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| 1 | Interplay of neuropilin-1 and semaphorin 3A after partial hepatectomy in rats显示文摘AIM:To elucidate the role of neuropilin-1(Nrp-1) and semaphorin 3A(Sema3A) in sinusoidal remodeling during liver regeneration in rats.METHODS:Male Wistar/ST rats at 7 wk of age,weighing about 200 g,were used for all animal experiments.In vivo,at 24,48,72,96,144 and 192 h after twothirds partial hepatectomy(PHx),the remnant livers were removed.Liver tissues were immunohistochemically stained for Nrp-1,Sema3A and SE-1,a liver sinusoidal endothelial cell(SEC) marker.Total RNA of the liver tissue was extracted and reversely transcribed into cDNA.The mRNA expression of Sema3A was analyzed by quantitative real-time polymerase chain reaction and normalized to that of ribosomal protein S18.In vitro,SECs were isolated from rat liver and cultured in endothelial growth medium containing 20 ng/mL vascular endothelial cell growth factor.Migration of SECs in primary culture was assessed by cell transwell assay with or without recombinant Sema3A.Apoptotic cells were determined by a terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling method.RESULTS:In vitro,immunohistochemistry study revealed that Sema3A and Nrp-1 were constitutively expressed in hepatocytes and SECs,respectively,in normal rat liver tissues.Nrp-1 expression in SECs was quantified by the percentage of immunostained area with antiNrp-1 antibody in relation to the area stained with SE-1.Between 24 h and 96 h following resection of liver,Nrp-1 expression in SECs was transiently increased.Compared with the baseline(5.2% ± 0.1%),Nrp-1 expression in SECs significantly increased at 24 h(17.3% ± 0.7%,P < 0.05),48 h(39.1% ± 0.6%,P < 0.01),72 h(46.9% ± 4.5%,P < 0.01) and 96 h(29.9% ± 3.8%,P < 0.01) after PHx,then returned to the basal level at termination of liver regeneration.Interestingly,the expression of Sema3A was inversely associated with that of Nrp-1 in liver after PHx.Sema3A mRNA expression was significantly reduced by about 75% over the period 24-144 h after PHx(P < 0.05),and returned to basal levels at 192 h after PHx.In vitro,SECs isolated from rats after PHx(PHx-SECs) were observed to migrate to the lower chamber of the cell transwell system after incubation for 24 h,but not cells from normal rats(CONT-SECs),indicating that mobility of PHx-SECs increases as compared with that of CONT-SECs.Moreover,recombinant Sema3A significantly attenuated migration in PHx-SECs in primary culture(vehicle-treated 100% ± 7.9% vs Sema3A-treated 42.6% ± 5.4%,P < 0.01),but not in CONT-SECs.Compared with CONTSECs,the apoptotic rate of PHx-SECs decreased by 78.3%(P < 0.05).There was no difference in apoptosis between CONT-SECs that were treated with vehicle and Sema3A.However,in PHx-SECs,apoptosis was induced by the presence of 5 nmol Sema3A for 24 h(vehicle-treated 21.7% ± 7.6% vs Sema3A-treated 104.3% ± 8.9%,P < 0.05).In addition,immunohistochemistry confirmed the increased expression of Nrp-1 in PHx-SECs,while it was noted to a lesser extent in CONT-SECs.CONCLUSION:The interplay of Nrp-1 and Sema3A shown in our results may lead to a better understanding of interaction between sinusoidal remodeling and SECs during liver regeneration. | Ling Fu Tsuneo Kitamura Kazuhisa Iwabuchi Syozo Ichinose Mitsuaki Yanagida Hideoki Ogawa Sumio Watanabe Toshihide Maruyama Masafumi Suyama Kenji Takamori | 2012 | World Journal of Gastroenterology2012，18，36： | 5 |
| 2 | 伏格列波糖抑制神经系统疾病及老年患者的餐后低血压状态显示文摘The authors examined blood pressure, glucose, insulin, and neurotensin before and after intake of 75 g glucose with or without voglibose in 28 neurologic patients and 20 healthy controls. Voglibose significantly prevented hypotension and neurotensin increment after glucose intake and had no influence on glucose or insulin increment. These results suggest that voglibose benefits postprandial hypotension. | Maruta T. Komai K. Takamori M. Yamada M. 张嘉靖(译) | 2006 | 世界核心医学期刊文摘(神经病学分册)2006，2，9： | 3 |
| 3 | Perianal tuberculosis: A case report and review of the literature显示文摘Tuberculosis(TB) is still a major health problem worldwide. We present a rare case of an immunocompetent patient with perianal TB. A 38-year-old man visited a clinic with pain, swelling, and redness in the perineum. He had been persistently coughing for the past 6 mo. The abscess had formed a fistula to the perianal region, indicating perianal abscess. Mycobacterium tuberculosis was found in sputum and perianal abscess. Surgical drainage was performed, and oral anti-tuberculous drugs were administered for 6 mo. The patient's clinical course was favorable. On review of the literature on 58 cases of perianal TB, we found that the duration of persistent perianal lesion was much longer in patients without active pulmonary TB(APTB) than in those with APTB(66.4 mo vs 8.3 mo; confidence interval, 0.0760-0.9620, P = 0.0380). Thus, in cases of non-healing or recurrent perianal lesions, TB should be considered. | Sayaka Tago Yuji Hirai Yusuke Ainoda Takahiro Fujita Mikio Takamori Ken Kikuchi | 2015 | World Journal of Clinical Cases2015，3，9： | 3 |
| 4 | Long-term outcomes of extended radical resection combined with intraoperative radiation therapy for pancreatic cancer显示文摘 | Hiroshi Takamori Takehisa Hiraoka Keiichiro Kanemitsu Tatsuya Tsuji Hiroshi Tanaka Akira Chikamoto Kei Horino Toru Beppu Masahiko Hirota Hideo Baba | 2008 | Journal of Hepato - Biliary - Pancreatic Surgery2008，，6： | 2 |
| 5 | Microstructure and mechanical properties of AZ91 alloy produced with ultrasonic vibration显示文摘 | Xinbao Liu Yoshiaki Osawa Susumu Takamori Toshiji Mukai | 2007 | Materials Science & Engineering A2007，，1： | 2 |
| 6 | Unilateral progressive multiple sclerosing hemangi-oma in a young female successfully treated by pneumonectomy:report of a case显示文摘 | Hayashi A Takamori S Mitsuoka M | 2002 | Int Surg2002，87，2： | 1 |
| 7 | Efficient cleavage of RNA, enhanced cellular uptake, and controlled intracellular localization of conjugate DNAzymes显示文摘 | Kubo T Takamori K Kanno K | 2005 | Bioorg Med Chem Lett2005，15，： | 1 |
| 8 | Local injection of hepatocyte growth factor/scatter factor (HGF/SF) alters cyclic growth of murine hair follicles 显示文摘 | Jindo T Tsuboi R Takamori K | 1998 | J lnvest Dermatol1998，110，4： | 1 |
| 9 | Receptor diseases in the field of neurology显示文摘 | Takamori M Maruta T | 2000 | Kinsho Byori2000，48，5： | 1 |
| 10 | Risk factors associated with delayed haemorrhage after pancreatic resection 显示文摘 | Nakahara O Takamori H Ikeda O | 2012 | HPB (Oxford)2012，14，10： | 1 |
| 11 | Effects of ultrasonic vibration on solidification structures of cast iron显示文摘 | Osawa Y Arakane G Takamori S etc | 1995 | J JFS1995，67，： | 1 |
| 12 | Morphology of intermetallic compounds in AI-Si-Fe alloy and its control by ultrasonic vi- bration 显示文摘 | OSAWA Y TAKAMORI S KIMURA T | 2007 | Materials Transactions2007，48，9： | 1 |
| 13 | Disruption of ClC-3,a chloride channel expressed on synaptic vesicles,leads to a loss of the hippocampus显示文摘 | Stobrawa SM Breiderhoff T Takamori S | 2001 | Neuron2001，29，1： | 1 |
| 14 | Assessment of 287Japanese cases of drug induced liver injury by the diagnostic scaleof the International Consensus Meeting 显示文摘 | Takikawa H Takamori Y Kumagi T | 2003 | Hepatol Res2003，273，： | 1 |
| 15 | An investigation of cell proliferation and soluble mediators induced by interleukin I beta in human synovial fibroblasts comparative response in osteoarthritis and rheumatoid arthritis 显示文摘 | Inouse H Takamori M Nagata N | 2001 | Inflamm Res2001，50，2： | 1 |
| 16 | Unilateral progressive multi-ple sclerosing hemangioma in a young female successfully treated by pneumonectomy : report of a case显示文摘 | Hayashi A Takamori S Mitsuoka M | 2002 | Int Surg2002，87，2： | 1 |
| 17 | An experimental study of atrial natriuretic peptide levels and the effect of inhaled nitric oxide after pneumonectomy显示文摘 | Takamori S Mifune H Tayama K | 2000 | Surg Today2000，30，： | 1 |
| 18 | Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neu-rons显示文摘 | Takamori S Rhee JS Rosenmund C | 2000 | Nature2000，407，6801： | 1 |
| 19 | Intraoperative intercostal nerve blockade for postthoracotomy pain显示文摘 | TAKAMORI S YOSHIDA S HAYASHI A | | 0，，02： | 1 |
| 20 | The modified Glasgow Prognostic Score(mGPS)is a good predictor of indication for palliative bypass surgery in patients with unresectable pancreatic and biliary cancers显示文摘 | Ikuta Y Takamori H Sakamoto Y | 2014 | Int J Clin Oncol2014，19，： | 1 |
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