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| 1 | 非清髓性骨髓移植诱导异基因受者小鼠免疫耐受的实验研究显示文摘本研究通过非清髓性预处理方案联合髓腔内骨髓移植(IBMBMT)建立异基因小鼠免疫耐受模型,并探讨其诱导耐受的机理。受鼠为雌性C57BL/6(H2b,B6)小鼠,于第0天接受60Coγ线全身照射(TBI),4小时内输注雄性BALB/c(H2d)小鼠来源的骨髓细胞(BMC),2天后腹腔注射环磷酰胺(CTX)。通过皮肤移植、混合淋巴细胞反应(MLR)检测耐受状态,并通过体外过继转移实验、IL2逆转实验等探讨免疫耐受的机制。结果显示,经骨髓移植的B6小鼠对BALB/c小鼠的皮肤移植物平均存活时间(MST)>150天,较对照组明显延长(P<0.01);骨髓移植后第90天,受鼠(黑色)表型开始呈现供鼠(白色)颜色特征。MLR结果证明,B6小鼠获得供体特异性耐受,该耐受可以被IL2逆转且可被过继转移;所有受鼠均未出现GVHD表现。结论:非清髓预处理联合髓腔内骨髓移植可以有效地诱导异基因小鼠免疫耐受,克隆无能、抑制细胞存在及嵌合体产生均参与耐受的形成。 | 陈宝安 毕延智 丁家华 张琰 高冲 孙耘玉 赵钢 王骏 程坚 孙雪梅 Michael Schmitt Hartmut Dhner | 2005 | 中国实验血液学杂志2005，13，6： | 7 |
| 2 | 尼洛替尼联合5-溴汉防己甲素逆转K562/A02细胞耐药的研究显示文摘目的 探讨尼洛替尼、5-溴汉防己甲素(BrTet)及其联合应用对K562/A02细胞多药耐药的逆转作用及其机制.方法 尼洛替尼、BrTet单独或联合作用于K562/A02细胞,应用MTY法检测细胞增殖抑制率、流式细胞术检测细胞凋亡率、RT-PCR检测mdr1 mRNA的表达及Western blot分析P糖蛋白(P-gp)的表达的情况.结果 5 nmol/L尼洛替尼和0.5μmol/L BrTet单独使用48 h后,柔红霉素(DNR)对K562/A02细胞的IC50分别为4.52 mg/L和5.41 mg/L,联合使用后,DNR对K562/A02细胞的IC50降为2.98 mg/L.单用DNR、尼洛替尼和BrTet均不能增加K562/A02细胞凋亡率(P〉0.05),DNR联合尼洛替尼和BrTet后细胞凋亡率明显增高.单用5 nmol/L尼洛替尼和0.5μmol/L BrTet 48 h后,K562/A02细胞mdr1 mRNA灰度值为0.48±0.04、0.64±0.01,两者合用K562/A02细胞mdr1 mRNA灰度值下降为0.35±0.04.单用5 nmol/L尼洛替尼作用48 h,P-gp的表达水平为0.61±0.05;单用0.5μmoL/L BrTet作用48 h,P-gp的表达水平为0.52 ±0.02;两者合用K562/A02细胞P-gp的表达水平降为0.44±0.03.结论 单独应用尼洛替尼和BrTet均可部分逆转K562/A02细胞的耐药,机制可能与降低mdr1 mRNA和P-gp的表达及增加K562/A02细胞凋亡有关,并且两药联用具有明显的协同作用. | 陈宝安 单学赟 程坚 王飞 丁家华 高冲 赵刚 王雪梅 许文林 高峰 夏国华 Michael Schmitt | 2010 | 中华血液学杂志2010，31，6： | 4 |
| 3 | 联合应用尼洛替尼和汉防己甲素逆转K562/A02细胞耐药与诱导凋亡的研究显示文摘本研究旨在探讨联合应用尼洛替尼(nilotinib)和汉防己甲素(tetrandrine,Tet)逆转K562/A02细胞耐药与诱导凋亡的相关性及其机制。应用MTT法检测细胞敏感性,计算尼洛替尼和Tet干预后的柔红霉素(DNR)的半数抑制浓度(IC50),用流式细胞术(FCM)检测细胞凋亡率,RT-PCR检测bax、survivin mRNA的表达及Western blot分析BAX、SURVIVIN蛋白的表达情况。结果表明:5 nmol/L尼洛替尼或1.0μml/L Tet单独作用48小时后,DNR对K562/A02细胞的IC50分别为(5.71±0.72)mg/L和(6.52±0.43)mg/L,两药联合应用时DNR对K562/A02细胞的IC50降为(3.12±0.13)mg/L。单用尼洛替尼或Tet均能增加经DNR作用的K562/A02细胞的凋亡率,两药联合作用时凋亡率增高更明显。单用5 nmol/L尼洛替尼或1.0μml/L Tet 48小时后可上调bax mRNA及其蛋白的表达,且两药联合作用时上调幅度明显。单用5 nmol/L尼洛替尼或1.0μml/LTet48小时后可下调survivin mRNA及其蛋白表达水平,且两药联合作用时下调幅度明显。结论:K562/A02细胞对DNR耐药,单独应用尼洛替尼和Tet均可部分逆转K562/A02细胞对DNR的耐药,并可增加K562/A02细胞的凋亡率,两药联用具有明显的协同作用,其机制可能与上调bax mRNA及其蛋白的表达和下调survivin mRNA及其蛋白的表达有关。 | 崔婷允 陈宝安 丁家华 高冲 程坚 鲍文 仲悦娇 单学赟 高峰 夏国华 Anita Schmitt Michael Schmitt | 2011 | 中国实验血液学杂志2011，19，1： | 4 |
| 4 | 分散剂在乳胶漆中的应用显示文摘综合实验结果和实际使用情况,讨论了涂料生产中聚磷酸盐与聚丙烯酸盐分散剂的应用情况,包括二者的配用以及在不同涂料体系中分散剂的选择. | 范晓玲 Michael Schmitt T.Staffel | 2002 | 涂料工业2002，32，3： | 3 |
| 5 | Biological activity of probable/possible high‐risk human papillomavirus types in cervical cancer显示文摘 | Gordana Halec Markus Schmitt Bolormaa Dondog Enkhtuya Sharkhuu Nicolas Wentzensen Tarik Gheit Massimo Tommasino Friedrich Kommoss Franz X. Bosch Silvia Franceschi Gary Clifford Lutz Gissmann Michael Pawlita | 2012 | Int J Cancer2012，，1： | 1 |
| 6 | Frameshift-derived neoantigens constitute immunotherapeutic targets for patients with microsatellite-instable haematological malignancies显示文摘 | Claudia Maletzki Fabian Schmidt Wilhelm G. Dirks Michael Schmitt Michael Linnebacher | 2013 | European Journal of Cancer2013，，11： | 1 |
| 7 | Alfalfa autotoxicity: Effects of reseeding delay, original stand age, and cultivar显示文摘 | Philippe Seguin Craig C Sheaffer Michael A Schmitt | 2002 | Agron J2002，94，： | 1 |
| 8 | In- season fertilizer nitrogen applications for soybean in minnesota 显示文摘 | Michael A Schmitt John A Lamb Gyles W Randall | 2001 | Agron J2001，93，： | 1 |
| 9 | Flexi- ble technologies and Smart Clothing for Citizen Medicine, Home Healthcare, and Disease Prevention 显示文摘 | Fabrice Axisa Pierre Michael Schmitt Claudine Gehin | 2005 | Ieeetransactions on Informa- tion Technology in Biomedincinc2005，9，3： | 1 |
| 10 | Flexible technologies and smart clothing for citizen medicine, home healthcare, and disease prevention 显示文摘 | AXISA Fabrice SCHMITT Pierre Michael GEHIN Claudine | 2005 | IEEE Transactions on Information Technology in Biomedincine2005，9，3： | 1 |
| 11 | Validity and Reliability of the Alzheimer?s Disease Cooperative Study-Clinical Global Impression of Change显示文摘 | Lon S. Schneider Jason T. Olin Rachelle S. Doody Christopher M. Clark John C. Morris Barry Reisberg Frederick A. Schmitt Michael Grundman Ronald G. Thomas Steven H. Ferris | 1997 | Alzheimer Disease & Associated Disorders1997，，： | 1 |
| 12 | Involvement of Hypoxia-Inducible Transcription Factors in Polycystic Kidney Disease显示文摘 | Wanja Michael Bernhardt Michael Sean Wiesener Alexander Weidemann Roland Schmitt Wilko Weichert Philipp Lechler Valentina Campean Albert Chee Meng Ong Carsten Willam Norbert Gretz Kai-Uwe Eckardt | 2007 | The American Journal of Pathology2007，，3： | 1 |
| 13 | Flexible Technologies and Smart Clothing fi~r Citizen Medicine, Home Healthcare, and Disease Prevention显示文摘 | Fabric Axisa Pierre Michael Schmitt | 2005 | IEEE TRANSACTIONS ON INFORMATION TECHNOLOGY IN BIOMEDICINE2005，9，3： | 1 |
| 14 | Clones or no clones:genetic structure of riparian Populus euphratica forests in Central Asia显示文摘Many riparian(Tugai) forests growing along rivers in arid and hyper-arid regions of Central Asia are dominated by the Euphrates poplar(Populus euphratica). Besides generative reproduction, which is only possible upon flooding events and at a distance to the groundwater of less than 2 m, this phreatophytic tree species also reproduces vegetatively by forming clones that can cover land surface areas of several hectares. Along a gradient of groundwater distances, we investigated whether the fraction of clones in P. euphratica stands(1) increases with increasing distance to the water table;(2) is higher if supplied with water via river cut-offs; and(3) approaches 100% at a short distance to the groundwater, but at high salt concentrations in the upper soil layers, which would prevent germination and establishment of seedlings. AFLP(Amplified Fragment Length Polymorphism) analyses were conducted on leaf samples taken from mature P. euphratica trees growing at the fringes of the Taklimakan Desert in stands with different distances(2–12 m) to the groundwater at two plots at the middle and the lower reaches of the Tarim River and in a stand close to Ebinur Lake, Xinjiang, China. Genetic diversity was large among plots, but considerably smaller within plots. We found the highest genetic diversity(caused by regeneration from seeds) at plots that have a short distance to the groundwater or are supplied with additional water. There was no significant relationship between groundwater distance and clonal fraction. All investigated trees at the saline Ebinur Lake site belonged to one single clone. Our results demonstrate that the genetic pattern of this widespread species is not easily predictable even over small distances as it is a result of a complex interplay of stand history and dispersal of propagules(pollen, seeds, and vegetative diaspores) by wind and water. In conservation and restoration schemes, P. euphratica stands with a high genetic diversity and stands that grow at short distances to the water table and are regularly subjected to flooding(which favors generative over clonal reproduction) should be prioritized. | Katja KRAMP Thomas SCHMITT Petra LANG Michael JESCHKE Philipp SCHAFERI Dustin KULANEK ZHANG Ximing YU Ruide Frank M THOMAS | 2018 | Journal of Arid Land2018，10，5： | 1 |
| 15 | Modulation of lymphocyte subpopulations by extracorporeal photopheresis in patients with acute graft-versus-host disease or graft rejection显示文摘 | Katrin Lorenz Katharina Rommel Jiju Mani Nan Jin Inken Hilgendorf Anthony D. Ho Mathias Freund Michael Schmitt Anita Schmitt | 2015 | Leukemia & Lymphoma2015，，3： | 1 |
| 16 | Detection of Neuroendocrine Tumors of the Small Bowel by Double Balloon Enteroscopy显示文摘 | Michael Bellutti Lucia C. Fry Johannes Schmitt Marcus Seemann Silke Klose Peter Malfertheiner Klaus M?nkemüller | 2009 | Digestive Diseases and Sciences2009，，5： | 1 |
| 17 | Photo- curing of off-set printing inks by functionalized ZnO nano- particles显示文摘 | Michael Schmitt Sylvia Kuhn Mathias Wotocek | 2011 | Journal of Research in Physical Chemistry & Chemical Physics2011，225，3： | 1 |
| 18 | Dream recall frequency and nightmare frequency in patients with sleep-disordered breathing 显示文摘 | Michael Schredl Judith Schmitt | 2009 | Somnologie2009，13，1： | 1 |
| 19 | Detection of lung cancer using weighted digital analysis of breath biomarkers显示文摘 | Michael Phillips Nasser Altorki John H.M. Austin Robert B. Cameron Renee N. Cataneo Robert Kloss Roger A. Maxfield Muhammad I. Munawar Harvey I. Pass Asif Rashid William N. Rom Peter Schmitt James Wai | 2008 | Clinica Chimica Acta2008，，2： | 1 |
| 20 | Differential effects of venlafaxine in the treatment of major depressive disorder according to baseline severity显示文摘 | Andreas B. Schmitt Michael Bauer Hans-Peter Volz Hans-Jürgen Moeller Qin Jiang Philip T. Ninan Peter-Andreas Loeschmann | 2009 | European Archives of Psychiatry and Clinical Neuroscience2009，，6： | 1 |
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