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| 1 | Modulation of faecal metagenome in Crohn's disease:Role of microRNAs as biomarkers显示文摘BACKGROUND The gut microbiota plays a key role in the maintenance of intestinal homeostasis and the development and activation of the host immune system. It has been shown that commensal bacterial species can regulate the expression of host genes. 16 S rRNA gene sequencing has shown that the microbiota in inflammatory bowel disease(IBD) is abnormal and characterized by reduced diversity. Micro RNAs(miRNAs) have been explored as biomarkers and therapeutic targets, since they are able to regulate specific genes associated with Crohn's disease(CD). In this work, we aim to investigate the composition of gut microbiota of active treatment-na?ve adult CD patients, with miRNA profile from gut microbiota.AIM To investigate the composition of gut microbiota of active treatment-na?ve adult CD patients, with miRNA profile from gut microbiota.METHODS Patients attending the outpatient clinics at Valme University Hospital without relevant co-morbidities were matched according to age and gender. Faecal samples of newonset CD patients, free of treatment, and healthy controls were collected. Faecal samples were homogenized, and DNA was amplified by PCR using primers directed to the 16 S bacterial rRNA gene. Pyrosequencing was performed using GS-Junior platform. For sequence analysis, MGRAST server with the database Ribosomal Project was used. MiRNA profile and their relative abundance were analyzed by quantitative PCR.RESULTS Microbial community was characterized using 16 S rRNA gene sequencing in 29 samples(n = 13 CD patients, and n = 16 healthy controls). The mean Shannon diversity was higher in the healthy control population compared to CD group(5.5 vs 3.7). A reduction in Firmicutes and an increase in Bacteroidetes were found. Clostridia class was also significantly reduced in CD. Principal components analysis showed a grouping pattern, identified in most of the subjects in both groups, showing a marked difference between control and CD groups. A functional metabolic study showed that a lower metabolism of carbohydrates(P = 0.000) was found in CD group, while the metabolism of lipids was increased. In CD patients, three miRNAs were induced in affected mucosa: mir-144(6.2 ± 1.3 fold), mir-519(21.8 ± 3.1) and mir-211(2.3 ± 0.4). CONCLUSION Changes in microbial function in active non-treated CD subjects and three miRNAs in affected vs non-affected mucosa have been found. miRNAs profile may serve as a biomarker. | María Rojas-Feria Teresa Romero-García Jose Angel Fernández Caballero-Rico Helena Pastor Ramírez Marta Avilés-Recio Manuel Castro-Fernandez Natalia Chueca Porcuna Manuel Romero-Gomez Federico García Lourdes Grande JoséA Del Campo | 2018 | World Journal of Gastroenterology2018，24，46： | 7 |
| 2 | Mesenchymal stem cell therapy in retinal and optic nerve diseases: An update of clinical trials显示文摘Retinal and optic nerve diseases are degenerative ocular pathologies which lead to irreversible visual loss. Since the advanced therapies availability, cell-based therapies offer a new all-encompassing approach. Advances in the knowledge of neuroprotection, immunomodulation and regenerative properties of mesenchymal stem cells(MSCs) have been obtained by several preclinical studies of various neurodegenerative diseases. It has provided the opportunity to perform the translation of this knowledge to prospective treatment approaches for clinical practice. Since 2008, several first steps projecting new treatment approaches, have been taken regarding the use of cell therapy in patients with neurodegenerative pathologies of optic nerve and retina. Most of the clinical trials using MSCs are in Ⅰ/Ⅱ phase, recruiting patients or ongoing, and they have as main objective the safety assessment of MSCs using various routes of administration. However, it is important to recognize that, there is still a long way to go to reach clinical trials phase Ⅲ-Ⅳ. Hence, it is necessary to continue preclinical and clinical studies to improve this new therapeutic tool. This paper reviews the latest progress of MSCs in human clinical trials for retinal and optic nerve diseases. | Sonia Labrador-Velandia María Luz Alonso-Alonso Sara Alvarez-Sanchez Jorge González-Zamora Irene Carretero-Barrio José Carlos Pastor Iván Fernandez-Bueno Girish Kumar Srivastava | 2016 | World Journal of Stem Cells2016，8，11： | 5 |
| 3 | Role of serum cytokine profile in ulcerative colitis assessment显示文摘 | Manuel Luis Rodríguez‐Perlvárez Valle García‐Sánchez Carlos Manuel Villar‐Pastor Raál González Eva Iglesias‐Flores Jordi Muntane Federico Gómez‐Camacho | 2012 | Inflamm Bowel Dis2012，，10： | 2 |
| 4 | Above-ground production and N and P cyeling along a nitrogen mineralization gradient on blackhawk island Wisconsin 显示文摘 | PASTOR J ABER J D MCCLAUGHERTY C A | 1984 | Ecology1984，65，： | 1 |
| 5 | Radial DEA models without inputs or without outputs显示文摘 | Pastor J T | 1999 | European Journal of Operational Research1999，118，1： | 1 |
| 6 | Angiotensin Ⅱ stimulates intercellularadhesion molecule-1 (ICAM-1) expression by human vascular endothelial cells and increasessoluble ICAM-1 release in vivo显示文摘 | Pastore L Tessitore A Martinotti S | 1999 | Circulation1999，100，： | 1 |
| 7 | Nitrogen mineralization dynamics in grass monocultures 显示文摘 | Wedin D A Pastor J | 1993 | Oecogogia1993，96，： | 1 |
| 8 | New strategy for the generation of specific D-peptide amyloid inhibitors 显示文摘 | Esteras-Chopo A Pastor MT Serrano L | 2008 | J Mol Biol2008，377，5： | 1 |
| 9 | Enzyme therapy in type I Gaucher disease : comparative efficacy of mannose-terminated , glucocerebrosidase from natural and recombinant sources 显示文摘 | GRABOWSKI G A BARTON N W PASTORES G | 1995 | Ann Intern Med1995，122，1： | 1 |
| 10 | Appendicitis in pregnancy显示文摘 | Patricia A Pastore Dianne M | 2006 | JABFM2006，19，6： | 1 |
| 11 | European Banking Performance Beyond Country Borders: What Really Matters? 显示文摘 | Lozano-Vivas A Pastor J T Hasan I | 2001 | The European Financial Review2001，5，： | 1 |
| 12 | Versatile chelating behavior of benzil bis (thiosemicarbazone) in zinc, cadmium, and nickel complexes显示文摘 | Lopez-Torres E Mendiola M A Pastor C J | 2004 | Inorg Chem2004，43，17： | 1 |
| 13 | Model - Driven Devel- opment: piecing together the MDA jigsaw 显示文摘 | Pastor O Espa a S Aquino J | 2008 | Informa- tik - Spektrum Special issue on Modelling2008，31，5： | 1 |
| 14 | The behaviour of 5- hydroxymethylcytosine in bisulfite sequencing显示文摘 | Huang Y Pastor W A Shen Y | 2010 | PLoS One2010，5，1： | 1 |
| 15 | Mechanization process in the production of mescal 显示文摘 | Hector M Duran-Garcia Emilio J Gonzalez- Galv a n Pastor Matadamas-Ort i z | 2007 | Journal of Food Agriculture & Environment2007，5，34： | 1 |
| 16 | Analysis of glutathione:implication in redox and detoxification显示文摘 | Pastore A Federici G Bertini E | 2003 | Clin Chim Acta2003，333，1： | 1 |
| 17 | An Efficiency Comparison of European Banking Systems Operating under Different Environmental Conditions 显示文摘 | Lozano-Vivas A Pastor J T Pastor J M | 2002 | Journal of Productivity Analysis2002，18，： | 1 |
| 18 | Dynamical and correlation properties of the Internet 显示文摘 | PASTOR R SATOORAS A VAZQUEZ A | 2001 | Physical Review Letters2001，87，25： | 1 |
| 19 | Asymptomatic Brugada syndrome case unmasked during dimenhydrinate infusion显示文摘 | Pastor A Nunez A Cantale C Cos?o FG | 2001 | J Card-iovasc Electrophysiol2001，，10： | 1 |
| 20 | A review of analytical applications of calorimetry显示文摘 | Ferguson H F Frurip D J Pastor A J | 2000 | Thermochimica Acta2000，363，： | 1 |
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