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| 1 | 鸡源产气荚膜梭菌重组酶聚合酶扩增检测方法的建立显示文摘为建立鸡源产气荚膜梭菌(Clostridium perfringens)的快速诊断方法,本研究以产气荚膜梭菌α-毒素的编码基因plc为基础设计、合成引物和探针,并以real-time PCR的方法进行验证,建立了鸡源产气荚膜梭菌的重组酶聚合酶扩增(RPA)检测方法。该方法可在38℃、25 min内对产气荚膜梭菌的α-毒素编码序列进行快速特异性扩增,最低检测限量可达1×10^1copies/μL,灵敏度良好。以此方法检测实验室保存的临床样本,其检测结果与real-time PCR的检测结果一致。上述结果表明,本研究建立的real-time RPA方法在检测鸡源产气荚膜梭菌时具有操作简单、快速灵敏的特点,适用于鸡源产气荚膜梭菌的临床检测。 | 许笑 王艳华 M Umar Zafar Khan 王恒 蔡建平 | 2019 | 中国兽医科学2019，49，12： | 6 |
| 2 | Continuous wound infusion of local anaesthetic agents following colorectal surgery:Systematic review and meta-analysis显示文摘AIM:To provide a specifi c review and meta-analysis of the available evidence for continuous wound infusion of local anaesthetic agents following midline laparoto-my for major colorectal surgery. METHODS: Medline, Embase, trial registries, conference proceedings and article reference lists were searched to identify randomised, controlled trials of continuous wound infusion of local anaesthetic agents following colorectal surgery. The primary outcomes were opioid consumption, pain visual analogue scores (VASs), return to bowel function and length of hospital stay. Weighted mean difference were calculated for continuous outcomes. RESULTS: Five trials containing 542 laparotomy wounds were eligible for inclusion. There was a sig- nificant decrease in post-operative pain VAS at rest on day 3 (weighted mean difference: -0.43; 95% CI: -0.81 to -0.04; P = 0.03) but not on post-operative day 1 and 2. Local anaesthetic infusion was associated with a signifi cant reduction in pain VAS on movement on all three post-operative days (day 1 weighted mean difference: -1.14; 95% CI: -2.24 to -0.041; P = 0.04, day 2 weighted mean difference: -0.97, 95% CI: -1.91to -0.029; P = 0.04, day 3 weighted mean difference: -0.61; 95% CI: 1.01 to -0.20; P = 0.0038). Local an- aesthetic wound infusion was associated with a signifi - cant decrease in total opioid consumption (weighted mean difference: -40.13; 95% CI: -76.74 to -3.53; P = 0.03). There was no signifi cant decrease in length of stay (weighted mean difference: -20.87; 95% CI: -46.96 to 5.21; P = 0.12) or return of bowel function (weighted mean difference: -9.40; 95% CI: -33.98 to 15.17; P = 0.45). CONCLUSION: The results of this systematic re- view and meta-analysis suggest that local anaesthetic wound infusion following laparotomy for major color- ectal surgery is a promising technique but do not pro- vide conclusive evidence of benefi t. Further research is required including cost-effectiveness analysis. | Alan Karthikesalingam Stewart R Walsh Sheraz R Markar Umar Sadat Tjun Y Tang Charles M Malata | 2008 | World Journal of Gastroenterology2008，14，34： | 4 |
| 3 | Fecal microbes, short chain fatty acids, and colorectal cancer across racial/ethnic groups显示文摘AIM:To investigate differences in microbes and short chain fatty acid(SCFA) levels in stool samples from Hispanic and non-Hispanic African American,American Indian,and White participants.METHODS:Stool samples from twenty participants were subjected to analysis for relative levels of viable bacteria and for SCFA levels.Additionally,the samples were subjected to 16 S r RNA gene pyrosequencing for identification of bacteria present in the stool.We used a metagenome functional prediction technique to analyze genome copy numbers and estimate the abundance of butyrate kinase in all samples.RESULTS:We found that African Americans had significantly lower levels of acetate,butyrate,and total SCFAs than all other racial/ethnic groups.We also found that participant microbial profiles differed by racial/ethnic group.African Americans had significantly more Firmicutes than Whites,with enriched Ruminococcaceae.The Firmicutes /Bacteroidetes ratio was also significantly higher for African Americans than for Whites(P =0.049).We found Clostridium levels to be significantly and inversely related to total SCFA levels(P =0.019) and we found Bacteroides to be positively associated(P =0.027) and Clostridium to be negatively associated(P =0.012) with levels of butyrate.We also identified a correlation between copy number for a butyrate kinase predicted from 16 S r RNA gene abundance and levels of butyrate in stool.CONCLUSION:The identified differences in gut flora and SCFA levels may relate to colorectal cancer mortality differentials and may be useful as targets for future clinical and behavioral interventions. | Christina M Hester Venkatakrishna R Jala Morgan GI Langille Shahid Umar K Allen Greiner Bodduluri Haribabu | 2015 | World Journal of Gastroenterology2015，21，9： | 2 |
| 4 | What do medical students expect in a teacher?显示文摘 | Kiani Q Umar S Iqbal M | 2014 | Clin Teach2014，11，3： | 1 |
| 5 | Instructional strategy in the teaching of computer programming:a need assessment analyses显示文摘 | Ismail M N Ngah N A Umar I N | 2010 | The Turkish Online Journal of Educational Technology2010，9，2： | 1 |
| 6 | Association of commonpolymorphisms in TNFA,NFkBl and NFKBIA with risk and prog-nosis of esophageal squamous cell carcinoma显示文摘 | Umar M Upadhyay R Kumar S | 2013 | Plos One2013，8，81： | 1 |
| 7 | Pediatric pulmonary hypertension:Roles of endothelin-1 and nitric oxide显示文摘 | Black S M K umar S Wiseman D | 2007 | Clin Hemorheol Microcireulation2007，37，： | 1 |
| 8 | Biosorption of lead from aqueous so-lution by Ficus religiosa leaves:batch and column study显示文摘 | QAISER S SALEEMI A R UMAR M | 2009 | Journal ofHazardous Materials2009，166，23： | 1 |
| 9 | Kinetic studies of liquid phase ethyl tert-butyl ether (ETBE) synthesis using macroporous and gelular ion exchange resin catalysts显示文摘 | Umar M Patel D Saha B | 2009 | Chemical Enginneering Science2009，64，21： | 1 |
| 10 | Electroanalytical determination of acetaminophen using nano-TiOz/polymer coated electrode in the presence of dopamine显示文摘 | umar S A K Tang C F Chela S M | 2008 | Talanta2008，76，： | 1 |
| 11 | Hashimo- tos thyroiditis following Graves'disease 显示文摘 | UMAR H MUALLIMA N ADAM J M | 2010 | Acta Med In- dones2010，42，1： | 1 |
| 12 | Hydrogeological and hydrochemical framework of regional aquifer system in Kali-Ganga Sub-basin,India显示文摘 | Umar A Umar R Ahmad M S | 2001 | Environmental Geology2001，40，45： | 1 |
| 13 | Semigroups of weak V-atabilizer mappings显示文摘 | HIGGINS P M UMAR A | 1999 | King Fahd University of Petroleum and Mineral1999，1，： | 1 |
| 14 | Direct growth of ori- ented ZnO nanotubes by self-selective etching at lower tempera-ture for photo-electrochemical (PEC) solar cell application显示文摘 | Roza L Rahman M Y A Umar A A | 2015 | J Alloys Compd2015，618，： | 1 |
| 15 | Hashimoto's thyroiditis following Graves' disease显示文摘 | UMAR H MUALLIMA N ADAM J M | 2010 | Aeta Med Indones2010，42，1： | 1 |
| 16 | Insulin resistance and triglycer's显示文摘 | Glueck CJ Khan NA Umar M | 2009 | J Investig Med2009，57，8： | 1 |
| 17 | Local self- optimizing control of constrained processes 显示文摘 | UMAR L M XIAO G X KARIWALA V | 2012 | Jour- nal of Process Control2012，22，2： | 1 |
| 18 | Incorporating feedforward action into self-optimising control policies 显示文摘 | UMAR L M CAO Y KAEICALA V | 2014 | Canadian Journal of Chemical Engineering2014，92，1： | 1 |
| 19 | Scorpion venom(Odontobuthus doriae) induces apoptosis by depolarization of mitochondria and reduces S-phase population in human breast cancer cells(MCF-7)显示文摘 | Zargan J Umar S Sajad M | | 0，，08： | 1 |
| 20 | Patholo-gy and pathogenesis of Mycogone perniciosa infectionin Agaricus bisporus显示文摘 | Umar M H Geels F P Van Griensven | 2000 | Mushroom Science2000，，： | 1 |
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