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| 1 | Three-dimensional bioprinting collagen/silk fibroin scaffold combined with neural stem cells promotes nerve regeneration after spinal cord injury显示文摘Many studies have shown that bio-scaffolds have important value for promoting axonal regeneration of injured spinal cord.Indeed,cell transplantation and bio-scaffold implantation are considered to be effective methods for neural regeneration.This study was designed to fabricate a type of three-dimensional collagen/silk fibroin scaffold (3D-CF) with cavities that simulate the anatomy of normal spinal cord.This scaffold allows cell growth in vitro and in vivo.To observe the effects of combined transplantation of neural stem cells (NSCs) and 3D-CF on the repair of spinal cord injury.Forty Sprague-Dawley rats were divided into four groups: sham (only laminectomy was performed),spinal cord injury (transection injury of T10 spinal cord without any transplantation),3D-CF (3D scaffold was transplanted into the local injured cavity),and 3D-CF + NSCs (3D scaffold co-cultured with NSCs was transplanted into the local injured cavity.Neuroelectrophysiology,imaging,hematoxylin-eosin staining,argentaffin staining,immunofluorescence staining,and western blot assay were performed.Apart from the sham group,neurological scores were significantly higher in the 3D-CF + NSCs group compared with other groups.Moreover,latency of the 3D-CF + NSCs group was significantly reduced,while the amplitude was significantly increased in motor evoked potential tests.The results of magnetic resonance imaging and diffusion tensor imaging showed that both spinal cord continuity and the filling of injury cavity were the best in the 3D-CF + NSCs group.Moreover,regenerative axons were abundant and glial scarring was reduced in the 3D-CF + NSCs group compared with other groups.These results confirm that implantation of 3D-CF combined with NSCs can promote the repair of injured spinal cord.This study was approved by the Institutional Animal Care and Use Committee of People’s Armed Police Force Medical Center in 2017 (approval No.2017-0007.2). | Ji-Peng Jiang Xiao-Yin Liu Fei Zhao Xiang Zhu Xiao-Yin Li Xue-Gang Niu Zi-Tong Yao Chen Dai Hui-You Xu Ke Ma Xu-Yi Chen Sai Zhang | 2020 | Neural Regeneration Research2020，15，5： | 14 |
| 2 | Collagen scaffold combined with human umbilical cord-mesenchymal stem cells transplantation for acute complete spinal cord injury显示文摘Currently, there is no effective strategy to promote functional recovery after a spinal cord injury. Collagen scaffolds can not only provide support and guidance for axonal regeneration, but can also serve as a bridge for nerve regeneration at the injury site. They can additionally be used as carriers to retain mesenchymal stem cells at the injury site to enhance their effectiveness. Hence, we hypothesized that transplanting human umbilical cord-mesenchymal stem cells on collagen scaffolds would enhance healing following acute complete spinal cord injury. Here, we test this hypothesis through animal studies and a phase I clinical trial.(1) Animal experiments: Models of completely transected spinal cord injury were established in rats and canines by microsurgery. Mesenchymal stem cells derived from neonatal umbilical cord tissue were adsorbed onto collagen scaffolds and surgically implanted at the injury site in rats and canines;the animals were observed after 1 week–6 months. The transplantation resulted in increased motor scores, enhanced amplitude and shortened latency of the motor evoked potential, and reduced injury area as measured by magnetic resonance imaging.(2) Phase I clinical trial: Forty patients with acute complete cervical injuries were enrolled at the Characteristic Medical Center of Chinese People's Armed Police Force and divided into two groups. The treatment group(n = 20) received collagen scaffolds loaded with mesenchymal stem cells derived from neonatal umbilical cordtissues;the control group(n = 20) did not receive the stem-cell loaded collagen implant. All patients were followed for 12 months. In the treatment group, the American Spinal Injury Association scores and activities of daily life scores were increased, bowel and urinary functions were recovered, and residual urine volume was reduced compared with the pre-treatment baseline. Furthermore, magnetic resonance imaging showed that new nerve fiber connections were formed, and diffusion tensor imaging showed that electrophysiological activity was recovered after the treatment. No serious complication was observed during follow-up. In contrast, the neurological functions of the patients in the control group were not improved over the follow-up period. The above data preliminarily demonstrate that the transplantation of human umbilical cord-mesenchymal stem cells on a collagen scaffold can promote the recovery of neurological function after acute spinal cord injury. In the future, these results need to be confirmed in a multicenter, randomized controlled clinical trial with a larger sample size. The clinical trial was approved by the Ethics Committee of the Characteristic Medical Center of Chinese People's Armed Police Force on February 3, 2016(approval No. PJHEC-2016-A8). All animal experiments were approved by the Ethics Committee of the Characteristic Medical Center of Chinese People's Armed Police Force on May 20, 2015(approval No. PJHEC-2015-D5). | Wu-Sheng Deng Ke Ma Bing Liang Xiao-Yin Liu Hui-You Xu Jian Zhang Heng-Yuan Shi Hong-Tao Sun Xu-Yi Chen Sai Zhang | 2020 | Neural Regeneration Research2020，15，9： | 11 |
| 3 | A new type of ERGIC–ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis显示文摘Under stress,the endomembrane system undergoes reorganization to support autophagosome biogenesis,which is a central step in autophagy.How the endomembrane system remodels has been poorly understood.Here we identify a new type of membrane contact formed between the ER-Golgi intermediate compartment(ERGIC)and the ER-exit site(ERES)in the ER-Golgi system,which is essential for promoting autophagosome biogenesis induced by different stress stimuli.The ERGIC-ERES contact is established by the interaction between TMED9 and SEC12 which generates a short distance opposition(as close as 2-5 nm)between the two compartments.The tight membrane contact allows the ERES-located SEC12 to transactivate COPII assembly on the ERGIC.In addition,a portion of SEC12 also relocates to the ERGIC.Through both mechanisms,the ERGIC-ERES contact promotes formation of the ERGIC-derived COPII vesicle,a membrane precursor of the autophagosome.The ERGIC-ERES contact is physically and functionally different from the TFG-mediated ERGIC-ERES adjunction involved in secretory protein transport,and therefore defines a unique endomembrane structure generated upon stress conditions for autophagic membrane formation. | Shulin Li Rui Yan Jialu Xu Shiqun Zhao Xinyu Ma Qiming Sun Min Zhang Ying Li Jun-Jie Gogo Liu Liangyi Chen Sai Li Ke Xu Liang Ge | 2022 | Cell Research2022，32，2： | 3 |
| 4 | Effects of cannabinoid receptor type 2 on endogenous myocardial regeneration by activating cardiac progenitor cells in mouse infarcted heart显示文摘Cannabinoid receptor type 2(CB2)activation is recently reported to promote proliferation of some types of resident stem cells(e.g.,hematopoietic stem/progenitor cell or neural progenitor cell).Resident cardiac progenitor cell(CPC)activation and proliferation are crucial for endogenous cardiac regeneration and cardiac repair after myocardial infarction(MI).This study aims to explore the role and possible mechanisms of CB2receptor activation in enhancing myocardial repair.Our results revealed that CB2receptor agonist AM1241 can significantly increase CPCs by c-kit and Runx1 staining in ischemic myocardium as well as improve cardiomyocyte proliferation.AM1241 also decreased serum levels of MDA,TNF-αand IL-6 after MI.In addition,AM1241 can ameliorate left ventricular ejection fraction and fractional shortening,and reduce fibrosis.Moreover,AM1241 treatment markedly increased p-Akt and HO-1 expression,and promoted Nrf-2 nuclear translocation.However,PI3K inhibitor wortmannin eliminated these cardioprotective roles of AM1241.In conclusion,AM1241 could induce myocardial regeneration and improve cardiac function,which might be associated with PI3K/Akt/Nrf2 signaling pathway activation.Our findings may provide a promising strategy for cardiac endogenous regeneration after MI. | WANG YaBin MA Sai WANG Qiang HU WenXing WANG DongJuan LI XiuJuan SU Tao QIN Xing ZHANG XiaoTian MA Ke CHEN JiangWei XIONG LiZe CAO Feng | 2014 | Science China(Life Sciences)2014，57，2： | 3 |
| 5 | Oxygen vacancy engineering of calcium cobaltate:A nitrogen fixation electrocatalyst at ambient condition in neutral electrolyte显示文摘In order to sustainably transform N2 to ammonia(NRR)using electrocatalysts under mild ambient condition,it is urgent to design and develop non-nobel metal nanocatalysts that are inexpensive and suitable for mass-production.Herein,a calcium metalate catalyst CaCoO_(x)with oxygen vacancies was synthesized and used as an electrocatalyst for NRR for the first time,whose morphology can be controlled by the calcination temperature and the heating rate.Under the optimal conditions,the CaCoO_(x)catalyst achieved the yield of nitrogen conversion to ammonia of 16.25μg·h^(-1)·mg_(cat.)^(-1)at the potential of-0.3 V relative to the reversible hydrogen electrode(RHE)with a Faraday efficiency of 20.51%.The electrocatalyst showed good stability even after 12 times recyclability under environmental conditions and neutral electrolyte.Later,the electrocatalytic nitrogen reduction performance of CaFeO_(x),CaNiO_(x),CaCuO_(x)was investigated.These earth-rich transition metals also exhibited certain NRR electrocatalytic capabilities,which provided a door for further development of inexpensive and easily available transition metal as nitrogen reduction electrocatalysts. | Xinyu Chen Ke Li Xiaoxuan Yang Jiaqi Lv Sai Sun Siqi Li Dongming Cheng Bo Li Yang-Guang Li Hong-Ying Zang | 2021 | Nano Research2021，14，2： | 2 |
| 6 | Heterotopie autologous chondrocyte transplantation-a realistic approach to support articular cartilage repair? 显示文摘 | Sayed KE Haiseh A John T | 2010 | Tissue Engineering-Part B: Reviews2010，16，6： | 1 |
| 7 | miR-181b modulates glioma cell sensitivity to temozolomide by targeting MEK1显示文摘 | Jie Wang Ke Sai Fu-rong Chen Zhong-ping Chen | 2013 | Cancer Chemotherapy and Pharmacology2013，，： | 1 |
| 8 | Long-term molecular changes in WHO grade II astrocytomas following radiotherapy显示文摘Monitoring the long-term radiotherapy-associated molecular changes in low-grade gliomas(LGGs) facilitates the understanding of LGG response to radiotherapy.In this study,we used immunohistochemistry to analyze the expression of Ki-67,tumor protein P53(TP53),P21,and P27 in 8 paired WHO grade II astrocytoma samples.The interval between radiotherapy(RT) and the second surgery was more than 3 months in all cases.The average Ki-67 labeling index(LI) was 5.3% in pre-RT samples and 11.54% in post-RT samples.Ki-67 LI was higher in the primary tumors that underwent malignant transformation observed at the second surgery after radiation.Post-RT Ki-67 LI decreased in 2 cases with an interval of less than 12 months between RT and the second surgery.TP53 expression was found in 3 out of 4 pre-RT samples with malignant transformation and in 1 out of 4 pre-RT samples without malignant transformation.Post-RT TP53 increased in 2 cases in which increased expression of P21 or P27 was also observed.Our study suggests that radiotherapy can inhibit WHO grade II astrocytoma proliferation as reflected by Ki-67 LI,but the effect attenuates with time.In addition,there is a tendency of malignant transformation for WHO grade II astrocytomas with a high Ki-67 level or TP53 expression in initial samples. | Wei-Ying Yue Ke Sai Qiu-Liang Wu Yun-Fei Xia Su-Huan Yu Zhong-Ping Chen | 2012 | Chinese Journal of Cancer2012，31，3： | 1 |
| 9 | Marine natural products as prototype agrochemical agents显示文摘 | Peng JG Shen XY Sayed KE | 2003 | J Agric Food Chem2003，51，8： | 1 |
| 10 | Diagnosis, Prognosis and Therapeutic Role of Circulating miRNAs in Cardiovascular Diseases显示文摘 | Ali Sheikh Md Sayed Ke Xia Umme salma Tianlun Yang Jun Peng | 2014 | Heart, Lung and Circulation2014，，： | 1 |
| 11 | Light and elec-tron microscopic study of primary atrophic rhinitis mucosa显示文摘 | Sayed RH Abou-Elhamd KE Makhlouf MM | 2006 | Am J Rhinol2006，20，5： | 1 |
| 12 | Light and electron microscopic study of primary atrophic rhinitis mucosa 显示文摘 | SAYED RH ABOU-ELHAMD KE MAKHLOUF MM | 2006 | Am J Rhinol2006，20，5： | 1 |
| 13 | Retinol binding protein 4 promotes hyperinsulinism induced proliferation of rat aortic smooth muscle cells 显示文摘 | Fei Li Ke Xia Md Sayed Ali Sheikh | 2014 | Mol Med Rep2014，9，5： | 1 |
| 14 | Neurological functional evaluation based on accurate motions in big animals with traumatic brain injury显示文摘An accurate and effective neurological evaluation is indispensable in the treatment and rehabilitation of traumatic brain injury. However,most of the existing evaluation methods in basic research and clinical practice are not objective or intuitive for assessing the neurological function of big animals, and are also difficult to use to qualify the extent of damage and recovery. In the present study, we established a big animal model of traumatic brain injury by impacting the cortical motor region of beagles. At 2 weeks after successful modeling, we detected neurological deficiencies in the animal model using a series of techniques, including three-dimensional motion capture, electromyogram and ground reaction force. These novel technologies may play an increasingly important role in the field of traumatic brain injury diagnosis and rehabilitation in the future. The experimental protocol was approved by the Animal Care and Use Committee of Logistics University of People's Armed Police Force(approval No. 2017-0006.2). | Ji-Peng Jiang Xue-Gang Niu Chen Dai Ke Ma Hui-You Xu Shi-Xiang Cheng Zhi-Wen Zhang Feng Duan Xu Zhu Yu-Ting Wang Xu-Yi Chen Sai Zhang | 2019 | Neural Regeneration Research2019，14，6： | 1 |
| 15 | Study of surfactant level in cases of primary atrophic rhinitis显示文摘 | Sayed RH Abou-Elhamd KE Abdel-Kader M | 2000 | J Laryngol Otol2000，114，4： | 1 |
| 16 | Retinol binding protein 4 promotes hyperinsulinism?induced proliferationof rat aortic smooth muscle cells显示文摘 | Fei Li Ke Xia Md Sayed Sheikh Jinfang Cheng Chuanchang Li Tianlun Yang | 2014 | Molecular Medicine Reports2014，，5： | 1 |
| 17 | Development and evaluation of a predictive nomogram for survival in heat stroke patients: a retrospective cohort study显示文摘BACKGROUND:This study aimed to establish an eff ective nomogram to predict the survival of heat stroke(HS)based on risk factors.METHODS:This was a retrospective,observational multicenter cohort study.We analyzed patients diagnosed with HS,who were treated between May 1 and September 30,2018 at 15 tertiary hospitals from 11 cities in Northern China.RESULTS:Among the 175 patients,32 patients(18.29%)died before hospital discharge.After the univariate analysis,mechanical ventilation,initial mean arterial pressure<70 mmHg,maximum heart rate,lab results on day 1(white blood cell count,alanine aminotransferase,creatinine),and Glasgow admission prediction score were included in multivariate analysis.Multivariate Cox regression showed that invasive ventilation,initial mean arterial pressure<70 mmHg(1 mmHg=0.133 kPa),and Glasgow admission prediction score were independent risk factors for HS.The nomogram was established for predicting 7-d and 14-d survival in the training cohort.The nomogram exhibited a concordance index(C-index)of 0.880(95%confidence interval[95%CI]0.831-0.930)by bootstrapping validation(B=1,000).Furthermore,the nomogram performed better when predicting 14-d survival,compared to 7-d survival.The prognostic index cut-off value was set at 2.085,according to the operating characteristic curve for overall survival prediction.The model showed good calibration ability in the internal and external validation datasets.CONCLUSION:A novel nomogram,integrated with prognostic factors,was proposed;it was highly predictive of the survival in HS patients. | Fei Shao Xian Shi Shu-hua Huo Qing-yu Liu Ji-xue Shi Jian Kang Ping Gong Sheng-tao Yan Guo-xing Wang Li-jie Qin Fei Wang Ke Feng Feng-ying Chen Yong-jie Yin Tao Ma Yan Li Yang Wu Hao Cui Chang-xiao Yu Song Yang Wei Gan Sai Wang Liu-ye-zi Du Ming-chen Zhao Zi-ren Tang Shen Zhao | 2022 | World Journal of Emergency Medicine2022，13，5： | 1 |
| 18 | Dihydroartemisinin increases temozolomide efficacy in glioma cells byinducing autophagy显示文摘 | Ze?Shun Zhang Jing Wang You?Bi Shen Cheng?Cheng Guo Ke Sai Fu?Rong Chen Xin Mei Fu Han Zhong?Ping Chen | 2015 | Oncology Letters2015，，1： | 1 |
| 19 | cAMP-PGC1α途径诱导的抗Warburg效应驱动胶质母细胞瘤细胞分化为星形胶质细胞显示文摘文章简介多形性胶质母细胞瘤(GBM)是人类最具侵袭性的肿瘤之一。虽然分化治疗已被提出作为治疗GBM的潜在方法,但诱导分化的机制仍然很不清楚。在此,课题组利用c AMP激活剂建立了GBM诱导分化模型,该模型特异性地指导GBM分化为星形胶质细胞。 | Fan Xing Yizhao Luan Jing Cai Sihan Wu Jialuo Mai Jiayu Gu Haipeng Zhang Kai Li Yuan Lin Xiao Xiao Jiankai Liang Yuan Li Wenli Chen Yaqian Tan Longxiang Sheng Bingzheng Lu Wanjun Lu Mingshi Gao Pengxin Qiu Xingwen Su Wei Yin Jun Hu Zhongping Chen Ke Sai Jing Wang Furong Chen Yinsheng Chen Shida Zhu Dongbing Liu Shiyuan Cheng 谢志 朱文博 颜光美 | 2018 | 科学新闻2018，0，4： | 1 |
| 20 | Factors affecting rate of stroke-related death in elderly male military population A 23-year cohort study of 1268 cases in Xi'an,China显示文摘The study is the first long-term cohort study examining stroke and its subtypes among a population of Chinese elderly male retired military veterans.We reported on a 23-year cohort study examining stroke in 1 268 elderly male patients living in Xi'an,China since 1987.The stroke-related mortality rate in this cohort was 361.50/1 × 106 per year.Cerebral hemorrhage was the dominant cause of death,with 28 cases of fatal cerebral infarction and 49 cases of fatal cerebral hemorrhage among 77 stroke-related deaths.Independent risk factors for stroke mortality included age,blood pressure,smoking,body mass index,family history of hypertension,past medical history of stroke,hypertension and hyperlipidemia.Among them,ischemic stroke mortality correlated with age,smoking,family history of hypertension and past medical history of stroke,while hemorrhagic stroke was related to blood pressure,body mass index and past medical history of hypertension.Our results indicated that maintaining appropriate levels of blood pressure and body mass,smoking cessation and prevention of hyperlipidemia can reduce the risk of stroke-related death in elderly males who are retired from military service. | Yanhua Li Yu Sun Xiaoyong Sai Yao He Qiang Wu Yongping Yan Ke Men Liangshou Li | 2011 | Neural Regeneration Research2011，6，13： | 0 |
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