|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration显示文摘AIM:To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype(SASP).METHODS:Hydrogen peroxide treatment was used to induce senescence in the human Hep G2 hepatocyte cell line.Senescence was confirmed by cytochemical staining for a panel of markers including Ki67,p21,heterochromatin protein 1β,and senescence-associated-β-galactosidase activity.Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction(q PCR),and conditioned media was used in proteomic analyses,a human chemokine protein array,and cell migration assays to characterise the composition and function of the hepatocyte SASP.RESULTS:Senescent hepatocytes induced classical markers of senescence(p21,heterochromatin protein1β,and senescence-associated-β-galactosidase activity);and downregulated the proliferation marker,Ki67.Hepatocyte senescence induced a 4.6-fold increase in total secreted protein(P=0.06)without major alterations in the protein profile.Senescence-induced genes were identified by microarray(Benjamini Hochbergcorrected P<0.05);and,consistent with the increase in secreted protein,gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes.The hepatocyte SASP included characteristic factors such as interleukin(IL)-8 and IL-6,as well as novel components such as SAA4,IL-32and Fibrinogen,which were validated by q PCR and/or chemokine protein array.Senescent hepatocyteconditioned medium elicited migration of inflammatory(granulocyte-macrophage colony stimulating factor,GM-CSF-derived),but not non-inflammatory(CSF-1-derived)human macrophages(P=0.022),which could contribute to a pro-inflammatory microenvironment in vivo,or facilitate the clearance of senescent cells.CONCLUSION:Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis. | Katharine M Irvine Richard Skoien Nilesh J Bokil Michelle Melino Gethin P Thomas Dorothy Loo Brian Gabrielli Michelle M Hill Matthew J Sweet Andrew D Clouston Elizabeth E Powell | 2014 | World Journal of Gastroenterology2014，20，47： | 2 |
| 2 | A novel pathway regulatillg lipopolysaccharide-indued shock by ST2/T1 via inhibition of loll-like receptor 4 expression显示文摘 | Sweet M J Leung BP Kang D | 2001 | J lmmunol2001，166，： | 1 |
| 3 | Very small-satellite design for distributed space missions显示文摘 | Barnhart D J Vladimirova T Sweeting M N | | 0，，06： | 1 |
| 4 | An improved method to recover saliva from human skin:the double swab technique显示文摘 | Lorente M Lorente J A | 1997 | J Forensic Sci1997，42，： | 1 |
| 5 | Effect of alkali metal impurities on the low temperature thermal stability of 2090 Al-Cu-Li alloys显示文摘 | Kerr M J Sweet E D Bennett C G | 1996 | Mater Forum1996，21，7222： | 1 |
| 6 | Modeling heterogeneity in a low-permeability gas reservoir using geostatistical techniques, Hyde field, Southern North Sea显示文摘 | SWEET M L BLEWDEN C J CARTER A M | 1996 | AAPG Bulletin1996，80，11： | 1 |
| 7 | Parkinson' s disease and megacolon: concentric hyaline inclusions(Lewy bodies) in enteric ganglion ceils显示文摘 | Kupsky W J Grimes M M Sweeting J | 1987 | Neurology1987，37，： | 1 |
| 8 | Hawthorn:pharmacology and therapeutic uses显示文摘 | RIGELSKY J M SWEET B V | 2002 | American Journal of Health-system Pharmacy2002，59，5： | 1 |
| 9 | Matrix metalloproteinase-9 (MMP-9) in the airways of infants at risk of development chronic lung disease (CLD) 显示文摘 | SWEET DG PIZZOTI J WILBOURN M | 1999 | Eur Respir J1999，14，30： | 1 |
| 10 | Middle juras-sic to lower cretaceous paleoclimate of sverdrup basin, Cana-dian arctic archipelago inferred from the palynostratigraphy显示文摘 | Galloway J M Sweet A R Swindles G T | 2013 | Marine & Petroleum Geology2013，44，3： | 1 |
| 11 | Bacterial/CpG-DNA down-modulates colony stimulating factor-1 receptor surface expression on murine bone marrow-derived macrophages with concomitant growth are stand factor-independent survival显示文摘 | Sester D P Beasley S J Sweet M J | 1999 | J Immunol1999，16，3： | 1 |
| 12 | The effects of starvation and food intake on hepatic thyroxine-monodeidinase activity in the rainbow trout, Oncorhynchus mykiss 显示文摘 | Sweeting R M Eales J G | 1992 | Can J Zool1992，70，： | 1 |
| 13 | Modeling heterogeneity in a low-permeability gas reservoir using geostatistical techniques, Hyde field, southern North Sea显示文摘 | Sweet M I tllewden C J Ceter A M | 1996 | AAPG Bulletin1996，80，11： | 1 |
| 14 | Satellite miniaturization techniques for space sensor networks显示文摘 | Barnhart D J Vladimirova T Sweeting M N | | 0，，02： | 1 |
| 15 | Anti-TNF antibody therapy in rheumatoid arthritis and the risk of serious infections and malignancies: systematic review and meta-analysis of rare harmful effects in randomized controlled trials 显示文摘 | BONGARTZ T SUTTON A J SWEETING M J | 2006 | Jama2006，295，19： | 1 |
| 16 | Prevalence of asthma symptoms among adults aged 20-44 years in Canada显示文摘 | Manfreda J Becklake MR Sears MR Chan-Yeung M Dimich-Ward H Siersted HC Ernst P Sweet L Van Til L Bowie DM Anthonisen NR Tate RB | 2001 | CMAJ2001，164，7： | 1 |
| 17 | Hawthorn: pharmocology and thera- peutic uses显示文摘 | Rigelsky J M Sweet B V | 2002 | American Journal of Health-System Pharmacy2002，59，5： | 1 |
| 18 | Endovas- cular or open repair strategy for ruptured abdominal aor- tic aneurysm: 30 day outcomes from IMPROVE rando- mized trial显示文摘 | Powell JT Sweeting M J Thompson MM | 2014 | BMJ2014，348，： | 1 |
| 19 | Influences of nickel andvanadium impurities on microstructure of aluminum alloys 显示文摘 | ZHU S M YAO J Y SWEET L | 2013 | JOM2013，65，5： | 1 |
| 20 | Review: Combination therapy with non-clozapine atypical antipsychotic medication: a review of current evidence显示文摘 | Chan J Sweeting M | 2007 | J Psychopharmacol2007，21，6： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员