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| 1 | Magnetic resonance evaluation of transplanted mesenchymal stem cells after myocardial infarction in swine显示文摘Objectives To trace and evaluate intracoronary transplanted mesenchymal stem cells(MSCs) labeled with superparamagnetic iron oxide(SPIO) by using magnetic resonance imaging(MRI) in a swine model of myocardial infarction (MI).Methods MSCs were transfected with a lentiviral vector carrying the gene encoding green fluorescent protein (GFP) and labeled in vitro with SPIO.Two weeks after MI, swine were randomized to intracoronary transplantation of dual -labeled MSCs(n = 10),MSCs-GFP(n = 10) and saline(n = 5).MRI examination was performed with a 1.5T clinical scanner at 24 hours,3 weeks and 8 weeks after cells transplantation. Signal intensity(SI) changes,cardiac function and MI size were measured using MRI.Correlation between MR findings and histomorphologic findings was also investigated. Results The labeling efficiency at a combination of 25μg Fe/ml SPIO and 0.8 pi/ml Lipofectamine 2000 reached 100%.SPIO labeling did not affect GFP fluorescence and dual-labeling did not affect cell proliferation(P>0.05). Multipotentiality was not affected especially for cardiomyocyte-like cells differentiation.Cardiac cell marker of a-MHC and actinin were positively expressed by immunofluorescence staining after induction.SI on T2 * WI decreased substantial- ly in the interventricular septum 24 hours after injection of MSCs.The intensity of hypo-intense signals appeared to increase throughout the later time points.Changes in SI at 24 hours,3 weeks and 8 weeks were 52.98%±10.74%,21.53%±5.40%and 6.23%±2.01%,respectively(P<0.01).DE-MRI demonstrated both dual-labeled MSCSs and MSCs-GFP could dramatically reduce the size of MI and improve cardiac function. Histological data revealed that prussian blue stain-positive cells were found mainly in the border zone which also showed green fluorescence but negative for macrophage marker(CD68).Gross pathologic examination revealed that engrafted MSCs dramatically reduce the extent of necrotic myocardium and promote the regeneration of new,contractile myocardium along the subendocardial surface of the MI. Conclusions MSCs could be efficiently and safely labeled with SPIO and GFP,and could be detected reproducibly and noninvasively in vivo using cardiac MRI.Intracoronary transplantation of dual-labeled MSCs could increase cardiac function and reduce the size of MI. | KE Yang~1,PENG Chao-quan~1,XIANG Peng~1,ZHANG Cheng-xi~1, ZOU Li-yuan~1,WU Xiao~1,YA Gao~1,KANG Z huang~2,HE Ke-ke~2,LIU Jin-lai~1 (1.Department of Cardiology,The Third Affiliated Hospital, Sun Yat-sen University,Guangzhou 2.Department of Radiology, the Third Affiliated Hospital,Sun Yat-sen University, Guangzhou 5100630 3.Center for Stem Cell Biology and Tissue Engineering,Sun Yat-sen University,Guangzhou 5100120) | 2011 | 岭南心血管病杂志2011，17，S1： | 7 |
| 2 | Size effect in compression fracture: splitting crack band propagation显示文摘 | Bazant Z P Xiang Y Y | 1997 | Journal of Engineering Mechanics American Society of Civil Engineering1997，123，2： | 2 |
| 3 | Regression of engineered myeloma cells secreting interferon-gamma-inducing factor is mediated by both CD4(+)/CD8(+)T and natural killer cells显示文摘 | Xiang J Chen Z Huang H | 2001 | Leuk Res2001，25，10： | 1 |
| 4 | An effective wavelet matrix transform approach for efficient solutions of electromagnetic integral equations显示文摘 | XIANG Z LU Y | 1997 | IEEE Trans AP1997，45，8： | 1 |
| 5 | 显示文摘 | Xiang L Xiang Y Wang Z G | 2002 | Powder Technol2002，126，： | 1 |
| 6 | Formation and Magnetic Properties of M-Sr Ferrite Hollow Fibers Via Organic Gel-precursor Transformation Process显示文摘 | SONG F Z SHEN X Q XIANG J | 2010 | Mate- rials Chemistry and Physics2010，120，： | 1 |
| 7 | Slurry phase Fischer-Tropsch synthesis over manganese-promoted iron ultrafine particle catalyst显示文摘 | BAI L XIANG H W LI Y W HAN Y Z ZHONG B | 2002 | Fuel2002，81，1112： | 1 |
| 8 | On the existence of 2n-periodic solutions of duffing type equation x''( t ) + g (x (t - r) = p (t))显示文摘 | HUANG X K XIANG Z G | 1994 | Chinese Science Bulletin1994，39，3： | 1 |
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| 10 | Effects of ECAE process on microstructure and transformation behavior of TiNi shape memory alloy 显示文摘 | LI Z H XIANG C Q CHENG X H | 2006 | Materials and Design2006，27，4： | 1 |
| 11 | Crocetin inhibits leu?kocyte adherence to vascular endothelial cells induced by AGEs显示文摘 | XIANG M QIAN Z Y ZHOU C H | 2006 | J Ethnopharmacol2006，107，1： | 1 |
| 12 | Dexmedetomidine Controls Systemic Cytokine Levels through the Cholinergic Anti-inflammatory Pathway显示文摘 | Xiang H Hu B Li Z | 2014 | Inflammation2014，37，5： | 1 |
| 13 | Optimal crashworthiness design of a spot-welded thin-walled hat section显示文摘 | Xiang Y J Wang Q Fan Z J | 2006 | Finite Element in Analysis and Design2006，，42： | 1 |
| 14 | Role of Wnt/β- catenin sig- naling in epithelial differentiation of lung resident mesenchy- mal stem cells 显示文摘 | SHI C LV T XIANG Z | 2015 | J Cell Biochem2015，116，8： | 1 |
| 15 | Dexmedetomidine controlssystemic cy- tokine levels through the cholinergic anti-inflammatorypathway 显示文摘 | Xiang H Hu B Li Z | 2014 | Inflammation2014，37，5： | 1 |
| 16 | AMPK, the metabolic syndrome and cancer显示文摘 | Luo Z Saha AK Xiang X | 2005 | Trends Pharmacol Sci2005，26，2： | 1 |
| 17 | Color Fine-Tuning of CNTs@AAO Composite Thin Films via Isotropically Etching Porous AAO Before CNT Growth and Color Modification by Water Infusion显示文摘 | Xiang L Z Guowen M | 2010 | Adv Mater2010，22，： | 1 |
| 18 | Peer-to-peer based muhimedia distribution service显示文摘 | Xiang Z Zhang Q Zhu W | 2004 | IEEE Transaction on Muhimedia2004，6，4： | 1 |
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| 20 | Insulin degrad- ing enzyme activity selectively decreases in the hi- ppocampal formation of cases at high risk to de- velop Alzheimer's disease显示文摘 | Zhao Z Xiang Z Haroutunian V | 2007 | Neurobiol Aging2007，28，6： | 1 |
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