结果提供在 reprogramming 期间维持 genomic 稳定性支持的第一示范高质量的 iPS 房间的产生。 | Jing Jiang Wenjian Lv Xiaoying Ye Lingbo Wang Man Zhang Hui Yang Maja Okuka Chikai Zhou Xuan Zhang Lin Liu Jinsong Li | 2013 | Cell Research2013，23，1： | 14 |
| 10 | Xiaotan Sanjie decoction attenuates tumor angiogenesis by manipulating Notch-1-regulated proliferation of gastric cancer stem-like cells显示文摘AIM: To determine the underlying mechanisms of action and influence of Xiaotan Sanjie(XTSJ) decoction on gastric cancer stem-like cells(GCSCs). METHODS: The gastric cancer cell line MKN-45 line was selected and sorted by FACS using the cancer stem cell marker CD44; the stemness of these cells was checked in our previous study. In an in vitro study, the expression of Notch-1, Hes1, Vascular endothelial growth factor(VEGF), and Ki-67 in both CD44-positive gastric cancer stem-like cells(GCSCs) and CD44-negative cells was measured by Western blot. The effect of XTSJ serum on cell viability and on the above markers was measured by MTT assay and Western blot, respectively. In an in vivo study, the ability to induce angiogenesis and maintenance of GCSCs in CD44-positive-MKN-45- and CD44-negative-engrafted mice were detected by immunohistochemical staining using markers for CD34 and CD44, respectively. The role of XTSJ decoction in regulating the expression of Notch-1, Hes1, VEGF and Ki-67 was measured by Western blot and real-time polymerase chain reaction.RESULTS: CD44+ GCSCs showed more cell proliferation and VEGF secretion than CD44-negative cells in vitro, which were accompanied by the high expression of Notch-1 and Hes1 and positively associated with tumor growth(GCSCs vs CD44-negative cells, 2.72 ± 0.25 vs 1.46 ± 0.16, P < 0.05) and microvessel density(MVD)(GCSCs vs CD44-negative cells, 8.15 ± 0.42 vs 3.83 ± 0.49, P < 0.001) in vivo. XTSJ decoction inhibited the viability of both cell types in a dose-dependent manner in vitro. Specifically, a significant difference in the medium-(82.87% ± 6.53%) and high-dose XTSJ groups(77.43% ± 7.34%) was detected at 24 h in the CD44+ GCSCs group compared with the saline group(95.42% ± 5.76%) and the low-dose XTSJ group(90.74% ± 6.57%)(P < 0.05). However, the efficacy of XTSJ decoction was reduced in the CD44- groups; significant differences were only detected in the high-dose XTSJ group at 48 h(78.57% ± 6.94%) and 72 h(72.12% ± 7.68%) when compared with the other CD44- groups(P < 0.05). Notably, these differences were highly consistent with the Notch-1, Hes1, VEGF and Ki-67 expression in these cells. Similarly, in vivo, XTSJ decoction inhibited tumor growth in a dose-dependent manner. A significant difference was observed in the medium(1.76 ± 0.15) and high-dose XTSJ(1.33 ± 0.081) groups compared with the GCSCs control group(2.72 ± 0.25) and the low-dose XTSJ group(2.51 ± 0.25)(P < 0.05). We also detected a remarkable decrease of MVD in the medium-(7.10 ± 0.60) and high-dose XTSJ(5.99 ± 0.47) groups compared with the GCSC control group(8.15 ± 0.42) and the low-dose XTSJ group(8.14 ± 0.46)(P < 0.05). Additionally, CD44 expression was decreased in these groups [medium-(4.43 ± 0.45) and high-dose XTSJ groups(3.56 ± 0.31) vs the GCSC control(5.96 ± 0.46) and low dose XTSJ groups(5.91 ± 0.38)](P < 0.05). The significant differences in Notch-1, Hes1, VEGF and Ki-67 expression highly mirrored the results of XTSJ decoction in inhibiting tumor growth, MVD and CD44 expression.CONCLUSION: Notch-1 may play an important role in regulating the proliferation of GCSCs; XTSJ decoction could attenuate tumor angiogenesis, at least partially, by inhibiting Notch-1. | Bing Yan Long Liu Ying Zhao Li-Juan Xiu Da-Zhi Sun Xuan Liu Ye Lu Jun Shi Yin-Cheng Zhang Yong-Jin Li Xiao-Wei Wang Yu-Qi Zhou Shou-Han Feng Can Lv Pin-Kang Wei Zhi-Feng Qin | 2014 | World Journal of Gastroenterology2014，20，36： | 14 |
| 11 | DL-3-n-butylphthalide protects the blood-brain barrier against ischemia/hypoxia injury via upregulation of tight junction proteins显示文摘Background:The increased permeability of the blood-brain barrier (BBB) induced by ischemia/hypoxia is generally correlated with alteration of tight junctions (TJs). DL-3-n-butylphthalide (NBP) has been shown to exert neuroprotective effects after ischemic injury. However, few studies have assessed the correlation between NBP and TJs. This study aimed to investigate the potential effect of NBP on the TJ proteins claudin-5, zonula occludens-1 (ZO-1), and occludin during brain ischemia. Methods: A chronic cerebral hypoperfusion (CCH) Sprague-Dawley rat model was established, and NBP (20, 40, or 80 mg/kg, gavage, once a day) treatment was performed for 14 days. NBP (0.1 or 1.0μmol/L) pre-treatment was applied to an in vitro hypoxia microvascular endothelial cell model (1%〇2, 24 h). BBB permeability was assessed by performing the Evans blue assay. The expressions and localization of claudin-5, ZO-1, occludin, phosphorylated/total protein kinase B (p-Akt/Akt), phosphorylated/total glycogen synthase kinase 3p (GSK-3(3)/GSK-3p, and (3-catenin/p-actin were evaluated by Western blotting or immunofluorescence. Reactive oxygen species (ROS) generation was measured by flow cytometry analysis. TJ ultrastructure was observed by transmission electron microscopy. Results: In CCH rats, treatment with 40 and 80 mg/kg NBP decreased the Evans blue content in brain tissue (9.0 ± 0.9 (μg/g vs. 12.3 ± 1.9 (μg/g, P = 0.005;6.7 ± 0.6 μg/g vs. 12.3 ± 1.9μg/g, P < 0.01), increased the expression of claudin-5 (0.79 ± 0.08 mvs. 0.41 ± 0.06, P < 0.01;0.07 ± 0 .0 7 vs. 0.41 ± 0 .0 6 , P < 0 .61 ), and elevated the ZO-1 protein level (P < 0.05) in brain microvascular segments in a dose-dependent manner in comparison with the corresponding values in the model group. There was no significant difference in occludin expression (P > 0.05). In the hypoxia cell model, NBP pre-treatment improved TJ ultrastructure, decreased intracellular ROS level, and increased the expression of claudin-5 (P < 0.01) and ZO-1 (P < 0.01) in comparison with the corresponding values in the hypoxia group. NBP treatment also elevated the relative expression levels of p-Akt/Akt, p-GSK-3p/GSK-3β, and β-catenin/β-actin in comparison with the corresponding values in the hypoxia group (all P < 0 .0 5 ). Conclusion: NBP improves the barrier function of BBB against ischemic injury by upregulating the expression of TJ proteins, possibly by reducing oxidative stress and activating the Akt/GSK-3β/β-catenin signaling pathway. | Zhan-Ying Ye Han-Ying Xing Bei Wang Min Liu Pei-Yuan Lv | 2019 | Chinese Medical Journal2019，，11： | 12 |
| 12 | Biodiversity-based development and evolution:the emerging research systems in model and non-model organisms显示文摘Evolutionary developmental biology,or Evo-Devo for short,has become an established field that,broadly speaking,seeks to understand how changes in development drive major transitions and innovation in organismal evolution.It does so via integrating the principles and methods of many subdisciplines of biology.Although we have gained unprecedented knowledge from the studies on model organisms in the past decades,many fundamental and crucially essential processes remain a mystery.Considering the tremendous biodiversity of our planet,the current model organisms seem insufficient for us to understand the evolutionary and physiological processes of life and its adaptation to exterior environments.The currently increasing genomic data and the recently available gene-editing tools make it possible to extend our studies to non-model organisms.In this review,we review the recent work on the regulatory signaling of developmental and regeneration processes,environmental adaptation,and evolutionary mechanisms using both the existing model animals such as zebrafish and Drosophila,and the emerging nonstandard model organisms including amphioxus,ascidian,ciliates,single-celled phytoplankton,and marine nematode.In addition,the challenging questions and new directions in these systems are outlined as well. | Long Zhao Feng Gao Shan Gao Yujun Liang Hongan Long Zhiyi Lv Ying Su Naihao Ye Liusuo Zhang Chengtian Zhao Xiaoyu Wang Weibo Song Shicui Zhang Bo Dong | 2021 | Science China(Life Sciences)2021，64，8： | 9 |
| 13 | Tom70 serves as a molecular switch to determine pathological cardiac hypertrophy显示文摘病理学的心脏的肥大是心失败的不可避免的 forerunner。不管心脏的肥大的病原学, cardiomyocyte mitochondrial 改变总是在这上下文被观察。mitochondrial 先锋蛋白质的进口调节在 pathophysiological 下面维持 mitochondrial 功能的外部膜(汤姆) 建筑群管理的 mitochondrial 的 translocases;然而,它在病理学的心脏的肥大的发展的角色仍然保持不清楚。这里,我们证明 Tom70 是在从人和试验性的动物的病理学的 hypertrophic 心的 downregulated。在 Tom70 表示的减小在 vivo 并且在 vitro 生产了不同病理学的 cardiomyocyte 肥大。指向 Tom70 的眼的 atrophy-1 的有缺点的 mitochondrial 进口触发了细胞内部的氧化应力,它导致了病理学的细胞的回答。重要地,如果有到多样的 pro-hypertrophic 的完整的抵抗的 cardiomyocytes 侮辱,增加了 Tom70 层次。一起,这些结果表明 Tom70 充当安排 hypertrophic 压力和 mitochondrial 回答决定病理学的心脏的肥大的一个分子的开关。 | Jun Li Man Qi Changming Li Dan Shi Dasheng Zhang Duanyang Xie Tianyou Yuan Jing Feng Yi Liu Dandan Liang Xinran Xu Jinjin Chen Liang Xu Hong Zhang Jiangchuan Ye Fei LV Jian Huang Luying Peng Yi-Han Chen | 2014 | Cell Research2014，24，8： | 9 |
| 14 | Crustal Structure Revealed by a Deep Seismic Sounding Profile of Baijing-Gaoming-Jinwan in the Pearl River Delta显示文摘The Pearl River Estuary area, located in the middle part of the southern China coastal seismic belt, has long been considered a potential source of strong earthquakes above magnitude 7.0. To scientifically assess the potential strong earthquake risk in this area, a three-dimensional artificial seismic sounding experiment, consisting of a receiving array and seabed seismograph, was performed to reveal the deep crustal structure in this region. We used artificial ship-borne air-gun excitation shots as sources, and fixed and mobile stations as receivers to record seismic data from May to August 2015. This paper presents results along a line from the western side of the Pearl River Estuary to the western side of the Baijing-Gaoming-Jinwan profile. A two-dimensional velocity structure was constructed using seismic travel-time tomography. The inversion results show that the Moho depth is 27 km in the coastal area and 30 km in the northwest of the Pearl River Estuary area, indicating that the crust thins from land to sea. Two structural discontinuities and multiple low-velocity anomalies appear in the crustal section. Inside both discontinuity zones, a low-velocity layer, with a minimum velocity of 6.05 km s^(-1), exists at a depth of about 15 km, and another, with a minimum velocity of 6.37 km s^(-1), exists at a depth of about 21.5 km between the middle and lower crust. These low velocities suggest that the discontinuities may consist of partly molten material. Earthquakes with magnitudes higher than 5.0 occurred in the low-velocity layer along the profile. The deep Kaiping-Enping fault, rooted in the crust, may be one of the most important channels for deep material upwelling and is related to tectonic movement since the Cretaceous in the Pearl River Delta tectonic rift basin. | ZHANG Xiang YE Xiuwei LV Jinshui SUN Jinlong WANG Xiaona | 2018 | Journal of Ocean University of China2018，17，1： | 8 |
| 15 | Transjugular intrahepatic portosystemic shunt for pyrrolizidine alkaloid-related hepatic sinusoidal obstruction syndrome显示文摘BACKGROUND Treatments for hepatic sinusoidal obstruction syndrome(HSOS)are limited.AIM To evaluate transjugular intrahepatic portosystemic shunting(TIPS)as a treatment for pyrrolidine alkaloid-related HSOS(PA-HSOS).METHODS This retrospective analysis included patients with PA-HSOS admitted to the First Affiliated Hospital of the University of Science and Technology of China(June 2015 to January 2019).Baseline clinical characteristics and follow-up data were extracted from the medical records.All patients included in this study experienced failure of initial therapy.Patients were divided into the TIPS and conservative treatment groups according to the therapy they received.Liver function,maximal ascites depth,imaging characteristics,pathology findings,and survival were compared between groups.RESULTS The TIPS group included 37 patients(28 males),and the conservative treatment group included 17 patients(11 males).Baseline characteristics were similar between groups.There were two deaths in the TIPS group and seven deaths in the conservative treatment group during follow-up(3-48 mo).The 3-,6-,12-and 24-mo survival rates were 94.6%,94.6%,94.6%and 94.6%,respectively,in the TIPS group and 70.6%,57.8%,57.8%and 57.8%,respectively,in the conservative treatment group.Kaplan-Meier analysis revealed significantly longer survival for the TIPS group than for the conservative treatment group(P=0.001).Compared with the pre-treatment value,maximal ascites depth was significantly lower at 1 wk,2 wk,1 mo,and 3 mo for the TIPS group(all P<0.05)but not in the conservative treatment group.Contrast-enhanced computed tomography demonstrated the disappearance of patchy liver enhancement after TIPS.Pathology showed that liver congestion and hepatocyte swelling improved with time after TIPS placement.CONCLUSION TIPS may achieve better outcomes than conventional symptomatic treatment in patients with PA-HSOS. | Chun-Ze Zhou Rui-Feng Wang Wei-Fu Lv Yu-Qin Fu De-Lei Cheng Yi-Jiang Zhu Chang-Long Hou Xian-Jun Ye | 2020 | World Journal of Gastroenterology2020，26，24： | 7 |
| 16 | Apelin-13 inhibits lipoprotein lipase expression via the APJ/PKCα/miR-361-5p signaling pathway in THP-1 macrophage-derived foam cells显示文摘动脉粥样硬化患者损害被丰富的类脂化合物和长期的发炎的累积描绘。以前的研究显示了导出巨噬细胞的脂蛋白脂肪分解酵素(LPL ) 由加速类脂化合物累积和支持 inflammatory cytokine 分泌物支持动脉粥样硬化前进。尽管 apelin-13 被认为是一个 atheroprotective 因素,它是否能调整 LPL 的表示,仍然保持不清楚。这研究的目的是在 THP-1 导出巨噬细胞的泡沫房间在 LPL 和内在的机制的表示上探索 apelin-13 的效果。Apelin-13 显著地减少了在 10 和 100 nM 的集中的全部的胆固醇,免费胆固醇,和胆固醇酉旨的细胞的层次。ELISA 分析证实有 apelin-13 的处理减少了支持 inflammatory cytokine 分泌物,例如 interleukin-6 (IL-6 ) , interleukin-1 (IL-1 ) 和肿瘤坏死 factor-alpha (TNF-) 。apelin-13 禁止了由西方的污点和即时 PCR 分析揭示了的 LPL 的表示,这也被发现。生物信息学分析和双酶的记者试金直接显示了那 miR-361-5p downregulated 由指向 LPL 的 3UTR 的 LPL 的表示。另外, apelin-13 + miR-361-5p 显著地模仿 downregulated 在房间的 LPL 的表示。最后,我们表明了那 apelin-13 downregulated 通过激活 PKC 的活动的 LPL 的表示。一起拿,我们的结果显示出那 apelin-13 downregulated 经由激活在 THP-1 导出巨噬细胞的泡沫房间表明小径的 APJ/PKC/miR-361-5p 的 LPL 的表示,导致类脂化合物累积和支持 inflammatory cytokine 分泌物的抑制。因此,我们的研究由 apelin-13 提供重要新卓见进类脂化合物累积和支持 inflammatory cytokine 分泌物的抑制,并且在动脉粥样硬化作为一个有希望的治疗学的目标加亮 apelin-13。 | Xin Zhang Qiong Ye Duo Gong Yuan Lv Haipeng Cheng Chong Huang Lingyan Chen Zhenwang Zhao Liang Li Xie Wei Min Zhang Xiaodan Xia Xiaohua Yu Xilong Zheng Shuzhi Wang Zongbao Wang Chaoke Tang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，6： | 6 |
| 17 | A critical epitope in CD147 facilitates memory CD4^(+) T-cell hyper-activation in rheumatoid arthritis显示文摘The abnormal activation of CD4^(+)CD45RO+memory T(Tm)cells plays an important role in the pathogenesis of rheumatoid arthritis(RA).Previous studies have shown that CD147 participates in T-cell activation.However,it remains unclear whether CD147 is involved in abnormal Tm-cell activation in RA patients.In this study,we demonstrated that CD147 was predominantly upregulated in Tm cells derived from RA patients.The anti-CD147 mAb 5A12 specifically inhibited Tm-cell activation and proliferation and further restrained osteoclastogenesis.Using a structural–functional approach,we depicted the interface between 5A12 and CD147.This allowed us to identify two critical residues,Lys63 and Asp65,as potential targets for RA treatment,as the double mutation K63A/D65A inhibited Tm-cell activation,mimicking the neutralization by 5A12.This study provides not only a theoretical basis for a“CD147-Tm/Osteoclast-RA chain”for the potential prevention and treatment of RA or other T-cell-mediated autoimmune diseases but also a new target for related drug design and development. | Na Guo Sheng Ye Kui Zhang Xiaoling Yu Hongyong Cui Xiangmin Yang Peng lin Minghua Lv Jinlin Miao Yang Zhang Qing Han Rongguang Zhang Zhinan Chen Ping Zhu | 2019 | Cellular & Molecular Immunology2019，16，6： | 6 |
| 18 | Gingerol activates noxious cold ion channel TRPA1 in gastrointestinal tract显示文摘TRPA1 channels are non-selective cation channels that could be activated by plant-derived pungent products, including gingerol, a main active constituent of ginger. Ginger could improve the digestive function; however whether ginger improves the digestive function through activating TRPA1 receptor in gastrointestinal tract has not been investigated. In the present study, gingerol was used to stimulate cell lines(RIN14B or STC-1) while depletion of extracellular calcium.TRPA1 inhibitor(rethenium red) and TRPA1 gene silencing via TRPA1-specific si RNA were also used for mechanistic studies. The intracellular calcium and secretion of serotonin or cholecystokinin were measured by fura-2/AM and ELISA. Stimulation of those cells with gingerol increased intracellular calcium levels and the serotonin or cholecystokinin secretion. The gingerol-induced intracellular calcium increase and secretion(serotonin or cholecystokinin) release were completely blocked by ruthenium red, EGTA, and TRPA1-specific si RNA. In summary, our results suggested that gingerol derived from ginger might improve the digestive function through secretion releasing from endocrine cells of the gut by inducing TRPA1-mediated calcium influx. | YANG Meng-Qi YE Lin-Lan LIU Xiao-Ling QI Xiao-Ming LV Jia-Di WANG Gang FARHAN Ulah-Khan WAQAS Nawaz CHEN Ding-Ding HAN Lei ZHOU Xiao-Hui | 2016 | Chinese Journal of Natural Medicines2016，14，6： | 6 |
| 19 | Nitazoxanide,an anti-parasitic drug,efficiently ameliorates learning and memory impairments in AD model mice显示文摘The pathogenesis of Alzheimer's disease(AD)is characterized by both accumulation ofβ-amyloid(AB)plaque and formation of neurofirillary tangles in the brain.Recent evidence shows that autophagy activation may potently promote intraellular Aβclearance.Thus targeting autophagy becomes a promising strategy for discovery of drug leads against AD.In the present study,we established a platform to discover autophagy stimulator and screened the lab in-house FDA-approved drug library.We found thatanti-parasitic drug nitazoxanide(NTZ)was an autophagy activator and could efficiently improve learning and memory impairmentsin APP/PS1 transgenic mice.In BV2 cells and primary cortical astrocytes,NTZ stimulated autophagy and promoted Aβclearance by inhibiting both P12K/AKT/mTOR/ULK1 and NQO1/mTOR/ULK1 signaling pathways;NTZ treatment attenuated LPS-induced inflammation by inhibiting PI3K/AKT/IKB/NFKB signaling.In SH-SY5Y cells and primary cortical neurons,NTZ treatment restrained tau hyperphosphorylation through inhibition of PI3K/AKT/GSK3βpathway.The beneficial effects and related signaling mechanisms from the in vitro studies were also observed in APP/PS1 transgenic mice following administration of NTZ(90 mg·kg^-1d,ig)for100 days.Furthermore,NTZ administration decreased Aβlevel and senile plaque formation in the hippocampus and cerebral cortexof APP/PS1 transgenic mice,and improved learning and memory impairments in Morris water maze assay.In conclusion,our results highlight the potential of NTZ in the treatment of AD. | Lei Fan Xiao-xia Qiu Zhi-yuan Zhu Jian-lu Lv Jian Lu Fei Mao Jin Zhu Jia-ying Wang Xiao-wei Guan Jing Chen Jin Ren Ji-ming Ye Yong-hua Zhao Jian Li Xu Shen | 2019 | Acta Pharmacologica Sinica2019，40，10： | 6 |
| 20 | Impacts of Drought and Human Activity on Vegetation Growth in the Grain for Green Program Region,China显示文摘The Chinese government adopted six ecological restoration programs to improve its natural environments. Although these programs have proven successful in improving local environments, some studies have questioned their performance when regions suffer from drought. Whether we should consider the effects of drought on vegetation change in assessments of the benefits of ecological restoration programs is unclear. Therefore, taking the Grain for Green Program(GGP) region as a study area, we estimated vegetation growth in the region from 2000–2010 to clarify the trends in vegetation and their driving forces. Results showed that: 1) vegetation growth increased in the GGP region during 2000–2010, with 59.4% of the area showing an increase in the Normalized Difference Vegetation Index(NDVI). This confirmed the benefits of the ecological restoration program. 2) Drought can affect the vegetation change trend, but human activity plays a significant role in altering vegetation growth, and the slight downward trend in the NDVI was not consistent with the severity of the drought. Positive human activity led to increased NDVI in 89.13% of areas. Of these, 22.52% suffered drought, but positive human activity offset the damage in part. 3) Results of this research suggest that appropriate human activity can maximize the benefits of ecological restoration programs and minimize the effects of extreme weather. We therefore recommend incorporating eco-risk assessment and scientific management mechanisms in the design and management of ecosystem restoration programs. | WANG Hao LIU Guohua LI Zongshan YE Xin FU Bojie LV Yihe | 2018 | Chinese Geographical Science2018，28，3： | 6 |
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