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| 1 | Proteomics analysis of Trichoplusia ni midgut epithelial cell brush border membrane vesicles显示文摘The insect midgut epithelium is composed of columnar, goblet, and regenerative cells. Columnar epithelial cells are the most abundant and have membrane protrusions that form the brush border membrane (BBM) on their apical side. These increase surface area available for the transport of nutrients, but also provide opportunities for interaction with xenobiotics such as pathogens, toxins and host plant allelochemicals. Recent improvements in proteomic and bioinfbrmatics tools provided an opportunity to determine the proteome of the T. ni BBM in unprecedented detail. This study reports the identification of proteins from BBM vesicles (BBMVs) using single dimension polyacrylamide gel elec? trophoresis coupled with multi-dimensional protein identification technology. More than 3000 proteins were associated with the BBMV of which 697 were predicted to possess either a signal peptide, at least one transmembrane domain or a GPI-anchor signal. Of these, bioinfbrmatics analysis and manual curation predicted that 185 may be associated with the BBMV or epithelial cell plasma membrane. These are discussed with respect to their predicted functions, namely digestion, nutrient uptake, cell signaling, development, cell-cell interactions, and other functions. We believe this to be the most detailed proteomic analysis of the lepidopteran midgut epithelium membrane to date, which will provide information to better understand the biochemical, physiological and pathological processes taking place in the larval midgut. | Muhammad Afzal Javed Cathy Coutu David A. Theilmann Martin A. Erlandson Dwayne D. Hegedus | 2019 | Insect Science2019，26，3： | 1 |
| 2 | Phage receptor binding protein-based magnetic enrichment method as an aid for real time PCR detection of foodborne bacteria显示文摘 | Somayyeh Poshtiban Muhammad Afzal Javed Denis Arutyunov | 2013 | Analyst2013，138，19： | 1 |
| 3 | Thoracic impalement injury: A survivor with large metallic object in-situ显示文摘Impalement injuries, is a severe form of trauma, which are not common in civilian life. These injuries rarely occurs in major accidents. Abdomen, chest, limbs and perineum are often involved due to their large surface area. Thoracic impalement injury is usually a fatal injury, due to location of major vessels and heart in the thoracic cavity. These injuries are horrifying to site, but the patients who are lucky enough to make it to hospital, usually survive. Chances of survival are larger in right sided impalement injuries while central injuries are always died at the scene.Our patient, 25 years old male, was brought to the emergency room (ER) with large impaled metallic bar (about 2.5 feet long) in situ, in right sided chest. The patient was immediately shifted to operation room (OR) and was operated, his recovery was uneventful without any sequelae.Such patients should be treated and resuscitated according to advanced trauma life support (ATLS) protocols and operated without any delay for further investigations. Such operations are carried out by the most experienced surgeon team available. The impaled objects should not be processed if not necessary to avoid major hemorrhage and damage to vital structures, until the patient is in operation room. Large size and unusual position of impaled objects, makes the job difficult for surgeons/anesthetists.Although horrifying at scene, patients with thoracic impalement injuries are mostly young and healthy, and those who survive the pre-hospital phase are potentially manageable with proper resuscitation. Usually these patients make recovery without any further complications. | Randhawa Muhammad Afzal Muhammad Armughan Muhammad Waqas Javed Usman Ali Rizvi Sajida Naseem | 2018 | Chinese Journal of Traumatology2018，21，6： | 1 |
| 4 | Binary Oriented Feature Selection for Valid Product Derivation in Software Product Line显示文摘Software Product Line(SPL)is a group of software-intensive systems that share common and variable resources for developing a particular system.The feature model is a tree-type structure used to manage SPL’s common and variable features with their different relations and problem of Crosstree Constraints(CTC).CTC problems exist in groups of common and variable features among the sub-tree of feature models more diverse in Internet of Things(IoT)devices because different Internet devices and protocols are communicated.Therefore,managing the CTC problem to achieve valid product configuration in IoT-based SPL is more complex,time-consuming,and hard.However,the CTC problem needs to be considered in previously proposed approaches such as Commonality VariabilityModeling of Features(COVAMOF)andGenarch+tool;therefore,invalid products are generated.This research has proposed a novel approach Binary Oriented Feature Selection Crosstree Constraints(BOFS-CTC),to find all possible valid products by selecting the features according to cardinality constraints and cross-tree constraint problems in the featuremodel of SPL.BOFS-CTC removes the invalid products at the early stage of feature selection for the product configuration.Furthermore,this research developed the BOFS-CTC algorithm and applied it to,IoT-based feature models.The findings of this research are that no relationship constraints and CTC violations occur and drive the valid feature product configurations for the application development by removing the invalid product configurations.The accuracy of BOFS-CTC is measured by the integration sampling technique,where different valid product configurations are compared with the product configurations derived by BOFS-CTC and found 100%correct.Using BOFS-CTC eliminates the testing cost and development effort of invalid SPL products. | Muhammad Fezan Afzal Imran Khan Javed Rashid Mubbashar Saddique Heba G.Mohamed | 2023 | Computers, Materials & Continua2023，76，9： | 0 |
| 5 | Investigation of ageing effects on the electrical properties of polyaniline/silver nanocomposites显示文摘Polyaniline(PANI)/Ag nanocomposites,synthesized by incorporation of separately prepared silver nanoparticles in 1-methyl-2-pyrrolidinone(NMP) solution of PANI,have been aged at the accelerated temperature of 120℃ to simulate a storage period of 2 years at 25℃.The accelerated ageing of these materials is done by using the activation energy calculated from data collected using heat flow calorimetry(HFC).The impedance spectroscopic studies of NMP plasticized aged nanocomposite films suggest a microphase separation into reduced and oxidized repeat units.There is crosslinking of the PANI films during ageing thereby obstructing the charge transfer between PANI chains and silver nanoparticles.As a result,the resistivity is increased. | Asma Binat Afzal Muhammad Javed Akhtar | 2011 | Chinese Physics B2011，20，5： | 0 |
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