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| 1 | Experimental and clinic-opathologic study on the relationship between transcription factor Egr-1 and esophageal carcinoma显示文摘AIM To observe the growth suppression effect of exogenous introduction of early growth response gene-1 (Egr-1 gene) on esophageal carcinoma tissue as well as on esophageal carcinoma cell line Eca109 and to explore the potential application of Egr-1 gene in gene therapy of tumor.METHODS Eukaryotic expression vector of PCMV-Egr-1 plasmid was introduced into Eca109 cell line which expressed no Egr-1 protein originally with lipofectamine transfection method. The introduction and expression of PCMV-Egr-1 plasmid into Eca109 cell line was confirmed by G418 selection culture, PCR amplification of neogene contained in the vector, Western blot analysis and immunocytochemical analysis. The cell growth curve,soft agar colony formation rate and tumorigenicity in SClD mice were examined to demonstrate the growth suppression effect of exogenous Egr-1 gene on Eca109 cell line. The Egr-1 mRNA and Egr-1 protein were also detected in 50 surgical specimens of esophageal carcinoma by in situ hybridization and immunohistochemistry.RESULTS Exogenous Egr-1 gene was introduced successfully into Eca109 cell line and expressed Egr-1 protein stably. The transfected Eca109 cell line grew more slowly than control Eca109 as shown by cell growth curves, the soft agar colony formation rate (4.0% vs 6.9%, P<0.01) and the average growth rate of tumor in SCID mice (35.5 ± 7.6 vs 65.8 ± 7.6, P<0.05). The expression level of Egr-1 mRNA and protein significantly increased in dysplastic epithelia adjacent to cancer rather than in cancer tissues (65.8% vs 20.0% by ISH and 57.9% vs 14.0% by IHC, P<0.01).CONCLUSION Exogenous Egr-1 gene shows the strong effect of growth inhibition in Eca109 cell line. Egr-1 in the cancer tissue shows down-regulated expression that supports the inhibited function of Egr-1 in cancer growth and suggests Egr-1 may have an important role in gene therapy of esophageal carcinoma. | Ming-Yao Wu Mao-Huai Chen Ying-Rui Liang Guo-Zhao Meng Huan-Xing Yang Chu-Xiang Zhuang Department of Pathology,Shantou University Medical College,Shantou 515031,Guangdong Province,China Supported by the National Natural Science Foundation of China,No.39670298. | 2001 | World Journal of Gastroenterology2001，7，4： | 20 |
| 2 | Detection of the urban heat island in Beijing using HJ-1B satellite imagery显示文摘Satellite images are used extensively in studying the urban heat island(UHI) phenomenon.We evaluated the suitability of thermal infrared(TIR) data from the HJ-1B satellite for detecting UHI using a case study in Beijing.Two modified algorithms for retrieving the land surface temperature(LST) from HJ-1B data were tested.The results were compared with LST images derived from a Landsat TM thermal band and the MODIS LST output.The spatial pattern of UHI generated using HJ-1B data matched well with that produced using TM and MODIS data.Of the two algorithms,the mono-window algorithm performed better but further tests are necessary.With more frequent coverage than TM and higher spatial resolution than MODIS,the HJ-1B TIR data present a unique opportunity to study thermal environments in cities in China and neighboring countries. | YANG Jun1,GONG Peng2,ZHOU JinXing3,HUANG HuaBing2 & WANG Lei2 1 Key Laboratory of Silviculture and Conservation,Ministry of Education,Beijing Forestry University,Beijing 100083,China 2 State Key Laboratory of Remote Sensing Science,jointly sponsored by the Institute of Remote Sensing Applications,Chinese Academy of Sciences and Beijing Normal University,Beijing 100101,China 3 Institute of Desertification Studies,Chinese Academy of Forestry,Beijing 100091 China | 2010 | Science China Earth Sciences2010，53，S1： | 9 |
| 3 | Loose architecture of multi-level massive geospatial data based on virtual quadtree显示文摘This paper proposed a virtual quadtree (VQT) based loose architecture of multi-level massive geospatial data for integrating massive geospatial data dispersed in the departments of different hierarchies in the same sector into a unified GIS (Geographic Information System) platform. By virtualizing the nodes of the quad-tree,the VQT separates the structure of data organization from data storage,and screens the difference between the data storage in local computer and in the re-mote computers in network environment. And by mounting,VQT easily integrates the data from the remote computers into the local VQT so as to implement seam-less integration of distributed multi-level massive geospatial data. Based on that mode,the paper built an application system with geospatial data over 1200 GB distributed in 12 servers deployed in 12 cities. The experiment showed that all data can be seamlessly rapidly traveled and performed zooming in and zooming out smoothly. | YANG ChongJun,WU Sheng,REN YingChao,FU Li,ZHANG FuQing,WANG Gang,TAN Jian,LIU DongLin,MA ChaoJi & LIANG Li State Key Laboratory of Remote Sensing Science,Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University,Beijing 100101,China | 2008 | Science China(Technological Sciences)2008，51，S1： | 4 |
| 4 | Parallel algorithm for real-time contouring from grid DEM on modern GPUs显示文摘A real-time algorithm for constructing contour maps from grid DEM data is pre-sented.It runs completely within the programmable 3D visualization pipeline.The interpolation is paralleled by rasterizer units in the graphics card,and contour line extraction is paralleled by pixel shader.During each frame of the rendering,we first make an elevation gradient map out of original terrain vertex data,then figure out the final contour lines with image-space processing,and directly blend the results on the original scene to obtain a final scene with contour map using alpha-blending.We implement this method in our global 3D-digitalearth system with Direct3D?9.0c API and tested on some consumer level PC platforms.For arbitrary scene with certain LOD level,the process takes less than 10 ms,giving topologically correct,anti-aliased contour lines. | CHEN Zhuo,SHEN Lei,ZHAO YanQing & YANG ChongJun State Key Laboratory of Remote Sensing Science,Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal University,Beijing 100101,China | 2010 | Science China(Technological Sciences)2010，53，S1： | 3 |
| 5 | A Study of Feature Interactions in Intelligent Networks显示文摘 | YUAN Zhao rui directed by YANG Fang chun | 2002 | The Journal of China Universities of Posts and Telecommunications2002，9，1： | 3 |
| 6 | Rapid destruction of encapsulated islet xenografts by NOD mice is CD4-dependent and facilitated by B-cells: innate immunity and autoimmunity do not play significant roles 显示文摘 | Xu BY Yang H Serreze DV | 2005 | Transplantation2005，80，3： | 1 |
| 7 | Paeoniflorin, a potent natural compound, protects PC12 cells from MPP+ and acidic damage via autophagic pathway显示文摘 | Cao BY Yang YP Luo WF | 2010 | J Ethnopharmacol2010，131，1： | 1 |
| 8 | Protective Effect of Procyanidin B2 against CCl4- Induced AcutePLiver Injury in Mice显示文摘 | Yang BY Zhang XY | 2015 | Molecules2015，20，12： | 1 |
| 9 | Ultrastructural changes in rat locus coeruleus induced by chronic opioids显示文摘 | Miao H Qin By Yang Y | 1997 | Acta Neuropatho1997，94，： | 1 |
| 10 | The effects of tibial rotati on on the patellofemoral joint:assessment of the changes in situ strain in the peripatellav retina culum and the patellofemoral contalt pressunes and areas显示文摘 | Lee TQ Yang BY Sandusky MD | 2001 | J Rehabil Res Dev2001，38，5： | 1 |
| 11 | Dietary zinc intake is in-versely related to subclinical atherosclerosis measured by carotid intima-media thickness显示文摘 | Yang YJ Choi BY Chun BY | | 0，，08： | 1 |
| 12 | SynthesisofTi3AIC2 ceramic by high-energy ball milling of elemental powders of Ti, AI and C 显示文摘 | YANG C JI N SZ LI ANG BY etal | 2009 | Journal of Materials Process and Technology2009，209，2： | 1 |
| 13 | Bone mineral density in women receiving thy- roxine suppressive therapy for diferenti- ated thyroid carcinoina显示文摘 | Chen CH Chen JF Yang BY | 2004 | J Formos Meal Assoc2004，1030，： | 1 |
| 14 | 显示文摘 | Haisong Qi Quanling Yang Lina Zhang eteThe dissolution of ceUu- lose in NaOH - based aqueous system by two - step process | 2011 | Cel- lulose2011，18，2： | 1 |
| 15 | Cyclosporin A inhibits the production of IL-17 by memory Thl7 cells from healthy individuals and patients with rheumatoid arthritis 显示文摘 | Zhang C Zhang JY Yang BY | 2008 | Cytokine2008，42，3： | 1 |
| 16 | Simultaneous determination of four components in composite vitamin B tablets using a square -root kalman filter显示文摘 | Yang JH Han RJ Su BY | 1998 | Analytical Science1998，14，5： | 1 |
| 17 | Role of rosiglitazone in lipopolysaccharide-indueed peritonitis: a rat peritoneal dialysis model显示文摘 | Song SH Kwak IS Yang BY | 2009 | Nephrology (Carlton)2009，14，2： | 1 |
| 18 | Proteomic analysis reveals ATP-dependent steps and chaperones involvement in luteolin-induced lung cancer CH27 cell apoptosis显示文摘 | Lee HZ Yang WH Bao BY | 2010 | Eur J Pharmacol2010，642，123： | 1 |
| 19 | Photodynamic activiyy of aloe-emodin induces resensitization of lung cancer cells to anoikis 显示文摘 | Lee HZ Yang WH Bao BY | 2010 | Eur J Pharmacol2010，648，1： | 1 |
| 20 | New antiproliferatlve and immuno- suppressive withanolides from the seeds of Datura metel 显示文摘 | Yang BY Xia YG Liu Y | 2014 | Phyto- chemistry Letters2014，，8： | 1 |
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