|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Decreased phagocytic activity of Kupffer cells in a rat nonalcoholic steatohepatitis model显示文摘AIM: To investigate Kupffer cell dynamics and phagocytic activity,using a rat nonalcoholic steatohepatitis (NASH) model. METHODS: Male F344 rats were fed either a control diet or a choline-deficient L-amino acid-defined (CDAA) diet,followed by contrast enhanced ultrasonography (CEUS) using Levovist. The uptake of latex beads by the Kupffer cells was determined by fluorescent microscopy. The status of the Kupffer cells was compared between the two groups,using the immunohistochemical staining technique. RESULTS: After 4 or more wk of the CDAA diet,CEUS examination revealed a decrease in the signal intensity,20 min after intravenous Levovist. Fluorescent microscopic examination showed that the uptake of latex beads by the Kupffer cells was reduced at week 1 and 2 in the study group,compared with the controls,with no further reduction after 3 wk. Immunohistochemical staining revealed no significant difference in the Kupffer cell counts between the control group and the CDAA group. CONCLUSION: CEUS examination using Levovist demonstrated reduced contrast effect and phagocytic activity in the liver parenchymal phase,although the Kupffer cell numbers were unchanged,indicating reduced phagocytic function of the Kupffer cells in the rat NASH model. We believe that CEUS examination using Levovist is a useful screening modality,which can detect NASH in fatty liver patients. | Tatsuhiro Tsujimoto Hideto Kawaratani Toshiyuki Kitazawa Toshiko Hirai Hajime Ohishi Mitsuteru Kitade Hitoshi Yoshiji Masahito Uemura Hiroshi Fukui | 2008 | World Journal of Gastroenterology2008，14，39： | 12 |
| 2 | Innate immune reactivity of the liver in rats fed a choline-deficient L-amino-acid-defined diet显示文摘瞄准:调查肿瘤坏死 factor-alpha (TNF-alpha ) 的天生的有免疫力的反应,像使用费的受体 4 (TLR4 ) ,并且在非酒精的 steatohepatitis (NASH ) 的肝的 CD14 为老鼠建模。方法:男 F344 老鼠被喂一本胆碱缺乏的 L-amino-acid-defined (CDAA ) 食谱。老鼠在食谱的 4 或 8 wk 以后被打死,并且他们的肝为免疫被移开组织化学的调查和 RNA 抽取。肝标本是为 TNF-alpha, TLR4,和 CD14 染色的免疫。TNF-alpha, TLR4,和 CD14 的基因表情被 reverse-transcriptase 聚合酶链反应(RT-PCR ) 决定。Kupffer 房间被 Percoll 坡度远心沉淀从肝孤立,并且当时是有教养的测量 TNF-alpha 生产。结果:在老鼠作为与控制相比显著地增加了的 CDAA-fed 的 TNF-alpha 的浆液和肝层次组织,,与 steatohepatitis 的前进做了免疫组织化学的值和 TNF-alpha, TLR4,和 CD14 的基因表达式。从 CDAA-fed 老鼠的孤立的 Kupffer 房间的 TNF-alpha 生产被脂肪的多糖刺激提高。结论:TNF-alpha, TLR4,和 CD14 的表情在 NASH 模型增加了,建议 TLR4 和调停 CD14 的内毒素肝损坏可以也发生在 NASH。 | Hideto Kawaratani Tatsuhiro Tsujimoto Toshiyuki Kitazawa Mitsuteru Kitade Hitoshi Yoshiji Masahito Uemura Hiroshi Fukui | 2008 | World Journal of Gastroenterology2008，14，43： | 6 |
| 3 | Immunotherapy for nonalcoholic steatohepatitis using the multiple cytokine production modulator Y-40138显示文摘AIM:To investigate the possible use of the multiple cytokine production modulator,Y-40138,as a novel immunotherapy in the rat nonalcoholic steatohepatitis (NASH) model. METHODS:We allocated 6-wk-old male F344 rats to choline-supplemented,L-amino acid-defined (CSAA) diet (control group),CSAA diet + Y-40138 (control + Y-40138 group),choline-def icient,L-amino acid-def ined (CDAA) diet (NASH group),or CDAA diet + Y-40138 (NASH + Y-40138 group). In each group,we measured the plasma alanine aminotransferase (ALT) levels,and the plasma and liver levels of tumor necrosis factor-α (TNF-α),interferon-γ (IFN-γ),and interleukin-10 (IL-10). Tissue specimens of phosphate buffered saline-perfused liver were subjected to hematoxylin and eosin staining,Azan staining,Sirius red staining,and immunohistochemical staining (for Kupffer cells and TNF-α). We then extracted Kupffer cells from the collagenase-perfused livers using the Percoll gradient centrifugation method,and measured the TNF-α levels in the supernatant (in vitro TNF-α production by Kupffer cells) using an enzyme-linked immunosorbent assay kit.RESULTS:In comparison to the NASH group,serumALT elevation was mild,production of serum and liver TNF-α and IFN-γ was inhibited,and IL-10 production was increased in the NASH + Y-40138 group. Amelioration of liver histology was also noted in the NASH + Y-40138 group. Kupffer cell immunohistochemical staining revealed no differences between groups,whereas TNF-α immunohistochemical staining showed fewer stained cells in the NASH + Y-40138 group than in the NASH group. The TNF-α levels in the in-vitro Kupffer cell culture supernatant were lower in the NASH + Y-40138 group than in the NASH group.CONCLUSION:Administration of Y-40138 to NASH model rats reduced hepatic inflammation and suppressed fibrosis. These results indicate that the multiple cytokine production modulator,Y-40138,is promising as a novel treatment for NASH. | Tatsuhiro Tsujimoto Hideto Kawaratani Toshiyuki Kitazawa Hitoshi Yoshiji Masao Fujimoto Masahito Uemura Hiroshi Fukui | 2009 | World Journal of Gastroenterology2009，15，44： | 4 |
| 4 | Innate Immune Reactivity of the Ileum–Liver Axis in Nonalcoholic Steatohepatitis显示文摘 | Tatsuhiro Tsujimoto Hideto Kawaratani Toshiyuki Kitazawa Masahito Uemura Hiroshi Fukui | 2012 | Digestive Diseases and Sciences2012，，5： | 1 |
| 5 | Expression of Toll - like receptor 4 in various organs in rats with D- galactosamineinduced acute hepatic failure 显示文摘 | Toshiyuki Kitazawa Tatsuhiro Tsujimoto Hideto Kawaratani | 2007 | Journal of Gastroenterology and Hepatology2007，337，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员