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| 1 | Orientation of the peptide formation of N-phosphoryl amino acids in solution显示文摘The peptide formation of N-phosphoryl aminoacids with amino acids proceeds in aqueous solution withoutany coupling reagents. After being separated in sephadex gelcolumn, the phosphoryl dipeptides were analyzed by theelectrospray ionization tandem mass spectrometry (ESIMS/MS). The result demonstrates that phosphoryl dipeptideswere datected in all the reaction systems. It is found tkat theformation of N-phosphoryl dipeptides is oriented: theN-terminal amino acid residues of the N-phosphoryl dipep-tides are from N-phosphoryl amino acids, and the peptideelongation happened at the C-terminal. Only adipeptide, noβ-dipeptide, is formed in the N-phosphoryl dipeptides,showing that α-carboxylic group is activated selectively byN-pbosphorylation. Theoretical calculation shows that thepeptide formation of N-phosphoryl amino acids might hap-pen through a pentu-coordinate carboxylic-phosphoric in-termediate in solution. These results might give some clues tothe stlidy on the origin of proteins and protein | CHEN Zhongzhou TONG Yufeng CHEN Shuibing LI Yanmei CHEN YI ZHAO Yufen WANG Jinfeng | 2002 | Chinese Science Bulletin2002，47，22： | 2 |
| 2 | Self-renewal of embryonic stem cells by a small molecule显示文摘 | Chen Shuibing Do J T Zhang Qisheng | 2006 | Proceed- ings of the National Academy of Sciences of the United States of America2006，103，17： | 1 |
| 3 | Re- versine increases the plasticity of lineage-committed mammalian cells显示文摘 | Chen Shuibing Takanashi S Zhang Qisheng | 2007 | Proceedings of the National Acad- emy of Sciences of the United States of America2007，104，10： | 1 |
| 4 | Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells显示文摘 | Xiaoping Chen Wangxiang Wang Shuibing Li | 2010 | Carbohydrate Polymers2010，80，： | 1 |
| 5 | Free radical scavenging of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes and immunity activities in cervical carcinoma rats显示文摘 | CHEN Xiaoping CHEN Yah LI Shuibing | 2009 | Carbohydrate Polymers2009，77，2： | 1 |
| 6 | Generation of pluripotent stem cells from patients with type 1 diabetes显示文摘 | Rene Maehr Shuibing Chen Melinda Snitow | 2009 | PNAS2009，106，15： | 1 |
| 7 | Frcc radical scavenging of Ganoderma luc/dum polysaccharidcs and its effect on antioxidant cnzymes and immunity activities in cervical carcinoma rots显示文摘 | CHEN Xiaoping CHEN Yan LI Shuibing | 2009 | Carbohydrate Polymers2009，77，2： | 1 |
| 8 | Free radical scavenging of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes and immunity activities in cervical carcinoma rats显示文摘 | CHEN Xiaoping CHEN Yan LI Shuibing | 2009 | Carbohydrate Polymers2009，77，2： | 1 |
| 9 | Optimiza- tion of ultrasound- assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells 显示文摘 | Chen Xiaoping Wang Wanxiang Li Shuibing | 2010 | Carbohydrate Polymers2010，80，3： | 1 |
| 10 | A targetable pathway to eliminate TRA-1-60^(+)/TRA-1-81^(+)chemoresistant cancer cells显示文摘Chemoresistance is a primary cause of treatment failure in pancreatic cancer.Identifying cell surface markers specifically expressed in chemoresistant cancer cells(CCCs)could facilitate targeted therapies to overcome chemoresistance.We performed an antibody-based screen and found that TRA-1-60 and TRA-1-81,two‘stemness’cell surface markers,are highly enriched in CCCs.Further-more,TRA-1-60^(+)/TRA-1-81^(+)cells are chemoresistant compared to TRA-1-60^(-)/TRA-1-81^(-)cells.Transcriptome profiling identified UGT1A10,shown to be both necessary and sufficient to maintain TRA-1-60/TRA-1-81 expression and chemoresistance.From a high-content chemical screen,we identified Cymarin,which downregulates UGT1A10,eliminates TRA-1-60/TRA-1-81 expression,and increases chemosensitivity both in vitro and in vivo.Finally,TRA-1-60/TRA-1-81 expression is highly specific in primary cancer tissue and positively correlated with chemoresistance and short survival,which highlights their potentiality for targeted therapy.Therefore,we discovered a novel CCC surface marker regulated by a pathway that promotes chemoresistance,as well as a leading drug candidate to target this pathway. | Lei Tan Xiaohua Duan Pratyusha Mutyala Ting Zhou Sadaf Amin Tuo Zhang Brian Herbst Gokce Askan Tomer Itkin Zhaoying Xiang Fabrizio Michelassi Michael D.Lieberman Christine AIacobuzio-Donahue Steven DLeach Todd Evans Shuibing Chen | 2023 | Journal of Molecular Cell Biology2023，15，6： | 0 |
| 11 | Artificial Esterase Based on Self-assembly Gel Microspheres Constructed from Chitosan and Amino Acids显示文摘A novel artificial esterase based on chitosan and amino acids was synthesized in the present study. The Fmoc-His and Glu were linked to chitosan by active ester method(AEM). The hydroxide radical in chitosan, imidazole group of Fmoc-His and carboxyl from Glu formed a catalytic center of natural esterase. Gel microspheres were coated with a protective layer and a supporting layer by seiassembly construction function in carboxymethylcellulose sodium(CMCS) solution. As for catalytic activity, chitosan-His-Glu was found to be more efficient than chitosan- His and chitosan-Glu in mimicking the core catalytic sites of natural esterase, and the best ratio(mass ratio) of chitosan-His-Glu:CMCS was 1:3. Furthermore, metal ions, such as Ca^2+, Mg^2+, Fe^2+, etc., were able to improve the catalytic efficiency of artificial esterase. And tlie Lineweaver-Burk plot indicated that the catalytic kinetics of artificial esterase conformed to Michaelis-Menten equation. | CAO Jing WANG Miao CHEN Weihua SHE Yongxin WANG Jing WANG Fengzhong LAO Shuibing | 2019 | Chemical Research in Chinese Universities2019，35，3： | 0 |
| 12 | Modeling endodermal organ development and diseases using human pluripotent stem cell-derived organoids显示文摘Recent advances in development of protocols for directed differentiation from human pluripotent stem cells(hPSCs)to defined lineages,in combination with 3D organoid technology,have facilitated the generation of various endoderm-derived organoids for in vitro modeling of human gastrointestinal development and associated diseases.In this review,we discuss current state-ofthe-art strategies for generating hPSC-derived endodermal organoids including stomach,liver,pancreatic,small intestine,and colonic organoids.We also review the advantages of using this system to model various human diseases and evaluate the shortcomings of this technology.Finally,we emphasize how other technologies,such as genome editing and bioengineering,can be incorporated into the 3D hPSC-organoid models to generate even more robust and powerful platforms for understanding human organ development and disease modeling. | Fong Cheng Pan Todd Evans Shuibing Chen | 2020 | Journal of Molecular Cell Biology2020，12，8： | 0 |
| 13 | Organoid-based chemical approach to dissect the mechanism controlling cellular dynamics显示文摘Organoids are self-organizing in vitrothree-dimensional(3D)tissue culturescontaining multiple types of cells.Com-pared to traditional two-dimensional(2D)cell culture systems,3D organoids bet-ter replicate the architecture,complex-ity,and physiology of an organ(Figure1).ln addition,3D organoids are eas-ier to scale up and more cost-efficientwhen compared to animal models. | Lauretta A.Lacko Shuibing Chen | 2020 | Journal of Molecular Cell Biology2020，12，8： | 0 |
| 14 | Comments on ‘An airway organoid-based screen identifies a role for the HIF1α‒glycolysis axis in SARS-CoV-2 infection’显示文摘Coronavirus disease 2019(COVID-19)has been an ongoing public health crisis since the end of 2019;besides vaccine development,there have been major research efforts focused on developing antiviral therapeutics.Remdesivir was the first US Food and Drug Administration(FDA)-approved antiviral drug for COVID-19.Subsequently,the FDA granted emergency use authorization(EUA)for three monoclonal antibody treatments,including sotrovimab or a combination of casirivimab and imdevimab,or bamlanivimab and etesevimab,each of which targets the coronavirus spike protein to block viral entry.Most recently,Britain granted conditional authorization for the ribonucleoside analog molnupiravir,developed by Merck as a viral replication inhibitor.The protease inhibitor PF-07321332 developed by Pfizer and boosted by ritonavir showed promising results in a phase III clinical trial,reducing the risk of hospitalization or death by 89%compared with placebo. | Xiaohua Duan Hui Wang David D.Ho Robert E.Schwartz Todd Evans Shuibing Chen | 2022 | Journal of Molecular Cell Biology2022，14，1： | 0 |
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