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| 1 | Liver-targeted hydrodynamic gene therapy: Recent advances in the technique显示文摘One of the major research focuses in the field of gene therapy is the development of clinically applicable, safe, and effective gene-delivery methods. Since the first case of human gene therapy was performed in 1990, a number of gene-delivery methods have been developed, evaluated for efficacy and safety, and modified for human application. To date, viral-vectormediated deliveries have shown effective therapeutic results. However, the risk of lethal immune response and carcinogenesis have been reported, and it is still controversial to be applied as a standard therapeutic option. On the other hand, delivery methods for nonviral vector systems have been developed, extensively studied, and utilized in in vivo gene-transfer studies. Compared to viral-vector mediated gene transfer, nonviral systems have less risk of biological reactions. However, the lower gene-transfer efficiency was a critical hurdle for applying them to human gene therapy. Among a number of nonviral vector systems, our studies focus on hydrodynamic gene delivery to utilize physical force to deliver naked DNA into the cells in the living animals. This method achieves a high gene-transfer level by DNA solution injections into the tail vein of rodents, especially in the liver. With the development of genome editing methods, in vivo gene-transfer therapy using this method is currently the focus in this research field. This review explains the method principle, efficiency, safety, and procedural modifications to achieve a high level of reproducibility in large-animal models. | Takeshi Yokoo Kenya Kamimura Hiroyuki Abe Yuji Kobayashi Tsutomu Kanefuji Kohei Ogawa Ryo Goto Masafumi Oda Takeshi Suda Shuji Terai | 2016 | World Journal of Gastroenterology2016，22，40： | 3 |
| 2 | Autonomic nervous system network and liver regeneration显示文摘To date, various signal transducers, cytokines, growth factors, and hormones have been reported to play an important role in homeostasis of various organs. Various cells and organs are involved in the hepatic regeneration process, which proceeds as a result of the coordination of many factors. While these factors are well known to be involved in the liver regeneration after the liver injury, however, as the details of such mechanisms have not been sufficiently elucidated, the practical applicability of hepatic regeneration based on the action of these and cytokines growth factors is still unclear. In terms of the involvement of the autonomic nervous system in hepatic regeneration, cell proliferation resulting from direct signal transduction to the liver has also been reported and recent studies focusing on the inter-organ communication via neural network opened a novel aspect of this field for therapeutic applicability. Therefore, the appropriate understanding of the relationship between autonomic neural network and liver regeneration through various organs including brain, afferent nerve, efferent nerve, etc. is essential. This mini-review explains the principle of neural system involved in the inter-organ communication and its contribution on the liver regeneration upon the liver injury reviewing recent progress in this field. | Kenya Kamimura Ryosuke Inoue Takuro Nagoya Norihiro Sakai Ryo Goto Masayoshi Ko Yusuke Niwa Shuji Terai | 2018 | World Journal of Gastroenterology2018，24，15： | 3 |
| 3 | Hypersensitivity to Suture Anchors显示文摘 | Masafumi Goto Masafumi Gotoh Yasuhiro Mitsui Ryo Tanesue Takahiro Okawa Fujio Higuchi Naoto Shiba A. Nehme A. Ramasamy | 2013 | Case Reports in Orthopedics2013，，： | 1 |
| 4 | Study on Dynamic Response of Fiber-Reinforced Plastic Float for Light Seaplane显示文摘 | Yoshio Aoki Akihisa Tabata Ryo Goto Goichi Ben | 2016 | Journal of Civil Engineering and Architecture2016，10，2： | 0 |
| 5 | Deep learning-based magnetic resonance imaging reconstruction for improving the image quality of reduced-field-of-view diffusionweighted imaging of the pancreas显示文摘BACKGROUND It has been reported that deep learning-based reconstruction(DLR)can reduce image noise and artifacts,thereby improving the signal-to-noise ratio and image sharpness.However,no previous studies have evaluated the efficacy of DLR in improving image quality in reduced-field-of-view(reduced-FOV)diffusionweighted imaging(DWI)[field-of-view optimized and constrained undistorted single-shot(FOCUS)]of the pancreas.We hypothesized that a combination of these techniques would improve DWI image quality without prolonging the scan time but would influence the apparent diffusion coefficient calculation.AIM To evaluate the efficacy of DLR for image quality improvement of FOCUS of the pancreas.METHODS This was a retrospective study evaluated 37 patients with pancreatic cystic lesions who underwent magnetic resonance imaging between August 2021 and October 2021.We evaluated three types of FOCUS examinations:FOCUS with DLR(FOCUS-DLR+),FOCUS without DLR(FOCUS-DLR−),and conventional FOCUS(FOCUS-conv).The three types of FOCUS and their apparent diffusion coefficient(ADC)maps were compared qualitatively and quantitatively.RESULTS FOCUS-DLR+(3.62,average score of two radiologists)showed significantly better qualitative scores for image noise than FOCUS-DLR−(2.62)and FOCUS-conv(2.88)(P<0.05).Furthermore,FOCUS-DLR+showed the highest contrast ratio and 600 s/mm^(2)(0.72±0.08 and 0.68±0.08)and FOCUS-DLR−showed the highest CR between cystic lesions and the pancreatic parenchyma for the b-values of 0 and 600 s/mm2(0.62±0.21 and 0.62±0.21)(P<0.05),respectively.FOCUS-DLR+provided significantly higher ADCs of the pancreas and lesion(1.44±0.24 and 3.00±0.66)compared to FOCUS-DLR−(1.39±0.22 and 2.86±0.61)and significantly lower ADCs compared to FOCUS-conv(1.84±0.45 and 3.32±0.70)(P<0.05),respectively.CONCLUSION This study evaluated the efficacy of DLR for image quality improvement in reduced-FOV DWI of the pancreas.DLR can significantly denoise images without prolonging the scan time or decreasing the spatial resolution.The denoising level of DWI can be controlled to make the images appear more natural to the human eye.However,this study revealed that DLR did not ameliorate pancreatic distortion.Additionally,physicians should pay attention to the interpretation of ADCs after DLR application because ADCs are significantly changed by DLR. | Yukihisa Takayama Keisuke Sato Shinji Tanaka Ryo Murayama Nahoko Goto Kengo Yoshimitsu | 2023 | World Journal of Radiology2023，15，12： | 0 |
| 6 | Rapid detection of neutralizing antibodies to SARS-CoV-2 variants in post-vaccination sera显示文摘The uncontrolled spread of the coronavirus disease 2019(COVID-19)pandemic has led to the emergence of different severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants across the globe.The ongoing global vaccination strategy to curtail the COVID-19 juggernaut is threatened by the rapidly spreading variants of concern(VOC)and other regional mutants,which are less responsive to neutralization by infection-or vaccine-derived antibodies(Gomez et al.,2021;Wang et al.,2021). | Kei Miyakawa Sundararaj Stanleyraj Jeremiah Hideaki Kato Yutaro Yamaoka Hirofumi Go Satoshi Yajima Tomoko Shimada Takahiro Mihara Atsushi Goto Takeharu Yamanaka Akihide Ryo | 2021 | Journal of Molecular Cell Biology2021，13，12： | 0 |
| 7 | 虾青素摄入、热应激和间歇式再负荷的联合处理对废用性肌萎缩的肌卫星细胞的影响(英文)显示文摘目的:探讨透过虾青素摄入、热应激和间歇式再负荷的不同处理方式及其联合处理,对于尾部悬吊后大鼠比目鱼肌卫星细胞的影响。创新点:利用虾青素摄入、热应激与间歇式再负荷的不同方式,探讨预防大鼠比目鱼肌废用性肌萎缩的有效处理。方法:将49只雄性Wistar大鼠分成对照组、吊尾组、间歇式再负荷组、虾青素摄入组、热应激组和虾青素摄入+热应激组。摘取7天尾部悬吊后的大鼠的比目鱼肌,使用免疫组织化学技术,分析肌卫星细胞的激活程度。结论:虾青素摄入、热应激与间歇式再负荷的不同方式的联合处理能减少比目鱼肌废用性肌萎缩,其部分效果可能是由于肌卫星细胞数量的增加所致。 | Toshinori YOSHIHARA Takao SUGIURA Nobuyuki MIYAJI Yuki YAMAMOTO Tsubasa SHIBAGUCHI Ryo KAKIGI Hisashi NAITO Katsumasa GOTO Daijiro OHMORI Toshitada YOSHIOKA | 2018 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2018，19，11： | 0 |
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