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| 1 | One-step generation of knockout pigs by zygote injection of CRISPR/Cas system显示文摘 | Tang Hai Fei Teng Runfa Guo Wei Li Qi Zhou | 2014 | Cell Research2014，24，3： | 131 |
| 2 | Epigenetic reprogramming, gene expression and in vitro development of porcine SCNT embryos are significantly improved by a histone deacetylase inhibitor--- m-carboxycinnamic acid bishydroxamide (CBHA)显示文摘施主原子核的不够的 epigenetic reprogramming 被相信是哺乳动物的体的房间的低发展效率的最重要的原因之一原子转移(SCNT ) 。以前的研究两个都显示出那在里面 vitro 并且在老鼠 SCNT 的 vivo 开发,胚胎能被处理与各种各样的 histone deacetylase 禁止者( HDACi )显著地增加包括 Trichostatin A , Scriptaid ,和 m-carboxycinnamic 酸 bishydroxamide ( CBHA ),在哪个仅仅 CBHA 的效果还没在另外的种类被测试了。在这份报纸,我们在猪的 SCNT 胚胎的发展检验 CBHA 处理的效果。我们发现了为 CBHA 处理的最佳剂量和时间:在激活以后为 24 h 与 2 mol/L CBHA 孵化 SCNT 胚胎能从 12.7% ~ 26.5% 增加胚囊率。Immunofluorescence 结果证明在 histone 的 acetylation 的水平 3 离氨酸 9 (AcH3K9 ) ,在 histone 的 acetylation 3 离氨酸 18 (AcH3K18 ) ,并且在 histone 的 acetylation 4 离氨酸(AcH4K16 ) 16 在 CBHA 处理以后被提起。同时, CBHA 处理改进了联系象 pou5f1, cdx2,和象 igf2 一样的印的基因那样的基因的开发的表达式。尽管有这些在 vitro 结果和 histone reprogramming 答应,完整的术语发展显著地没在处理以后被增加。在结论, CBHA 改善在里面猪 SCNT 胚胎的 vitro 开发,增加全球 histone acetylation 并且在早阶段改正一些发展地重要的基因的表示。作为在老鼠 SCNT,我们早在猪显示出那原子 epigenetic reprogramming SCNT 胚胎能被 CBHA 处理修改。 | Yuran Song Tang Hai Ying Wang Runfa Guo Wei Li Liu Wang Qi Zhou | 2014 | Protein & Cell2014，5，5： | 9 |
| 3 | Homeobox B8 Targets Sterile Alpha Motif Domain-Containing Protein 9 and Drives Glioma Progression显示文摘Gliomas are the most commonly occurring tumors of the central nervous system. Glioblastoma multiforme (GBM) is the most malignant and aggressive brain cancer in adults. Further understanding of the mechanisms underlying the aggressive nature of GBM is urgently needed. Here we identified homeobox B8(HOXB8), a member of the homeobox family, as a crucial contributor to the aggressiveness of GBM. Data mining of publicly accessible RNA sequence datasets and our patient cohorts confirmed a higher expression of HOXB8 in the tumor tissue of GBM patients, and a strong positive correlation between the expression level and pathological grading of tumors and a negative correlation between the expression level and the overall survival rate. We next showed that HOXB8 promotes the proliferation and migration of glioblastoma cells and is crucial for the activation of the PI3K/AKT pathway and expression of epithelial–mesenchymal transition-related genes, possibly through direct binding to the promoter of SAMD9 (Sterile Alpha Motif Domain-Containing Protein 9) and activating its transcription. Collectively, we identified HOXB8 as a critical contributor to the aggressiveness of GBM, which provides insights into a potential therapeutic target for GBM and opens new avenues for improving its treatment outcome. | Wenping Ma Hongze Jin Wenjie Liu Xiaojuan Li Xingang Zhou Xinwu Guo Runfa Tian Qi Cui Junjie Luo Yueying Jiao Youtao Yu Haifeng Yang Hongshan Zhao | 2020 | Neuroscience Bulletin2020，36，4： | 2 |
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