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| 1 | Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development显示文摘Brain size and cognitive skills are the most dramatically changed traits in humans during evolution and yet the genetic mechanisms underlying these human-specific changes remain elusive.Here,we successfully generated 11 transgenic rhesus monkeys(8 first-generation and 3 second-generation)carrying human copies of MCPH1,an important gene for brain development and brain evolution.Brain-image and tissue-section analyses indicated an altered pattern of neural-cell differentiation,resulting in a delayed neuronal maturation and neural-fiber myelination of the transgenic monkeys,similar to the known evolutionary change of developmental delay(neoteny)in humans.Further brain-transcriptome and tissue-section analyses of major developmental stages showed a marked human-like expression delay of neuron differentiation and synaptic-signaling genes,providing a molecular explanation for the observed brain-developmental delay of the transgenic monkeys.More importantly,the transgenic monkeys exhibited better short-term memory and shorter reaction time compared with the wild-type controls in the delayed-matching-to-sample task.The presented data represent the first attempt to experimentally interrogate the genetic basis of human brain origin using a transgenic monkey model and it values the use of non-human primates in understanding unique human traits. | Lei Shi Xin Luo Jin Jiang Yongchang chen Cirong Liu Ting Hu Min Li Qiang Lin Yanjiao Li Jun Huang Hong Wang Yuyu Niu Yundi Shi Martin Styner Jianhong Wang Yi Lu Xuejin Sun Hualin Yu Weizhi Ji Bing Su | 2019 | National Science Review2019，6，3： | 12 |
| 2 | Effects of Dietary Stachyose on Growth Performance, Digestive Enzyme Activities and Intestinal Morphology of Juvenile Turbot(Scophthalmus maximus L)显示文摘A 12-week feeding trial was conducted to evaluate the effects of dietary stachyose on the growth performance, digestive enzymes activities and intestinal structures of juvenile turbot(Scophthalmus maximus L). Five isonitrogenous(49.58% crude protein) and isolipidic(10.50% crude lipid) diets were formulated to contain 0(Control), 0.625%(S-0.625), 1.25%(S-1.25), 2.5%(S-2.5) and 5%(S-5) stachyose, respectively. With the increase of stachyose level, the growth performance and feed utilization of turbot, such as the specific growth rate, final mean body weight, weight gain rate and feed efficiency, increased significantly(P < 0.05) and then stabilized. The feed intake of fish fed S-5 was significantly higher(P < 0.05) than that of fish in other groups. The activities of trypsin, intestinal caseinolytic, stomach and intestinal amylase were significantly influenced by stachyose(P < 0.05). The highest values of trypsin and intestinal caseinolytic activities were observed in group S-1.25, while the highest activity of stomach amylase and the lowest activity of intestine amylase were observed in group S-5. No lesion or damage was found on the distal intestine structures of fish from all treatments, while the height of simple folds in the distal intestine was significantly increased(P< 0.05) when 1.25% or 2.5% stachyose was added in the diets. These results indicated that moderate level of stachyose(1.25%) improves the growth performance, feed utilization, digestive enzyme activities and the distal intestine structures of juvenile turbot. | HU Haibin ZHANG Yanjiao MAI Kangsen AI Qinghui XU Wei ZHANG Wenbing LI Yanxian LIU Jintao | 2015 | Journal of Ocean University of China2015，14，5： | 10 |
| 3 | Generation of obese rat model by transcription activator-like effector nucleases targeting the leptin receptor gene显示文摘The laboratory rat is a valuable mammalian model organism for basic research and drug discovery. Here we demonstrate an efficient methodology by applying transcription activator-like effector nucleases(TALENs) technology to generate Leptin receptor(Lepr) knockout rats on the Sprague Dawley(SD) genetic background. Through direct injection of in vitro transcribed m RNA of TALEN pairs into SD rat zygotes, somatic mutations were induced in two of three resulting pups. One of the founders carrying bi-allelic mutation exhibited early onset of obesity and infertility. The other founder carried a chimeric mutation which was efficiently transmitted to the progenies. Through phenotyping of the resulting three lines of rats bearing distinct mutations in the Lepr locus, we found that the strains with a frame-shifted or premature stop codon mutation led to obesity and metabolic disorders. However, no obvious defect was observed in a strain with an in-frame 57 bp deletion in the extracellular domain of Lepr. This suggests the deleted amino acids do not significantly affect Lepr structure and function. This is the first report of generating the Lepr mutant obese rat model in SD strain through a reverse genetic approach. This suggests that TALEN is an efficient and powerful gene editing technology for the generation of disease models. | Yuting Chen Wenqing Lu Na Gao Yi Long Yanjiao Shao Meizhen Liu Huaqing Chen Shixin Ye Xueyun Ma Mingyao Liu Dali Li | 2017 | Science China(Life Sciences)2017，60，2： | 4 |
| 4 | Psychometric properties of the Caregiver Strain Questionnaire among Chinese parents of children with ADHD or ASD显示文摘Background There is an urgent need in clinical practice to measure the stress of parenting.The Caregiver Strain Questionnaire(CGSQ)was found to be useful to measure parenting stress,but it has not been validated among the Chinese population.Aims To assess the reliability and construct validity of the Chinese version of CGSQ among Chinese parents.Methods From 2016 to 2017,266 parents(patient group)with a child having DSM-5-defined attention deficit hyperactivity disorder(ADHD)(n=107)or autism spectrum disorder(ASD)(n=159)and 268 parents of healthy children(control group)were recruited to the present study in Kunming,Yunnan province.All the parents were asked to fill out the Chinese version of CGSQ.We conducted exploratory factor analysis and confirmatory factor analysis(CFA)to verify construct validity of CGSQ in both patient and control groups.Cronbach’s α coefficient as an index of internal consistency was assessed for each subscale.Fourteen days later,23 subjects filled out the scale again.Intra-class correlation coefficient was calculated to evaluate the test-retest reliability.Results(1)Cronbach’s alpha of the global scale was 0.901 for the control group and 0.952 for the patient group.The test-retest reliability for the whole scale was 0.890;(2)CFA indicated that the three-factor model had better fitting indices compared with the two-factor model in both groups.Besides,the fitting indices in the patient group were more favourable than those of the control group,withχ2/df=1.564,Goodness-of Fit Index=0.841,Comparative Fit Index=0.954,and root mean square error of approximation=0.065 for the patient group at three-factor model;(3)The caregiver strain of ASD parents was statistically higher than that of ADHD parents,and caregiver strain of ADHD parents was higher than that of control group.Conclusion These findings provide initial evidence to support the construct validity and reliability of CGSQ as a parenting stress measurement tool for Chinese parents,especially for parents of children with ADHD or ASD. | Runxu Yang Huizhi Zhou Jin Liu Yufeng Wang Yan Zhang Yanjiao Wang Ruixiang Liu Xia Cao Dong Han Shuran Yang Jianzhong Yang Chuanyuan Kang | 2021 | General Psychiatry2021，34，2： | 2 |
| 5 | High-strength hydrogel as a reusable adsorbent of copper ions显示文摘 | Wang Ning Han Yanjiao Liu Yuan | 2012 | Journal of Hazardous Materials2012，，： | 1 |
| 6 | CDC20,TOP2A and NEK2 Expression in Esophageal Squamous Cell Carcinoma and Its Clinical Significance显示文摘Objective:to study the expression and clinical significance CDC20,TOP2A,NEK2 esophageal squamous cell carcinoma.Methods:To select 70 patients with esophageal squamous cell carcinoma,Between August 2018-August 2020,All intraoperative pathological specimens,A group-35 cases),Cancer tissue,B group,adjacent tissues),two groups of CDC20,TOP2A,NEK 2 expression were detected and analyzed by immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction-RT-PcR)assay.Results:the values of CDC20,TOP2A,NEK2 expression level in A group were significantly higher than those in B group-P<0.05).The expression level CDC20,TOP2A,NEK2 esophageal squamous cell carcinoma was positively correlated with TNM stage and lymphatic metastasis,and negatively correlated with tumor differentiation.Conclusion:CDC20,TOP2A,NEK2 high expression level directly affects the metastasis,recurrence and prognosis of esophageal squamous cell carcinoma.The combination of three indexes can accurately evaluate the pathological status of patients with esophageal squamous cell carcinoma and help to judge the prognosis of patients accurately. | Yanjiao Liu Longying He Qian Zhang Xinglong Zhang | 2020 | Journal of Oncology Research2020，2，2： | 1 |
| 7 | Prediction of Human Drug Absorption Using Liposome Electrokinetic Chromatography显示文摘 | Yongjun Wang Jin Sun Hongzhuo Liu Yanjiao Wang Zhonggui He | 2007 | Chromatographia (-)2007，，3： | 1 |
| 8 | Synthesis and gas sensing properties of hierarchical meso-macroporous SnO 2 for detection of indoor air pollutants显示文摘 | Huihua Li Fanli Meng Jinyun Liu Yufeng Sun Zhen Jin Lingtao Kong Yanjiao Hu Jinhuai Liu | 2012 | Sensors & Actuators: B. Chemical2012，，： | 1 |
| 9 | Evaluation of Reprocessed Fengyun-3B Global Outgoing Longwave Radiation Data:Comparison with CERES OLR显示文摘Outgoing longwave radiation(OLR)at the top of the atmosphere(TOA)is a key parameter for understanding and interpreting the relationship between clouds,radiation,and climate interactions.It has been one of the operational products of the Fengyun(FY)meteorological satellites.OLR accuracy has gradually improved with advancements in satellite payload performance and the OLR retrieval algorithm.Supported by the National Key R&D Program Retrospective Calibration of Historical Chinese Earth Observation Satellite data(Richceos)project,a long-term OLR climate data record(CDR)was reprocessed based on the recalibrated Level 1 data of FY series satellites using the latest OLR retrieval algorithm.In this study,Fengyun-3B(FY-3B)’s reprocessed global OLR data from 2010 to 2018 were evaluated by using the Clouds and the Earth’s Radiant Energy System(CERES)global daily OLR data.The results showed that there was a high consistency between the FY-3B instantaneous OLR and CERES Single Scanner Footprint(SSF)OLR.Globally,between the two CDR datasets,the correlation coefficient reached 0.98,and the rootmean-square error(RMSE)was approximately 8-9 W m^(−2).The bias mainly came from the edge regions of the satellite orbit,which may be related to the satellite zenith angle and cloud cover distribution.It was shown that the longterm FY-3B OLR had temporal stability compared to CERES OLR long-term data.In terms of spatial distribution,the mean deviations showed zonal and seasonal characteristics,although seasonal fluctuations were observed in the differences between the two datasets.Effects of FY-3B OLR application to the South China Sea monsoon region and ENSO were demonstrated and analyzed,and the results showed that the seasonal deviation of FY-3B’s OLR comes mainly from the retrieval algorithm.However,it has little effect on the analysis of climate events. | Wanchun ZHANG Jian LIU Peng ZHANG Ling SUN Hanlie XU Yanjiao WANG Lin CHEN | 2022 | Journal of Meteorological Research2022，36，3： | 0 |
| 10 | LncRNA_003991 regulates cerebral ischemia/reperfusion injury via acting as a ceRNA For miRNA874-3p to target GSDMD显示文摘Ischemic stroke(IS)is a major disease threatening human health,and reperfusion therapy are necessary.However,this can cause ischemia/reperfusion(I/R)injury,and the mechanism is complicated.Pyroptosis is an inflammation related programmed cell death mode between necrosis and apoptosis,which plays an essential role in the pathological process of I/R injury.Competitive endogenous RNA(ceRNA)has been proven to be an essential regulatory mechanism in many diseases. | Wei Liu Wei Ma Li Chunyan Liu Kuangpin Liu Jie Li Yanjiao Zhang Sijia Yang Jinwei Guo Jianhui Li Liyan | 2021 | 解剖学杂志2021，44，S01： | 0 |
| 11 | Expression of CDC20, TOP2A in Esophageal Phosphorus Carcinoma and Its Relationship with Survival of Esophageal Carcinoma显示文摘Objective:To study the expression of CDC20,TOP2A in esophageal squamous cell carcinoma and its relationship with survival of esophageal carcinoma.Methods:65 patients with esophageal squamous cell carcinoma from January 2016 to June 2018 were selected by computer random selection.All patients were treated with radical operation.The CDC20,TOP2A expression of the patients was examined.At the same time,the relationship between 3-year survival rate and CDC20,TOP2A was analyzed by follow-up investigation.Results:the CDC20,TOP2A expression level of cancer tissue group was higher than that of adjacent tissue group and normal tissue group(P<0.05),and the CDC20,TOP2A expression level of adjacent tissue group was higher than that of normal tissue group(P<0.05).There was no significant difference in sex,age,tumor size,tumor location and CDC20,TOP2A expression level in patients with esophageal squamous cell carcinoma,P>0.05;there were differences between groups(P P>0.05)and positive proportion in TNM stage,lymphatic metastasis,invasion factor and CDC20,TOP2A expression level;there were differences in tumor differentiation,5-year survival rate and CDC20,TOP2A expression level(P P>0.05),and showed inverse proportion relationship.Conclusion:metastasis,recurrence and prognosis of esophageal squamous cell carcinoma are related to the level of CDC20,TOP2A expression.These two indexes can effectively evaluate the pathological situation of esophageal cancer and provide an important reference for the prognosis of esophageal squamous cell carcinoma patients. | Yanjiao Liu Longying He Qian Zhang Xinglong Zhang | 2020 | Journal of Integrative Medicine(双语)2020，9，2： | 0 |
| 12 | Dual-mode distributed feedback quantum cascade laser based on stacked 3D monolithic integration for on-chip multi-channel gas sensing显示文摘To facilitate the development of on-chip integrated mid-infrared multi-channel gas sensing systems,we propose a high-power dual-mode(7.01 and 7.5μm)distributed feedback quantum cascade laser based on stacked 3D monolithic integration.Longitudinal mode control is achieved by preparing longitudinal nested bi-periodic compound one-dimensional Bragg gratings along the direction of the cavity length in the confinement layer.Additionally,transverse coherent coupling ridges perpendicular to the cavity length direction are fabricated in the upper waveguide layer to promote the fundamental transverse mode output when all ridges are in phase.Stable dual-wavelength simultaneous emission with a side-mode suppression ratio of more than 20 dB was achieved by holographic exposure and wet etching.The entire spectral tuning range covers nearly 100 nm through joint tuning of the injection current and heat-sink temperature.High peak power and beam quality are guaranteed by the parallel coherent integration of seven-element ridge arrays.The device operates in a fundamental supermode with a single-lobed far-field pattern,and its peak output power reaches 3.36 W in pulsed mode at 20℃.This dual-mode laser chip has the potential for in-situ on-chip simultaneous detection of CH4and C2H6gases in leak monitoring. | XIYU LU YANJIAO GUAN PENGCHANG YANG SHAN NIU YU MA LIJUN WANG NING ZHUO JINCHUAN ZHANG SHENQIANG ZHAI FENGMIN CHENG SHUMAN LIU FENGQI LIU JUNQI LIU | 2023 | Photonics Research2023，11，12： | 0 |
| 13 | Technique of Earthworms Restoring Soil in Greenhouse Cultivation显示文摘The production environment of greenhouse cultivation is relatively closed,the multiple cropping index is high,the management of fertilizationwatering and pesticideapplication isblindtosomeextent,andthe phenomenonofcontinuous cropping isalsocommonSoilquali-ty affects the sustainable development of greenhouse cultivation.Earthworm is a ubiquitous invertebrate organism in soil,an important part of soil system,a link between terrestrial organisms and soil organisms,an important link in the small cycle of soil material organisms,and plays an important role in maintaining the structure and function of soil ecosystem.Different ecotypes of earthworms are closely related to their habi-tats(soil layers)and food resource preferences,and then affect their ecological functions.The principle of earthworm regulating soil function is essentially the close connection and interaction between earthworm and soil microorganism.Using different ecotypes of earthworms and bio-logical agents to carry out combined remediation of greenhouse cultivation soil is a technical model to realize sustainable development of green-house cultivation. | Yanjiao LI Jiafei SU Zhiu ZHANG Guang QI Jianhua CHEN Lixuan KOU Limin WANG Wenxian LIU Junyi ZHANG Libing QIU | 2024 | Asian Agricultural Research2024，16，1： | 0 |
| 14 | Phylogenetic Structure of Low Altitude Forest Communities in Baotianman Mountain显示文摘[Objectives]The paper was to reveal the phylogenetic structure of low altitude forest communities in Baotianman Mountain.[Methods]Ten 20 m×20 m plots were set at an altitude of 249-796 m in Baotianman Nature Reserve,in which 62 plant species were found.A phylogenetic tree was established on Phylomatic website,and the community related phylogenetic indexes at low altitude were calculated and analyzed,including net relatedness index(NRI),nearest taxon index(NTI),phylogenetic diversity Faith's PD index,phylogeneticβdiversity PhyloSor index and Dnn index.[Results]The NTI and NRI values of low altitude forest communities were generally greater than 0,showing phylogenetically clustered communities,and habitat filtration was the dominant factor in community construction.Combined with the species abundance matrix of plots,it was found that PD index had a significantly positive correlation with species richness.Phylogeneticβdiversity PhyloSor index was larger in the lower altitude area,which was exactly opposite to Dnn index.In other words,the more similar the community,the closer the distance between species,and the communities were phylogenetically clustered.[Conclusions]The study can provide a scientific basis for forest community management and restoration. | Jianghao ZHAO Yingying LIU Xiaoguo BAI Anping LI Yanjiao LI Shiping CHENG Guang QI | 2022 | Asian Agricultural Research2022，14，6： | 0 |
| 15 | The relative controls of temperature and soil moisture on the start of carbon flux phenology and net ecosystem production in two alpine meadows on the Qinghai-Tibetan Plateau显示文摘在温带和寒冷地区,春季植被物候的变化普遍受温度影响。然而,在寒冷地区的干旱/半干旱生态系统中,温度对物候的影响仍难以捉摸,因为土壤水分状况有调节物候的重要作用。利用生长度日(growing degree day,GDD)和生长季节指数(growing season index,GSI)模型,对最低温度(minimum daily temperature,T_(min))与土壤水分(daily soil moisture,SM)进行耦合,探索热量需求和水文气候交互作用对青藏高原(QTP)两个不同降水状况的高寒草甸生态系统碳吸收期开始日期(start of carbon uptake period,SCUP)和净生态系统初级生产力(net ecosystem productivity,NEP)的影响。其中,一种是水分限制的高寒草原草甸生态系统,另一种是温度限制的高寒灌丛草甸生态系统。GDD模型和GSI模型与SCUP明显相关联:在湿润的高寒灌丛草甸生态系统中,SCUP对生长季前GDD和GSI的变化同样敏感;而在半干旱高寒草原草甸生态系统中,与GDD相比,SCUP对生长季前GSI的变化更敏感。这些不同模式表明,限制SCUP的气候因子由温度和水分有效性的平衡来决定。在湿润的高寒草甸生态系统中,在不受干旱胁迫的情况下,较高的SCUP温度敏感性可以最大化热效益,正如观察到的SCUPs与GDD模型模拟具有较高的线性相关系数(R2)和AIC。而在半干旱的草原化草甸中,较高的SCUP水分敏感性可以最大化水分效益,正如观测到的SCUPs与GSI模型模拟具有较高的R2和AIC。此外,虽然在高寒灌丛草甸生态系统中SCUPs由GDD决定,但两个高寒草甸生态系统NEP均受累积GSI控制。本研究重点强调湿润和半干旱高寒草甸生态系统水文气候交互作用对春季碳通量物候和植被生产力的影响;揭示半干旱地区高寒草甸生态系统物候和碳平衡模型中应该包括温度和水分条件。这些结果对改善植被物候模型具有重要意义,从而加深我们对将来植被物候、生产力和气候变化相互作用的理解。 | Xi Chai Peili Shi Minghua Song Ning Zong Yongtao He Yingnian Li Xianzhou Zhang Yanjiao Liu | 2020 | Journal of Plant Ecology2020，13，2： | 0 |
| 16 | Effects of subtle change in side chains on the photovoltaic performance of small molecular donors for solar cells显示文摘Small-molecule organic solar cells(SMOSCs)have attracted considerable attention owing to the merits of small molecules,such as easy purification,well-defined chemical structure.To achieve high-performance SMOSCs,the rational design of well-matched donor and acceptor materials is extremely essential.In this work,two new small molecular donor materials with subtle change in the conjugated side thiophene rings are synthesized.The subtle change significantly affects the photovoltaic performance of molecular donors.Compared with chlorinated molecule MDJ-Cl,the non-chlorinated analogue MDJ exhibits decreased miscibility with the non-fullerene acceptor Y6,can more efficiently quench the excitons of Y6.As a result,a improved PCE of 11.16% is obtained for MDJ:Y6 based SMOSCs.The results highlight the importance of fine-tuning the molecular structure to achieve high-performance SMOSCs. | Xiang Gao Kuibao Yu Yanjiao Zhao Tao Zhang Jing Wen Zifeng Liu Zhihao Liu Guofeng Ye Jianhong Gao Ziyi Ge Zhitian Liu | 2022 | Chinese Chemical Letters2022，33，10： | 0 |
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