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| 1 | Reappraisal of phylogenetic status and genetic diversity analysis of Asian population of Lentinula edodes显示文摘在 Lentinula 类以内的种系发生的关系被构造基于定序它 60 中国野 L 的碎片。编辑颂诗孤立, 48 的顺序数据从从情报银行下载的另外的区域不同种类孤立。108 Lentinula 孤立类被划分成二个分支和七个组,一个分支和二个组与词法种的分类显然相应。亚洲人孤立在组被划分我和 V,二五组旧战争,细菌原生质资源地位由代表了是很重要的由种系发生的分析出现。充满地理分发的空白的组 V 与一个增加的孤立的数字成为了主流的组之一,当时我有的组异化进二亚群( Ia 和 Ib )与的一个趋势一巨大孤立数量和大多数测试区域的一篇报道,建议那个中国(或亚洲)是 Lentinula 的自然人口的一个重要基因差异中心类。亚洲人的基因分析基于组孤立 Ia, Ib 和组 V 显示沿海陆地的、西北的高地和西南的中国和喜马拉雅山脉区域是的东方的差异最丰富,它是在亚洲 Lentinulapopulation 的差异保护的三优先级。 | XU Xuefeng LI Anzheng CHENG Shuiming LIN Fanxue LIN Fangcan | 2006 | Progress in Natural Science:Materials International2006，16，3： | 10 |
| 2 | Enhanced RuBisCO activity and promoted dicotyledons growth with degradable carbon dots显示文摘The ~ 5 nm degradable carbon dots (CDs) were synthesized directly from carbon rod by a one-step electrochemical method at room temperature.The as-prepared CDs can effectively enhance the ribulose bisphosphate carboxylase oxygenase (RuBisCO) activity,and then promote the dicotyledons growth (soybean,tomato,eggplant and so on) and finally increase their yields.Here,we used Arabidopsis thaliana and Trifolium repens L.as model plants to systematically study the beneficial effects of CDs on plant growth.These include:(i) accelerating seed germination;(ii) enlarging root elongation;(iii) increasing metal ions absorption and delivery;(iv) improving enzymes activity;(v) enhancing the carbohydrate content;(vi) degradation into plant hormone analogues and CO2;and finally (vii) enhancing the grain production by about 20%. | Hao Li Jian Huang Yang Liu Fang Lu Jun Zhong Yong Wang Shuiming Li Yeshayahu Lifshitz Shuit-Tong Lee Zhenhui Kang | 2019 | Nano Research2019，12，7： | 8 |
| 3 | Genetic analysis of segregation distortion of mating-type factors in Lentinula edodes显示文摘Analyses of the mating-type ratio of protoplast monokaryons to spores in 28 strains of Lentinula edodes were performed through statistical methods. The results showed that the segregation distortion phenomenon commonly exists in both types of Lentinula edodes, 17 out of the 28 samples did not display the expected 1∶1 ratio of protoplast monokaryons; and among them 8 fruiting strains distorted 1∶1∶1∶1 segregation of spores. The degree of distortion in all tested wild strains was significantly lower than that of cultured strains. In fruiting test of positive-negative parent, predominant spores were those with the mating type identical to dikaryotic parent, indicating that the predominance of nuclei mainly depends on the mating type of dikaryotic parent. Data from di-mon mating test verified that monokaryons with acceptor nuclear type are present exclusively in a large amount after dedikaryotization, suggesting that the specificities among B factors and some other unknown factors in cytoplasm are probably responsible for the phenomenon of skewed segregation. | CHENG Shuiming LIN Fanxue XU Xuefeng LI Anzheng LIN Fangcan | 2005 | Progress in Natural Science:Materials International2005，15，8： | 8 |
| 4 | Rapid and accurate detection of African swine fever virus by DNA endonuclease-targeted CRISPR trans reporter assay显示文摘The first case of African swine fever(ASF)outbreak in China was reported in a suburban pig farm in Shenyang in 2018.Since then,the rapid spread and extension of ASF has become the most serious threat fo r the swine industry.Therefore,rapid and accurate detection of African swine fever virus(ASFV)is essential to provide effective strategies to control the disease.In this study,we developed a rapid and accurate ASFV-detection method based on the DNA endonuclease-targeted CRISPR trans reporter(DETECTR)assay.By com bining recombinase polymerase am plification w ith CRISPR-Cas12a proteins,the DETECTR assay dem onstrated a m inim um detection lim it of eight copies with no cross reactivity with other swine viruses.Clinical blood samples were detected by DETECTR assay and showed 100%(30/30)agreement w ith real-tim e polymerase chain reaction assay.The rapid and accurate detection of ASFV may facilitate tim ely eradication measures and strict sanitary procedures to control and prevent the spread of ASF. | Zongjie Li Jianchao Wei Di Di Xin Wang Chenxi Li Beibei Li Yafeng Qiu Ke Liu Feng Gu Minglong Tong Shuiming Wang Xiaodong Wu Zhiyong Ma | 2020 | Acta Biochimica et Biophysica Sinica2020，52，12： | 2 |
| 5 | C-X3-C motif chemokine ligand 1/receptor 1 regulates the M1 polarization and chemotaxis of macrophages after hypoxia/reoxygenation injury显示文摘Background:Macrophages play an important role in renal ischemia reperfusion injury,but the functional changes of macrophages under hypoxia/reoxygenation and the related mechanism are unclear and need to be further clarified.Methods:The effects of hypoxia/reoxygenation on functional characteristics of RAW264.7 macrophages were analyzed through the protein expression detection of pro-inflammatory factors TNF-αand CD80,anti-inflammatory factors ARG-1 and CD206.The functional implications of C-X3-C motif chemokine receptor 1(CX3CR1)down-regulation in hypoxic macrophages were explored using small interfering RNA technology.Significance was assessed by the parametrict-test or nonparametric Mann-Whitney test for two group comparisons,and a one-way ANOVA or the Kruskal-Wallis test for multiple group comparisons.Results:Hypoxia/reoxygenation significantly increased the protein expression of M1-related pro-inflammatory factors TNF-α,CD80 and chemokine C-X3-C motif chemokine ligand 1(CX3CL1)/CX3CR1 and inhibited the protein expression of M2-related anti-inflammatory factors ARG-1 and CD206 in a time-dependent manner in RAW264.7 cells.However,the silencing of CX3CR1 in RAW264.7 cells using specific CX3CR1-siRNA,significantly attenuated the increase in protein expression of TNF-α(P<0.05)and CD80(P<0.01)and the inhibition of ARG-1(P<0.01)and CD206(P<0.01)induced by hypoxia/reoxygenation.In addition,we also found that hypoxia/reoxygenation could significantly enhance the migration(2.2-fold,P<0.01)and adhesion capacity(1.5-fold,P<0.01)of RAW264.7 macrophages compared with the control group,and CX3CR1-siRNA had an inhibitory role(40%and 20%reduction,respectively).For elucidating the mechanism,we showed that the phosphorylation levels of ERK(P<0.01)and the p65 subunit of NF-κB(P<0.01)of the RAW264.7 cells in the hypoxic/reoxygenation group were significantly increased,which could be attenuated by down-regulation of CX3CR1 expression(P<0.01,both).ERK inhibitors also significantly blocked the effects of hypoxic/reoxygenation on the protein expression of M1-related pro-inflammatory factors TNF-α,CD80 and M2-related anti-inflammatory factors ARG-1 and CD206.Moreover,we found that conditioned medium from polarized M1 macrophages induced by hypoxia/reoxygenation,notably increased the degree of apoptosis of hypoxia/reoxygenation-induced TCMK-1 cells,and promoted the protein expression of pro-apoptotic proteins bax(P<0.01)and cleaved-caspase 3(P<0.01)and inhibited the expression of anti-apoptotic protein bcl-2(P<0.01),but silencing CX3CR1 in macrophages had a protective role.Finally,we also found that the secretion of soluble CX3CL1 in RAW264.7 macrophages under hypoxia/reoxygenation was significantly increased.Conclusions:The findings suggest that hypoxia/reoxygenation could promote M1 polarization,cell migration,and adhesion of macrophages,and that polarized macrophages induce further apoptosis of hypoxic renal tubular epithelial cells by regulating of CX3CL1/CX3CR1 signaling pathway. | Shuiming Guo Lei Dong Junhua Li Yuetao Chen Ying Yao Rui Zeng Nelli Shushakova Hermann Haller Gang Xu Song Rong | 2021 | Chronic Diseases and Translational Medicine2021，7，4： | 1 |
| 6 | Influence of flavone extract from cultivated saussurea on learning and memory in a mouse model of Alzheimer's disease A comparison with estradiol benzoate显示文摘The present study established a mouse model of Alzheimer's disease,and investigated the effects of treatment with flavone extract from artificially cultivated saussurea.A positive control group was treated with estradiol benzoate,and learning and memory ability were examined in the 8-arm radial maze.The learning and recognition ability of mice with Alzheimer's disease treated with flavone extract was significantly improved and the number of hippocampal neurons was significantly increased in the flavone-treated and positive control groups compared with the model group.The results indicate that flavone extract from artificially cultivated saussurea can improve learning and memory deficits in mice with Alzheimer's disease,exerting effects similar to those of estradiol benzoate. | Weiqiang Chen Shuiming Gong Yan Li Ming Li Zemin Yang Lirong Zhanf | 2011 | Neural Regeneration Research2011，6，30： | 1 |
| 7 | Baseline correction for digital strong-motion records by using the pre-event portion显示文摘Baseline offset in digital strong-motion acceleration record and initial velocity can produce unrealistic results for ground velocity and displacement derived from the acceleration by integration.A new method is proposed for the baseline correction and initial velocity calculation.It is based on linear least-squares fitting of the pre-event portion of velocity derived from the uncorrected acceleration data.Compared with the conventional method,which is based on removing the mean values of the pre-event portions of the acceleration and velocity traces,this method has clearer physical meaning and better stability. | Li Heng Yao Yunsheng Zheng Shuiming Cai Yongjian Lei Dongning | 2011 | Geodesy and Geodynamics2011，2，1： | 1 |
| 8 | Organ function support in patients with coronavirus disease 2019:Tongji experience显示文摘Coronavirus disease 2019(COVID-19)is a highly contagious disease and a serious threat to human health.COVID-19 can cause multiple organ dysfunction,such as respiratory and circulatory failure,liver and kidney injury,disseminated intravascular coagulation,and thromboembolism,and even death.The World Health Organization reports that the mortality rate of severe-type COVID-19 is over 50%.Currently,the number of severe cases worldwide has increased rapidly,but the experience in the treatment of infected patients is still limited.Given the lack of specific antiviral drugs,multi-organ function support treatment is important for patients with COVID-19.To improve the cure rate and reduce the mortality of patients with severe-and critical-type COVID-19,this paper summarizes the experience of organ function support in patients with severe-and criticaltype COVID-19 in Optical Valley Branch of Tongji Hospital,Wuhan,China.This paper systematically summarizes the procedures of functional support therapies for multiple organs and systems,including respiratory,circulatory,renal,hepatic,and hematological systems,among patients with severe-and critical-type COVID-19.This paper provides a clinical reference and a new strategy for the optimal treatment of COVID-19 worldwide. | Yong Li Fan He Ning Zhou Jia Wei Zeyang Ding Luyun Wang Peng Chen Shuiming Guo Binhao Zhang Xiaoning Wan Wei Zhu 无 Xiaoping Chen Xiaomei Guo Rui Li Shengqing Li Daowen Wang Hui Wang Gang Xu Zhenyu Yin Wenkui Yu Bixiang Zhang Jianping Zhao Jianfeng Zhou | 2020 | Frontiers of Medicine2020，14，2： | 1 |
| 9 | Proteomic and peptidomic analysis of the venom from Chinese tarantula Chilobrachys jingzhao显示文摘 | Zhi Liao Jia Cao Shuiming Li Xiaojun Yan Weijun Hu Quanyuan He Jinjun Chen Jianzhou Tang Jinyun Xie and Songping Liang | 2007 | Proteomics2007，，7： | 1 |
| 10 | A highly practical and reliable nickel catalyst for Suzuki-Miyaura coupling of aryl halides显示文摘 | Zhao Yulong Li You Li Shuiming | 2011 | Advanced Synthesis&Catalysis2011，353，9： | 1 |
| 11 | The national multi-center artificial intelligent myopia prevention and control project显示文摘In recent years,the incidence of myopia has increased at an alarming rate among children and adolescents in China.The exploration of an effective prevention and control method for myopia is in urgent need.With the development of information technology in the past decade,artificial intelligence with the Internet of Things technology(AIoT)is characterized by strong computing power,advanced algorithm,continuous monitoring,and accurate prediction of long-term progression.Therefore,big data and artificial intelligence technology have the potential to be applied to data mining of myopia etiology and prediction of myopia occurrence and development.More recently,there has been a growing recognition that myopia study involving AIoT needs to undergo a rigorous evaluation to demonstrate robust results. | Xun Wang Yahan Yang Yuxuan Wu Wenbin Wei Li Dong Yang Li Xingping Tan Hankun Cao Hong Zhang Xiaodan Ma Qin Jiang Yunfan Zhou Weihua Yang Chaoyu Li Yu Gu Lin Ding Yanli Qin Qi Chen Lili Li Mingyue Lian Jin Ma Dongmei Cui Yuanzhou Huang Wenyan Liu Xiao Yang Shuiming Yu Jingjing Chen Dongni Wang Zhenzhe Lin Pisong Yan Haotian Lin Chinese Association of Artificial Intelligence,Medical Artificial Intelligence Branch of Guangdong Medical Association | 2021 | Intelligent Medicine2021，1，2： | 1 |
| 12 | A Proximity-Triggered Strategy toward Transferable Proteolysis Targeting Chimeras显示文摘Over the past 20 years,great efforts have been invested in developing site-specific approaches to protein modification to dissect protein functions directly and accurately.Here,we report a proximitytriggered group transfer strategy from a sulfonium warhead to a Cysteine(Cys)residue of the target protein.With a guiding ligand,cargoes could be transferred selectively from a sulfonium center onto the Cys residue in the vicinity of their binding interface.The successful thalidomide transfer of sulfonium 1-X could be applied intracellularly for epidermal growth factor receptor degradation,highlighting the potential of group transfer strategy as a suite of chemical biology studies,including cell imaging,protein profiling,and protein degradation by simply employing different transferrable groups. | Yuena Wang Rongtong Zhao Chuan Wan Wei Kang Rui Wang Chengyao Chiang Xiaochun Guo Qi Chang Zhanfeng Hou Yuxin Ye Qinhong Luo Ziyuan Zhou Jianbo Liu Shuiming Li Dongyuan Wang Feng Yin Zigang Li | 2023 | CCS Chemistry2023，5，6： | 0 |
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