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| 1 | Complete genome analysis of Ketogulonigenium sp. WB0104显示文摘Ketogulonigenium sp. may convert L-sorbose into 2-keto-L-gulonic acid, the vitamin C precursor. The genome of Ketogulonigenium sp. WB0104 consists of a circular 2765030 bp chromo- some with 61.69% G+C content and two circular plasmids of 267968 and 242707 bp. The genome contains 2727 open reading frames (ORFs). The systems of replication, transcription, translation, carbohydrate and energy metabolism are intact, but the repair system is incomplete. About 640 predicted ORFs have been found to encode transporter pro- teins, which account for about one fourth of total predicted ORFs, noticeably higher than other docu- mented bacteria. This may be due to the fact that WB0104 adapts to soil circumstance. | YANG Fan JIA Qian XIONG Zhaohui ZHANG Xiaobing WU Hongtao ZHAO Ying YANG Jian ZHU Junping DONG Jie XUE Ying SUN Lilian SHEN Yan JIN Qi | 2006 | Chinese Science Bulletin2006，51，8： | 6 |
| 2 | Characterization of viromes within mosquito species in China显示文摘Mosquitoes are vectors of multiple disease-causing agents that cause worldwide outbreaks of serious infectious diseases in both humans and animals. These arboviral agents include Dengue virus (DENV), Zika virus (ZIKV), chikungunya virus, yellow fever virus, Japanese encephalitis virus (JEV),and Rift Valley fever virus. Thus, mosquitoes are key to the study of viral origin, spread, and the epidemic tendency of these infectious diseases (Hall and Macdonald, 2016). | Jiang Du Fan Li Yelin Han Shihong Fu Bo Liu Nan Shao Haoxiang Su Weijia Zhang Dandan Zheng Wenwen Lei Jie Dong Lilian Sun Ying He Jianmin Wang Fan Yang Huanyu Wang Guodong Liang Zhiqiang Wu Qi Jin | 2020 | Science China(Life Sciences)2020，63，7： | 2 |
| 3 | Construction, detection and microarray analysis on the Shigella flexneri 2a sitC mutant显示文摘In order to overcome the defects of difficult gene operations in low-copy suicide plasmid pCVD442, Gateway technology was applied in the construction process of recombinant plasmid for gene knockout in this study. With this improved knockout system, we inactivated sitC gene, which is associated with iron transport in Shigella flexneri 2a strain 301, to yield the mutant, MTS. The functional detection of the mutant was performed at the level of culture medium, cell and animal experiment, respectively. The gene expression profiles were compared with DNA microarray between the mutant and the wild type under iron-restricted conditions. The results showed that MTS grew obviously less well than the wild-type strains in L broth containing 150 μmol/L iron chelator DIP(2,2′-dipyridyl). Addition of iron or manganese to the cultures stimulated the growth of MTS to wild-type levels in rich culture medium. In either the experiment on the ability of intracellular multiplication and cell-to-cell spread in HeLa and U937 cell lines, or the experiment on keratoconjunctivitis in guinea pigs, MTS showed no obvious changes in virulence compared with the parental strain Sf301. When 65 μmol/L DIP was added to the cultured HeLa cells, the ability of intracellular multiplication of MTS reduced about 51.6% as compared with that of Sf301. The analysis of expression profiles under iron-limited condition showed that MTS was more sensitive for the change of iron deficiency than Sf301. There are 106 more up-regulated genes in MTS than in wild-type strains, which are involved in membrane transportation, amino acid metabolism and uncategorized function genes, while down-regulated genes are mainly in- volved in energy and carbohydrate metabolism. Under low iron conditions, the expression levels of known iron-transport associated genes generally increased. Additionally, the number of these genes and their increase amplitude in MTS are more than those in Sf301. Together, these results confirmed that Sit iron-transport system is important for the growth of Shigella. | LIU Moqing LIU Hong SUN Lilian DONG Jie XUE Ying CHEN Shuxia JIN Qi | 2005 | Science China(Life Sciences)2005，48，3： | 1 |
| 4 | Construction, detection and microarray analysis on Shigella dysenteriae A1 IroN, ShuA single, double mutants显示文摘In this study, we constructed single mutants MTS-1, MTS-2 of IroN and ShuA gene and double mutant MTS of them in Shigella dysenteriae A1 strain 51197 by insert and absence. The functional detection of every mutant was performed at the level of culture medium and cell experiment. The gene expression profiles of the mutants and the wild-type strains under iron- enriched and iron-limited conditions were analyzed by the SD51197 whole genomic microarray. The results showed that all the mutants grew obviously less well than the wild-type strains in L broth appending iron chelator DIP. The addition of iron to the cultures can stimulate the growth of mutants back to wild-type levels. In either the experiments on the ability of intracellular multiplication or the cell-to-cell spread in HeLa and U937 cell lines, mutants showed no obvious change in virulence compared with the parental strain SD51197. However when DIP was added to the cultured HeLa cells, the ability of intracellular multiplication of MTS-1, MTS-2, MTS has reduced about 23.4%, 25.2%, 43.6% respectively. The analysis of expression profiles under the iron-limited condition showed that the mutants were more sensitive for the changes of iron deficiency than the wild-type strains, many genes have been altered. Up-regulated genes mainly involved genes of transcription, coenzyme metabolism, amino acid transport and metabolism, and unknown functional genes, while down-regulated genes mainly involved genes of energy and carbohydrate metabolism and unknown function genes; the expression levels of known iron-transport associated genes generally showed up-regulated. The results demonstrated that iron-transport associated genes IroN, ShuA were likely to have some effects on the virulence and growth of S. dysenteriae. | BIN Wen, LIU Moqing, PENG Junping, SUN Lilian, XU Xingye, ZHANG Jinghai& JIN Qi Shengyang Pharmaceutical University, School of Pharmaceutical Engineering, Shengyang 110016, China State Key Laboratory for Molecular Virology and Genetic Engineering, Beijing 100176, China | 2006 | Science China(Life Sciences)2006，49，3： | 1 |
| 5 | Distinct lung microbial community states in patients with pulmonary tuberculosis显示文摘An improved understanding of the lung microbiome may lead to better strategies to diagnose, treat, and prevent pulmonary tuberculosis (PTB). However, the characteristics of the lung microbiomes of patients with TB remain largely undefined. In this study, 163 bronchoalveolar lavage (BAL) samples were collected from 163 sputum-negative suspected PTB patients. Furthermore, 12 paired BAL samples were obtained from 12 Mycobacterium tuberculosis-positive (MTB+) patients before and after negative conversion following a two-month anti-TB treatment. The V3–V4 region of the 16S ribosomal RNA (rRNA) gene was used to characterize the microbial composition of the lungs. The results showed that the prevalence of MTB in the BAL samples was 42.9% (70/163) among the sputum-negative patients. The α-diversity of lung microbiota was significantly less diverse in MTB+ patients compared with Mycobacterium tuberculosis-negative (MTB–) patients. There was a significant difference in β-diversity between MTB+ and MTB– patients. MTB+ patients were enriched with Anoxybacillus, while MTB– patients were enriched with Prevotella, Alloprevotella, Veillonella, and Gemella. There was no significant difference between the Anoxybacillus detection rates of MTB+ and MTB– patients. The paired comparison between the BAL samples from MTB+ patients and their negative conversion showed that BAL negative-conversion microbiota had a higher α-diversity. In conclusion, distinct features of airway microbiota could be identified between samples from patients with and without MTB. Our results imply links between lung microbiota and different clinical groups of active PTB. | Yongfeng Hu Ying Kang Xi Liu Min Cheng Jie Dong Lilian Sun Yafang Zhu Xianwen Ren Qianting Yang Xinchun Chen Qi Jin Fan Yang | 2020 | Science China(Life Sciences)2020，63，10： | 1 |
| 6 | Genetic diversity of coronaviruses in Miniopterus fuliginosus bats显示文摘Coronaviruses, such as severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, pose significant public health threats. Bats have been suggested to act as natural reservoirs for both these viruses, and periodic monitoring of coronaviruses in bats may thus provide important clues about emergent infectious viruses. The Eastern bent-wing bat Miniopterus fuliginosus is distributed extensively throughout China. We therefore analyzed the genetic diversity of coronaviruses in samples of M. fuliginosus collected from nine Chinese provinces during 2011–2013. The only coronavirus genus found was Alphacoronavirus. We established six complete and five partial genomic sequences of alphacoronaviruses, which revealed that they could be divided into two distinct lineages, with close relationships to coronaviruses in Miniopterus magnater and Miniopterus pusillus. Recombination was confirmed by detecting putative breakpoints of Lineage 1 coronaviruses in M. fuliginosus and M. pusillus(Wu et al., 2015), which supported the results of topological and phylogenetic analyses. The established alphacoronavirus genome sequences showed high similarity to other alphacoronaviruses found in other Miniopterus species, suggesting that their transmission in different Miniopterus species may provide opportunities for recombination with different alphacoronaviruses. The genetic information for these novel alphacoronaviruses will improve our understanding of the evolution and genetic diversity of coronaviruses, with potentially important implications for the transmission of human diseases. | Jiang Du Li Yang Xianwen Ren Junpeng Zhang Jie Dong Lilian Sun Yafang Zhu Fan Yang Shuyi Zhang Zhiqiang Wu Qi Jin | 2016 | Science China(Life Sciences)2016，59，6： | 1 |
| 7 | Reprogramming immunosuppressive myeloid cells by activated T cells promotes the response to anti-PD-1 therapy in colorectal cancer显示文摘Overcoming local immunosuppression is critical for immunotherapy to produce robust anti-tumor responses.Myeloid-derived suppressor cells(MDSCS)are key regulators of immunosuppressive networks and promote tumor progression.However,it remains unclear whether and how tumor-infltrating MDSCS are shaped in response to anti-PD-1 treatment and what their impact on therapeutic efficacy is in colorectal cancer(CRC).In this study,the levels of infltrating MDSCS were significantly higher in the non-responding organoids and were selectively reduced in the responding group,with MDSCS showing increased apoptosis and attenuated functional activity after anti-PD-1 treatment.A negative correlation between T-cell activation and MDSC function was also observed in fresh human CRC tissues.Mechanistic studies revealed that autocrine IFN-α/B upregulated TRAIL expression on activated T cells to elicit MDSC apoptosis via the TRAIL-DR5 interaction and acted synergistically with TNF-α to inhibit MDSC function of suppressing the T-cell response through the JNK NMDAR-ARG-1 pathway.Moreover,blockade of IFNa/βand TNF-α abolished the therapeutic efficacy of anti-PD-1 treatment by preserving the frequency and suppressive activity of infltrating MDSCS in a CRC mouse model.This result suggested that reprogramming MDSCS by IFN-α/βand TNF-α from activated T cells was necessary for succesful anti-PD-1 treatment and might serve as a novel strategy to improve the response and efficacy of anticancer therapy. | Jing Chen Hong-Wei Sun Yan-Yan Yang Hai-Tian Chen Xing Juan Yu Wen Chao Wu Yi-Tuo Xu LiLian Jin Xiao-Jun Wu Jing Xu Limin Zheng | 2021 | Signal Transduction and Targeted Therapy2021，6，2： | 0 |
| 8 | Image encryption based on a novel memristive chaotic system,Grain-128a algorithm and dynamic pixel masking显示文摘In this paper,we first propose a memristive chaotic system and implement it by circuit simulation.The chaotic dynamics and various attractors are analysed by using phase portrait,bifurcation diagram,and Lyapunov exponents.In particular,the system has robust chaos in a wide parameter range and the initial value space,which is favourable to the security communication application.Consequently,we further explore its application in image encryption and present a new scheme.Before image processing,the external key is protected by the Grain-128a algorithm and the initial values of the memristive system are updated with the plain image.We not only perform random pixel extraction and masking with the chaotic cipher,but also use them as control parameters for Brownian motion to obtain the permutation matrix.In addition,multiplication on the finite field GF(2^(8))is added to further enhance the cryptography.Finally,the simulation results verify that the proposed image encryption scheme has better performance and higher security,which can effectively resist various attacks. | HUANG Lilian SUN Yi XIANG Jianhong WANG Linyu | 2022 | Journal of Systems Engineering and Electronics2022，33，3： | 0 |
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