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| 1 | Effect of early preoperative 5-fluorouracil on the integrity of colonic anastomoses in rats显示文摘AIM:To determine the effect of chemotherapy on wound healing by giving early preoperative 5-fluorouracil (5-FU)to rats with colonic anastomoses. METHODS:Sixty Albino-Wistar male rats(median weight,235 g)were used in this study.The rats were fed with standard laboratory food and given tap water ad libitum.The animals were divided into three groups: Group 1:Control group(chemotherapy was not administered),Group 2:Intraperitoneally(IP)administered 5-FU group(chemotherapy was administered IP to animals at a dose of 20 mg/kg daily during the 5 d preceeding surgery),Group 3:Intravenously(IV)administered 5-FU group.Chemotherapy was administered via the penil vein,using the same dosing scheme and duration as the second group.After a 3-d rest to minimize the side effects of chemotherapy,both groups underwent surgery.One centimeter of colon was resected 2 cm proximally from the peritoneal reflection,thensutured intermittently and subsequently end-to-end anastomosed.In each group,half the animals were given anaesthesia on the 3rd postoperative(PO)day and the other half on the 7th PO day,for in vivo analytic procedures.The abdominal incisions in the rats were dissected,all the new and old anastomotic segments were clearly seen and bursting pressures of each anastomotic segment,tissue hydroxyproline levels and DNA content were determined to assess the histologic tissue repair process. RESULTS:When the IV group was compared with the IP group,bursting pressures of the anastomotic segments on the 3rd and 7th PO days,were found to be significantly decreased,hydroxyproline levels at the anastomotic segment on the 7th PO day were significantly decreased(P<0.01). CONCLUSION:In this study,we conclude that early preoperative 5-FU,administered IV,negatively affects wound healing.However,IP administered 5-FU does not negatively affect wound healing. | Leyla Ozel M Sefa Ozel Ahmet Burak Toros Melih Kara Kemal Sirri Ozkan Gurkan Tellioglu Osman Krand Meral Koyuturk Ibrahim Berber | 2009 | World Journal of Gastroenterology2009，15，33： | 7 |
| 2 | Oxidative stress in portal hypertension-induced rats with particular emphasis on nitric oxide and trace metals显示文摘AIM: To investigate the oxidative-stress-related changes in rats with portal hypertension with particular emphasis on nitric oxide (NO) and trace metals. METHODS: Cirrhosis was induced by partial portal vein ligation (PVL) in Wistar rats. The lipid peroxidation marker (malondialdehyde, MDA), antioxidant defense enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and agents known to have antioxidant features including nitric oxide (NO), zinc (Zn), copper (Cu) were determined both in serum and in liver tissue at 4 wk after surgery in PVL and sham-operated rats. Portal pressure of all experimental animals was measured. MDA was detected by thiobarbituric acid reactivity assay.SOD activity was determined by inhibition of nitroblue tetrazolium reduction with xanthine/xanthine oxidase used asa superoxide generator. CAT activity was determined by the breakdown of hydrogen peroxide. GSH concentrations were measured by using metaphosphoric acid for protein precipitation and 5'-5'-dithio-bis-2-nitrobenzoic acid for color development. NO was detected by the Griess method after reduction of nitrate to nitrite with nitrate reductase, and the concentrations of Zn and Cu were measured by a Shimadzu 680 AA atomic absorption spectrometer. Histopathological confirmation was done under light microscope. Statistical analyses were done by Student's t-test, and significance of the difference was tested by the unpaired Mann-Whitney test. P<0.05 was considered statistically significant.RESULTS: Histopathological studies confirmed PVL induced cirrhotic changes. There was a statistically significant difference in portal pressure between PVL and control groups (P<0.001). The results showed significant increases in the levels of MDA and NO in both tissue and serum (P<0.05 and P<0.001, respectively in tissue; P<0.001 for each in serum), and Zn only in tissue (P<0.001)in rats with PVL compared with sham-operated rats. Besides, PVL rats exhibited reduced plasma and tissue GSH, CAT, SOD (P<0.001 for each). Serum and tissue Cu concentration did not change.CONCLUSION: Our findings suggest that PVL in rats induces important biochemical and molecular changes related to oxidative stress in the liver. | Titiz Izzet Krand Osman Unal Ethem Yavuz Nihat Kusaslan Ramazan Dogan Mustafa Uzun Hafize Kiziler Ali Riza Aydemir Birsen Genc Habibe Aydin Seval Simsek Gonul | 2005 | World Journal of Gastroenterology2005，11，23： | 1 |
| 3 | Elective and Emergency Surgery in Chronic Hemodial- ysis Patients显示文摘 | Ozel Leyla Krand Osman Ozel Mustafa Sefa | 2011 | Renal Failure2011，33，： | 1 |
| 4 | Use ofsmooth muscle plasty after intersphincteric rectal resection toreplace a partially resected internal anal sphincter: Long-termfollow-up显示文摘 | Osman Krand Tunc Yalti Gurkan Tellioglu | 2009 | Dis Colon Rectum2009，52，11： | 1 |
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