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| 1 | Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector显示文摘AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral vector pZIP cDNA.METHODS Human IL-6 gene was reconstructedin retrovirus vector and transfected into incasingcells PA317 by lipofectamine mediated method,the clones of the cells transferred with hlL-6were selected by G418,and targeted HT-29 cellswere infected with the virus granules secretedfrom PA317 and also selected by G418.Test genetranscription and expression level byhybridization,ELISA and MTT assay,etc.Analyze tumor inhibitory effects according to thecell growth curve,plating forming rate andtumorigenicity in nude mice.RESULT Successfully constructed andtransfected recombinant expressing vectorspZIPIL-6 cDNA and got positive transfected celllines.The colon cancer cell line(HT-29 IL-5)transfected with the hlL-5 gene by retroviralvector was established.The log proliferationperiod and the doubling time of this cell line wasbetween 4 to 7 days and 2.5 days according tothe direct cell count,the cell proliferation wasobviously inhibited with MTT assay,the platinginhibitory rate was 50% by plating efficiencytest.When HT-29 IL-6 cells were inoculated intothe nude mice subcutaneously,carcinogenicactivity of the solid tumor was found superior tothe control group and the size of tumor was notsignificantly enlarged.Injection of combinationvirus fluid containing 11.-6 gene intotransplantation tumors could inhibit the growthand development of the tumor.CONCLUSION IL-6 could inhibit the growth andproliferation of colon cancer cells by retroviralvector-mediated transduction. | Xiao B Jing B Zhang YL Zhou DY Zhang WD | 2000 | World Journal of Gastroenterology2000，6，1： | 11 |
| 2 | Laparoscopic hepatectomy by curettage and aspiration:a new technique显示文摘背景 Laparoscopic 外科为最小的侵略和快速的手术后的恢复是有益的。自从在在 1990 年代的肝切除术的 laparoscopic hepatectomy 的使用,它在有效、安全的技术的一些机构 worldwide.Lack 被执行了因为肝办理是在 1998 由刮术和渴望为 laparoscopic hepatectomy 阻止一种新技术它开发的进一步的 development.We 的主要障碍。在这份报纸,我们总共在 7 年 practice.Methods 以后分析这种技术的临床的结果 59 个连续病人在我们的机构从 1998 年 8 月由刮术和渴望经历了 laparoscopic hepatectomy 到 2005 年 1 月。这些病人包括了 33 男性和 26 女性,与 47 年的吝啬的年龄。为肝 transecsion laparoscopic, Peng 的多功能的起作用的析象器(LPMOD ) 被使用。损害在 5 .Procedures 在 19 个病人,在 17 的良性的肝肿瘤,在 18 的 intrahepatic 演算,和另外的肝损害包括了恶意的肝肿瘤在 30 个病人,的包括的本地切除术离开了在 28 的侧面的 segmentectomy,和权利在 1 个 .Results Laparoscopic 手术的 hemihepatectomy 在 57 个病人被完成。二个病人(3.4%) 把操作变换成剖腹术。吝啬的操作时间是 143 分钟,吝啬的 intraoperative 血损失是 456 mi。手术后的医院停留的吝啬的长度是 7 天。复杂并发症在 2 个病人(3.4%) 发生了,并且由刮术没有 perioperative death.Conclusion Laparoscopic hepatectomy,渴望为肝切除术有效、安全。 | CAI Xiu-jun LIANG Xiao WANG Yi-fan YU Hong ZHENG Xue-yong HUANG Di-yu PENG Shu-you Department of General Surgery,Sir Run Run Shaw Hospital,College of Medicine Institute of Minimally Invasive Surgery of Zhejiang University,Hangzhou 310016,China (Cai XJ,Liang X,Wang YF,Yu H,Zheng XY,Huang DY and Peng SY) | 2007 | Chinese Medical Journal2007，，20： | 11 |
| 3 | Experimental study on the phase change behavior of phase change material confined in pores显示文摘 | Zhang D Tian SL Xiao DY | 2007 | Solar Energy2007，81，5： | 1 |
| 4 | Studying cardiac contractility change trend to evaluate cardiac reserve显示文摘 | Xiao SZ Wang ZG Hu DY | 2002 | IEEE Engineering in Medicine and Biology2002，21，1： | 1 |
| 5 | Clinical application of intraperitoneal chemotherapy before surgery for ovarian cancer and its evaluation显示文摘 | Shi DY Xiao M Chen SY | | 0，，： | 1 |
| 6 | The Asian nitrogen cycle case study 显示文摘 | Zheng X Fu C Xu X Xiao DY Huang Y Chen G Han S Hu F | 2002 | Ambio2002，31，： | 1 |
| 7 | Draft genome of the kiwifruit Actinidia chinensis 显示文摘 | Huang SX Ding J Deng DJ Tang W Sun HH Liu DY Zhang L Niu XL Zhang X Meng M Yu JD Liu J Han Y Shi W Zhang DF Cao SQ Wei ZJ Cui YL Xia YH Zeng HP Bao K Lin L Min Y Zhang H Miao M Tang XF Zhu YZ Sui Y Li GW Sun HJ Yue JY Sun JQ Liu FF Zhou LQ Lei L Zheng XQ Liu M Huang L Song J Xu CH Li JW Ye KY Zhong SL Lu BR He GH Xiao FM Wang HL Zheng HK Fei ZJ Liu YS | 2013 | Nat Commun2013，4，： | 1 |
| 8 | Experimental study on the phase change behavior of phase change material confined in pores显示文摘 | Zhang D Tian SL Xiao DY | 2007 | Solar Energy2007，81，5： | 1 |
| 9 | Physiological response to pressure breathing witha capstan counter pressure vest显示文摘 | Xiao H J He DY | 1992 | SAFE Journal1992，，： | 1 |
| 10 | Studying cardiac contractility change trend to evaluate cardiac reserve 显示文摘 | Xiao SZ Wang ZG Hu DY | 2002 | IEEE Engineering in Medicine and Biology2002，21，1： | 1 |
| 11 | Development of optimal techniques for cryopreservation of human platelets显示文摘 | Gao DY Neff K Xiao HY | 1999 | Cryobiology1999，38，： | 1 |
| 12 | Comparative study on short - term and long - term prognostic determinants in patients with acute cerebral infarction 显示文摘 | Jie W Xiao DY Guang QL | 2015 | Int J Clin Exp Med2015，8，6： | 1 |
| 13 | Studying cardiac contractility change trend to evaluate cardiac reserve 显示文摘 | Xiao SZ Wang ZG Hu DY | 2002 | IEEE Eng Med Biol2002，21，1： | 1 |
| 14 | Development of optimal techniques for cryopreversation of human platelets显示文摘 | Gao DY Neff K Xiao HY | 1999 | Cryobiology1999，38，1： | 1 |
| 15 | Diagnostic Significance of intracystic nodules on MRI in Rathke's cleft cyst 显示文摘 | Wang SS Xiao DY Yu YH | 2012 | Int J Endocrinol2012，2012，95： | 1 |
| 16 | Effect of minor cerium additions on microstructure and mechanical properties of cast Al-Cu-Mg-Ag alloy显示文摘 | Xiao DH Wang JN Ding DY | | 0，，： | 1 |
| 17 | Development of optimal techniques for cryopreservation of human platelets显示文摘 | Gao DY Neff K Xiao HY | 1999 | Cryobiology1999，38，： | 1 |
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