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| 1 | Personalized treatment based on mini patient-derived xenografts and WES/RNA sequencing in a patient with metastatic duodenal adenocarcinoma显示文摘Background:Treatment guidelines for a variety of cancers have been increasingly used in clinical practice,and have resulted in major improvement in patient outcomes.However,recommended regimens(even first-line treat-ments)are clearly not ideal for every patients.In the present study,we used mini patient-derived xenograft(mini-PDX)and next-generation sequencing to develop personalized treatment in a patient with metastatic duodenal adenocarcinoma.Methods:Resected metachronous metastatic tumor tissues were implanted into SCID mice to determine the sensitivity to a variety of drug regimens.Mutation profiles were assessed using both DNA whole-exome sequencing(DNA-WES)and RNA sequencing.The results of the analyses were used to select optimal treatment for the patient with metastatic duodenal adenocarcinoma.Results:Assessment with mini-PDX models took only 7 days.The results showed high sensitivity to S-1 plus cis-platin,gemcitabine plus cisplatin and everolimus alone.The patient received gemcitabine plus cisplatin initially,but the treatment was terminated due to toxicity.The patient was then switched to treatment with S-1 alone.The overall disease-free survival was 34 months.DNA-WES and RNA sequencing identified KRAS mutation(A146T),TP53(C229Yfs*10)and RICTOR amplification in the metastatic duodenal adenocarcinoma.These findings provided further support to the results of the mini-PDX,and suggest mTOR inhibitors should be used if and when relapse eventually occurs in this patient.Conclusions:Mini-PDX model combined with WES/RNA sequencing can rapidly assess drug sensitivity in cancer patients and reveal key genetic alterations.Further research on this technology for personalized therapy in patients with refractory malignant tumors is warranted. | Peng Zhao Hui Chen Danyi Wen Shuo Mou Feifei Zhang Shusen Zheng | 2018 | Cancer Communications2018，38，1： | 11 |
| 2 | Differential gene expression in proximal and distal nerve segments of rats with sciatic nerve injury during Wallerian degeneration显示文摘Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration: transcription factor activation, immune response, myelin cell differentiation and dedifferentiation. Although gene expression responses in the distal segment of the sciatic nerve after peripheral nerve injury are known, differences in gene expression between the proximal and distal segments remain unclear. In the present study in rats, we used microarrays to analyze changes in gene expression, biological processes and signaling pathways in the proximal and distal segments of sciatic nerves undergoing Wallerian degeneration. More than 6,000 genes were differentially expressed and 20 types of expression tendencies were identified, mainly between proximal and distal segments at 7–14 days after injury. The differentially expressed genes were those involved in cell differentiation, cytokinesis, neuron differentiation, nerve development and axon regeneration. Furthermore, 11 biological processes were represented, related to responses to stimuli, cell apoptosis, inflammatory response, immune response, signal transduction, protein kinase activity, and cell proliferation. Using real-time quantitative PCR, western blot analysis and immunohistochemistry, microarray data were verified for four genes: aquaporin-4, interleukin 1 receptor-like 1, matrix metalloproteinase-12 and periaxin. Our study identifies differential gene expression in the proximal and distal segments of a nerve during Wallerian degeneration, analyzes dynamic biological changes of these genes, and provides a useful platform for the detailed study of nerve injury and repair during Wallerian degeneration. | Nan Jiang Huaiqin Li Yi Sun Dexin Yin Qin Zhao Shusen Cui Dengbing Yao | 2014 | Neural Regeneration Research2014，9，12： | 5 |
| 3 | Preliminary study on climatic signals of stable isotopes from Holocene speleothems under monsoon condition显示文摘Within the East Asian monsoon zone the change in oxygen isotope of Holocene stalagmite is mainly related to monsoon rain rather than to temperature. And carbon isotope can be taken as a comprehensive signal which contains the natural information and the information of anthropogenic activity. Isotopic analysis is also compared with the annual layer records. | Ming Tan Dongsheng Liu Hua Zhong Xiaoguang Qin Hongchun Li Shusen Zhao Tieying Li Jinbo Lu Xiangyang Lu | 1998 | Chinese Science Bulletin1998，43，6： | 4 |
| 4 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 5 | Mathematical simulation of hot metal desulfurization during KR process coupled with an unreacted core model显示文摘A three-dimensional mathematical model was established to predict the multiphase flow,motion and dispersion of desulfurizer particles,and desulfurization of hot metal during the Kanbara reactor(KR)process.The turbulent kinetic energy-turbulent dissipation rate(k-ε)turbulence model,volume-of-fluid multiphase model,discrete-phase model,and unreacted core model for the reaction between the hot metal and particles were coupled.The measured sulfur content of the hot metal with time during the actual KR process was employed to validate the current mathematical model.The distance from the lowest point of the liquid level to the bottom of the ladle decreased from 3170 to2191 mm when the rotation speed increased from 30 to 110 r/min,which had a great effect on the dispersion of desulfurizer particles.The critical rotation speed for the vortex to reach the upper edge of the stirring impeller was 70 r/min when the immersion depth was 1500 mm.The desulfurization rate increased with the increase in the impeller rotation speed,whereas the influence of the immersion depth was relatively small.Formulas for different rotation parameters on the desulfurization rate constant and turbulent energy dissipation rate were proposed to evaluate the variation in sulfur content over time. | Yanyu Zhao Wei Chen Shusen Cheng Lifeng Zhang | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，4： | 2 |
| 6 | Influence of Pre-Compressive Creep on Internal Friction Stress and Creep Parameters of Nickel-Base Single Crystal Superalloys显示文摘By means of pre-compressive creep treatment, the cubic γ′ phase in a nickel base single crystal superalloy is transformed into the P-type rafted structure. And the influence of the pre-compressive creep on the internal friction stress and creep lifetimes of the superalloy are investigated by means of the measurement of the creep curves and microstructure observation. Results show that, compared to the P-type structure alloy, the full heat treated state alloy displays a bigger internal friction stress value of dislocation motion during steady state creep and a longer creep lifetimes. The creep activation energies of the full heat treated and P-type structures alloys are measured to be 462 kJ/mol and 412 kJ/mol, respectively. Thereinto, the P-type rafted γ′ phase in the alloy is transformed into the N-type structure during tensile creep. And the N-type γ′ phase transformed from the P-type structure displays a shorter size in length, this is a main reason of the P-type structure alloy possessing a shorter creep lifetimes due to creep dislocation moving easily over the rafted γ′ phase. | Yu Xingfu Tian Sugui Zhao Zhonggang Du Hongqiang Shang Lijuan Cui Shusen | 2009 | 稀有金属材料与工程2009，38，A03： | 2 |
| 7 | Optimization for the structure of BF hearth bottom and the arrangement of thermal couples显示文摘The hearth of “heat transfer method” and the ceramic cup synthetic hearth bottom of “heart isolation method” are two most popular designs for blast furnace (BF). Although there are successful real examples, some disadvantages, for instance large heat loss and high cost, still exist for these designs. According to the theory of heat transfer, based on the calculation of temperature distribution of the hearth bottom, it is elucidated that all brick layers at the hearth bottom may not be considered as the only reason why different structures exhibited different temperature distributions although total heat resistance is the same, and then based on the effect of hot metal and cold water on different temperature distribution ranges, the concepts of “heat resistance” and “cooling enhancement” are put forth. Based on this, the disadvantages and the factors affecting temperature distribution, of the two types of hearth bottoms were illustrated. On the basis of these analyses, a novel structure for BF hearth bottom designing that can easily form “self-protecting” slag layer stably, called “the method of gradient brick layout that has an optimum combination of cooling enhancement and heat resistance” was proposed; it can not only prolong the hearth bottom longevity but also reduce the cost and heat loss. Also, the optimum arrangement of thermal couples in hearth bottom was suggested based on the previous studies on erosion prediction carried out by the author. | ZHAO Hongbo CHENG Shusen | 2006 | 北京科技大学学报2006，28，12： | 2 |
| 8 | Biomarkers of aging显示文摘Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly characterized.An immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this need.Here,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory factors.To fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant. | Aging Biomarker Consortium Hainan Bao Jiani Cao Mengting Chen Min Chen Wei Chen Xiao Chen Yanhao Chen Yu Chen Yutian Chen Zhiyang Chen Jagadish K Chhetri Yingjie Ding Junlin Feng Jun Guo Mengmeng Guo Chuting He Yujuan Jia Haiping Jiang Ying Jing Dingfeng Li Jiaming Li Jingyi Li Qinhao Liang Rui Liang Feng Liu Xiaoqian Liu Zuojun Liu Oscar Junhong Luo Jianwei Lv Jingyi Ma Kehang Mao Jiawei Nie Xinhua Qiao Xinpei Sun Xiaoqiang Tang Jianfang Wang Qiaoran Wang Siyuan Wang Xuan Wang Yaning Wang Yuhan Wang Rimo Wu Kai Xia Fu-Hui Xiao Lingyan Xu Yingying Xu Haoteng Yan Liang Yang Ruici Yang Yuanxin Yang Yilin Ying Le Zhang Weiwei Zhang Wenwan Zhang Xing Zhang Zhuo Zhang Min Zhou Rui Zhou Qingchen Zhu Zhengmao Zhu Feng Cao Zhongwei Cao Piu Chan Chang Chen Guobing Chen Hou-Zao Chen Jun Chen Weimin Ci Bi-Sen Ding Qiurong Ding Feng Gao Jing-Dong JHan Kai Huang Zhenyu Ju Qing-Peng Kong Ji Li Jian Li Xin Li Baohua Liu Feng Liu Lin Liu Qiang Liu Qiang Liu Xingguo Liu Yong Liu Xianghang Luo Shuai Ma Xinran Ma Zhiyong Mao Jing Nie Yaojin Peng Jing Qu Jie Ren Ruibao Ren Moshi Song Zhou Songyang Yi Eve Sun Yu Sun Mei Tian Shusen Wang Si Wang Xia Wang Xiaoning Wang Yan-Jiang Wang Yunfang Wang Catherine CL Wong Andy Peng Xiang Yichuan Xiao Zhengwei Xie Daichao Xu Jing Ye Rui Yue Cuntai Zhang Hongbo Zhang Liang Zhang Weiqi Zhang Yong Zhang Yun-Wu Zhang Zhuohua Zhang Tongbiao Zhao Yuzheng Zhao Dahai Zhu Weiguo Zou Gang Pei Guang-Hui Liu | 2023 | Science China(Life Sciences)2023，66，5： | 2 |
| 9 | Microstructure and properties of laser cladding FeCrBSi composite powder coatings with higher Cr content 显示文摘 | Yibo Wang Shusen Zhao Wenyan Gao | 2014 | Journal of Materials Processing Technology2014，214，4： | 1 |
| 10 | The enhancement of henzotriazole on epoxy functionalieed silica sol-gel coating for copper protection 显示文摘 | Peng Shusen Zhao Wenjie Li He | 2013 | Applied Surface Science2013，276，： | 1 |
| 11 | Study on the deterioration process of a chromium-free conversion coating on AZ91D magnesium alloy in NaC1 solution显示文摘 | Zhao Ming Wu Shusen | 2006 | Appl Surf Sei2006，253，2： | 1 |
| 12 | Micro- structural and mechanical characteristics of laser weld- ing of Ti6Al4V and lead metal 显示文摘 | ZHAO SHUSEN YU GANG HE XINLI | 2012 | Journal of Materials Processing Technology2012，212，7： | 1 |
| 13 | The coupling effect of slow-rate mechanical motion on the confined etching process in electrochemical mechanical micromachining显示文摘By introducing the mechanical motion into the confined etchant layer technique(CELT), we have developed a promising ultraprecision machining method, termed as electrochemical mechanical micromachining(ECMM), for producing both regular and irregular three dimensional(3 D) microstructures. It was found that there was a dramatic coupling effect between the confined etching process and the slow-rate mechanical motion because of the concentration distribution of electrogenerated etchant caused by the latter. In this article, the coupling effect was investigated systemically by comparing the etchant diffusion, etching depths and profiles in the non-confined and confined machining modes. A two-dimensional(2 D) numerical simulation model was proposed to analyze the diffusion variations during the ECMM process, which is well verified by the machining experiments. The results showed that, in the confined machining mode, both the machining resolution and the perpendicularity tolerance of side faces were improved effectively. Furthermore, the theoretical modeling and numerical simulations were proved valuable to optimize the technical parameters of the ECMM process. | Lianhuan Han Yuchao Jia Yongzhi Cao Zhenjiang Hu Xuesen Zhao Shusen Guo Yongda Yan Zhongqun Tian Dongping Zhan | 2018 | Science China Chemistry2018，61，6： | 1 |
| 14 | A chromium-free conversion coating of magnesium alloy by a phosphate-permanganate solution 显示文摘 | Ming Zhao Shusen Wu Jirong Luo | 2006 | Surface and Coatings Technology2006，200，1819： | 1 |
| 15 | A chrome-free conversion coating of magnesium alloy by a phosphate-permanganate solution显示文摘 | ZHAO Ming WU Shusen LUO Jirong | 2006 | Surface and Coatings Technology2006，200，： | 1 |
| 16 | Microstructure and corrosion resistance of a chromium-free multi-elements complex coating on AZ91D magnesium alloy 显示文摘 | Ming Zhao Shusen Wu Ping An | 2006 | Materials Chemistry and Physics2006，99，1： | 1 |
| 17 | Microstructural and mechanical characteristics of laser welding of Ti6Al4V and lead metal显示文摘 | Shusen Zhao Gang Yu Xiuli He Yaowu Hu | 2012 | Journal of Materials Processing Tech2012，，7： | 1 |
| 18 | Formation of non-dendritic microstructure of semi-solidaluminum alloy under vibration显示文摘 | Wu Shusen Xie Lizhi Zhao Junwen | 2008 | Scripta Materialia2008，58，： | 1 |
| 19 | The Affection of Thickness Error on THz Refractive Index显示文摘 | ZHAO Shusen LIU Xipeng SHEN Jingling | 2009 | Journal of Capital Normal University:Natural Science Edition2009，30，2： | 1 |
| 20 | Microstraucture and Corrosion Resistance of a Chromium-free Multi-elements Complex Coating on AZ91 D Magnesium Alloy显示文摘 | Zhao Ming Wu Shusen | 2006 | Materials Chemistry and Physics2006，99，： | 1 |
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