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您的检索式：作者名="XIE Guangxin"
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| 1 | Secondary biogenic coalbed gas in some coal fields of China显示文摘The secondary biogenic coalbed gas, a new genetic and energy source type of coalbed gas in China, has been found in Xinji, Liyazhuang and Enhong areas. The essential characteristics of this type of gas are: (i) the major component of the gas is methane, with C1/C1-5 value higher than 0.99, indicating that the gas is part of dry gas; (ii) theδ13C1 value is in the range of -61.7‰to -47.9‰, mostly lower than -55‰, which is much lower than the estimatedδ13C1 value of thermogenic methane according to the thermal evolution degree of the coal rocks (with R0 value from 0.87% to 1.43%), showing the characteristics of the secondary biogenic gas; (iii) theδ5D value of methane ranges from -244‰to -196‰; (iv)δ13C 2 value ranges from -26.7‰to -15.9‰andδ13C 3 value ranges from -10.8‰to -25.3‰, indicating that the heavier hydrocarbons have a thermogenic origin; (v) the content of CO2 is very low, andδ13CCO2 value changes greatly, reflecting a characteristic of secondary change; (vi)δ15N2 value ranges mainly from -1‰to +1‰, indicating N2 derived significantly from air. The negative linear correlation between the contents of N2 and CH4 reflects the activity of bacteria bearing surface water infiltrating into coal beds. The comprehensive tracing indices show that the coalbed gas in the studied areas is the mixed gas of primarily secondary biogenic gas and a part of remnant thermogenic gas. The uplift of coal beds and the development of faults in the studied areas create favorable conditions for the formation of the secondary biogenic gas. | TAO Mingxin WANG Wanchun XIE Guangxin LI Jinying WANG Yanlong ZHANG Xiaojun ZHANG Hong SHI Baoguang GAO Bo | 2005 | Chinese Science Bulletin2005，50，S1： | 8 |
| 2 | Identification,characterization and expression analysis of rLcn13,an epididymal lipocalin in rats显示文摘As the essential tissue for sperm maturation and storage,the epididymis secretes a number of tissue-specific proteins to exert its functions.Among these proteins,epididymal lipocalins have been intensively studied because of their epididymis-specific expression pattern and clustered genomic organization.In this study,rLcn13,a member of the rat epididymal lipocalin family,is identified and elaborately characterized.The cDNA sequence of rLcn13 consists of 719 nucleotides and encodes a 176 amino-acid protein with a predicted N-terminal signal peptide of 19 amino acids.rLcn13 shares a similar genomic structure and predicted 3D protein structure with other lipocalin family members.A recombinant rLCN13 mature peptide of 157 amino acids is expressed and purified,which is used to raise a polyclonal antibody against rLCN13 with high specificity and sensitivity.Northern blot,western blot,and immunohistochemistry assays reveal that rLcn13 is an epididymis-specific gene which is expressed predominantly in the initial segment and proximal caput epididymis and influenced by androgen.The rLCN13 protein is modified by N-glycosylation and secreted into the epididymal lumen,and then binds to the acrosome region of the sperm.Our data demonstrate that rLcn13 exhibits a specific temporospatial expression pattern and androgen dependence,indicating its potential roles in sperm maturation. | Guangxin Yao Shengsong Xie Xiaofeng Wan Ling Zhang Qiang Liu Shuanggang Hu | 2023 | Acta Biochimica et Biophysica Sinica2023，55，2： | 0 |
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