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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | RUNX3 directly interacts with in-tracellular domain of Notch1 and suppresses Notch signaling inhepatocellular carcinoma cells显示文摘 | Cao J Chen Y Wu KC | 2010 | Exp Cell Res2010，316，2： | 1 |
| 3 | Prognostic significance of microvascular obstruction by magnetic resonance imaging in patients with acute myocardial infarction显示文摘 | Wu KC Zerhouni EA Judd RM | 1998 | Circulation1998，97，： | 1 |
| 4 | Oxidant-induced apoptosis in cultured human retinal pigment epithelial cells显示文摘 | Cai JY Wu M Nelson KC Sternberg P Jones DP | 1999 | Invest Ophthalmol Vis Sci1999，40，： | 1 |
| 5 | Reliability, validity, and re- sponsiveness of Myotonometric measurement of muscle tone,elasticity, and stiffness in patients with stroke显示文摘 | Chuang LL Wu CY Lin KC | 2012 | Archives of Physical Medicine and Rehabilitation2012，93，3： | 1 |
| 6 | Noninvasive imaging of myocardial viability:current techniques and future developments显示文摘 | Lima JA | 2003 | Circulation2003，93，12： | 1 |
| 7 | Ultrasound guided percutaneous transhepatic fine needle asperation cytology study of gallbladder polypoid lesions显示文摘 | Lin KC Soon MS | 1996 | Am J Gastroenterol1996，91，8： | 1 |
| 8 | Genetic analysis of enterovirus 71 iso- lated from fatal and non-fatal cases of hand, foot and mouth disease during an epidemic in Taiwan, 1998 显示文摘 | Shih SR Ho MS Lin KH Wu SL Chen YT Wu CN Lin TY Chang LY Tsao KC Ning HC Chang PY Jung SM Hsueh C Chang KS | 2000 | Virus Res2000，68，2： | 1 |
| 9 | Differential effect of ECM molecules on re-expression of caailaginous markers in near quiescent human chondrocytes显示文摘 | CHIU LH CHEN SC WU KC | 2011 | J Cell Physiol2011，226，8： | 1 |
| 10 | Characterization of a specific phage-displayed peptide binding to vasculature of human gastric cancer 显示文摘 | Zhi M Wu KC Dong L | 2004 | Cancer Biol Ther2004，3，12： | 1 |
| 11 | Diabetes mellitus has an additional effect on coronary artery disease 显示文摘 | Chart KC Chou HH Wu DJ | 2004 | Jpn Heart J2004，45，6： | 1 |
| 12 | Inhibition of gastric cancer associated angiogenesis by antisense COX-2 transfectants显示文摘 | Fu YG Sung J J Wu KC | 2005 | Cancer Lett2005，224，2： | 1 |
| 13 | Additive benefits of tiotropium in COPD patients treated with long-acting beta agonists and corticosteroids 显示文摘 | Perng DW Wu CC Su KC | 2006 | Respirology2006，11，5： | 1 |
| 14 | Significance of COX-1, COX-2 and iNOS expression in superficial gastritis, gastric mucosa atypical hyperplasia and gastric carcinoma显示文摘 | Luo YQ Wu KC Sun AH Pan BR Zhang XY Fan DM | 2000 | Chin J Digestion(中华消化杂志)2000，20，4： | 1 |
| 15 | Melatonin amelio-rates high fat diet-induced diabetes and stimulates glyco-gen synthesis via a PKCzeta-Akt-GSK3beta pathway inhepatic cells显示文摘 | Shieh JM Wu HT Cheng KC | 2009 | J Pineal2009，47，4： | 1 |
| 16 | MicroRNA-224 suppresses colorectal cancer cell migration by targeting Cdc42显示文摘 | Kc TW Hsu HL Wu YH | 2014 | Dis Markers2014，，61： | 1 |
| 17 | Prognostic significance of microvascular obstruction by magnetic resonance imaging in patients with acute myocardial infarction显示文摘 | Wu KC Zerhouni EA Judd RM | 1998 | Circulation1998，97，8： | 1 |
| 18 | Transradial coronary angiography and angioplasty in chinese patients 显示文摘 | Wu C J Lo PH Chang KC | 1997 | Cathet Cardiovasc Diagn1997，40，2： | 1 |
| 19 | Cardiac magnetic resonance assessment of dyssynchrony and myocardial scar predicts function class improvement following cardiac resynchronization therapy显示文摘 | Bilchick KC Dimaano V Wu KC | 2008 | JACC Cardiovasc Imaging2008，1，5： | 1 |
| 20 | Activation of STAT3 in thymic epithelial tumours correlates with tumour type and clinical behaviour显示文摘 | Chang KC Wu MH Jones D | 2006 | J Pathol2006，210，2： | 1 |
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