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| 1 | Knockdown of MLO genes reduces susceptibility to powdery mildew in grapevine显示文摘Erysiphe necator is the causal agent of powdery mildew(PM),one of the most destructive diseases of grapevine.PM is controlled by sulfur-based and synthetic fungicides,which every year are dispersed into the environment.This is why PM-resistant varieties should become a priority for sustainable grapevine and wine production.PM resistance can be achieved in other crops by knocking out susceptibility S-genes,such as those residing at genetic loci known as MLO(Mildew Locus O).All MLO S-genes of dicots belong to the phylogenetic clade V,including grapevine genes VvMLO7,11 and 13,which are upregulated during PM infection,and VvMLO6,which is not upregulated.Before adopting a gene-editing approach to knockout candidate S-genes,the evidence that loss of function of MLO genes can reduce PM susceptibility is necessary.This paper reports the knockdown through RNA interference of VvMLO6,7,11 and 13.The knockdown of VvMLO6,11 and 13 did not decrease PM severity,whereas the knockdown of VvMLO7 in combination with VvMLO6 and VvMLO11 reduced PM severity up to 77%.The knockdown of VvMLO7 and VvMLO6 seemed to be important for PM resistance,whereas a role for VvMLO11 does not seem likely.Cell wall appositions(papillae)were present in both resistant and susceptible lines in response to PM attack.Thirteen genes involved in defense were less upregulated in infected mlo plants,highlighting the early mlo-dependent disruption of PM invasion. | Stefano Pessina Luisa Lenzi Michele Perazzolli Manuela Campa Lorenza Dalla Costa Simona Urso Giampiero Valè Francesco Salamini Riccardo Velasco Mickael Malnoy | 2016 | Horticulture Research2016，3，1： | 7 |
| 2 | Bcl-2 down modulation in WEHI-3B/CTRES cells resistant to Cholera Toxin(CT)-induced apoptosis显示文摘细胞生长和增长上的霍乱毒素(CT ) 的不同效果可以在细胞膜和触发的不同信号 transduction 机制取决于 ganglioside 受体的类型,但是其它与药抵抗机制有关工作不能被排除。CT 处理在上的效果“在 vitro ”, clonogenicity,人口双倍工资(太平洋夏季时间) , apoptosis, PKA 激活和 Bax 和 Bcl-2 表示在 WEHI-3B 房间线和它的 CT 抵抗的潜水艇克隆(WEHI-3B/CTRES ) 被评估。在 WEHI-3B 父母房间, CT 导致的营地的戏剧的累积与 PKA 激活相关很好,增加的太平洋夏季时间价值, clonogenicity 和 apoptosis 的抑制。H-89 处理由 CT 禁止了 PKA 激活但是没保护房间免受 apoptosis 和生长抑制的伤害。在 WEHI-3B/CTRES,营地的重要 CT 依赖的累积都没随 PKA 活动和太平洋夏季时间的任何增加发生。在 CT 抵抗房间(WEHI-3B/CTRES ) , Bcl-2 表示低由 CT 或药处理调整了(例如, ciprofloxacin, CPX ) 尽管这些房间被保护免受 CT 依赖的 apoptosis 的伤害然而并非从导致药的 apoptosis。不同地,从另外的房间,模型描述了,在 Bcl-2 的规定下面被证明在营地累积和 PKA 激活上独立。我们的观察在 WEHI-3B 细胞生长的抑制支持营地依赖者 kinase (PKA ) 的含意并且建议在 WEHI-3B/CTRES, Bcl-2 表示能被 CT 当营地累积不在时调制。另外,在许多的考虑,矛盾的数据在文学报导了,我们的房间模型(有到 CT 和 CPX 的不同使不交叉的特定的抵抗的一个敏感父母房间种类和二克隆)为更好调查在 CT 信号 transduction 机制和 Bcl-2 表示和函数之间的关系提供一个新、有趣的工具。 | Augusto Pessina Cristina Croera Nicoletta Savalli Arianna Bonomi Loredana Cavicchini Elisa Turlizzi Fabiana Guizzardi Lucia Guido Laura Daprai Maria GraziaNeri | 2006 | Cell Research2006，16，3： | 4 |
| 3 | Primary aldosteronism: an update on screening, diagnosis and treatment 显示文摘 | Rossi GP Pessina AC Heaqerty AM | 2008 | J Hypertens2008，26，4： | 1 |
| 4 | Primary aldosteronism: part Ⅱ: subtype differentiation and treatment显示文摘 | Rossi GP Seccia TM Pessina AC | 2008 | J Nephrol2008，21，： | 1 |
| 5 | Postnatal repeated maternal deprivation produces age-dependent changes of brain-derived neurotrophic factor expression in selected rat brain regions显示文摘 | Roceri M Cirulli F Pessina C | 2004 | Biol Psychiatry2004，55，： | 1 |
| 6 | Mesenchymal Stem/Stromal Cells: A New ’’Cells as Drugs’’ Paradigm. Efficacy and Critical Aspects in Cell Therapy显示文摘 | Laura de Girolamo Enrico Lucarelli Giulio Alessandri Maria Antonietta Avanzini Maria Ester Bernardo Ettore Biagi Anna Teresa Brini Giovanna D’Amico Franca Fagioli Ivana Ferrero Franco Locatelli Rita Maccario Mario Marazzi Ornella Parolini Augusto Pessina | 2013 | Current Pharmaceutical Design2013，，： | 1 |
| 7 | 8-week, single-blind, random- ized trial comparing risperidone versus olanzapine augmentation of serotonin reuptake inhibitors in treatment-resistant obsessive-com- pulsive disorder 显示文摘 | Maina G Pessina E Albert U | 2008 | Eur Neuropsychopharmacol2008，18，： | 1 |
| 8 | Pulsed electromagnetic fields affect the intracellular calcium concentrations in human astrocytoma cells显示文摘 | Pessina GP Aldinucci C Palmi M | 2001 | Bioelectromagnetics2001，22，7： | 1 |
| 9 | Low plasma adiponectin is associated with coronary artery disease but not with hypertension in high-risk nondiabetic patients显示文摘 | Cesari M Pessina A C Zanchetta M | | 0，，03： | 1 |
| 10 | In vitro toxicity of clozapine, olanzapine, and quetiapine on granulo- cyte-maerophage progenitors (GM-CFU) 显示文摘 | Pessina A Turlizzi E Bonomi A | 2006 | Pharmaco- psychiatry2006，39，1： | 1 |
| 11 | 显示文摘 | Pessina A Eletti B Croera C | 2004 | Biothem Biophys Res Commun2004，323，： | 1 |
| 12 | Tolerability of long-term treatment with lercanidipine versus amlodipine and lacidipine in elderly hypertensives显示文摘 | Leonetti G Magnani B Pessina AC | 2002 | AM J Hypertens2002，15，11： | 1 |
| 13 | 显示文摘 | Freddi G Pessina G Tsukada M | 1999 | Int J Bio Macro1999，24，： | 1 |
| 14 | A WebGis tool for seismic hazard scenarios and risk analysis显示文摘 | Vera Pessina Fabrizio Meroni | 2009 | Soil Dynamics and Earthquake Engineering2009，29，9： | 1 |
| 15 | A WebGIS tool for seismic hazard scenarios and risk analysis显示文摘 | Pessina V and Meroni F | 2009 | Soil Dynamics and Earthquake Engineering2009，29，9： | 1 |
| 16 | Biological properties of the angiotensin peptides other than angiotensin Ⅱ : implications for hypertension and cardiovascular diseases显示文摘 | Cesari M Rossi GP Pessina AC | 2002 | J Hypertens2002，20，5： | 1 |
| 17 | Role of intracellular Ca^2+ and ealmodulin/MAP kinase kinase/extracellular signal-regulated protein kinase signaling pathway in the mito genie and antimitogenic effect of nitric oxide in glia-and neurone derived cell lines 显示文摘 | Meini A Garcia JB Pessina GP | 2006 | Eur J Neurosei2006，23，： | 1 |
| 18 | Effects Of ovariectomy and steroid hormones on vaginal smooth muscle contractility显示文摘 | Kim N N Min K Pessina M A Munarriz R Goldstein l Traish A M | 2004 | Int J Impot Res2004，16，： | 1 |
| 19 | Sweooing and dissolution of silk fibroin (Bombyx mori) in N-methyl morpholineN-exide显示文摘 | Freddi G Pessina G Tsukada M | 1999 | Int J Bio Macro1999，24，23： | 1 |
| 20 | Aripiprazole augmentation of serotonin reuptake inhibitors in treatment-resistant obsessive- compulsive disorder: a 12-week open-label preliminary study显示文摘 | Pessina E Albert U Bogetto F | 2009 | Int Clin Fsychopharmacol2009，24，5： | 1 |
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