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您的检索式：作者名="Sebastian A.J.Zaat"
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| 1 | Antibacterial photodynamic therapy:overview of a promising approach to fight antibiotic-resistant bacterial infections显示文摘Antibacterial photodynamic therapy(APDT)has drawn increasing attention from the scientific society for its potential to effectively kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance that bacteria can rapidly develop against traditional antibiotic therapy.The review summarizes the mechanism of action of APDT,the photosensitizers,the barriers to PS localization,the targets,the in vitro-,in vivo-,and clinical evidence,the current developments in terms of treating Gram-positive and Gram-negative bacteria,the limitations,as well as future perspectives.Relevance for patients:A structured overview of all important aspects of APDT is provided in the context of resistant bacterial species.The information presented is relevant and accessible for scientists as well as clinicians,whose joint effort is required to ensure that this technology benefits patients in the post-antibiotic era. | Yao Liu Rong Qin Sebastian A.J.Zaat Eefjan Breukink Michal Heger | 2015 | Journal of Clinical & Translational Research2015，1，3： | 8 |
| 2 | Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels显示文摘We report on the fabrication and characterization of color-encoded chitosan hydrogels for the rapid,sensitive and specific detection of bacterial enzymes as well as the selective detection of a set of tested bacteria through characteristic enzyme reactions.These patterned sensor hydrogels are functionalized with three different colorimetric enzyme substrates affording the multiplexed detection and differentiation ofα-glucosidase,β-galactosidase andβ-glucuronidase.The limits of detection of the hydrogels for an observation time of 60 min using a conventional microplate reader correspond to concentrations of 0.2,3.4 and 4.5 nM of these enzymes,respectively.Based on their different enzyme expression patterns,Staphylococcus aureus strain RN4220,methicillin-resistant S.aureus(MRSA)strain N315,both producingα-glucosidase,but notβ-glucuronidase andβ-galactosidase,Escherichia coli strain DH5α,producingβ-glucuronidase andα-glucosidase,but notβ-galactosidase,and the enterohemorrhagic E.coli(EHEC)strain E32511,producingβ-galactosidase,but none of the other two enzymes,can be reliably and rapidly distinguished from each other.These results confirm the applicability of enzyme sensing hydrogels for the detection and discrimination of specific enzymes to facilitate differentiation of bacterial strains.Patterned hydrogels thus possess the potential to be further refined as detection units of a multiplexed format to identify certain bacteria for future application in point-of-care microbiological diagnostics in food safety and medical settings. | Zhiyuan Jia Mareike Müller Tony Le Gall Martijn Riool Max Müller Sebastian A.J.Zaat Tristan Montier Holger Schönherr | 2021 | Bioactive Materials2021，6，12： | 0 |
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