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| 1 | CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant显示文摘Coronavirus disease 2019(COVID-19)is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expensive instrumentation,and the confirmation of variants relies on further sequencing techniques.Herein,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 variant.To enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold nanoparticles.Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays.The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system.Furthermore,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing. | Chenshuo Wu Zhi Chen Chaozhou Li Yabin Hao Yuxuan Tang Yuxuan Yuan Luxiao Chai Taojian Fan Jiangtian Yu Xiaopeng Ma Omar AAl-Hartomy SWageh Abdullah GAl-Sehemi Zhiguang Luo Yaqing He Jingfeng Li Zhongjian Xie Han Zhang | 2022 | Nano-Micro Letters2022，14，10： | 2 |
| 2 | Single crystal growth, magnetic properties and Schottky anomaly of HoFeO 3 orthoferrite显示文摘 | Mingjie Shao Shixun Cao Yabin Wang Shujuan Yuan Baojuan Kang Jincang Zhang Anhua Wu Jun Xu | 2010 | Journal of Crystal Growth2010，，1： | 1 |
| 3 | Large magnetocaloric effect in HoFeO 3 single crystal显示文摘 | Mingjie Shao Shixun Cao Yabin Wang Shujuan Yuan Baojuan Kang Jincang Zhang | 2012 | Solid State Communications2012，，11： | 1 |
| 4 | Effect of super-chilling storage on maintenance of quality and freshness of the Pacific oyster(Crassostrea gigas)显示文摘The quality changes of shelled Pacific oysters(Crassostrea gigas)were examined in relation to the effects of superchilling storage at-1 C for 28 d by measuring changes in biochemical properties(microbial analysis,adenosine triphosphate(ATP)-related compounds,pH,free amino acids)and sensory evaluations in this study.The results indicated that microorganism growth was significantly inhibited during superchilling storage.Adenosine diphosphate(ADP)and adenosine monophosphate(AMP)accumulated while ATP rapidly decreased in the adductor muscle.ATP and ADP were the primary components in the other 3 tissues including mantle,gill,and body trunk of oysters,and they remained relatively stable over time.The pH and adenylate energy charge in the adductor muscle could be utilized as freshness indicators for shelled oysters.However,there were no significant differences(P>0.05)among the free amino acids during whole storage.According to the sensory evalu-ations,oysters could be alive and tolerated up to 21 d at-1℃storage.The study demonstrated that superchilling storage at-1℃could better maintain the eating quality of shelled oysters and the shelf life was extended to 21 d. | Shiliang Dong Yabin Niu Huamao Wei Yumeng Lin Xin Lu Tetsuro Yamashita Kefeng Yu Koichi Takaki Chunhong Yuan | 2023 | Food Quality and Safety2023，7，2： | 0 |
| 5 | Screening and Insert Size Analysis of Candidate C-Genome BAC Clones from Brassica napus显示文摘Thirty-two C-genome specific candidate bacterial artificial chromosome (BAC) clones were successfully screened from the BAC library by four-dimensional PCR method with the primer pairs of 75 simple sequence repeat (SSR) markers located in the nine C-genome linkage groups of Brassica napus. The screened 32 BAC clones have an average insert size of 114.2 kb with a range of 30-190 kb. They are the first set of C-genome BAC clones screened from B. napus genomic BAC library. The average insert size of this set of BAC clones presented that the constructed BAC library had a high quality. This set of BAC clones can be used as markers to identify individual chromosomes of B. napus C-genome. | YAN Xiaohong YUAN Yabin ZHAO Xueya ZHA Minmin DAI Shuang LIU Feng WANG Yuantao WEI Wenhui | 2012 | Wuhan University Journal of Natural Sciences2012，17，4： | 0 |
| 6 | Dynamic Changes of China’s Functional Specialization in Export and International Comparison under Global Value Chains显示文摘The functional specialization in export of a country(and its sectors)in different activities,such as fabrication,R&D,management and marketing,is crucial to its governance and control on the value chains,which magnifies the shortage of the existing aggregate value added studies on our understanding of global value chains(GVCs).Considering production fragmentation at both the spatial and functional levels,this paper defines the modified functional specialization indicators at the national and sectoral levels from the forward linkage(rather than backward linkage).Based on the World Input-Output Database together with the newly compiled Labor Occupations Database,this paper re-estimates and analyzes the functional specialization and changes in China and major developed economies’exports.The results show that China’s export is mainly specialized in fabrication activity,which is among the world leading level,while it is weak in headquarter activities(especially R&D and management),which is almost locked at the lowest level in the world and could not pose an export threat to the developed economies.China’s manufacturing basically follows the functional development path of“relying on fabrication,entering market,targeting management and R&D”,featuring the coexistence of“strong”fabrication and“weak”management and R&D.The fabrication specialization of the typical processing sector“electronic and optical equipment”has reached the international leading level.The level of functional specialization of China’s service industry is generally lower than that of manufacturing and generally lags behind in the world,indicating that China still has a long way to go before becoming a major power of service in the world.Finally,this paper proposes policy implications and further researches that can be extended. | Zhenguo Wang Yabin Zhang Meng Niu Yuan Zhong | 2020 | China Finance and Economic Review2020，9，3： | 0 |
| 7 | Effects of flash-boil shucking on biochemical,sensorial and ultrastructural properties of Yesso Scallop(Mizuhopecten yessoensis)adductor muscle during refrigeration显示文摘Scallop adductor muscle has great popularity for raw consumption in Japan,but conventionally shucking live scallops with high vitality by knife requires skill and is time-consuming.Flash-boil shucking is a convenient shucking method in sushi restaurants for live scallops.However,the effects of this treatment on the quality of its main edible part,the adductor muscle,are not clear.This study was conducted to investigate the impact of flash-boil shucking on the quality of raw-edible scallop adductor muscle,focusing on flavor-enhancing components and ultrastructure.Thermal treatment could contribute to the formation of flavor-enhancing nucleotides in the external layer of the adductor muscle.Adenosine monophosphate content increased with the boiling time,which significantly increased to 1.47±0.42μmol/g in the 30 s-boiled group compared with 0.38±0.13μmol/g in the control group(p<0.05).The rapid adenosine monophosphate accumulation after boiling resulted in a faster accumulation of inosine and hypoxanthine during subsequent refrigeration,which could bring bitterness to aquatic products.Flash-boil shucking had no discernible effect on the proportion of free amino acids relevant to the flavor of scallop adductor muscle,despite the possibility of a reduction in total free amino acids.The external layer of the scallop adductor muscle could be hardened after flash-boil shucking.At the ultrastructural level,muscle fibers tend to aggregate transversely and contract longitudinally depending on the heating time.Flash-boil shucking is ideal for consuming the raw-edible scallop adductor muscle promptly.Flash-boil shucking treatment is recommended for households,restaurants,and small factories for treating scallops with high vitality. | Yabin Niu Shiliang Dong Nobuyoshi Wada Huamao Wei Tetsuro Yamashita Koichi Takaki Chunhong Yuan | 2023 | Food Quality and Safety2023，7，3： | 0 |
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