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您的检索式：作者名="Michael Pacher"
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| 1 | The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology显示文摘Since the discovery that nucleases of the bacterial CRISPR(clustered regularly interspaced palindromic repeat)-associated(Cas) system can be used as easily programmable tools for genome engineering,their application massively transformed different areas of plant biology. In this review, we assess the current state of their use for crop breeding to incorporate attractive new agronomical traits into specific cultivars of various crop plants. This can be achieved by the use of Cas9/12 nucleases for double-strand break induction,resulting in mutations by non-homologous recombinatr e-tion. Strategies for performing such experiments à from Rthe design of guide RNA to the use of different transformation technologies à are evaluated. Furtherweive-more, we sum up recent developments regarding the use of nuclease-deficient Cas9/12 proteins, as DNAbinding moieties for targeting different kinds of enzyme activities to specific sites within the genome. Progress in base deamination, transcriptional induction and transcriptional repression, as well as in imaging in plants, is also discussed. As different Cas9/12 enzymes are at hand, the simultaneous application of various enzyme activities, to multiple genomic sites, is now in reach to redirect plant metabolism in a multifunctional manner and pave the way for a new level of plant synthetic biology. | Jochen Kumlehn Janine Pietralla Goetz Hensel Michael Pacher Holger Puchta | 2018 | Journal of Integrative Plant Biology2018，60，12： | 9 |
| 2 | Impact of constitutive IGF1/IGF2 stimulation on the transcriptional program of human breast cancer cells显示文摘 | Pacher M Michael J Mario S | | 0，，01： | 1 |
| 3 | Measuring extracellular matrix turnover in the serum of patients with idiopathic or ischemic dilated cardiomyopathy and impact on diagnosis and prognosis显示文摘 | Gu¨nter Klappacher Patrick Franzen Doris Haab Mohammad Mehrabi Michael Binder Karina Plesch Richard Pacher Michael Grimm Ingrid Pribill Hans-Georg Eichler H. Dietmar Glogar | 1995 | The American Journal of Cardiology1995，，14： | 1 |
| 4 | Diabetische Herzinsuffizienz显示文摘 | Michael Resl Martin Hülsmann Richard Pacher Martin Clodi | 2009 | Wiener Medizinische Wochenschrift2009，，5： | 1 |
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