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| 1 | Depositional architecture and developing settings of large-scale incised valley and sub-marine gravity flow systems in the Yinggehai and Qiong-dongnan basins,South China Sea显示文摘In the Ying-Qiong basins in the South China Sea developed a set of giant inner shelf slope and submarine gravity flow deposits in the Ying-Huang formations since the Late Miocene. These deposits can be classified into 9 sequences in which slope slump-debris flow, slope fan and particularly, the large-scale axial basin-floor incised valley fills have been recognized. They were distributed in a distinctive pattern attributed to tectonic control. The development ofshelf slopes and abyssal plains in the basins was related to the last episode of rapid subsidence and a large amount of sediment inputs. Large-scale basin-floor incised valleys, formed during the late Miocene and Pliocene, indicate several major sea level falls that occurred in the South China Sea. | LIN Changsong LIU Jingyan CAI Shixiang ZHANG Yanmei LU Ming LI Jie | 2001 | Chinese Science Bulletin2001，46，8： | 18 |
| 2 | Switchable dual-wavelength Q-switched fiber laser using multilayer black phosphorus as a saturable absorber显示文摘Black phosphorus(BP), with thickness-dependent direct energy bandgaps(0.3–2 eV), shows an enhanced nonlinear optical response at near-and mid-infrared wavelengths. In this paper, we present experimentally multilayer BP flakes coated on microfiber(BCM) as a saturable absorber with a modulation depth of 16% and a saturable intensity of 6.8 MW∕cm^2. After inserting BCM into an Er-doped fiber ring laser, a stable dual-wavelength Q-switched state with central wavelengths of 1542.4 nm and 1543.2 nm(with wavelength spacing as small as 0.8 nm) is obtained with the aid of two cascaded fiber Bragg gratings as a coarse wavelength selector.Moreover, single-wavelength Q-switched operation at 1542.4 nm or 1543.2 nm is also realized, which can be switched between the two wavelengths flexibly just by adjusting the intracavity birefringence. These results suggest that BP combined with the cascaded fiber gratings can provide a simple and feasible candidate for a multiwavelength fiber laser. Our fiber laser may have potential applications in terahertz generation, laser radar,and so on. | JUNMIN LIU Yu CHEN YING LI HAN ZHANG SHUIQIN ZHENG SHIXIANG Xu | 2018 | Photonics Research2018，6，3： | 8 |
| 3 | Differences in the Tectonic Evolution of Basins in the CentralSouthern South China Sea and their Hydrocarbon Accumulation Conditions显示文摘To reveal the causes of differences in the hydrocarbon accumulation in continental marginal basins in the centralsouthern South China Sea,we used gravity-magnetic,seismic,drilling,and outcrop data to investigate the tectonic histories of the basins and explore how these tectonic events controlled the hydrocarbon accumulation conditions in these basins.During the subduction of the Cenozoic proto-South China Sea and the expansion of the new South China Sea,the continental margin basins in the central-southern South China Sea could be classified as one of three types of epicontinental basins:southern extensional-foreland basins,western extensional-strike slip basins,and central extensional-drift basins.Because these basins have different tectonic and sedimentary histories,they also differ in their accumulated hydrocarbon resources.During the Cenozoic,the basin groups in the southern South China Sea generally progressed through three stages:faulting and subsidence from the late Eocene to the early Miocene,inversion and uplift in the middle Miocene,and subsidence since the late Miocene.Hydrocarbon source rocks with marine-continental transitional facies dominated byⅡ-Ⅲkerogen largely developed in extremely thick Miocene sedimentary series with the filling characteristics being mainly deep-water deposits in the early stage and shallow water deposits in the late stage.With well-developed sandstone and carbonate reservoirs,this stratum has a strong hydrocarbon generation potential.During the Cenozoic,the basin groups in the western South China Sea also progressed through the three developmental stages discussed previously.Hydrocarbon source rocks with lacustrine facies,marine-continental transitional facies,and terrigenous marine facies dominated byⅡ2-Ⅲkerogen largely developed in the relatively thick stratum with the filling characteristics being mainly lacustrine deposits in the early stage and marine deposits in the late stage.As a reservoir comprised of self-generated and self-stored sandstone,this unit also has a high hydrocarbon generation potential.Throughout those same three developmental stages,the basin groups in the central South China Sea generated hydrocarbon source rocks with terrigenous marine facies dominated byⅢkerogen that have developed in a stratum with medium thicknesses with the filling characteristics being mainly sandstone in the early stage and carbonate in the late stage.This reservoir,which is dominated by lower-generation and upper-storage carbonate rocks,also has a high hydrocarbon generation potential. | TANG Wu ZHAO Zhigang SONG Shuang WANG Yibo XIE Xiaojun LIU Shixiang | 2021 | Acta Geologica Sinica(English Edition)2021，95，1： | 3 |
| 4 | Hydrocarbon accumulation in the deep waters of South China Sea controlled by the tectonic cycles of marginal sea basins显示文摘The tectonic cycle of the marginal sea basins in South China Sea(SCS)includes two cycles,i.e.,the formation and contraction of Palaeo-SCS and Neo-SCS.The northern part of SCS is a rift basin on a passive continental margin,while the the Nansha Block is a drifting rift basin.The southern part is a compound compressional basin on an active continental margin;the western part is a shear-extensional basin on a transform continental margin;the eastern part is an accretionary wedge basin on a subduction continental margin.The deep-water basins are mainly distributed on the continental slope and the the Nansha Block.There are three sets of source rocks in the deep-water areas of the northern continental margin in SCS,i.e.,Eocene terrestrial facies,early Oligocene transitional facies and late Oligocene marine facies.A set of Late Cretaceous-Early Oligocene terrestrial marine facies source rocks are developed in the drift-rift basin of SCS.Three sets of Oligocene,early Miocene and Mid-Miocene marine-terrestrial transitional facies source rocks are developed in the deep-water areas of both the southern and western continental margins of SCS.Four sets of reservoirs developed in the northern deep waters of SCS are dominated by deep sea fans.Two sets of reservoirs developed in the the Nansha Block are dominated by delta and biogenic reef.The southern part of SCS is dominated by deep sea fan and biogenic reef.Reservoirs of large channels and other clastic facies were developed in front of the estuaries,while biogenic reef bank was formed in the uplift zone.The hydrocarbon accumulation assemblages are mainly presented as Oligocene-Pliocene in the deep waters on the northern continental margin of SCS,Eocene-Lower Oligocene in the the Nansha Block,Oligocene-Pliocene and Oligocene-Miocene in the deep waters on the southern and western continental margin of SCS,respectively.The major hydrocarbon reservoir types in the deep waters of SCS are related to structural traps,deep water fans and biogenic reefs.The formation of basin,hydrocarbon and reservoir in the deep waters of SCS are controlled by the tectonic cycles of the marginal sea basins,revealing a great potential for hydrocarbon exploration. | Gongcheng Zhang Hongjun Qu Shixiang Liu Xiaojun Xie Zhao Zhao Huailei Shen | 2016 | Petroleum Research2016，1，1： | 3 |
| 5 | Characteristics and resource prospects of tight oil in Ordos Basin, China显示文摘Efficient large-scale development of ultra-low-permeability reservoirs(0.3-1 mD)has been achieved in the Changqing Oilfield,Ordos Basin of China.According to unique features of petroleum exploration and development in this basin,tight oil herein refers to petroleum that occurs in oil-bearing shales and interbedded tight sandstone reservoirs adjacent to source rocks with ambient air permeability<0.3 mD.Tight oil in tight sandstone and shale have generally not yet experienced large-scale long-distance migration.In the Yanchang Formation,tight oil has mainly accumulated in the semi-deep to deep lacustrine facies,typically in oil-bearing shales and tight sandstones of the 7th member oil-bearing formation and tight sandstones of the 6th member oil-bearing formation in the center of the basin.Tight oil resource in the Ordos Basin is characterized by wide spatial distribution,excellent source rocks,extremely tight sandstone reservoirs,complex pore throat structures,poor physical properties,high oil saturation,good crude-oil properties,and low reservoir pressure.A fundamental feature of the continuous oil and gas accumulation in tight oil reservoirs is the widespread development of nano-scale pore-throat systems.In the Yanchang Formation,most of connected pore throats in tight sandstone reservoirs have diameters greater than critical pore throat diameter,allowing oil and gas migration in the tight reservoirs.According to contact relationship between tight reservoirs and source rocks,three types of tight oil reservoirs are identified in the Yanchang Formation,i.e.,tight massive sandstone reservoir,sand-shale interbed reservoir,and oil-bearing shale reservoir.In the Ordos Basin,tight oil is widely distributed in the 6th and 7th members of the Yanchang Formation,with total resources estimated to be 3×10^(9) t.These include>1×10^(9) t of oil resources in shale in the 7th member of the Yanchang Formation and approximately 0.9×10^(9) t and 1.1×10^(9) t of tight sandstone oil resource in the 6th and 7th members of the Yanchang Formation,respectively.These tight oil resources are the realistic resources addition for the oilfield,which can ensure an annual production of 50×10^(6) t of oil and gas equivalent and maintain long-term stable oil production in the Changqing Oilfield,Ordos Basin,China. | Hua Yang Shixiang Li Xianyang Liu | 2016 | Petroleum Research2016，1，1： | 2 |
| 6 | Intelligent Resources Management System Design in Information Centric Networking显示文摘Information centric networking(ICN) is a new network architecture that is centred on accessing content. It aims to solve some of the problems associated with IP networks, increasing content distribution capability and improving users' experience. To analyse the requests' patterns and fully utilize the universal cached contents, a novel intelligent resources management system is proposed, which enables effi cient cache resource allocation in real time, based on changing user demand patterns. The system is composed of two parts. The fi rst part is a fi ne-grain traffi c estimation algorithm called Temporal Poisson traffi c prediction(TP2) that aims at analysing the traffi c pattern(or aggregated user requests' demands) for different contents. The second part is a collaborative cache placement algorithm that is based on traffic estimated by TP2. The experimental results show that TP2 has better performance than other comparable traffi c prediction algorithms and the proposed intelligent system can increase the utilization of cache resources and improve the network capacity. | Hengyang Zhang Shixiang Zhu Renchao Xie Tao Huang Yunjie Liu | 2017 | China Communications2017，14，8： | 2 |
| 7 | Implementation method of Oil and Gas Geologic Information System with ArcGIS Engine显示文摘ComGIS is the mainstream of developing GIS currently.Developing Geographic Information System with the technology of components can reduce the difficulties of developing,improving the developing efficiency and enhancing the flexibility and opening of system.ArcObjects(AO) is based on the technology of COM,and ArcGIS Engine(AE) is a set of embedded AO which can extend to various platforms.Compared with AO,ArcGIS Engine can shorten the period of developing and improve the efficiency greatly.Having introduced the techniques of ArcObjects and ArcGIS Engine,we offer the method of developing GIS with Visual Basic and ArcGIS Engine with Oil and Gas Geologic Information System. | Chunyan DENG Yunliang YU Shixiang LIU Ruiqing QIE Zhiguo ZHOU | 2007 | Global Geology2007，10，1： | 2 |
| 8 | Progress in the beam commissioning of separated function RFQ accelerator显示文摘Separated Function RFQ (SFRFQ) was proposed as a post accelerator of RFQ to accelerate heavy ions at low frequency. It introduces gap accelerating in the quadrupole electrodes, and therefore it has higher accelerating efficiency than the conventional RFQ accelerator. The first SFRFQ prototype cavity has been specially designed and constructed as a post accelerator to accelerate O+ beam from 1.03 MeV to 1.64 MeV. Based on accomplishment of low power measurement and high power test, the beam commissioning was carried out to verify its feasibility. The measured energy gain per cell of SFRFQ is 45 keV, which is about 60% higher than that of Peking University Integral Split Ring (ISR) RFQ. | MingLei Kang (1) YuanRong Lu (1) Zhi Wang (1) Kun Zhu (1) XueQing Yan (1) ZhiYu Guo (1) ShuLi Gao (1) ShiXiang Peng (1) Ao Liu (2) JiaXun Fang (1) JiaEr Chen (1) | 2011 | Science China(Physics,Mechanics & Astronomy)2011，54，S2： | 2 |
| 9 | Controlled growth of superhydrophobic films by sol–gel method on aluminum substrate显示文摘 | Shixiang Lu Yiling Chen Wenguo Xu Wei Liu | 2010 | Applied Surface Science2010，，20： | 1 |
| 10 | The gradual subduction-collision evolution model of Proto-South China Sea and its control on oil and gas显示文摘This study involved outcrop,drilling,seismic,gravity,and magnetic data to systematically document the geological records of the subduction process of Proto-South China Sea(PSCS)and establish its evolution model.The results indicate that a series of arc-shaped ophiolite belts and calcalkaline magmatic rocks are developed in northern Borneo,both of which have the characteristics of gradually changing younger from west to east,and are direct signs of subduction and collision of PSCS.At the same time,the subduction of PSCS led to the formation of three accretion zones from the south to the north in Borneo,the Kuching belt,Sibu belt,and Miri belt.The sedimentary formation of northern Borneo is characterized by a three-layer structure,with the oceanic basement at the bottom,overlying the deep-sea flysch deposits of the Rajang–Crocker group,and the molasse sedimentary sequence that is dominated by river-delta and shallow marine facies at the top,recording the whole subduction–collision–orogeny process of PSCS.Further,seismic reflection and tomography also confirmed the subduction and collision of PSCS.Based on the geological records of the subduction and collision of PSCS,combined with the comprehensive analysis of segmented expansion and key tectonic events in the South China Sea,we establish the“gradual”subduction-collision evolution model of PSCS.During the late Eocene to middle Miocene,the Zengmu,Nansha,and Liyue–Palawan blocks were separated by West Baram Line and Balabac Fault,which collided with the Borneo block and Kagayan Ridge successively from the west to the east,forming several foreland basin systems,and PSCS subducted and closed from the west to the east.The subduction and extinction of PSCS controlled the oil and gas distribution pattern of southern South China Sea(SSCS)mainly in three aspects.First,the“gradual”closure process of PSCS led to the continuous development of many large deltas in SSCS.Second,the deltas formed during the subduction–collision of PSCS controlled the development of source rocks in the basins of SSCS.Macroscopically,the distribution and scale of deltas controlled the distribution and scale of source rocks,forming two types of source rocks,namely,coal measures and terrestrial marine facies.Microscopically,the difference of terrestrial higher plants carried by the delta controlled the proportion of macerals of source rocks.Third,the difference of source rocks mainly controlled the distribution pattern of oil and gas in SSCS.Meanwhile,the difference in the scale of source rocks mainly controlled the difference in the amount of oil and gas discoveries,resulting in a huge amount of oil and gas discoveries in the basin of SSCS.Meanwhile,the difference of macerals of source rocks mainly controlled the difference of oil and gas generation,forming the oil and gas distribution pattern of“nearshore oil and far-shore gas”. | Xiaojun Xie Wu Tang Gongcheng Zhang Zhigang Zhao Shuang Song Shixiang Liu Yibo Wang Jia Guo | 2023 | Acta Oceanologica Sinica2023，42，3： | 1 |
| 11 | An experimental study on synthesizing submicron MoSi2-based coatings using electrothermal explosion ultra-high speed spraying method显示文摘 | Liu Zongde Hou Shixiang Liu Dongyu | | 0，，13： | 1 |
| 12 | Interpretation of remote sensing of geological structures and prediction of gold mineralization in Mohe area and its adjacent region显示文摘The authors took the ETM+ multi-spectra data as the data information and correlation coefficient for each band and carried out their information volume statistics.According to certain criteria,the authors also determined the optimum band-combined image.The image clarity is improved by various enhancements and fusions method.Based on remote sensing geological interpretation in detail,the relationship between remote sensing geological characters and gold mine were analyzed systemically.Using all kinds of remote sensing structure information,combining other research data,the authors determined mainly ore-controlling ore structure.Several prospective areas of gold ores were determined and furthermore significant finding mine target areas was confirmed. | Ruiqing QIE Linfu XUE Shixiang LIU Yunliang YU | 2007 | Global Geology2007，10，1： | 1 |
| 13 | HD-Zip Transcription Factor is Responsible for No-Lobed Leaf in Watermelon(Citrullus lanatus L.)显示文摘Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in most crops.In this study,two-lobed leaf watermelon inbred lines WT2 and WCZ,and a no-lobed leaf watermelon inbred line WT20 were used to create two F_(2)populations.Segregation analysis suggested that lobed leaves were dominant over the no-lobed leaves,and it was controlled by a signal gene.A locus on watermelon chromosome 4 controlling watermelon lobed/no-lobed leaves was identified through BSA-seq strategy combined with linkage analysis.The candidate gene was fine-mapped to a 61.5 kb region between 21,224,481 and 21,285,957 bp on watermelon chromosome 4 using two F_(2)populations.Four functional genes were annotated in the candidate region,while sequences blast showed that there was a single-base deletion(A/-)only in the exon of Cla018360,which resulted in premature termination of translation in the no-lobed leaf lines.Function prediction showed that Cla018360 encodes an HD-Zip protein that has been reported to regulate the development of leaf shape.The single-base deletion also occurred in the HD-Zip domain.We inferred that the Cla018360 gene is the candidate gene for regulating the development of lobed/no-lobed leaves in watermelon.Gene expression analysis showed that Cla018360 was highly expressed in young leaves.Phylogenetic analysis showed that Cla018360 had a close genetic relationship with AtHB51,which had been reported to regulate the formation of leaf shape in Arabidopsis.Furthermore,transcriptome analysis showed that a total of 333 differentially expressed genes were identified between WT2 and WT20,of which 115 and 218 genes were upregulated and downregulated in no-lobed leaved watermelon WT20.This study not only provides a good entry point for studying leaf development but also provides foundational insights into breeding for special plant architecture in watermelon. | Shixiang Duan Yaomiao Guo Yinping Wang Muhammad Jawad Umer Dongming Liu Sen Yang Huanhuan Niu Shouru Sun Luming Yang Junling Dou Huayu Zhu | 2023 | Phyton-International Journal of Experimental Botany2023，92，5： | 1 |
| 14 | Identification of sweet spots in shale-type and siltstone-type“shale oil systems”:A case study of the Chang 7 Member in Ordos Basin显示文摘Mid-high maturity shale oil is the most realistic field for the scale breakthrough of terrestrial shale oil production in China.Generally,three deficiencies hinder shale oil development in China:heavy oil density,small sweet spot areas,and poor distribution continuity.Thus,identifying the“sweet spots”in shale oil reservoirs is critical for the efficient exploration and development of terrestrial shale oil.This study targets the siltstone type(Class-Ⅱshale oil)and pure shale type(Class-Ⅲshale oil)of the Chang 7 Member in the Ordos Basin,and identifies three stratigraphic units,namely the hydrocarbon accumulation unit,hydrocarbon generation unit,and hydrocarbon retention unit,which together constitute the in-source“shale oil system”.The hydrocarbon accumulation unit is mainly siltstone,where the hydrocarbons are migrated from shales.It has favorable porethroat network connectivity with a pore connectivity ratio of 32–57%,being the siltstone-type sweet spots.The hydrocarbon generation unit is mainly composed of high-TOC mudstone/shale and is the main contributor to in-source hydrocarbon generation and expulsion.This unit has high three-dimensional connectivity(28–30%),as shown by the pore-throat network model,associated with vertical paths for hydrocarbon expulsion.The hydrocarbon retention unit is mainly composed of low-TOC mudstone/shale retaining self-generated and migrated hydrocarbons.The pore connectivity rate is 17–42%,and the pore-throat network connectivity direction is uneven.Light and low-carbon-number hydrocarbons are preferentially trapped or even sealed in small pores of the retention unit,forming the typical mudstone/shale-type sweet spots.In the process called shale oil intrasource migration,the oil migrates in source rocks causing component fractionation,which allows more shale oil to enrich in the hydrocarbon accumulation and retention units to form sweet spots,compared with the hydrocarbon generation unit.The migration paths include the one from mudstone/shale to siltstone interlayers and that from the high-TOC mudstone/shale intervals to the low-TOC intervals.The in-source accumulation of shale oil shows the differentiated enrichment model featuring“high-TOC mudstone/shale generating hydrocarbons,low-TOC mudstone/shale retaining hydrocarbons,siltstone accumulating hydrocarbons and multiple intra-source migration paths”.In the Ordos Basin,the organic-lean(TOC 1–3%)mudstone/shale intervals appear to be the sweet spots of shale oil,where there are abundant medium-short-chain hydrocarbons retained with high flowability.After fracturing stimulation,their production conditions may be even superior to those of siltstones.This proposed idea changes the previous strategy to look for sweet spots in high-TOC intervals derived from the shale gas industry. | Songqi PAN Qiulei GUO Caineng ZOU Zhenhua JING Ming YUAN Ying HE He ZHENG Ying MU Zhi YANG Shixiang LI Xinping ZHOU Songtao WU Hanlin LIU Feng YANG Yuanjia HAN | 2023 | Science China Earth Sciences2023，66，7： | 1 |
| 15 | Synthesis and lu- minescence properties of Eu2+ , Sma+ doped ( YxGd1-x )203 : Si4+,Mg2+ long lasting phosphor显示文摘 | Liu Yan Liu Shixiang Wang Mingwen | 2010 | International Journal o{ Minerals Metallurgy and Materials2010，17，3： | 1 |
| 16 | Luminescent low temperature co-fired ceramics for high power LED package显示文摘 | DING Youyi LIU Shixiang LI Xingyun | 2012 | Journal of Alloys and Compounds2012，521，25： | 1 |
| 17 | World's Energy Transition and Chinese-style Modern Energy Revolution under Carbon Neutrality(Ⅰ)显示文摘Under global consensus on carbon neutrality and the intensification of regional conflicts,new energy has become the primary direction for various countries to achieve energy security and green development.Represented by wind,solar,geothermal,hydrogen and stored energy,and controllable nuclear fusion,the technological and scale advantages of new energy are being continuously strengthened.Low-cost wind,solar,and stored energy will support the global energy transition.The production and utilization of new energy enter a rapid development phase.In 2022,the average global consumption of new energy accounted for 18.2%,and it is expected to reach around 55%by 2050.The distribution of the world's energy resources,consumption regions,technological development,per capita consumption,energy-saving fields,and carbon emissions is uneven.The acceleration of low-carbon development in fossil fuels,the scale-up of renewable energy,and the intelligentization of energy management drives the rapid transition of global energy. | Zou Caineng Xiong Bo Li Shixiang Ma Feng Pan Songqi Liu Hanlin Zhang Guosheng Zhao Qun Guan Chunxiao | 2024 | China Oil & Gas2024，31，1： | 0 |
| 18 | Visualization of supramolecular assembly by aggregation-induced emission显示文摘Supramolecular architectures are constructed by the self-assembly of small building blocks via the use of metal-ligand coordination,π–πstacking interactions,hydrogen bonding,host-guest interactions,and other noncovalent driving forces,which confer unique dynamic reversibility and stimulus responsiveness to the supramolecular materials and also lead to the demand of expensive and complex equipment for the characterization of supramolecular assembly processes.Fortunately,the self-assembly processes bring the monomeric chromophores together,offering possibilities to establish ties between the supramolecular assembly and aggregation-induced emission(AIE)techniques.Compared to conventional luminescent molecules,AIE luminogens(AIEgens)exhibit significant fluorescence enhancement upon the restriction of molecular motions,thus displaying the advantages of signal amplification and low background noises.Given the above,the real-time,sensitive,and in situ visualization of the formation of self-assemblies and their stimuli responsiveness based on AIE becomes accessible.Here,we review recent works that encompass the visualization of supramolecular assembly-related behaviors by means of AIE characteristics of chromophores.The organization of this review will be by different types of supramolecular architectures,including metallacycles/cages,micelles/vesicles,supramolecular polymers,and supramolecular gels.An overview of future opportunities and challenges for the real-time monitoring of supramolecular assembly by AIE is also provided. | Wu-Jie Guo Tuokai Peng Wenping Zhu Shixiang Ma Guang Wang Ying Li Bin Liu Hui-Qing Peng | 2023 | Aggregate2023，4，2： | 0 |
| 19 | Logging data representation based on XML显示文摘As an open standard of data representation,XML breathes new energy to the Web application and the network computing.The development,advantage and status of XML and some standards relating to XML are reviewed.In addition,the authors put forward a method representing logging data and using UML language to establish the conceptual and logical model of logging data;using a logging data,explain how to establish the model as well as how to use XML to display and process geology data. | Shixiang LIU Linfu XUE Ruiqing QIE Jinping MA | 2007 | Global Geology2007，10，1： | 0 |
| 20 | Antagonistic MADS-box transcription factors SEEDSTICK and SEPALLATA3 form a transcriptional regulatory network that regulates seed oil accumulation显示文摘Transcriptional regulation is essential for balancing multiple metabolic pathways that influence oil accumulation in seeds.Thus far,the transcriptional regulatory mechanisms that govern seed oil accumulation remain largely unknown.Here,we identified the transcriptional regulatory network composed of MADS-box transcription factors SEEDSTICK(STK)and SEPALLATA3(SEP3),which bridges several key genes to regulate oil accumulation in seeds.We found that STK,highly expressed in the developing embryo,positively regulates seed oil accumulation in Arabidopsis(Arabidopsis thaliana).Furthermore,we discovered that SEP3 physically interacts with STK in vivo and in vitro.Seed oil content is increased by the SEP3 mutation,while it is decreased by SEP3 overexpression.The chromatin immunoprecipitation,electrophoretic mobility shift assay,and transient dual-luciferase reporter assays showed that STK positively regulates seed oil accumulation by directly repressing the expression of MYB5,SEP3,and SEED FATTY ACID REDUCER 4(SFAR4).Moreover,genetic and molecular analyses demonstrated that STK and SEP3 antagonistically regulate seed oil production and that SEP3 weakens the binding ability of STK to MYB5,SEP3,and SFAR4.Additionally,we demonstrated that TRANSPARENT TESTA 8(TT8)and ACYL-ACYL CARRIER PROTEIN DESATURASE 3(AAD3)are direct targets of MYB5 during seed oil accumulation in Arabidopsis.Together,our findings provide the transcriptional regulatory network antagonistically orchestrated by STK and SEP3,which fine tunes oil accumulation in seeds. | Shuangcheng He Yuanchang Min Zijin Liu Fang Zhi Rong Ma Ankang Ge Shixiang Wang Yu Zhao Danshuai Peng Da Zhang Minshan Jin Bo Song Jianjun Wang Yuan Guo Mingxun Chen | 2024 | Journal of Integrative Plant Biology2024，66，1： | 0 |
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