| 1 | Ribosome Biogenesis Factor Bmsl-like Is Essential for Liver Development in Zebrafish显示文摘Ribosome biogenesis in the nucleolus requires numerous nucleolar proteins and small non-coding RNAs.Among them is ribosome biogenesis factor Bmsl,which is highly conserved from yeast to human.In yeast,Bmsl initiates ribosome biogenesis through recruiting Rcll to pre-ribosomes.However,little is known about the biological function of Bmsl in vertebrates.Here we report that Bmsl plays an essential role in zebrafish liver development.We identified a zebrafish bms1l^(sq163) mutant which carries a T to A mutation in the gene bmsl-like(bms1l).This mutation results in L^(152) to Q^(152) substitution in a GTPase motif in Bmsll.Surprisingly,bmsll^(sq163) mutation confers hypoplasia specifically in the liver,exocrine pancreas and intestine after 3 days post-fertilization(dpf).Consistent with the bmsll^(sq163) mutant phenotypes,whole-mount in situ hybridization(WISH) on wild type embryos showed that bmsll transcripts are abundant in the entire digestive tract and its accessory organs.Immunostaining for phospho-Histone 3(P-H3) and TUNEL assay revealed that impairment of hepatoblast proliferation rather than cell apoptosis is one of the consequences of bms1l^(sq163) giving rise to an under-developed liver.Therefore,our findings demonstrate that Bmsll is necessary for zebrafish liver development. | Yong Wanga,Yue Luoa,Yunhan Hong(b,),Jinrong Peng(a,),Lijan Lo(a,b,) a College of Animal Sciences,Zhejiang University,Hangzhou 310058,China b Department of Biological Sciences,National University of Singapore,Science Drive 4,Singapore 117543,Singapore | 2012 | Journal of Genetics and Genomics2012，39，9： | 4 |
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