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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | Dissolved inorganic carbon and its isotopic differentiation in cascade reservoirs in the Wujiang drainage basin显示文摘Three cascade reservoirs,built in different periods of time in the Wujiang drainage basin,were inves-tigated in this study.Samples were taken at the surface and also at 20,40,60,80 m depths in front of the dams in April,July,October of 2006 and January of 2007.Chemical parameters were calculated and the concentrations of dissolved inorganic carbon DIC and its isotopic composition(δ13CDIC) were de-termined.In surface waters,the δ13CDIC values are high in summer and autumn and low in winter and spring,while the DIC concentrations are relatively low in summer and autumn and relatively high in winter and spring.In the water column,the DIC concentrations increase while δ13CDIC values decrease with water depth.DIC in various reservoirs is significantly different in isotopic composition from that in natural rivers,but is close to that in natural lakes.In addition,in surface waters,the δ13CDIC values tend to become lower whereas the nutrition level tends to become higher with increasing age of the reser-voirs.The conclusion is that after dam blocking,changes took place in the hydrochemical properties of river water,and the impounding rivers developed toward lakes and swamps.In addition,differentiation in DIC isotopic composition may be used to some extent to trace the evolution process of a reservoir. | YU YuanXiu LIU CongQiang WANG FuShun WANG BaoLi LI Jun LI SiLiang | 2008 | Chinese Science Bulletin2008，53，21： | 9 |
| 3 | Heterogeneous lamellar-edged Fe-Ni(OH)_(2)/Ni_(3)S_(2)nanoarray for efficient and stable seawater oxidation显示文摘Development of efficient non-precious catalysts for seawater electrolysis is of great significance but challenging due to the sluggish kinetics of oxygen evolution reaction(OER)and the impairment of chlorine electrochemistry at anode.Herein,we report a heterostructure of Ni_(3)S_(2)nanoarray with secondary Fe-Ni(OH)_(2)lamellar edges that exposes abundant active sites towards seawater oxidation.The resultant Fe-Ni(OH)_(2)/Ni_(3)S_(2)nanoarray works directly as a free-standing anodic electrode in alkaline artificial seawater.It only requires an overpotential of 269 mV to afford a current density of 10 mA·cm^(-2)and the Tafel slope is as low as 46 m V·dec^(-1).The 27-hour chronopotentiometry operated at high current density of 100 mA·cm^(-2)shows negligible deterioration,suggesting good stability of the Fe-Ni(OH)_(2)/Ni_(3)S_(2)@NF electrode.Faraday efficiency for oxygen evolution is up to〜95%,revealing decent selectivity of the catalyst in saline water.Such desirable catalytic performance could be benefitted from the introduction of Fe activator and the heterostructure that offers massive active and selective sites.The density functional theory(DFT)calculations indicate that the OER has lower theoretical overpotential than Cl_(2) evolution reaction in Fe sites,which is contrary to that of Ni sites.The experimental and theoretical study provides a strong support for the rational design of high-performance Fe-based electrodes for industrial seawater electrolysis. | Baihua Cui Zheng Hu Chang Liu Siliang Liu Fangshuai Chen Shi Hu Jinfeng Zhang Wei Zhou Yida Deng Zhenbo Qin Zhong Wu Yanan Chen Lifeng Cui Wenbin Hu | 2021 | Nano Research2021，14，4： | 7 |
| 4 | Nitrous oxide (N_2O) emissions from a mesotrophic reservoir on the Wujiang River,southwest China显示文摘Aquatic ecosystems have been identified as a globally significant source of nitrous oxide(N_2O) due to continuous active nitrogen involvement, but the processes and influencing factors that control N_2O production are still poorly understood, especially in reservoirs. For that, monthly N_2O variations were monitored in Dongfeng reservoir(DFR)with a mesotrophic condition. The dissolved N_2O concentration in DFR displayed a distinct spatial–temporal pattern but lower than that in the eutrophic reservoirs. During the whole sampling year, N_2O saturation ranging from 144% to 640%, indicating that reservoir acted as source of atmospheric N_2O. N_2O production is induced by the introduction of nitrogen(NO_3^-, NH_4^+) in mesotrophic reservoirs, and is also affected by oxygen level and water temperature. Nitrification was the predominate process for N_2O production in DFR due to well-oxygenated longitudinal water layers.Mean values of estimated N_2O flux from the air–water interface averaged 0.19 μmol m^(-2)h^(-1) with a range of 0.01–0.61 μmol m^(-2)h^(-1). DFR exhibited less N_2O emission flux than that reported in a nearby eutrophic reservoir, but still acted as a moderate N_2O source compared with other reservoirs and lakes worldwide. Annual emissions from the water–air interface of DFR were estimated to be 0.32×10~5 mol N–N_2O, while N_2O degassing from releasing water behind the dam during power generation was nearly five times greater. Hence, N_2O degassing behind the dam should be taken into account for estimation of N_2O emissions from artificial reservoirs, an omission that historically has probably resulted in underestimates. IPCC methodology should consider more specifically N_2O emission estimation in aquatic ecosystems, especially in reservoirs, the default EF5 model will lead to an overestimation. | Xiaolong Liu Siliang Li Zhongliang Wang Guilin Han Jun Li Baoli Wang Fushun Wang Li Bai | 2017 | Acta Geochimica2017，36，4： | 6 |
| 5 | Plastic wrinkling prediction in thin-walled part forming process: A review显示文摘The precision forming of thin-walled components has been urgently needed in aviation and aerospace field. However, the wrinkling induced by the compressive instability is one of the major defects in thin-walled part forming. The initiation and growth of the wrinkles are interactively affected by many factors such as stress states, mechanical properties of the material, geometry of the workpiece and boundary conditions. Especially when the forming process involves complicated boundary conditions such as multi-dies constrains, the perturbation of clearances between workpiece and dies and the contact conditions changing in time and space, etc., the predication of the wrinkling is further complicated. In this paper, the current prediction methods were summarized including the static equilibrium method, the energy method, the initial imperfection method,the eigenvalue buckling analysis method, the static-implicit finite element method and the dynamicexplicit finite element method. Then, a systematical comparison and summary of these methods in terms of their advantages and limitations are presented. By using a combination of explicit FE method, initial imperfection and energy conservation, a hybrid method is recommended to predict plastic wrinkling in thin-walled part forming. Finally, considering the urgent requirements of complex thin-walled structures' part in aviation and aerospace field, the trends and challenges in wrinkling prediction under complicated boundary conditions are presented. | Liu Nan Yang He Li Heng Yan Siliang | 2016 | Chinese Journal of Aeronautics2016，29，1： | 5 |
| 6 | Sulfuric acid as a weathering agent of carbonate weathering constrained by δ^(13)C: Examples from Southwest China显示文摘 | Siliang LI Jérome Gaillardet Guilin HAN Damien Calmels Congqiang LIU | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 4 |
| 7 | Thinking on the informatization development of China’s healthcare system in the post-COVID-19 era显示文摘With the application of Internet of Things,big data,cloud computing,artificial intelligence,and other cuttingedge technologies,China’s medical informatization is developing rapidly.In this paper,we summaried the role of information technology in healthcare sector’s battle against the coronavirus disease 2019(COVID-19)from the perspectives of early warning and monitoring,screening and diagnosis,medical treatment and scientific research,analyzes the bottlenecks of the development of information technology in the post-COVID-19 era,and puts forward feasible suggestions for further promoting the construction of medical informatization from the perspectives of sharing,convenience,and safety. | Ming Zhang Danyun Dai Siliang Hou Wei Liu Feng Gao Dong Xu Yu Hu | 2021 | Intelligent Medicine2021，1，1： | 4 |
| 8 | Acquisition algorithm assisted by AGC control voltage for DSSS signals显示文摘An appropriate acquisition configuration in terms of signal quality can optimize the acquisition performance. In view of this, a new approach of acquisition assisted by the control voltage of automatic gain control(AGC) is proposed. This approach judges the signal power according to the AGC control voltage and switches the working modes correspondingly and adaptively. Non-coherent accumulation times and the detection threshold are reconfigured according to the working mode. Theoretical derivation and verification by simulation in typical situations are provided, and the algorithm is shown to be superior in terms of the mean acquisition time, especially in strong signal scenarios compared with the conventional algorithm. | SHEN YuYao WANG YongQing LIU MinLi WU SiLiang | 2015 | Science China(Technological Sciences)2015，58，12： | 4 |
| 9 | Real-Time Implementation for Reduced-Complexity LDPC Decoder in Satellite Communication显示文摘In this paper,it has proposed a realtime implementation of low-density paritycheck(LDPC) decoder with less complexity used for satellite communication on FPGA platform.By adopting a(2048.4096)irregular quasi-cyclic(QC) LDPC code,the proposed partly parallel decoding structure balances the complexity between the check node unit(CNU) and the variable node unit(VNU) based on min-sum(MS) algorithm,thereby achieving less Slice resources and superior clock performance.Moreover,as a lookup table(LUT) is utilized in this paper to search the node message stored in timeshare memory unit,it is simple to reuse and save large amount of storage resources.The implementation results on Xilinx FPGA chip illustrate that,compared with conventional structure,the proposed scheme can achieve at last 28.6%and 8%cost reduction in RAM and Slice respectively.The clock frequency is also increased to 280 MHz without decoding performance deterioration and convergence speed reduction. | WANG Yongqing LIU Donglei SUN Lida WU Siliang | 2014 | China Communications2014，11，12： | 4 |
| 10 | An SEU-Tolerant Approach for Space-Borne Viterbi Decoders显示文摘In the space environment, Viterbi decoder implemented on SRAM-based FPGA is sensitive to Single event upsets(SEUs), which may lead to functional failure of the decoder. Conventional SEU mitigation techniques like modular redundancy could not exploit the characters of Viterbi decoders,therefore could not provide optimized SEU tolerance when the device resource utilization cost is a constraint. Leveraging the properties of the decoding algorithm, three effective mitigation techniques are adopted, including structure optimization, Error detection and correction(EDAC) for Block RAM(BRAM) protection, and Partial triple-modular redundancy(PTMR), which are applied to the modules of the decoder in accordance with their characteristics. Analysis of effectiveness shows that compared with unmitigated design, the SEU induced failure rate in the proposed SEU tolerant decoder can be reduced to 1/4 at the cost of 61.1% extra resource utilization. | WANG Yongqing MA Yuanxing LIU Donglei WU Siliang | 2014 | Chinese Journal of Electronics2014，23，4： | 3 |
| 11 | Optimal Day-ahead Scheduling of Islanded Microgrid Considering Risk-based Reserve Decision显示文摘Due to the lack of support from the main grid,the intermittency of renewable energy sources(RESs)and the fluctuation of load will derive uncertainties to the operation of islanded microgrids(IMGs).It is crucial to allocate appropriate reserve capacity for the economic and reliable operation of IMGs.With the high penetration of RESs,it faces both economic and environmental challenges if we only use spinning reserve for reserve support.To solve these problems,a multi-type reserve scheme for IMGs is proposed according to different operation characteristics of generation,load,and storage.The operation risk due to reserve shortage is modeled by the conditional value-at-risk(CVaR)method.The correlation of input variables is considered for the forecasting error modeling of RES and load,and Latin hypercube sampling(LHS)is adopted to generate the random scenarios of the forecasting error,so as to avoid the dimension disaster caused by conventional large-scale scenario sampling approaches.Furthermore,an optimal day-ahead scheduling model of joint energy and reserve considering riskbased reserve decision is established to coordinate the security and economy of the operation of IMGs.Finally,the comparison of numerical results of different schemes demonstrate the rationality and effectiveness of the proposed scheme and model. | Zehuai Liu Siliang Liu Qinhao Li Yongjun Zhang Wenyang Deng Lai Zhou | 2021 | Journal of Modern Power Systems and Clean Energy2021，9，5： | 2 |
| 12 | Using the dual isotopes approach to identify the nitrate sources of karst groundwater, Guiyang, Southwest China.显示文摘 | Siliang LI Congqiang LIU Yunchao LANG | 2006 | Chinese Journal Of Geochemistry2006，25，B08： | 2 |
| 13 | Nanobody-based chimeric antigen receptor T cells designed by CRISPR/Cas9 technology for solid tumor immunotherapy显示文摘Chimeric antigen receptor-based T-cell immunotherapy is a promising strategy for treatment of hematological malignant tumors;however,its efficacy towards solid cancer remains challenging.We therefore focused on developing nanobody-based CAR-T cells that treat the solid tumor.CD105 expression is upregulated on neoangiogenic endothelial and cancer cells.CD105 has been developed as a drug target.Here we show the generation of a CD105-specific nanobody,an anti-human CD105 CAR-T cells,by inserting the sequences for anti-CD105 nanobody-linked standard cassette genes into AAVS1 site using CRISPR/Cas9 technology.Co-culture with CD105+target cells led to the activation of anti-CD105 CAR-T cells that displayed the typically activated cytotoxic T-cell characters,ability to proliferate,the production of pro-inflammatory cytokines,and the specific killing efficacy against CD105+target cells in vitro.The in vivo treatment with anti-CD105 CAR-T cells significantly inhibited the growth of implanted CD105+tumors,reduced tumor weight,and prolonged the survival time of tumor-bearing NOD/SCID mice.Nanobody-based CAR-T cells can therefore function as an antitumor agent in human tumor xenograft models.Our findings determined that the strategy of nanobody-based CAR-T cells engineered by CRISPR/Cas9 system has a certain potential to treat solid tumor through targeting CD105 antigen. | Fengzhen Mo Siliang Duan Xiaobing Jiang Xiaomei Yang Xiaoqiong Hou Wei Shi Cueva Jumbo Juan Carlos Aiqun Liu Shihua Yin Wu Wang Hua Yao Zihang Yu Zhuoran Tang Shenxia Xie Ziqiang Ding Xinyue Zhao Bruce D.Hammock Xiaoling Lu | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 2 |
| 14 | Spatial and temporal variation of global LAI during 1981-2006显示文摘Earth is always changing.Knowledge about where changes happened is the first step for us to understand how these changes affect our lives.In this paper,we use a long-term leaf area index data(LAI) to identify where changes happened and where has experienced the strongest change around the globe during 1981-2006.Results show that,over the past 26 years,LAI has generally increased at a rate of 0.0013 per year around the globe.The strongest increasing trend is around 0.0032 per year in the middle and northern high latitudes(north of 30°N).LAI has prominently increased in Europe,Siberia,Indian Peninsula,America and south Canada,South region of Sahara,southwest corner of Australia and Kgalagadi Basin;while noticeably decreased in Southeast Asia,southeastern China,central Africa,central and southern South America and arctic areas in North America. | LIU Siliang LIU Ronggao LIU Yang | 2010 | Journal of Geographical Sciences2010，20，3： | 2 |
| 15 | Risk of tuberculosis in patients with rheumatoid arthritis treated with biological and targeted drugs:meta-analysis of randomized clinical trials显示文摘Background:Concerns exist regarding the potential development of tuberculosis in patients with rheumatoid arthritis(RA)treated with biological and targeted drugs.We assessed systematically whether biological therapy increased the risk of tuberculosis in patients with RA by meta-analysis of randomized controlled trials(RCTs).Methods:A systematic literature search was conducted in PubMed,Embase,the Cochrane Library,and China Biology Medicine disc for RCTs evaluating biological therapy in patients with RA from inception through August 2021.Traditional meta-analysis and network meta-analysis were performed to compare the risk of tuberculosis for each biologics class in patients with RA.Peto odds ratio(Peto OR)and its 95%confidence interval(CI)were calculated as the primary effect measure.Results:In total,39 studies with 20,354 patients were included in this meta-analysis,and 82 patients developed tuberculosis.The risk of tuberculosis was increased in patients treated with biologics compared with non-biologics(Peto OR:3.86,95%CI:2.36-6.32,P<0.001).Also,tumor necrosis factor-α(TNF-α)inhibitors had a higher probability of developing tuberculosis than placebo(Peto OR:3.98,95%CI:2.30-6.88,P<0.001).However,network meta-analysis demonstrated that there was no significant difference in the risk of tuberculosis for each biologics class in patients with RA.Noticeably,tuberculosis was significantly more common in patients treated with a high dose compared with patients receiving a low dose of tofacitinib(Peto OR:7.39,95%CI:2.00-27.31,P=0.003).Conclusion:This meta-analysis demonstrates the evidence of an elevated risk of tuberculosis in patients with RA treated with TNF-αinhibitors,and a dose-dependent elevated risk of tuberculosis in patients treated with tofacitinib. | Xiaojian Ji Lidong Hu Yiwen Wang Siliang Man Xingkang Liu Chuan Song Jiaxin Zhang Jian Zhu Jianglin Zhang Feng Huang | 2022 | Chinese Medical Journal2022，，4： | 2 |
| 16 | Sulfate sources constrained by sulfur and oxygen isotopic compositions in the upper reaches of the Xijiang River,China显示文摘While it is critical to accurately understand the sources and transformation of sulfate based on time-series analysis, there are limited studies on temporal variation of sulfate in rivers and on rock weathering by sulfuric acids.We conducted a monthly sampling campaign in the Beipan, Nanpan, and Hongshui Rivers over the course of one hydrological year. This study examined seasonal variations in riverine sulfate impacted by the monsoon climate in the upper reaches of the Xijiang River basin. In general, the SO_4^(2-) contents in these rivers dropped from relatively high levels to low values during the high-flow season, in response to increasing discharge. The sulfate was generally enriched in heavy isotopes during the low-flow season compared to the high-flow season. The calculated results indicate that the riverine sulfate was mainly derived from sulfide oxidation, but that evaporite dissolution could be an important source during the low-flow season, based on isotopic evidence. Mine drainage is likely an important source of sulfate to these rivers during the high-flow season due to contributions from fast surface flow, which responds to frequent heavy rain in monsoonal climate regions. Arelatively high proportion of HCO_3^- was found to be derived from rock weathering by sulfuric acid during the high-flow season when compared to that observed during the low-flow season. The results suggest that approximately one quarter of the HCO_3^- in the Hongshui River originated from carbonate weathering by sulfuric acid derived from the oxidation of sulfide. Such information on the specific dual isotopic characteristics of riverine sulfate throughout a hydrological year can provide unique evidence for understanding the temporal variability of sulfate concentrations and weathering processes in rivers. | Jing Liu Siliang Li Jun Zhong Xuetao Zhu Qingjun Guo Yunchao Lang Xiaokun Han | 2017 | Acta Geochimica2017，36，4： | 2 |
| 17 | Assessment of the sources of nitrate in the Changjiang River, China using a nitrogen and oxygen isotopic approach 显示文摘 | Li Siliang Liu Congqiang Li Jun | 2010 | Environmental Science and Technology2010，44，5： | 1 |
| 18 | Evaluation of nitrate source in surface water of southwestern China based on stable isotopes显示文摘 | LI Siliang LIU Congqiang LI Jun | 2013 | Environmental Earth Sciences2013，68，1： | 1 |
| 19 | An edge detection approach based on directional wavelet transform显示文摘 | Zhen Zhang Siliang Ma Hui Liu Yuexin Gong | 2009 | Computers and Mathematics with Applications2009，，8： | 1 |
| 20 | A natural visible and infrared facial expression database for expression recognition and emotion inference显示文摘 | Wang Shangfei Liu Zhilei Lyu Siliang | 2010 | IEEE Trans on Multimedia2010，12，7： | 1 |
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