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| 1 | Two RING-Finger Ubiquitin E3 Ligases Regulate the Degradation of SPX4, An Internal Phosphate Sensor, for Phosphate Homeostasis and Signaling in Rice显示文摘SPX-domain-containing proteins (SPXs) play an important role in inorganic phosphate (Pi) sensing,signaling,and transport in eukaryotes.In plants,SPXs are known to integrate cellular Pi status and negatively regulate the activity of Pi central regulators,the PHOSPATE STARVATION RESPONSE proteins (PHRs).The stability of SPXs,such as SPX4,is reduced under Pi-deficient conditions.However,the mechanisms by which SPXs are degraded remain unclear.In this study,using a yeast-twhybrid screen we iden.tified two RING-finger ubiquitin E3 ligases regulating SPX4 degradation,designated SDEL1 and SDEL2,which were post-transcriptionally induced by Pi starvation.We found that both SDELs were located in the nucleus and cytoplasm,had ubiquitin E3 ligase activity,and directly ubiquitinated the K^213 and K^299 lysine residues in SPX4 to regulate its stability.Furthermore,we found that PHR2,a Pi central regulator in rice,could compete with SDELs by interacting with SPX4 under Pi-sufficient conditions,which protected SPX4 from ubiquitination and degradation.Consistent with the biochemical function of SDEL1 and SDEL2,overexpression of SDEL1 or SDEL2 resulted in Pi overaccumulation and induced Pi-starvation signaling even under Pi-sufficient conditions.Conversely,their loss-of-function mutants displayed decreased Pi accumulation and reduced Pi-starvation signaling.Collectively,our study revealed that SDEL1 and SDEL2 facilitate the degradation of SPX4 to modulate PHR2 activity and regulate Pi homeostasis and Pi signaling in response to external Pi availability in rice. | Wenyuan Ruan Meina Guo Xueqing Wang Zhenhui Guo Zhuang Xu Lei Xu Hongyu Zhao Haiji Sun Chengqi Yan Keke Yi | 2019 | Molecular Plant2019，12，8： | 14 |
| 2 | Cap-specific, terminal N^6-methylation by a mammalian m6Am methyltransferase显示文摘Dear Editor,Dynamic and reversible N6-methyladenosine (m6A) RNA methylation has been found to greatly impact gene expression, leading to the field of epitranscriptomics.1 Unlike m6A that is an internal modification, a terminal modification at mRNA cap in higher eukaryotes exists, termed as N6,2′-O-dimethyladenosine (m6Am) (Fig. 1a). | Hanxiao Sun Meiling Zhang Kai Li Dongsheng Bai Chengqi Yi | 2019 | Cell Research2019，29，1： | 7 |
| 3 | Effects of intermittent loading time and stress ratio on dwell fatigue behavior of titanium alloy Ti-6Al-4V ELI used in deep-sea submersibles显示文摘Different components of deep-sea submersibles,such as the pressure hull,are usually subjected to intermittent loading,dwell loading,and unloading during service.Therefore,for the design and reliability assessment of structural parts under dwell fatigue loading,understanding the effects of intermittent loading time on dwell fatigue behavior of the alloys is essential.In this study,the effects of the intermittent loading time and stress ratio on dwell fatigue behavior of the titanium alloy Ti-6 Al-4 V ELI were investigated.Results suggest that the dwell fatigue failure modes of Ti-6 Al-4 V ELI can be classified into three types,i.e.,fatigue failure mode,ductile failure mode,and mixed failure mode.The intermittent loading time does not affect the dwell fatigue behavior,whereas the stress ratio significantly affects the dwell fatigue life and dwell fatigue mechanism.The dwell fatigue life increases with an increase in the stress ratio for the same maximum stress,and specimens with a negative stress ratio tend to undergo ductile failure.The mechanism of dwell fatigue of titanium alloys is attribute to an increase in the plastic strain caused by the part of the dwell loading,thereby resulting in an increase in the actual stress of the specimens during the subsequent loading cycles and aiding the growth of the formed crack or damage,along with the local plastic strain or damage induced by the part of the fatigue load promoting the cumulative plastic strain during the dwell fatigue process.The interaction between dwell loading and fatigue loading accelerates specimen failure,in contrast to the case for individual creep or fatigue loading alone.The dwell fatigue life and cumulative maximum strain during the first loading cycle could be correlated by a linear relationship on the log–log scale.This relationship can be used to evaluate the dwell fatigue life of Ti alloys with the maximum stress dwell. | Chengqi Sun Yanqing Li Kuilong Xu Baotong Xu | 2021 | Journal of Materials Science & Technology2021，，18： | 7 |
| 4 | Improvement in grain refinement efficiency of Mg-Zr master alloy for magnesium alloy by friction stir processing显示文摘Previous studies have proved that the zirconium(Zr)alloying and grain refining performance of a Mg-Zr master alloy on Mg alloy is closely related to the distribution of Zr particle size,and a Mg-Zr master alloy with more Zr particles in size range of 1-5μm exhibits a better refining efficiency.In this paper,friction stir processing(FSP)was used to modify the Zr particles size distribution of a commercially available Mg-30 wt.%Zr master alloy,and the subsequent grain refinement ability was studied by trials on a typical Mg-3Nd-0.2Zn-0.6Zr(wt.%,NZ30K)alloy.It is found that plenty of large Zr particles in the as-received Mg-30%Zr master alloy are broken by FSP.Grain refinement tests reveal that the refining efficiency of Mg-30%Zr alloy is significantly improved by FSP,which is attributed to the better distribution of Zr particles.The refinement effect by adding 0.6%FSP-ed Mg-30%Zr is approximately equivalent to that by adding 1.0%as-received Mg-30%Zr.Due to the easy and convenient operation of FSP,this study provides a new method to develop a more efficient Mg-Zr refiner. | Chengqi Wang Ming Sun Feiyan Zheng Liming Peng Wenjiang Ding | 2014 | Journal of Magnesium and Alloys2014，2，3： | 7 |
| 5 | A two-parameter model to predict fatigue life of high-strength steels in a very high cycle fatigue regime显示文摘In this paper, ultrasonic(20 kHz) fatigue tests were performed on specimens of a high-strength steel in very high cycle fatigue(VHCF) regime. Experimental results showed that for most tested specimens failed in a VHCF regime, a fatigue crack originated from the interior of specimen with a fish-eye pattern, which contained a fine granular area(FGA) centered by an inclusion as the crack origin. Then,a two-parameter model is proposed to predict the fatigue life of high-strength steels with fish-eye mode failure in a VHCF regime, which takes into account the inclusion size and the FGA size. The model was verified by the data of present experiments and those in the literature. Furthermore, an analytic formula was obtained for estimating the equivalent crack growth rate within the FGA. The results also indicated that the stress intensity factor range at the front of the FGA varies within a small range, which is irrespective of stress amplitude and fatigue life. | Chengqi Sun Xiaolong Liu Youshi Hong | 2015 | Acta Mechanica Sinica2015，31，3： | 6 |
| 6 | The m^(6)A methylome of SARS-CoV-2 in host cells显示文摘The newly identified Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)has resulted in a global health emergency because of its rapid spread and high mortality.The molecular mechanism of interaction between host and viral genomic RNA is yet unclear.We demonstrate herein that SARS-CoV-2 genomic RNA,as well as the negative-sense RNA,is dynamically N6-methyladenosine(m^(6)A)-modified in human and monkey cells.Combined RIP-seq and miCLIP analyses identified a total of 8 m^(6)A sites at single-base resolution in the genome.Especially,epidemic strains with mutations at these identified m^(6)A sites have emerged worldwide,and formed a unique cluster in the US as indicated by phylogenetic analysis.Further functional experiments showed that m^(6)A methylation negatively regulates SARS-CoV-2 infection.SARS-CoV-2 infection also triggered a global increase in host m^(6)A methylome,exhibiting altered localization and motifs of m^(6)A methylation in mRNAs.Altogether,our results identify m^(6)A as a dynamic epitranscriptomic mark mediating the virus–host interaction. | Jun’e Liu Yan-Peng Xu Kai Li Qing Ye Hang-Yu Zhou Hanxiao Sun Xiaoyu Li Liu Yu Yong-Qiang Deng Rui-Ting Li Meng-Li Cheng Bo He Jia Zhou Xiao-Feng Li Aiping Wu Chengqi Yi Cheng-Feng Qin | 2021 | Cell Research2021，31，4： | 5 |
| 7 | Dynamic torsional buckling of multi-walled carbon nanotubes embedded in an elastic medium显示文摘在这篇论文在有弹性的媒介嵌入的多围的碳 nanotubes (MWNT ) 的动态扭力的弄弯被使用一个连续统力学模型学习。由为 MWNT 介绍起始的瑕疵并且使用比较喜欢的模式分析方法,一个弄弯的条件为弄弯的负担被导出并且联系了弄弯模式。特别地,明确的表情为嵌入的双 walled 碳 nanotubes (DWNT ) 被获得。为 DWNT 和嵌入的 DWNT,弄弯的形式与增加弄弯的负担从更低的弄弯模式转移到更高的弄弯模式的数字结果表演,而是弄弯的模式为弄弯的负担的某个领域是不变的。这也被显示,包围有弹性的媒介通常在 DWNT 的更低的弄弯模式上有效果仅仅与为单个 DWNT 的相应的相比。 | Chengqi Sun Kaixin Liu Guoxing Lu | 2008 | Acta Mechanica Sinica2008，24，5： | 4 |
| 8 | Effects of loading condition on very-high-cycle fatigue behaviour and dominant variable analysis显示文摘The specimens of a high carbon chromium steel were quenched and tempered at 150°C,180°C and 300°C.Such specimens were tested via rotating bending and a push-pull type of axial loading to investigate the influences of loading condition on the behaviour of very-high-cycle fatigue(VHCF).Experimental results show the different influences of inclusion size on the fatigue life for the two loading conditions.Predominant factors and mechanism for the fine-granular-area(FGA)of crack origin were discussed.In addition,a reliability analysis based on a modified Tanaka-Mura model was carried out to evaluate the sensitivity of inclusion size,stress,and KFGA to the life of VHCF crack initiation. | LEI ZhengQiang XIE JiJia SUN ChengQi HONG YouShi | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，1： | 4 |
| 9 | Effects of the Nozzle Design on the Properties of Plasma Jet and Formation of YSZ Coatings under Low Pressure Conditions显示文摘How to control the quality of the coatings has become a major problem during the plasma spraying. Because nozzle contour has a great influence on the characteristic of the plasma jet, two kinds of plasma torches equipped with a standard cylindrical nozzle and a converging-diverging nozzle are designed for low pressure plasma spraying(LPPS) and very low pressure plasma spraying(VLPPS). Yttria stabilized zirconia(YSZ) coatings are obtained in the reducing pressure environment. The properties of the plasma jet without or with powder injection are analyzed by optical emission spectroscopy, and the electron temperature is calculated based on the ratio of the relative intensity of two ArⅠspectral lines. The results show that some of the YSZ powder can be vaporized in the low pressure enlarged plasma jet, and the long anode nozzle may improve the characteristics of the plasma jet. The coatings deposited by LPPS are mainly composed of the equiaxed grains and while the unmelted powder particles and large scalar pores appear in the coatings made by VLPPS. The long anode nozzle could improve the melting of the powders and deposition efficiency, and enhance the coatings' hardness. At the same time, the long anode nozzle could lead to a decrease in the overspray phenomenon. Through the comparison of the two different size's nozzle, the long anode is much more suitable for making the YSZ coatings. | SUN Chengqi GAO Yang YANG Deming FU Yingqing | 2016 | Chinese Journal of Mechanical Engineering2016，29，5： | 3 |
| 10 | DYNAMIC BUCKLING OF DOUBLE-WALLED CARBON NANOTUBES UNDER STEP AXIAL LOAD显示文摘An approximate method is presented in this paper for studying the dynamic buckling of double-walled carbon nanotubes (DWNTs) under step axial load. The analysis is based on the continuum mechanics model,which takes into account the van der Waals interaction between the outer and inner nanotubes. A buckling condition is derived,from which the critical buckling load and associated buckling mode can be determined. As examples,numerical results are worked out for DWNTs under fixed boundary conditions. It is shown that,due to the effect of van der Waals forces,the critical buckling load of a DWNT is enhanced when inserting an inner tube into a single-walled one. The paper indicates that the critical buckling load of DWNTs for dynamic buckling is higher than that for static buckling. The effect of the radii is also examined. In addition,some of the results are compared with the previous ones. | Chengqi Sun Kaixin Liu | 2009 | Acta Mechanica Solida Sinica2009，22，1： | 2 |
| 11 | High-temperature failure mechanism and defect sensitivity of TC17 titanium alloy in high cycle fatigue显示文摘Crack initiation is an essential stage of fatigue process due to its direct effect on fatigue failure.However,for titanium alloys in high-temperature high cycle fatigue(HCF),the crack initiation mechanisms remain unclear and the understanding for the defect sensitivity is also lacking.In this study,a series of fatigue tests and multi-scale microstructure characterizations were conducted to explore the high-temperature failure mechanism,and the coupled effect of temperature and defect on TC17 titanium alloy in HCF.It was found that an oxygen-rich layer(ORL)was produced at specimen surface at elevated temperatures,and brittle fracture of ORL at surface played a critical role for surface crack initiation in HCF.Besides,internal crack initiation with nanograins at high temperatures was a novel finding for the titanium alloy.Based on energy dispersive spectroscopy,electron backscatter diffraction and transmission electron microscope characterizations,the competition between surface and internal crack initiations at high temperatures was related to ORL at surface and dislocation resistance in inner microstructure.The fatigue strengths of smooth specimens decreased at elevated temperatures due to the lower dislocation resistance.While the fatigue strengths of the specimens with defect were not very sensitive to the temperatures.Finally,a fatigue strength model considering the coupled effect of temperature and defect was proposed for TC17titanium alloy. | Gen Li Chengqi Sun | 2022 | Journal of Materials Science & Technology2022，，27： | 2 |
| 12 | Correlation of crack growth rate and stress ratio for fatigue damage containing very high cycle fatigue regime显示文摘A model is proposed to correlate the crack growth rate and stress ratio containing very high cycle fatigue regime.The model is verified by the experimental data in literature.Then a formula is derived for the effect of mean stress on fatigue strength,and it is used to estimate the fatigue strength of a bearing steel in very high cycle fatigue regime at different stress ratios.The estimated results are also compared with those by Goodman formula. | Chengqi Sun Youshi Hong | 2012 | Theoretical & Applied Mechanics Letters2012，2，3： | 2 |
| 13 | Effects of strength level and loading frequency on very-high-cycle fatigue be- havior for a bearing steel 显示文摘 | Zhao Aiguo Xie Jia Sun Chengqi | 2012 | International Journal of Fa- tigue2012，38，0： | 1 |
| 14 | Effects of strength level and loading frequency on very-high-cycle fatigue behavior for a bearing steel显示文摘 | Aiguo Zhao Jijia Xie Chengqi Sun Zhengqiang Lei Youshi Hong | 2011 | International Journal of Fatigue2011，，： | 1 |
| 15 | Effects of Strength Level and Loading Frequency on Very-High-Cycle Fatigue Be- havior for a Bearing Steel显示文摘 | Zhao Aiguo Xie Jijia Sun Chengqi | 2012 | International Journal of Fatigue2012，38，： | 1 |
| 16 | Prediction of threshold value for FGA formation显示文摘 | Aiguo Zhao Jijia Xie Chengqi Sun Zhengqiang Lei Youshi Hong | 2011 | Materials Science & Engineering A2011，，22： | 1 |
| 17 | DOCK2 regulates antifungal immunity by regulating RAC GTPase activity显示文摘Fungal infections cause~1.5 million deaths each year worldwide,and the mortality rate of disseminated candidiasis currently exceeds that of breast cancer and malaria.The major reasons for the high mortality of candidiasis are the limited number of antifungal drugs and the emergence of drug-resistant species.Therefore,a better understanding of antifungal host defense mechanisms is crucial for the development of effective preventive and therapeutic strategies.Here,we report that DOCK2(dedicator of cytokinesis 2)promotes indispensable antifungal innate immune signaling and proinflammatory gene expression in macrophages.DOCK2-deficient macrophages exhibit decreased RAC GTPase(Rac family small GTPase)activation and ROS(reactive oxygen species)production,which in turn attenuates the killing of intracellular fungi and the activation of downstream signaling pathways.Mechanistically,after fungal stimulation,activated SYK(spleen-associated tyrosine kinase)phosphorylates DOCK2 at tyrosine 985 and 1405,which promotes the recruitment and activation of RAC GTPases and then increases ROS production and downstream signaling activation.Importantly,nanoparticle-mediated delivery of in vitro transcribed(IVT)Rac1 mRNA promotes the activity of Rac1 and helps to eliminate fungal infection in vivo.Taken together,this study not only identifies a critical role of DOCK2 in antifungal immunity via regulation of RAC GTPase activity but also provides proof of concept for the treatment of invasive fungal infections by using IVT mRNA. | Xiaojian Ma Xi Tan Bingbing Yu Wanwei Sun Heping Wang Huijun Hu Yanyun Du Ruirui He Ru Gao Qianwen Peng Zhihui Cui Ting Pan Xiong Feng Junhan Wang Chengqi Xu Bin Zhu Wei Liu Chenhui Wang | 2022 | Cellular & Molecular Immunology2022，19，5： | 1 |
| 18 | Effects of Strength Level and Loading Frequency on Very-High-Cycle Fatigue Behavior for a Bearing Steel显示文摘 | ZHAO Aiguo XIE Jijia SUN Chengqi | | 0，，： | 1 |
| 19 | Dynamic shell buckling behavior of multi-walled carbon nanotubes embedded in an elastic medium显示文摘This paper studies the dynamic shell buckling behavior of multi-walled carbon nanotubes(MWNTs) embedded in an elastic medium under step axial load based on continuum mechanics model.It is shown that,for occurrence of dynamic shell buckling of MWNTs or MWNTs embedded in an elastic medium,the buckling stress is higher than the critical buckling stress of the corresponding static shell buckling under otherwise identical conditions.Detailed results are demonstrated for dynamic shell buckling of individual double-walled carbon nanotubes(DWNTs) or DWNTs embedded in an elastic medium.A phenomenon is shown that DWNTs or embedded DWNTs in dynamic shell buckling are prone to axisymmetric buckling rather than non-axisymmetric buckling.Numerical results also indicate that the axial buckling form shifts from the lower buckling mode to the higher buckling mode with increasing buckling stress,but the buckling mode is invariable for a certain domain of buckling stress.Further,an approximate analytic formula is presented for the buckling stress and the associated buckling wavelength for dynamic axisymmetric buckling of embedded DWNTs.The effect of radii is also examined. | SUN ChengQi LIU KaiXin HONG YouShi | 2013 | Science China Chemistry2013，56，3： | 1 |
| 20 | The Association Between H3K4me3 and Antisense Transcription显示文摘Histone H3 lysine 4 trimethylation(H3K4me3) is well known to occur in the promoter region of genes for transcription activation.However,when investigating the H3K4me3 profiles in the mouse cerebrum and testis,we discovered that H3K4me3 also has a significant enrichment at the 3 0 end of actively transcribed(sense) genes,named as 3 0-H3K4me3.3 0-H3K4me3 is associated with 15% of protein-coding genes in both tissues.In addition,we examined the transcriptional initiation signals including RNA polymerase II(RNAPII) binding sites and 5 0-CAGE-tag that marks transcriptional start sites.Interestingly,we found that 3 0-H3K4me3 is associated with the initiation of antisense transcription.Furthermore,3 0-H3K4me3 modification levels correlate positively with the antisense expression levels of the associated sense genes,implying that 3 0-H3K4me3 is involved in the activation of antisense transcription.Taken together,our findings suggest that H3K4me3 may be involved in the regulation of antisense transcription that initiates from the 3 0 end of sense genes.In addition,a positive correlation was also observed between the expression of antisense and the associated sense genes with 3 0-H3K4me3 modification.More importantly,we observed the 3 0-H3K4me3 enrichment among genes in human,fruitfly and Arabidopsis,and found that the sequences of 3 0-H3K4me3-marked regions are highly conserved and essentially indistinguishable from known promoters in vertebrate.Therefore,we speculate that these 3 0-H3K4me3-marked regions may serve as potential promoters for antisense transcription and 3 0-H3K4me3 appear to be a universal epigenetic feature in eukaryotes.Our results provide a novel insight into the epigenetic roles of H3K4me3 and the regulatory mechanism of antisense transcription. | Peng Cui Wanfei Liu Yuhui Zhao Qiang Lin Feng Ding Chengqi Xin Jianing Geng Shuhui Song Fanglin Sun Songnian Hu Jun Yu | 2012 | Genomics, Proteomics & Bioinformatics2012，10，2： | 1 |
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