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| 1 | Extraction and purification of the polysaccharides in Hippohaere rhamnoides L.显示文摘The different extraction technology and purification technology of Hippohpae rhamoides polysaccharides were researched in the paper.The best method of papain extraction were obtained,the ratio of papain 2%,pH at 5.5,temperature at 45℃and extraction time of 20 min were suitable for papain extraction.The highest content of Hippohpae rhamoides polysaccharides was 44.28 mg·g^(-1). The optimum process of ultrasonic extraction were obtained,namely extracted for 55 min at 480 W with the material ratio of 1:20. The highest content of Hippohpae rhamoides polysaccharides was 48.63 mg·g^(-1).The results showed that the ultrasonic and papain extraction together was the best method,the content was 54.30 mg·g^(-1).After the removing protein,pigment and dialysis.Two fraction were separated from the purified Hippohpae rhamoides by DEAE-cellulose chromatography,the main fraction was collected finally. The fraction was identified by Sepharose CL-4B gel filtration.Ultraviolet spectrometry,freeze-thawing analysis showed that fraction was purified.Its molecular weight was probably 109.4 ku. | XU Yaqin JIN Ting YU Zeyuan FU Hong | 2007 | Journal of Northeast Agricultural University(English Edition)2007，14，2： | 3 |
| 2 | The flexural property and its synergistic mechanism of multibody molded beetle elytron plates显示文摘To improve the applications of beetle elytron plates(BEPs,which are biomimetic sandwich plates inspired by beetle elytra),the flexural performance and its synergistic mechanism of multibody molded BEPs were investigated via cantilever testing and finite element method(FEM).The results are summarized as follows.(1)Although debonding damage causes failure of the multibody molded BEPs and honeycomb plate and the reasonable range of trabecular size for BEPs is narrow,both the optimal loading capacity per mass and failure deformation of the BEPs are over two times those of the honeycomb plate.(2)A flexural synergistic mechanism is revealed in the trabecular-honeycomb core structure of BEPs;this mechanism causes the maximum deformation of core structure to gradually transfer from the honeycomb wall to the trabeculae with the increase inη(the ratio of the trabecular radius to the distance between the center points of two trabeculae),which means the different stretching behaviors in these core structures.(3)Unlike the compressive mechanism of BEPs,by controlling and balancing the deformation degrees of the trabeculae and honeycomb walls,the flexural mechanism achieves a minimum core deformation and an optimal flexural performance.These results suggest a qualitative relationship between the deformation behavior of trabecular-honeycomb core structure and bending performance of the whole BEP,and provide a solid foundation for subsequent research and the considerable application potential of this biomimetic sandwich structure in many fields. | YU XinDi ZHANG XiaoMing CHEN JinXiang ZHAO CaiQi ZHAO TiDong FU YaQin | 2020 | Science China(Technological Sciences)2020，63，5： | 2 |
| 3 | Self-assembled organic-inorganic composite superlattiee thin films in- corporating photo-and electro-chemically active phosphomolybdate anion 显示文摘 | Zhang Tierui Feng Wei Fu Yaqin | 2002 | Journal of Materials Chemistry2002，12，： | 1 |
| 4 | Novel testing equipment for tabric wrinkle resistance simulating actual wear显示文摘 | LIU Chengxia FU Yaqin WU Niying | 2014 | Textile Research Journal2014，84，10： | 1 |
| 5 | Abrasion resistance of biaxially oriented polypropylene films coated with nanocomposite hard coatings显示文摘 | Wang Jing Zhu Yaofeng Fu Yaqin | 2013 | Applied Surface Science2013，，： | 1 |
| 6 | Correction to:Efficient derivation of extended pluripotent stem cells from NOD-scid II2rg^-/-mice显示文摘Figure 1.Generation of NOD-scid II2rg^-1-extended pluripotent stem cells.(A)Schematic of two approaches used for generating NOD-scid II2rg^-1-extended pluripotent stem cells:de novo derivation from blastocysts(upper panels)and chemical reprogramming from embryonic fibroblasts(lower panels).(B)Phase-contrast images of de novo derived outgrowth and EPS colonies for 17 passages in LCDM medium.Scale bars,100 pm.(C)qRT-PCR analysis of XEN marker genes expression during the chemical induction process(day 16).Error bars indicate SEM(n=2).(D)Co-immunostaining of XEN marker genes during the chemical induction process(day 16).Upper panels:GATA6 and SALL4;lower panels:SOX17 and SALL4.Scale bars,100 pm. | Yaqin Du Ting Wang Jun Xu Chaoran Zhao Haibo Li Yao Fu Yaxing Xu Liangfu Xie Jingru Zhao Weifeng Yang Ming Yin Jinhua Wen Hongkui Deng | 2019 | Protein & Cell2019，10，2： | 1 |
| 7 | Shape memory effact and mechanical properties of carbon nanotube/shape memory polymer nanocomposites 显示文摘 | Ni Qingqing Zhang Chunsheng Fu Yaqin | 2007 | Composite Structures2007，81，2： | 1 |
| 8 | Shape memory effect and mechanical properties of carbon nano tube/shape memory polymer nanocomposites显示文摘 | Ni Qingqing Zhang Chunsheng Fu Yaqin | 2007 | Compos Struct2007，81，2： | 1 |
| 9 | High molecular weight dendronized poly (fluorene)s with peripheral carbazole groups synthesis, characterization, and properties 显示文摘 | Yaqin Fu Yi Li Zhishan Bo | 2004 | Macromolecules2004，37，17： | 1 |
| 10 | Rod-like crystal growth of dioctyl substituted polyfluorene from nematic and isotropic states显示文摘 | ZHOU Jianjun LI Jing FU Yaqin | 2007 | Polymer2007，48，： | 1 |
| 11 | Vibration properties and transverse shear characteristics of multibody molded beetle elytron plates显示文摘This study utilized cantilever experiments to investigate the vibration properties of multibody molded beetle elytron plates(BEPs),which is a type of biomimetic sandwich plate inspired by beetle elytra;the corresponding shear characteristics were further revealed by a finite element method(FEM). The following results were obtained:(1) Experimental results suggest that the maximum displacement response of the BEPs was about 25% less than that of a honeycomb plate with almost the same first natural frequency,which indicates that a BEP with reasonable structural parameters has the potential to replace a honeycomb plate to achieve better vibration performance;(2) The trabecular structure not only enhanced the shear stiffness of the core layer in column areas but also the skins in the honeycomb wall areas,thus changing the distribution of the shear force in the different components and improving the mechanical performances of the BEP;and(3) Although this enhancement effect from trabeculae was not uniform,the average shear force proportion of the skins(or core structure) in the entire BEP structure was very close to that of the honeycomb plate. Therefore,the shear calculation assumption used for honeycomb plates is still applicable in the BEP.The results provide an experimental basis for the design and application of BEPs and inspiration for the development of related products in vibrational environments. | ZHANG XiaoMing YU XinDi CHEN JinXiang PAN LongCheng HU LiPing FU YaQin | 2020 | Science China(Technological Sciences)2020，63，12： | 1 |
| 12 | Shape memory effect and mechanical properties of carbon nanotube/shape memory polymer nanoeomposites 显示文摘 | Ni Qingqing Zhang Chunsheng Fu Yaqin | 2007 | Compos Struet2007，81，2： | 1 |
| 13 | Self-assembly of various Au nanocrystals on functionalized water-stable PVA/PEI nanofibers: A highly efficient surface-enhanced Raman scattering substrates with high density of “hot” spots显示文摘 | Han Zhu MingLiang Du Ming Zhang Pan Wang ShiYong Bao Meiling Zou YaQin Fu JuMing Yao | 2014 | Biosensors and Bioelectronics2014，，： | 1 |
| 14 | Complete synchroniza-tion and stability of star-shaped complex networks 显示文摘 | Gu Yaqin Shao Chun Fu Xinchu | 2006 | Chaos Solitons & Fractals2006，28，2： | 1 |
| 15 | Plant and animal positive-sense single-stranded RNA viruses encode small proteins important for viral infection in their negative-sense strand显示文摘Positive-sense single-stranded RNA(+ssRNA)viruses,the most abundant viruses of eukaryotes in nature,require the synthesis of negative-sense RNA(-RNA)using their genomic(positive-sense)RNA(+RNA)as a template for replication.Based on current evidence,viral proteins are translated via viral+RNAs,whereas-RNA is considered to be a viral replication intermediate without coding capacity.Here,we report that plant and animal+ssRNA viruses contain small open reading frames(ORFs)in their-RNA(reverse ORFs[rORFs]).Using turnip mosaic virus(TuMV)as a model for plant+ssRNA viruses,we demonstrate that small proteins encoded by rORFs display specific subcellularlocalizations,and confirm the presence of rORF2 in infected cells through mass spectrometry analysis.The protein encoded by TuMV rORF2 forms punctuate granules that are localized in the perinuclear region and co-localized with viral replication complexes.The rORF2 protein can directly interact with the viral RNA-dependent RNA polymerase,and mutation of rORF2 completely abolishes virus infection,whereas ectopic expression of rORF2 rescues the mutant virus.Furthermore,we show that several rORFs in the-RNA of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)have the ability to suppress type l interferon production and facilitate the infection of ve-sicular stomatitis virus.In addition,we provide evidence that TuMV might utilize internal ribosome entry sites to translate these small rORFs.Taken together,these findings indicate that the-RNA of+ssRNA vi-ruses can also have the coding capacity and that small proteins encoded therein play critical roles in viral infection,revealing a viral proteome larger than previously thought. | Pan Gong Qingtang Shen Mingzhen Zhang Rui Qiao Jing Jiang Lili Su Siwen Zhao Shuai Fu Yu Ma Linhao Ge Yaqin Wang Rosa Lozano-Durán Aiming Wang Fangfang Li Xueping Zhou | 2023 | Molecular Plant2023，16，11： | 0 |
| 16 | Neural stem cell-derived exosomes regulate cell proliferation,migration,and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia显示文摘Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 axis.However,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain unknown.Therefore,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 axis.Methods:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on BMECs.Results:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic conditions.The overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic conditions.Moreover,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell death.Hes1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell death.Hyperemic structures were observed in the regions of the hippocampus and cortex in hypoxia-induced mice.Meanwhile,EXO treatment improved cerebrovascular alterations.Conclusion:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic conditions.Therefore,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury. | Xiaojun Deng Xiaoyi Hu Shang Wang Hui Zhao Yaqin Wei Jiaqi Fu Wenhui Wu Jinming Liu Caicai Zhang Lili Wang Ping Yuan | 2024 | Animal Models and Experimental Medicine2024，7，1： | 0 |
| 17 | Influence of the Chamfer on the Flexural Properties of Beetle Elytron Plates显示文摘To improve the flexural properties of Beetle Elytron Plates(BEPs)and clarify the effect of the transition arcs(chamfers)between the skins and the trabeculae,the chamfers were set in BEPs,and then the influence of the chamfer on BEPs'mechanical properties was investigated via experimentation and the Finite Elemnent Method simulation(FEM).The results indicate that the influence of the chamfer on the flexural properties and ductility was most obvious in the Trabecular Beetle Elytron Plates(TBEPs),less obvious in the Honeycomb Plates(HPs)and basically no efiect was observed on End-trabecular Beetle Elytron Plates(EBEPs).The chamfer can improve the mechanical stability of EBEPSs.As the chamfer diameter increased in the BEPs,the length of the residual trabecular root on the skin increased when failure occurred in the TBEPs.The crack position in the honeycomb wallsof the HPs gradually shifted from the skin to the center.The EBEPs continued to exhibit oblique cracks.From the perspective of the force characteristics of these BEPs.combined with numerical simulation,the influence mechanism of the chamfer on their flcxural propertics was investigated. | Ning Hao Mengye Xu Jinxiang Chen Yiheng Song Zhijie Zhang Yaqin Fu | 2021 | Journal of Bionic Engineering2021，18，1： | 0 |
| 18 | Comparison of the Minimum Bounding Rectangle and Minimum Circumscribed Ellipse of Rain Cells from TRMM显示文摘Based on the TRMM dataset, this paper compares the applicability of the improved MCE(minimum circumscribed ellipse), MBR(minimum bounding rectangle), and DIA(direct indexing area) methods for rain cell fitting. These three methods can reflect the geometric characteristics of clouds and apply geometric parameters to estimate the real dimensions of rain cells. The MCE method shows a major advantage in identifying the circumference of rain cells. The circumference of rain cells identified by MCE in most samples is smaller than that identified by DIA and MBR, and more similar to the observed rain cells. The area of rain cells identified by MBR is relatively robust. For rain cells composed of many pixels(N> 20), the overall performance is better than that of MCE, but the contribution of MBR to the best identification results,which have the shortest circumference and the smallest area, is less than that of MCE. The DIA method is best suited to small rain cells with a circumference of less than 100 km and an area of less than 120 km^(2), but the overall performance is mediocre. The MCE method tends to achieve the highest success at any angle, whereas there are fewer “best identification”results from DIA or MBR and more of the worst ones in the along-track direction and cross-track direction. Through this comprehensive comparison, we conclude that MCE can obtain the best fitting results with the shortest circumference and the smallest area on behalf of the high filling effect for all sizes of rain cells. | Hongke CAI Yaqin MAO Xuanhao ZHU Yunfei FU Renjun ZHOU | 2024 | Advances in Atmospheric Sciences2024，41，3： | 0 |
| 19 | Electron modulation by atomic Ir site decoration in porous Co/N co-doped carbon for electrocatalytic hydrogen evolution显示文摘The rational design of materials at atomic scale as efficient and stable electrocatalysts for hydrogen evolution reaction(HER)is critical for energy conversion.Herein,we report a novel hybrid nanostructure with iridium(Ir)and cobalt(Co)atomic pair configuration anchored in porous nitrogen-doped carbon(pNC)nanosheets(denoted as IrCo-pNC)for electrocatalytic HER.Experimental investigations and theoretical calculations reveal that the interaction between Ir and Co species in pNC promotes electron accumulation and depletion around isolated Ir and Co atoms,respectively,resulting in a local asymmetry electron density distribution.Density functional theory calculations also suggest that the electrons transfer from Co to adjacent Ir atom causing the down shift of the d-band center of Ir 5d in IrCo-pNC catalyst,thus optimizing the adsorption of hydrogen on Ir sites.The as-prepared IrCo-pNC exhibits significant HER performance with an overpotential of 21 mV to achieve a current density of 10 mA·cm^(−2)in 0.5 M H2SO4.This work provides insight into the role of asymmetry electron density distribution in nanomaterials in regulating HER electrocatalysis. | Youkui Zhang Zitong Yan Yeli Gao Yaqin Fu Wenhao Li Yunxiang Lin Chuanqiang Wu Yujuan Pu Tao Duan Li Song | 2023 | Nano Research2023，16，2： | 0 |
| 20 | Influence Mechanism of the Trabecular and Chamfer Radii on the Three-point Bending Properties of Trabecular Beetle Elytron Plates显示文摘To improve the mechanical properties of Trabecular Beetle Elytron Plates(TBEPs,a type of biomimetic sandwich structure inspired by the beetle elytron)under transverse loads,three-point bending tests are performed to investigate the influence of the trabecular and chamfer radii of the core structure on the mechanical performance of TBEPs manufactured by 3D printing technology.The results show that the three-point bending performance of TBEPs can be improved by setting reasonable trabecular and chamfer radii;however,excessive increases in these radii can cause a decline in the mechanical performance.For the reason,these two structural parameters can enhance the deformation stiffness of the whole structure and the connection property between the core and skin,which is also the mechanical reason why Prosopocoilus inclinatus beetle elytra have thick,short trabeculae with a large chamfer radius.However,when these radii increase to a certain extent,the cracks are ultimately controlled between two adjacent trabeculae,and the failure of the plate is determined by the skin rather than the core structure.Therefore,this study suggests a reasonable range for trabecular and chamfer radii,and indicates that TBEPs are better suited for engineering applications that have high compression requirements and general bending requirements. | Xiaoming Zhang Xindi Yu Jinxiang Chen Caiqi Zhao Sujun Guan Yaqin Fu | 2021 | Journal of Bionic Engineering2021，18，2： | 0 |
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