| 1 | SmMYB2 promotes salvianolic acid biosynthesis in the medicinal herb Salvia miltiorrhiza显示文摘MYB transcription factors play vital roles in plant growth and metabolism.The phytohormone methyl jasmonate(MeJA)promotes phenolic acid accumulation in the medicinal herb Salvia miltiorrhiza,but the regulatory mechanism is poorly understood.Here,we identified the MeJA-responsive R2R3-MYB transcription factor gene 5mMYB2 from a transcriptome library produced from MeJAtreated S.mi'/horr/ifza hairy roots.Sm/VIYB2 expression was tightly correlated with the expression of key salvianolic acid biosynthetic genes including CYP^8Ai^.5mMYB2 was highly expressed in the periderm of S.miltiorrhiza and SmMYB2 localized to the nucleus.Overexpressing Sml\AYB2 in S.miltiorrhiza hairy roots significantly increased the levels of salvianolic acids(including rosmarinic acid and salvianolic acid B)by upregulating salvianolic acid biosynthetic genes such as CYP98A14.SmMYB2 binds to the MYB-binding motifs in the promoter of CYP98A14,as confirmed by a dual-luciferase assay and electrophoretic mobility shift assays.Anthocyanin contents were significantly higher in Sm/ViyB2-overexpressing hairy root lines than the control,primarily due to the increased expression of CHI,DFR,and ANS.These findings reveal the novel regulatory role of SmMYB2 in MeJA-mediated phenolic acid biosynthesis,providing a useful target gene for metabolic engineering and shedding light on the salvianolic acid regulatory network. | Changping Deng Yao Wang Fenfen Huang Sunjie Lu Limei Zhao Xingyuan Ma Guoyin Kai | 2020 | Journal of Integrative Plant Biology2020，62，11： | 6 |
| 2 | The methyl jasmonate-responsive transcription factor SmMYB1 promotes phenolic acid biosynthesis in Salvia miltiorrhiza显示文摘Water-soluble phenolic acids are major bioactive compounds in the medicinal plant species Salvia miltiorrhiza.Phenolic acid biosynthesis is induced by methyl jasmonate(MeJA)in this important Chinese herb.Here,we investigated the mechanism underlying this induction by analyzing a transcriptome library of S.miltiorrhiza in response to MeJA.Global transcriptome analysis identified the MeJA-responsive R2R3-MYB transcription factor-encoding gene SmMYB1.Overexpressing SmMYB1 significantly promoted phenolic acid accumulation and upregulated the expression of genes encoding key enzymes in the phenolic acid biosynthesis pathway,including cytochrome P450-dependent monooxygenase(CYP98A14).Dual-luciferase(dual-LUC)assays and/or an electrophoretic mobility shift assays(EMSAs)indicated that SmMYB1 activated the expression of CYP98A14,as well as the expression of genes encoding anthocyanin biosynthesis pathway enzymes,including chalcone isomerase(CHI)and anthocyanidin synthase(ANS).In addition,SmMYB1 was shown to interact with SmMYC2 to additively promote CYP98A14 expression compared to the action of SmMYB1 alone.Taken together,these results demonstrate that SmMYB1 is an activator that improves the accumulation of phenolic acids and anthocyanins in S.miltiorrhiza.These findings lay the foundation for in-depth studies of the molecular mechanism underlying MeJA-mediated phenolic acid biosynthesis and for the metabolic engineering of bioactive ingredients in S.miltiorrhiza. | Wei Zhou Min Shi Changping Deng Sunjie Lu Fenfen Huang Yao Wang Guoyin Kai | 2021 | Horticulture Research2021，8，1： | 3 |
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