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| 1 | 雪貂、猫、犬及其它家畜禽对SARS-CoV-2易感性的研究显示文摘哺乳动物及禽类中一直有不同的冠状病毒流行,但这些冠状病毒大多仅限于感染特定种属动物,一般不能感染人类。同样,人常见的几种冠状病毒也不感染其它动物。但是,来自蝙蝠的某些冠状病毒,如SARS和中东呼吸综合征病毒(MERS),往往可以感染多种动物和人类。SARS-CoV-2目前在全球大范围的人群中流行,传入与人类密切接触的动物的风险与日俱增。因此,迫切需要开展与人密切接触的家养动物对SARS-CoV-2的易感性研究,评估家养动物是否面临SARS-CoV-2危害并成为潜在中间宿主的风险,为防控SARS-CoV-2引起的疾病提供科学依据。鉴于目前COVID-19在全球的流行情况,其演变为常在传染病的可能性很大,急需建立稳定、可靠的动物感染模型,满足疫苗和药物研发的评价需求。 | SHI JIAN-ZHONG WEN ZHI-YUAN ZHONG GONG-XUN | 2020 | 中国预防兽医学报2020，42，9： | 56 |
| 2 | Wenchuan earthquake:Brightness temperature changes from satellite infrared information显示文摘By using an alternative processing method for satellite infrared remote sensing data and adopting the China Geostationary Meteorological Satellite(FY-2C) infrared remote sensing brightness temperature data,we studied the great 2008 Wenchuan Ms8.0 earthquake.Results indicate the obvious characteristic period and amplitude,and distribution of thermal infrared anomalies before the Wenchuan earthquake.And we discussed the mechanism of the observed pre-earthquake thermal anomalies.The characteristics of observed thermal infrared anomalies are easy to be recognized and applied,and could provide a criterion for thermal anomalies of earthquakes. | ZHANG YuanSheng GUO Xiao ZHONG MeiJiao SHEN WenRong LI Wen HE Bin | 2010 | Chinese Science Bulletin2010，55，18： | 47 |
| 3 | Effect of Helicobacter pylori infection on gastric epithelial proliferation in progression from normal mucosa to gastric carcinoma显示文摘EfectofHelicobacterpyloriinfectionongastricepithelialproliferationinprogressionfromnormalmucosatogastriccarcinomaLIUWenZhon... | LIU Wen Zhong, ZHENG Xiong, SHI Yao, DONG Quan Jiang and XIAO Shu Dong | 1998 | World Journal of Gastroenterology1998，4，3： | 30 |
| 4 | U-Pb zircon geochronology and Hf isotope study of metamorphosed basic-ultrabasic rocks from metamorphic basement in southwestern Zhejiang:The response of the Cathaysia Block to Indosinian orogenic event显示文摘A combined study using LA-ICP-MS U-Pb dating, Hf isotopes, trace elements and the Ti-in-zircon geo-thermometer was carried out on zircons from the metamorphosed basic-ultrabasic rocks in the meta-morphic basement of the Cathaysia Block, southwestern Zhejiang Province. The formation and meta-morphic ages of the rocks from the metamorphic basement of the Cathaysia Block were determined based on zircon U-Pb geochronology. The age for the magmatic crystalline zircons from the protolith is about 1.85 Ga. The εHf(t) values of the older zircons were from ?7 to ?3, with two-stage model Hf ages (TDM2LC) of about 2.9 to 3.4 Ga, indicating that the source material was derived from anatexis and recy-cling of the Archean crust. The newly formed metamorphic zircons yielded U-Pb ages of 260―230 Ma. The metamorphic temperature calculated using the Ti-in-zircon geothermometer ranged from 610 to 720℃, consistent with the results from petrographic observations, indicating that the Cathaysia Block experienced an amphibolite facies metamorphism during the Indosinian. Results from this study pro-vided an important timeframe for the tectonic evolution in South China and the Southeast Asia during the Late Permian and Early Triassic times. | XIANG Hua ZHANG Li ZHOU HanWen ZHONG ZengQiu ZENG Wen LIU Rui JIN Song | 2008 | Science China Earth Sciences2008，51，6： | 24 |
| 5 | Paleoproterozoic reworking of ancient crust in the Cathaysia Block,South China:Evidence from zircon trace elements,U-Pb and Lu-Hf isotopes显示文摘A combined study of zircon LA-ICP-MS U-Pb dating, trace elements and Hf isotope was carried out for gneissic granite from the Sanzhishu area in Jingning, SW Zhejiang Province. Nearly all the zircons separated from the granite exhibited oscillatory zoning and high Th/U ratios (>0.1). The REE profile showed a pronounced positive Ce anomaly, negative Eu anomaly and an enrichment of HREE, which are typical characteristics of magmatic zircon. Thirteen concordant or nearly concordant analytical data yielded a weighted mean 207Pb/206Pb age of 1860±13 Ma (MSWD=0.084), representing the formation age of the granite. The magmatic zircons had negative εHf(t) values of -15.6 to -10.0 and two-stage Hf model ages of 3.1 to 3.4 Ga, indicating that the granites were formed by reworking of ancient crust. The major- and trace-element data indicate that the gneissic granites are metaluminous high-K calc-alkaline rocks and exhibit the same geochemical characteristics as aluminous A-type granites, implying the emplacement of the granite in a post-orogenic extensional tectonic setting. We conclude that the Pa-leoproterozoic crustal reworking event in the Cathaysia Block of South China marked the transition from assembly to break-up of the Columbia supercontinent. | LIU Rui ZHOU Han Wen ZHANG Li ZHONG ZengQiu ZENG Wen XIANG Hua JIN Song LU Xin Qian LI ChunZhong | 2009 | Chinese Science Bulletin2009，54，9： | 22 |
| 6 | 3D bioprinting for biomedical devices and tissue engineering: A review of recent trends and advances显示文摘3D printing,an additive manufacturing based technology for precise 3D construction,is currently widely employed to enhance applicability and function of cell laden scaffolds.Research on novel compatible biomaterials for bioprinting exhibiting fast crosslinking properties is an essential prerequisite toward advancing 3D printing applications in tissue engineering.Printability to improve fabrication process and cell encapsulation are two of the main factors to be considered in development of 3D bioprinting.Other important factors include but are not limited to printing fidelity,stability,crosslinking time,biocompatibility,cell encapsulation and proliferation,shear-thinning properties,and mechanical properties such as mechanical strength and elasticity.In this review,we recite recent promising advances in bioink development as well as bioprinting methods.Also,an effort has been made to include studies with diverse types of crosslinking methods such as photo,chemical and ultraviolet(UV).We also propose the challenges and future outlook of 3D bioprinting application in medical sciences and discuss the high performance bioinks. | Soroosh Derakhshanfar Rene Mbeleck Kaige Xu Xingying Zhang Wen Zhong Malcolm Xing | 2018 | Bioactive Materials2018，3，2： | 21 |
| 7 | Caledonian reworking of Paleoproterozoic basement in the Cathaysia Block: Constraints from zircon U-Pb dating, Hf isotopes and trace elements显示文摘A combined study of zircon U-Pb dating, Hf isotopes and trace elements has been carried out for granodioritic neosomes of migmatites from the Tianjingping area in northwestern Fujian Province. Zircons are characterized by zoning, higher Th/U ratios (mostly ≥0.1), HREE enrichment, and positive Ce and negative Eu anomalies, and show features similar to magmatic or anatectic zircons. Apparent 206Pb/238U ages for the zircons are 447±2 Ma (95% conf., MSWD = 0.88), corresponding to a Caledonian event. εHf(t) values are ?13.3 to ?9.7, indicating a crustal source. Two-stage Hf model ages are 1.7 to 1.9 Ga, suggesting that protolith of the migmates was probably formed in the Paleoproterozoic. The granodioritic neosomes have the characteristics of peraluminous calc-alkaline granite, and their REE patterns and trace elements spidergrams show features of middle to upper crustal rocks. Together with previous studies, we conclude that the protolith of the Cathaysia basement in the Tianjingping area was likely formed in the middle-late Paleoproterozoic and experienced partial melting during the Caledonian period. The recognition of Caledonian reworking of the Paleoproterozoic basement in the Cathaysia Block provides a new insight into the tectonic evolution of the Cathaysia Block in the Caledonian pe- riod and the interaction between the Cathaysia Block and the Yangtze Block. | ZENG Wen ZHANG Li ZHOU HanWen ZHONG ZengQiu XIANG Hua LIU Rui JIN Song LU XinQian LI ChunZhong | 2008 | Chinese Science Bulletin2008，53，6： | 21 |
| 8 | Gene therapy for human colorectal carcinoma using human CEA promoter controlled bacterial ADP-ribosylating toxin genes:PEA and DTA gene transfer显示文摘INTRODUCTIONAmajorrequirementforinvivogenetherapyforcanceristotargetthetherapeuticgeneexpresiontomalignanttissuesbyselectingp... | CAO Guang Wen 1, QI Zhong Tian 1, PAN Xin 1, ZHANG Xiao Qin 1, MIAO Xiao Hui 1, FENG Yan 1, LU Xin Hua 1, Shigeki Kuriyama 2 and DU Ping 1 | 1998 | World Journal of Gastroenterology1998，4，5： | 18 |
| 9 | SHP2 inhibition triggers anti-tumor immunity and synergizes with PD-1 blockade显示文摘Tyrosine phosphatase SHP2 is a promising drug target in cancer immunotherapy due to its bidirectional role in both tumor growth promotion and T-cell inactivation. Its allosteric inhibitor SHP099 is known to inhibit cancer cell growth both in vitro and in vivo. However, whether SHP099-mediated SHP2 inhibition retards tumor growth in vivo via anti-tumor immunity remains elusive. To address this, a CT-26 colon cancer xenograft model was established in mice since this cell line is insensitive to SHP099.Consequently, SHP099 minimally affected CT-26 tumor growth in immuno-deficient nude mice, but significantly decreased the tumor burden in CT-26 tumor-bearing mice with intact immune system.SHP099 augmented anti-tumor immunity, as shown by the elevated proportion of CD8tIFN-γtT cells and the upregulation of cytotoxic T-cell related genes including Granzyme B andPerforin, which decreased the tumor load. In addition, tumor growth in mice with SHP2-deficient T-cells was markedly slowed down because of enhanced anti-tumor responses. Finally, the combination of SHP099 and antiPD-1 antibody showed a higher therapeutic efficacy than either monotherapy in controlling tumor growthin two colon cancer xenograft models, indicating that these agents complement each other. Our study suggests that SHP2 inhibitor SHP099 is a promising candidate drug for cancer immunotherapy. | Mingxia Zhao Wenjie Guo Yuanyuan Wu Chenxi Yang Liang Zhong Guoliang Deng Yuyu Zhu Wen Liu Yanhong Gu Yin Lu Lingdong Kong Xiangbao Meng Qiang Xu Yang Sun | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 18 |
| 10 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 11 | Contrast-enhanced ultrasound:Improving the preoperative staging of hepatocellular carcinoma and guiding individual treatment显示文摘AIM:To investigate the clinical role of contrast-enhanced ultrasound(CEUS) combined with contrast-enhanced computed tomography(CE-CT) or magnetic resonance imaging to improve the preoperative staging of hepatocellular carcinoma(HCC) and guide surgical decisionmaking.METHODS:Sixty-nine patients who underwent liver resection for HCC in our center were enrolled prospectively in the study.CEUS and CE-CT/MRI were performed before surgery.Intraoperative ultrasound(IOUS)wascarried out after liver mobilization.Lesions depicted by each imaging modality were counted and mapped.To investigate the impact of tumor size on the study,we divided the patients into two groups,the'Smaller group'(S-group,≤5 cm in diameter)and the'Largergroup'(L-group,>5 cm in diameter).The sensitivity,specificity,accuracy,positive predictive value,and negative predictive value of CE-CT/MRI,CEUS,IOUS,CEUS+CE-CT/MRI and the tumor node metastasis staging of tumors were calculated and compared.Changes in the surgical strategy as a result of CEUS and IOUS were analyzed.RESULTS:One hundred and twenty-seven nodules,comprising 94 HCCs confirmed by histopathology and33 benign lesions confirmed by histopathology and follow-up,were identified in 69 patients.The overall diagnostic sensitivity rates of CE-CT/MRI,CEUS,IOUS and CEUS+CE-CT/MRI were 78.7%,89.4%,89.4%and 89.4%,respectively.There was a significant difference between CEUS+CE-CT/MRI and CE-CT/MRI(P=0.046).Combining CEUS with CT or MRI increased,the diagnostic specificity compared with CT/MRI,CEUS and IOUS,and this difference was statistically significant(100%,72.7%,97.0%,and 69.7%,P=0.004,P=0.002,P=0.002,respectively).The diagnostic accuracy was significantly higher for CEUS+CT/MRI compared with CT/MRI(92.1%vs 77.2%,P=0.001).The TNM staging of tumors based on CEUS+CE-CT/MRI approximated to the final pathological TNM staging(P=0.977).There was a significant difference in the accuracy of TNM staging when comparing CEUS+CECT/MRI with CE-CT/MRI(P=0.002).Before surgery,strategies were changed in 15.9%(11/69)of patients as a result of CEUS.Finally,only 5.7%(4/69)of surgical strategies were changed because of IOUS findings.In the S-group,CEUS revealed 12 false positive lesions,including seven false positive lesions that were diagnosedby preoperative imaging examinations and five by IOUS.In contrast,in the L-group,IUOS revealed eight new malignant lesions;six of these lesions were true HCCs that were also identified by preoperative CEUS.CONCLUSION:CEUS combined with CT or MRI improves the accuracy of preoperative staging for hepatocellular carcinoma and may help to guide individualized treatment for patients with HCC.CEUS may better identify non-malignant lesions in patients with small tumors and discover new malignant lesions in patients with large tumors. | Xiao-Yun Zhang Yan Luo Tian-Fu Wen Li Jiang Chuan Li Xiao-Fei Zhong Jing-Yi Zhang Wen-Wu Ling Lu-Nan Yan Yong Zeng Hong Wu | 2014 | World Journal of Gastroenterology2014，20，35： | 16 |
| 12 | Combined MELD and blood lipid level in evaluating the prognosis of decompensated cirrhosis显示文摘AIM: To evaluate the prognostic value of the combined model for end-stage liver disease (MELD) and blood lipid level in patients with decompensated cirrhosis. METHODS: A total of 198 patients with decompensated cirrhosis were enrolled into the study. The values of triglyceride (TG), cholesterol (TC), high density lipoproteins (HDL) and low density lipoprotein (LDL) of each patient on the fi rst day of admission were retrieved from the medical records, and MELD was calculated. All the patients were followed up for 1 year. The relationship between the change of blood lipid level and the value of MELD score was studied by analysis of variance. The prognostic factors were screened by multivariate Cox proportional hazard model. Draw Kaplan-Meier survival curves were drawn. RESULTS: Forty-f ive patients died within 3 mo and 83 patients died within 1 year. The levels of TG, TC, HDL and LDL of the death group were all lower than those of the survivors. The serum TG, TC, HDL and LDL levels were lowered with the increase of the MELD score. Multivariate Cox proportional hazard model showed that MELD ≥18 and TC ≤2.8 mmol/L were independent risk factors for prognosis of decompensated cirrhosis. Survival analysis showed that MELD ≥18 combined with TC ≤ 2.8 mmol/L can clearly discriminate between the patients who would survive and die in 1 year. CONCLUSION: MELD ≥18 and TC ≤2.8 mmol/L are two important indexes to predict the prognosis of patients with decompensated cirrhosis. Their combination can effectively predict the long-term prognosis of patients with decompensated cirrhosis. | Jiang, Ming Liu, Fei Xiong, Wu-Jun Zhong, Lan Xu, Wen Xu, Fei Liu, Yan-Bing | 2010 | World Journal of Gastroenterology2010，16，11： | 14 |
| 13 | Effects of Naoxintong on atherosclerosis and inducible nitric oxide synthase expression in atherosclerotic rabbit显示文摘 | ZHONG Xiao-nan WANG Hong-hao LU Zheng-qi DAI Yong-qiang HUANG Jian-hua QIU Wei SHU Ya-qing XU Wen CHENG Chen HU Xue-qiang | 2013 | Chinese Medical Journal2013，，6： | 14 |
| 14 | Genome of Wild Mandarin and Domestication History of Mandarin显示文摘官员(柠檬 reticulata ) 是世界范围的最重要的柠檬庄稼之一。它的驯服被相信发生在华南,它是四几千年的官员耕作的中心之一。我们在 Nanling 区域附近收集了官员的自然野人口并且在附近栽培了 landraces。我们发现柠檬性的酸水平戏剧性地在栽培官员被减少。理解 ? 官员驯服的基因基础,我们 de novo 装配了野官员的一个草稿染色体并且分析了一套 104 个柠檬染色体。我们发现 Mangshan 官员是一种原始类型并且二个独立驯服事件发生了,分别地导致在北方和南方 Nanling 山的二组栽培官员(MD1 和 MD2 ) 。二个瓶颈和有效人口尺寸的二扩大为栽培官员的 MD1 组被识别。然而,在 MD2 组织 ? 在人口尺寸有长、连续的减少。MD1 和 MD2 官员从栽培 pummelo 种类显示出种间的基因渗入的不同模式。我们在官员的驯服期间在选择下面可能在基因在柠檬酸盐内容的规定包含了的 aconitate hydratase (ACO ) 识别了高分叉的一个区域,它是。这研究为现存野官员人口的地理起源提供具体基因证据并且使驯服和官员的进化历史清楚些。 | Lun Wang Fa He Yue Huang Jiaxian He Shuizhi Yang Jiwu Zeng Chongling Deng Xiaolin Jiang Yiwen Fang Shaohua Wen Rangwei Xu Huiwen Yu Xiaoming Yang Guangyan Zhong Chuanwu Chen Xiang Yan Changfu Zhou Hongyan Zhang Zongzhou Xie Robert M. Larkin Xiuxin Deng Qiang Xu | 2018 | Molecular Plant2018，11，8： | 14 |
| 15 | Molecular Typing of Brucella Suis Collected from 1960s to 2010s in China by MLVA and PFGE显示文摘Brucellosis is a bacterial anthropozoonosis usually caused by Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. Brucella suis, the causative agent of swine brucellosis, is classified into five biovars and preferentially infects different animal hosts [1] . | LI Zhen Jun CUI Bu Yun CHEN Hai CHEN Jing Diao ZHAO Hong Yan PIAO Dong Ri JIANG Hai ZHANG Li TANG Xu KE Chang Wen YAO zhen TIAN Guo Zhong | 2013 | Biomedical and Environmental Sciences2013，26，6： | 13 |
| 16 | Chemoresistance of CD133+ cancer stem cells in laryngeal carcinoma显示文摘背景增长证据建议肿瘤是组织学地异构的并且被肿瘤房间的一张小人口维持称为的癌症干细胞。CD133 在喉的癌作为癌症干细胞的一个候选人标记被识别了。这研究试图分析 CD133+ 癌症茎 cells.Methods 的 chemoresistance 到不同化学疗法的代理人的 Hep-2 细胞的反应被调查, CD133 的表示被学习。激活荧光的房间排序分析在殖民地形成试金被用来识别 CD133 ,并且房间的CD133+子集被分开并且分析,房间侵略试金,化疗抵抗研究,并且关于 Hep-2 房间的1%-2%为药抵抗基因 ABCG2.Results 的表示分析了是CD133+房间,并且CD133+比例被chemotherapy.CD133+癌症干细胞充实为 clonogenicity 和侵略展出了更高的潜力,并且对化疗更抵抗。这个电阻与这研究建议了的 ABCG2.Conclusions 的更高的表示被相关 CD133+ 癌症干细胞对化疗更抵抗。ABCG2 的表示能为这部分负责。指向 CD133+ 癌症干细胞的这张小人口能是策略为喉的癌开发更有效的治疗。 | YANG Jing-pu LIU Yan ZHONG Wei YU Dan WEN Lian-ji JIN Chun-shun | 2011 | Chinese Medical Journal2011，，7： | 12 |
| 17 | Minimizing tacrolimus decreases the risk of new-onset diabetes mellitus after liver transplantation显示文摘AbstractAIM: To investigate the impact of minimum tacrolimus(TAC) on new-onset diabetes mellitus (NODM) afterliver transplantation (LT).METHODS: We retrospectively analyzed the data of973 liver transplant recipients between March 1999and September 2014 in West China Hospital LiverTransplantation Center. Following the exclusion ofineligible recipients, 528 recipients with a TAC-dominantregimen were included in our study. We calculatedand determined the mean trough concentration ofTAC (cTAC) in the year of diabetes diagnosis in NODMrecipients or in the last year of the follow-up in non-NODM recipients. A cutoff of mean cTAC value forpredicting NODM 6 mo after LT was identified usinga receptor operating characteristic curve. TAC-relatedcomplications after LT was evaluated by χ^2 test, andthe overall and allograft survival was evaluated usingthe Kaplan-Meier method. Risk factors for NODM afterLT were examined by univariate and multivariate Cox regression.RESULTS: Of the 528 transplant recipients, 131(24.8%) developed NODM after 6 mo after LT, andthe cumulative incidence of NODM progressivelyincreased. The mean cTAC of NODM group recipientswas significantly higher than that of recipients in thenon-NODM group (7.66 ± 3.41 ng/mL vs 4.47 ± 2.22ng/mL, P 〈 0.05). Furthermore, NODM group recipientshad lower 1-, 5-, 10-year overall survival rates (86.7%,71.3%, and 61.1% vs 94.7%, 86.1%, and 83.7%, P 〈0.05) and allograft survival rates (92.8%, 84.6%, and75.7% vs 96.1%, 91%, and 86.1%, P 〈 0.05) thanthe others. The best cutoff of mean cTAC for predictingNODM was 5.89 ng/mL after 6 mo after LT. Multivariateanalysis showed that old age at the time of LT (〉 50years), hypertension pre-LT, and high mean cTAC (≥5.89 ng/mL) after 6 mo after LT were independent riskfactors for developing NODM. Concurrently, recipientswith a low cTAC (〈 5.89 ng/mL) were less likely tobecome obese (21.3% vs 30.2%, P 〈 0.05) or todevelop dyslipidemia (27.5% vs 44.8%, P 〈0.05),chronic kidney dysfunction (14.6% vs 22.7%, P 〈 0.05),and moderate to severe infection (24.7% vs 33.1%, P〈 0.05) after LT than recipients in the high mean cTACgroup. However, the two groups showed no significantdifference in the incidence of acute and chronicrejection, hypertension, cardiovascular events and newonsetmalignancy.CONCLUSION: A minimal TAC regimen can decreasethe risk of long-term NODM after LT. Maintaining a cTACvalue below 5.89 ng/mL after LT is safe and beneficial. | Jiu-Lin Song Wei Gao Yan Zhong Lu-Nan Yan Jia-Yin Yang Tian-Fu Wen Bo Li Wen-Tao Wang Hong Wu Ming-Qing Xu Zhe-Yu Chen Yong-Gang Wei Li Jiang Jian Yang | 2016 | World Journal of Gastroenterology2016，22，6： | 12 |
| 18 | A Novel Feature Selection Method Based on Probability Latent Semantic Analysis for Chinese Text Classification显示文摘 | ZHONG Jiang SUN Qigan LI Xue WEN Luosheng | 2011 | Chinese Journal of Electronics2011，20，2： | 11 |
| 19 | Construction of HBV-specific ribozyme and its recombinant with HDV and their cleavage activity in vitro显示文摘AIM To construct the recombinant of HDVcDNA and HBV-specific ribozyme gene byrecombinant PCR in order to use HDV as atransporting vector carrying HBV-specificribozyme into liver cells for inhibiting thereplication of HBV.METHODS We separately cloned the ribozyme(RZ)gene and recombinant DVRZ(comprisingHDV cDNA and HBV-specific ribozyme gene)intothe downstream of T7 promoter of pTAdv-Tvector and studied the in vitro cleavage activityof their transcripts(rRZ,rDVRZ)on target RNA(rBVCF)from in vitro transcription of HBV Cgene fragment(BVCF).RESULTS Both the simple(rRZ)and therecombinant ribozyme rDVRZ could efficientlycatalyze the cleavage of target RNA(rBVCF)under different temperatures(37℃,42℃ and55℃)and Mg2+concentrations(10 mmol/L,15 mmol/L and 20 mmol/L)and their catalyticactivity tended to increase as the temperaturewas rising.But the activity of rRZ was evidentlyhigher than that of rDVRZ.CONCLUSION The recombinant of HDV cDNAand ribozyme gene had the potential of beingfurther explored and used in gene therapy of HBVinfection. | Shu Juan Wen Kai Jun Xiang Zhen Hua Huang Rong Zhou Xue Zhong Qi | 2000 | World Journal of Gastroenterology2000，6，3： | 10 |
| 20 | Establishment of transgenic mouse harboring hepatitis B virus (adr subtype) genomes显示文摘INTRODUCTIONHepatitis B virus (HBV) belongs to the group ofhepatovirus, a major pathogen of human acute andchronic hepatitis B[1 4], which has a very closeassociation with human hepatocellular carcinoma(HCC)[5-8], For example, a statistical data from ahospital in Shanghai showed that 80% of HCCpatients were positive for HBsAg ( personalcommunication). | Yi Ping Hu1 Wei Jiang Hu1 Wen Chao Zheng2 Jian Xiu Li1 De Shun Dai1 Xin Min Wang1 Shu Zhong Zhang1 Hong Yu Yu3 Wei Sun4 Guang Rong Hao4 1Department of Cell Biology, Second Military Medical University, Shanghai 200433, China2University of Wisconsin, Madison, WI 53705, USA3Department of Pathology, Second Military Medical University, Shanghai 200433, China4Center of laboratory Animals, Second Military Medical University, Shanghai 200433, China | 2001 | World Journal of Gastroenterology2001，7，1： | 9 |
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