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| 1 | Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules显示文摘由病毒的 transduction 的四抄写因素 Oct4, Klf4, Sox2 和 c-Myc 的介绍能导致体的房间的 reprogramming 进导致的 pluripotent 干细胞(iPSCs ) ,但是 iPSCs 的使用被病毒的交货系统的使用妨碍。导致化学药品的 reprogramming 把一条新奇途径提供给没有任何病毒的基于向量的基因修正,产生 iPSCs。尽管,以前的报告证明几个小分子能代替一些 reprogramming 因素至少二个抄写因素, Oct4 和 Klf4,仍然被要求从老鼠产生 iPSCs 胚胎的成纤维细胞。这里,我们识别特定的化学联合,它是足够的面对一个单个抄写因素从胚胎的老鼠和成年成纤维细胞允许 reprogramming, Oct4 在 20 天以内,代替 Sox2, Klf4 和 c-Myc。用这个处理产生的 iPSCs 类似于胚胎的干细胞以全球基因表示介绍的老鼠,在 vitro 并且在 vivo 的 epigenetic 地位和 pluripotency。我们也发现那 8 天 Oct4 正式就职是足够的面对小分子启用导致 Oct4 的 reprogramming,它建议 reprogramming 在开始的 8 天以内被开始并且独立于连续外长的 Oct4 表示。没有基因修正,这些发现将帮助 iPSCs 的未来产生,以及阐明位于 reprogramming 过程下面的分子的机制。 | Yanqin Li Qiang Zhang Xiaolei Yin Weifeng Yang Yuanyuan Du Pingping Hou Jian Ge Chun Liu Weiqi Zhang Xu Zhang Yetao Wu Honggang Li Kang Liu Chen Wu Zhihua Song Yang Zhao Yan Shi Hongkui Deng | 2011 | Cell Research2011，21，1： | 51 |
| 2 | SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2显示文摘 | Huize Pan Di Guan Xiaomeng Liu Jingyi Li Lixia Wang Jun Wu Junzhi Zhou Weizhou Zhang Ruotong Ren Weiqi Zhang Ying Li Jiping Yang Ying Hao Tingting Yuan Guohong Yuan Hu Wang Zhenyu Ju Zhiyong Mao Jian Li Jing Qu FuchouTang Guang-Hui Liu | 2016 | Cell Research2016，26，2： | 32 |
| 3 | Ambient volatile organic compounds pollution in China显示文摘Owing to rapid economic and industrial development,China has been suffering from degraded air quality and visibility. Volatile organic compounds(VOCs)are important precursors to the formation of ground-level ozone and hence photochemical smog. Some VOCs adversely affect human health. Therefore,VOCs have recently elicited public concern and given new impetus to scientific interest. China is now implementing a series of polices to control VOCs pollution. The key to formulating policy is understanding the ambient VOCs pollution status. This paper mainly analyzes the species,levels,sources,and spatial distributions of VOCs in ambient air. The results show that the concentrations of ambient VOCs in China are much higher than those of developed countries such as the United States and Japan,especial benzene,which exceeds available standards. At the same time,the ozone formation potential(OFP)and secondary organic aerosol formation potential(SOAFP)of various VOCs are calculated. Aromatics and alkenes have much higher OFPs,while aromatics have higher SOAFP. The OFPs of ambient VOCs in the cities of Beijing,Guangzhou and Changchun are very high,and the SOAFP of ambient VOCs in the cities of Hangzhou,Guangzhou and Changchun are higher. | Xinmin Zhang Zhigang Xue Hong Li Li Yan Yuan Yang Yi Wang Jingchun Duan Lei Li Fahe Chai Miaomiao Cheng Weiqi Zhang | 2017 | Journal of Environmental Sciences2017，29，5： | 34 |
| 4 | miR-106b-25/miR-17-92 clusters: Polycistrons with oncogenic roles in hepatocellular carcinoma显示文摘MicroRNAs are small endogenously expressed RNA molecules which are involved in the process of silencing gene expression through translational regulation.The polycistronic miR-17-92 cluster is the first microRNA cluster shown to play a role in tumorigenesis.It has two other paralogs in the human genome,the miR-106b-25 cluster and the miR-106a-363 cluster.Collectively,the microRNAs encoded by these clusters can be further grouped based on the seed sequences into four families,namely the miR-17,the miR-92,the miR-18and the miR-19 families.Over-expression of the miR-106b-25 and miR-17-92 clusters has been reported not only during the development of cirrhosis but also subsequently during the development of hepatocellular carcinoma.Members of these clusters have also been shown to affect the replication of hepatitis B and hepatitis C viruses.Various targets of these microRNAs have been identified,and these targets are involved in tumor growth,cell survival and metastasis.In this review,we first describe the regulation of these clusters by c-Myc and E2F1,and how the members of these clusters inturn regulate E2F1 expression forming an auto-regulatory loop.In addition,the roles of the various members of the clusters in affecting relevant target gene expression in the pathogenesis of hepatocellular carcinoma will also be discussed. | Weiqi Tan Yang Li Seng-Gee Lim Theresa MC Tan | 2014 | World Journal of Gastroenterology2014，20，20： | 17 |
| 5 | Size-resolved aerosol water-soluble ions at a regional background station of Beijing, Tianjin, and Hebei, North China显示文摘The characteristics of water-soluble ions in size-resolved particulate matter were investigated usingion chromatography at Shangdianzi,a regional background station of Beijing,Tianjin,and Hebei.Seasonal total concentrations of ions(Na^+,Mg^(2+),K^+,Ca^(2+),NH_4^+,Cl^-,SO_4^(2-) and NO_3^-) were75.5±52.9 μg/m^3 in spring,26.5±12.3μg/m^3 in summer,22.7±20.4μg/m^3 in autumn,and31.1±23.9 μg/m^3 in winter,respectively.The secondary ions(NO_3^-,SO_4^(2-) and NH_4^+),mainly associated with fine particles,accounted for 84.2%in spring,82.1%in summer,81.5%in autumn and 76.3%in winter of all ions.Strong correlations were found between NH_4^+ and SO_4^(2-)(r=0.95,p<0.01) as well as NH_4^+ and NO_3^-(r=0.90,p<0.01) in fine particles;while in coarse particles,correlations between Mg^(2+) and NO_3^-(r=0.80,p<0.01),and Ca^(2+) and NO_3^-(r=0.85,p<0.01) were found.The concentrations of Na^+,K^+,Mg^(2+),Ca^(2+),NH_4^+,Cl^-,NO_3^-,and SO_4^(2-) were 2.02,0.81,0.36,1.65,9.58,4.01,18.9,and 18.4 μg/m^3 in particulate matter from southeast-derived air masses,which were typically 1.58-3.37 times higher than in northwest trajectories.Thus,concentrations of water-soluble ions at this background station were heavily influenced by regional transport of serious pollution derived from biomass burning,coal combustion,industrial and vehicle exhaust emissions from Beijing,Tianjin,and Hebei. | Yongjie Yang Rui Zhou Yue Yu Yan Yan Yan Liu Yi'an Di Dan Wu Weiqi Zhang | 2017 | Journal of Environmental Sciences2017，29，5： | 16 |
| 6 | Genetic enhancement in cultured human adult stem cells conferred by a single nucleotide recoding显示文摘 | Jiping Yang Jingyi Li Keiichiro Suzuki Xiaomeng Liu Jun Wu Weiqi Zhang Ruotong Ren Weizhou Zhang Piu Chan Juan Carlos Izpisua Belmonte Jing Qu Fuchou Tang Guang-Hui Liu | 2017 | Cell Research2017，27，9： | 12 |
| 7 | Modeling xeroderma pigmentosum associated neurological pathologies with patients-derived iPSCs显示文摘干皮病 pigmentosum (XP ) 是联系 XP 的基因的变化引起的一组基因混乱,导致 DNA 修理的缺陷。XP 病人经常展出神经病学的退化,而是内在的机制是未知的,部分地由于合适的疾病模型的缺乏。这里,我们产生了包括 XPA, XPB, XPC, XPG,和 XPV 在五不同 XP 基因怀有变化的病人特定的导致的 pluripotent 干细胞(iPSCs ) 。这些 iPSCs 进一步被区分到神经房间,并且他们到 DNA 损坏应力的危险性被调查。在神经干细胞(NSC ) 或神经原的 XPA 的变化导致了严重 DNA 损坏修理缺点,并且有变异的 XPA 的这些神经房间对 DNA 导致损坏的 apoptosis 过分敏感。因此, XP 变异的神经房间代表珍贵工具在 XP 病人澄清神经病学的畸形的分子的机制。 | Lina Fu Xiuling Xu Ruotong Ren Jun Wu Weiqi Zhang Jiping Yang Xiaoqing Ren Si Wang Yang Zhao Liang Sun Yang Yu Zhaoxia Wang Ze Yang Yun Yuan Jie Qiao Juan Carlos Izpisua Belmonte Jing Qu Guang-Hui Liu | 2016 | Protein & Cell2016，7，3： | 11 |
| 8 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
| 9 | Single-cell transcriptomic atlas of primate cardiopulmonary aging显示文摘Aging is a major risk factor for many diseases,especially in highly prevalent cardiopulmonary comorbidities and infectious diseases including Coronavirus Disease 2019(COVID-19).Resolving cellular and molecular mechanisms associated with aging in higher mammals is therefore urgently needed.Here,we created young and old non-human primate single-nucleus/cell transcriptomic atlases of lung,heart and artery,the top tissues targeted by SARS-CoV-2.Analysis of cell type-specific aging-associated transcriptional changes revealed increased systemic inflammation and compromised virus defense as a hallmark of cardiopulmonary aging.With age,expression of the SARS-CoV-2 receptor angiotensin-converting enzyme 2(ACE2)was increased in the pulmonary alveolar epithelial barrier,cardiomyocytes,and vascular endothelial cells.We found that interleukin 7(IL7)accumulated in aged cardiopulmonary tissues and induced ACE2 expression in human vascular endothelial cells in an NF-κB-dependent manner.Furthermore,treatment with vitamin C blocked IL7-induced ACE2 expression.Altogether,our findings depict the first transcriptomic atlas of the aged primate cardiopulmonary system and provide vital insights into age-linked susceptibility to SARS-CoV-2,suggesting that geroprotective strategies may reduce COVID-19 severity in the elderly. | Shuai Ma Shuhui Sun Jiaming Li Yanling Fan Jing Qu Liang Sun Si Wang Yiyuan Zhang Shanshan Yang Zunpeng Liu Zeming Wu Sheng Zhang Qiaoran Wang Aihua Zheng Shuguang Duo Yang Yu Juan Carlos Izpisua Belmonte Piu Chan Qi Zhou Moshi Song Weiqi Zhang Guang-Hui Liu | 2021 | Cell Research2021，31，4： | 10 |
| 10 | Secretory/releasing proteome-based identification of plasma biomarkers in HBV-associated hepatocellular carcinoma显示文摘For successful therapy, hepatocellular carcinoma (HCC) must be detected at an early stage. Herein, we used a proteomic approach to analyze the secretory/releasing proteome of HCC tissues to identify plasma biomarkers. Serum-free conditioned media (CM) were collected from primary cultures of cancerous tissues and surrounding noncancerous tissues. Proteomic analysis of the CM proteins permitted the identification of 1365 proteins. The enriched molecular functions and biological processes of the CM proteins, such as hydrolase activity and catabolic processes, were consistent with the liver being the most important metabolic organ. Moreover, 19% of the proteins were characterized as extracellular or membrane-bound. For validation, secretory proteins involved in transforming growth factor-β signaling pathways were validated in plasma samples. Alphafetoprotein (AFP), metalloproteinase (MMP)1, osteopontin (OPN), and pregnancy-specific beta-1-glycoprotein (PSG)9 were significantly increased in HCC patients. The overall performance of MMP1 and OPN in the diagnosis of HCC remained greater than that of AFP. In addition, this study represents the first report of MMP1 as a biomarker with a higher sensitivity and specificity than AFP. Thus, this study provides a valuable resource of the HCC secretome with the potential to investigate serological biomarkers. MMP1 and OPN could be used as novel biomarkers for the early detection of HCC and to improve the sensitivity of biomarkers compared with AFP. | YANG Lei RONG WeiQi XIAO Ting ZHANG Ying XU Bin LIU Yu WANG LiMing WU Fan QI Jun ZHAO XiuYing WANG HongXia HAN NaiJun GUO SuPing WU JianXiong GAO YanNing CHENG ShuJun | 2013 | Science China(Life Sciences)2013，56，7： | 9 |
| 11 | Rescue of premature aging defects in Cockayne syndrome stem cells by CRISPR/Cas9-mediated gene correction显示文摘Cockayne syndrome(CS)is a rare autosomal recessive inherited disorder characterized by a variety of clinical features,including increased sensitivity to sunlight,progressive neurological abnormalities,and the appearance of premature aging.However,the pathogenesis of CS remains unclear due to the limitations of current disease models.Here,we generate integration-free induced pluripotent stem cells(iPSCs)from fibroblasts from a CS patient bearing mutations in CSB/ERCC6 gene and further derive isogenic genecorrected CS-iPSCs(GC-iPSCs)using the CRISPR/Cas9 system.CS-associated phenotypic defects are recapitulated in CS-iPSC-derived mesenchymal stem cells(MSCs)and neural stem cells(NSCs),both of which display increased susceptibility to DNA damage stress.Premature aging defects in CS-MSCs are rescued by the targeted correction of mutant ERCC6.We next map the transcriptomic landscapes in CS-iPSCs and GC-iPSCs and their somatic stem cell derivatives(MSCs and NSCs)in the absence or presence of ultraviolet(UV)and replicative stresses,revealing that defects in DNA repair account for CS pathologies.Moreover,we generate autologous GC-MSCs free of pathogenic mutation under a cGMP(Current Good Manufacturing Practice)-compliant condition,which hold potential for use as improved biomaterials for future stem cell replacement therapy for CS.Collectively,our models demonstrate novel disease features and molecular mechanisms and lay a foundation for the development of novel therapeutic strategies to treat CS. | Si Wang Zheying Min Qianzhao Ji Lingling Geng Yao Su Zunpeng Liu Huifang Hu Lixia Wang Weiqi Zhang Keiichiro Suzuiki Yu Huang Puyao Zhang Tie-Shan Tang Jing Qu Yang Yu Guang-Hui Liu Jie Qiao | 2020 | Protein & Cell2020，11，1： | 7 |
| 12 | Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine显示文摘Mesenchymal stem cell(MSC)-mediated immunomodulation has been harnessed for the treatment of human diseases,but its underlying mechanism has not been fully understood.Dead cells,including apoptotic cells have immunomodulatory properties.It has been repeatedly reported that the proportion of nonviable MSCs in a MSC therapeutic preparation varied from 5-50%in the ongoing clinical trials.It is conceivable that the nonviable cells in a MSC therapeutic preparation may play a role in the therapeutic effects of MSCs.We found that the MSC therapeutic preparation in the present study had about 5%dead MSCs(DMSCs),characterized by apoptotic cells.Namely,1×10^(6) MSCs in the preparation contained about 5×10^(4) DMSCs.We found that the treatment with even 5×10^(4) DMSCs alone had the equal therapeutic effects as with 1×10^(6) MSCs.This protective effect of the dead MSCs alone was confirmed in four mouse models,including concanavalin A(ConA)-and carbon tetrachloride(CCI4)-induced acute liver injury,LPS-induced lung injury and spinal cord injury.We also found that the infused MSCs died by apoptosis in vivo.Furthermore,the therapeutic effect was attributed to the elevated level of phosphatidylserine(PS)upon the injection of MSCs or DMSCs.The direct administration of PS liposomes(PSLs)mimic apoptotic cell fragments also exerted the protective effects as MSCs and DMSCs.The Mer tyrosine kinase(MerTK)deficiency or the knockout of chemokine receptor C-C motif chemokine receptor 2(CCR2)reversed these protective effects of MSCs or DMSCs.These results revealed that DMSCs alone in the therapeutic stem cell preparation or the apoptotic cells induced in vivo may exert the same immunomodulatory property as the'living MSCs preparation'through releasing PS,which was further recognized by MerTK and participated in modulating immune cells. | Xuemei He Weiqi Hong Jingyun Yang Hong Lei Tianqi Lu Cai He Zhenfei Bi Xiangyu Pan Yu Liu Lunzhi Dai Wei Wang Canhua Huang Hongxin Deng Xiawei Wei | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 6 |
| 13 | TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling显示文摘Osteoporosis is an osteolytic disorder commonly associated with excessive osteoclast formation.Transcriptional coactivator with PDZ-binding motif(TAZ)is a key downstream effector of the Hippo signaling pathway;it was suggested to be involved in the regulation of bone homeostasis.However,the exact role of TAZ in osteoclasts has not yet been established.In this study,we demonstrated that global knockout and osteoclast-specific knockout of TAZ led to a low-bone mass phenotype due to elevated osteoclast formation,which was further evidenced by in vitro osteoclast formation assays.Moreover,the overexpression of TAZ inhibited RANKL-induced osteoclast formation,whereas silencing of TAZ reduced it.Mechanistically,TAZ bound to TGF-activated kinase 1(TAK1)and reciprocally inhibited NF-κB signaling,suppressing osteoclast differentiation.Collectively,our findings highlight an essential role of TAZ in the regulation of osteoclastogenesis in osteoporosis and its underlying mechanism. | Wanlei Yang Xuanyuan Lu Tan Zhang Weiqi Han Jianlei Li Wei He Yewei Jia Kangxian Zhao An Qin Yu Qian | 2021 | Bone Research2021，9，3： | 6 |
| 14 | High-performance asymmetric electrodes photodiode based on Sb/WSe2 heterostructure显示文摘Two-dimensional (2D) van der Waals (vdWs) metal-semiconductor heterostructures with atomically sharp interface and matched work functions have recently attracted great attention due to their unique electronic and optoelectronic properties. Here we report the vapor phase epitaxial growth of large-scale vertical Sb/WSe2 metal-semiconductor vdWs heterostructures with uniform stacking orientation. Compared with the growth on S1O2/S1 substrate, the thick ness of Sb nan osheet on WSe2 can be reduced effectively to mono layer. We con struct Sb-WSe2-Au asymmetric electrodes photodiode based on the Sb/WSe2 heterostructures. Electrical transport measurements indicate that the photodiode show obvious rectifying effect. Optoelectronic characterizations show prominent photoresponse with a high photoresposivity of 364 mA/W, a fast response time of less than 8 ms, a large open-circuit voltage of 0.27 V and a maximum electrical power output of 0.11 nW. The direct growth of high-quality metal-semiconductor vdWs heterostructures may open up new realms in 2D functional electronics and optoelectronics. | Xiao Liu Guangzhuang Sun Peng Chen Junchi Liu Zhengwei Zhang Jia Li Huifang Ma Bei Zhao Ruixia Wu Weiqi Dang Xiangdong Yang Chen Dai Xuwan Tang Zhuojun Chen Lili Miao Xingqiang Liu Bo Li Yuan Liu Xidong Duan | 2019 | Nano Research2019，12，2： | 5 |
| 15 | Applications of genome editing technology in the targeted therapy of human diseases:mechanisms,advances and prospects显示文摘Based on engineered or bacterial nucleases,the development of genome editing technologies has opened up the possibility of directly targeting and modifying genomic sequences in almost all eukaryotic cells.Genome editing has extended our ability to elucidate the contribution of genetics to disease by promoting the creation of more accurate cellular and animal models of pathological processes and has begun to show extraordinary potential in a variety of fields,ranging from basic research to applied biotechnology and biomedical research.Recent progress in developing programmable nucleases,such as zinc-finger nucleases(ZFNs),transcription activator-like effector nucleases(TALENs)and clustered regularly interspaced short palindromic repeat(CRISPR)–Cas-associated nucleases,has greatly expedited the progress of gene editing from concept to clinical practice.Here,we review recent advances of the three major genome editing technologies(ZFNs,TALENs,and CRISPR/Cas9)and discuss the applications of their derivative reagents as gene editing tools in various human diseases and potential future therapies,focusing on eukaryotic cells and animal models.Finally,we provide an overview of the clinical trials applying genome editing platforms for disease treatment and some of the challenges in the implementation of this technology. | Hongyi Li Yang Yang Weiqi Hong Mengyuan Huang Min Wu Xia Zhao | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 16 | Post-surgical resection prognostic value of combined OPN, MMP7, and PSG9 plasma biomarkers in hepatocellular carcinoma显示文摘Biomarkers for hepatocellular carcinoma (HCC) following curative resection are not currently sufficient for prognostic indication of overall survival (OS) and disease-free survival (DFS). The aim of this study was to investigate the prognostic performance of osteopontin (OPN), matrix metalloproteinase 7 (MMP7), and pregnancy specific glycoprotein 9 (PSG9) in patients with HCC. A total of 179 prospective patients with HCC provided plasma before hepatectomy. Plasma OPN, MMP7, and PSG9 levels were determined by enzyme-linked immunosorbent assay. Correlations between plasma levels, clinical parameters, and outcomes (OS and DFS) were overall analyzed. High OPN (≥149.97 ng/mL), MMP7 (≥2.28 ng/mL), and PSG9 (≥45.59 ng/mL) were prognostic indicators of reduced OS (P<0.001, P<0.001, and P=0.007, respectively). Plasma PSG9 protein level was an independent factor in predicting OS (P=0.008) and DFS (P=0.038). Plasma OPN+MMP7+PSG9 elevation in combination was a prognostic factor for OS (P<0.001). OPN was demonstrated to be a risk factor-associated OS in stage I patients with HCC and patients with low α-fetoprotein levels (<20 ng/mL). These findings suggested that OPN, MMP7, PSG9 and their combined panels may be useful for aiding in tumor recurrence and mortality risk prediction of patients with HCC, particularly in the early stage of HCC carcinogenesis. | Weiqi Rong Yang Zhang Lei Yang Lin Feng Baojun Wei Fan Wu Liming Wang Yanning Gao Shujun Cheng Jianxiong Wu Ting Xiao | 2019 | Frontiers of Medicine2019，13，2： | 5 |
| 17 | A widely adaptable approach to generate integration-free iPSCs from non-invasively acquired human somatic cells显示文摘 | Zhichao Ding Lina Sui Ruotong Ren Yanjun Liu Xiuling Xu Lina Fu Ruijun Bai Tingting Yuan Ying Hao Weiqi Zhang Huize Pan Wensu Liu Han Yu Concepcion Rodriguez Esteban Xiaobing Yu Ze Yang Jian Li Xiaomin Wang Juan Carlos Izpisua Belmonte Guang-Hui Liu Fei Yi Jing Qu | 2015 | Protein & Cell2015，6，5： | 5 |
| 18 | Single-nucleus transcriptomic landscape of primate hippocampal aging显示文摘The hippocampus plays a crucial role in learning and memory,and its progressive deterioration with age is functionally linked to a variety of human neurodegenerative diseases.Yet a systematic profiling of the aging effects on various hippocampal cell types in primates is still missing.Here,we reported a variety of new aging-associated phenotypic changes of the primate hippocampus.These include,in particular,increased DNA damage and heterochromatin erosion with time,alongside loss of proteostasis and elevated inflammation.To understand their cellular and molecular causes,we established the first single-nucleus transcriptomic atlas of primate hippocampal aging.Among the 12 identified cell types,neural transiently amplifying progenitor cell(TAPC)and microglia were most affected by aging.In-depth dissection of gene-expression dynamics revealed impaired TAPC division and compromised neuronal function along the neurogenesis trajectory;additionally elevated pro-inflammatory responses in the aged microglia and oligodendrocyte,as well as dysregulated coagulation pathways in the aged endothelial cells may contribute to a hostile microenvironment for neurogenesis.This rich resource for understanding primate hippocampal aging may provide potential diagnostic biomarkers and therapeutic interventions against age-related neurodegenerative diseases. | Hui Zhang Jiaming Li Jie Ren Shuhui Sun Shuai Ma Weiqi Zhang Yang Yu Yusheng Cai Kaowen Yan Wei Li Baoyang Hu Piu Chan Guo-Guang Zhao Juan Carlos Izpisua Belmonte Qi Zhou Jing Qu Si Wang Guang-Hui Liu | 2021 | Protein & Cell2021，12，9： | 5 |
| 19 | The landscape of aging显示文摘Aging is characterized by a progressive deterioration of physiological integrity,leading to impaired functional ability and ultimately increased susceptibility to death.It is a major risk factor for chronic human diseases,including cardiovascular disease,diabetes,neurological degeneration,and cancer.Therefore,the growing emphasis on “healthy aging” raises a series of important questions in life and social sciences.In recent years,there has been unprecedented progress in aging research,particularly the discovery that the rate of aging is at least partly controlled by evolutionarily conserved genetic pathways and biological processes.In an attempt to bring full-fledged understanding to both the aging process and age-associated diseases,we review the descriptive,conceptual,and interventive aspects of the landscape of aging composed of a number of layers at the cellular,tissue,organ,organ system,and organismal levels. | Yusheng Cai Wei Song Jiaming Li Ying Jing Chuqian Liang Liyuan Zhang Xia Zhang Wenhui Zhang Beibei Liu Yongpan An Jingyi Li Baixue Tang Siyu Pei Xueying Wu Yuxuan Liu Cheng-Le Zhuang Yilin Ying Xuefeng Dou Yu Chen Fu-Hui Xiao Dingfeng Li Ruici Yang Ya Zhao Yang Wang Lihui Wang Yujing Li Shuai Ma Si Wang Xiaoyuan Song Jie Ren Liang Zhang Jun Wang Weiqi Zhang Zhengwei Xie Jing Qu Jianwei Wang Yichuan Xiao Ye Tian Gelin Wang Ping Hu Jing Ye Yu Sun Zhiyong Mao Qing-Peng Kong Qiang Liu Weiguo Zou Xiao-Li Tian Zhi-Xiong Xiao Yong Liu Jun-Ping Liu Moshi Song Jing-Dong J.Han Guang-Hui Liu | 2022 | Science China(Life Sciences)2022，65，12： | 4 |
| 20 | van der Waals epitaxial growth of ultrathin metallic NiSe nanosheets on WSe2 as high performance contacts for WSe2 transistors显示文摘A prerequisite for widespread applications of atomically thin transition metal dichalcogenides in future electronics is to achieve reliable electrical contacts,which is of considerable challenge due to the difficulties in selectively doping and inevitable physical damages of these atomically thin materials during typical metal integration process.Here,we report the in situ growth of ultrathin metallic NiSe single crystals on WSe2 in which the metallic NiSe nanosheets function as the contact electrodes to WSe2,creating an interface that is essentially free from chemical disorder.The NiSe/WSe2 heterostructures also exhibit well-aligned lattice orientation between the two layers,forming a periodic Moire pattern.Electrical transport studies demonstrate that the NiSe nanosheets exhibit an excellent metallic feature,as evidenced by the extra-high electrical conductivity of up to 1.6×10^6 S-nf1.The WSe2 transistors with the NiSe contact show field-effect mobilities (/vFe) more than double that with Cr/Au electrodes.This study demonstrates an effective pathway to achieve reliable electrical contacts to the atomically thin 2D materials,and maybe readily extended for fabricating 2D/2D low-resistance contacts for a variety of transition metal dichalcogenides. | Bei Zhao Weiqi Dang Xiangdong Yang Jia Li Haihong Bao Kai Wang Jun Luo Zhengwei Zhang Bo Li Haipeng Xie Yuan Liu Xidong Duan | 2019 | Nano Research2019，12，7： | 4 |
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