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| 1 | Plant Prime Editors Enable Precise Gene Editing in Rice Cells显示文摘Genome editing is revolutionizing plant research and crop breeding.Sequence-specific nucleases(SSNs)such as zinc finger nuclease(ZFN)and TAL effector nuclease(TALEN)have been used to create site-specific DNA double-strand breaks and to achieve precise DNA modifications by promoting homology-directed repair(HDR)(Steinert et al.,2016;Voytas,2013).Later,RNA-guided SSNs such as CRISPR-Cas9,Cas12a,Cas12b,and their variants were applied for genome editing in plants(Li et al.,2013;Nekrasov et alM 2013;Tang et al.,2017;Zhong et al.,2019;Ming et al.,2020;Tang et al.,2019).However,HDR relies on simultaneous delivery of SSNs and DNA donors,which has been challenging in plants(Steinert et al.,2016;Zhang et aL,2019).Another challenge for realizing efficient HDR in plants is that DNA repair favors nonhomologous end joining(NHEJ)pathways over HDR in most cell types(Puchta,2005;Qi et al.,2013). | Xu Tang Simon Sretenovic Qiurong Ren Xinyu Jia Mengke Li Tingting Fan Desuo Yin Shuyue Xiang Yachong Guo Li Liu Xuelian Zheng Yiping Qi Yong Zhang | 2020 | Molecular Plant2020，13，5： | 20 |
| 2 | An improved ground motion intensity measure for super high-rise buildings显示文摘Ground motion intensity measure (IM) is an important part in performance-based seismic design. A reasonable and efficient IM can make the prediction of the structural seismic responses more accurate. Therefore, a more reasonable IM for super high-rise buildings is proposed in this paper. This IM takes into account the significant characteristic that higher-order vibration modes play important roles in the seismic response of super high-rise buildings, as well as the advantages of some existing IMs. The key parameter of the proposed IM is calibrated using a series of time-history analyses. The collapse simulations of two super high-rise buildings are used to discuss the suitability of the proposed IM and some other existing IMs. The results indicate that the proposed IM yields a smaller coefficient of variation for the critical collapse status than other existing IMs and performs well in reflecting the contribution of higher-order vibration modes to the structural response. Hence, the proposed IM is more applicable to seismic design for super high-rise buildings than other IMs. | LU Xiao YE LiePing LU XinZheng LI MengKe MA XiaoWei | 2013 | Science China(Technological Sciences)2013，56，6： | 9 |
| 3 | Molecular crowding agents engineered to make bioinspired electrolytes for high-voltage aqueous supercapacitors显示文摘The development of low-cost and eco-friendly aqueous electrolytes with a wide voltage window is the key to achieving safe high energy density supercapacitors(SCs).In this work,a molecular crowding electrolyte is prepared by simulating the crowded environment in living cells.Ion transport in the molecular crowding electrolyte can be effectively improved via reducing the molecular weight of the crowding agent,polyethylene glycol(PEG).The results show that PEG with a molecular weight of 200(PEG200)can significantly improve ionic conductivity while maintaining a wide voltage window.These advantages enable commercial activated carbon-based SCs to work at 2.5 V with high energy density,outstanding rate performance and good stability for more than 10,000 cycles.On this basis,three series of molecular crowding electrolytes using sodium perchlorate,lithium perchlorate,and sodium trifluoromethanesulfonate as salts are developed,demonstrating the versatility of PEG200 for wide-voltage aqueous electrolytes. | Mengke Peng Li Wang Longbin Li Zhongyou Peng Xiannong Tang Ting Hu Kai Yuan Yiwang Chen | 2021 | eScience2021，1，1： | 5 |
| 4 | MATRIEX imaging:multiarea two-photon real-time in vivo explorer显示文摘Two-photon laser scanning microscopy has been extensively applied to study in vivo neuronal activity at cellular and subcellular resolutions in mammalian brains.However,the extent of such studies is typically confined to a single functional region of the brain.Here,we demonstrate a novel technique,termed the multiarea two-photon real-time in vivo explorer(MATRIEX),that allows the user to target multiple functional brain regions distributed within a zone of up to 12mm in diameter,each with a field of view(FOV)of ~200μm in diameter,thus performing two-photon Ca2+imaging with single-cell resolution in all of the regions simultaneously.For example,we demonstrate real-time functional imaging of single-neuron activities in the primary visual cortex,primary motor cortex and hippocampal CA1 region of mice in both anesthetized and awake states.A unique advantage of the MATRIEX technique is the configuration of multiple microscopic FOVs that are distributed in three-dimensional space over macroscopic distances(>1 mm)both laterally and axially but that are imaged by a single conventional laser scanning device.In particular,the MATRIEX technique can be effectively implemented as an add-on optical module for an existing conventional single-beam-scanning two-photon microscope without requiring any modification to the microscope itself.Thus,the MATRIEX technique can be readily applied to substantially facilitate the exploration of multiarea neuronal activity in vivo for studies of brain-wide neural circuit function with single-cell resolution. | Mengke Yang Zhenqiao Zhou Jianxiong Zhang Shanshan Jia Tong Li Jiangheng Guan Xiang Liao Bing Leng Jing Lyu Kuan Zhang Min Li Yan Gong Zhiming Zhu Junan Yan Yi Zhou Jian K Liu Zsuzsanna Varga Arthur Konnerth Yuguo Tang Jinsong Gao Xiaowei Chen Hongbo Jia | 2019 | Light(Science & Applications)2019，8，1： | 4 |
| 5 | Influence of soil——structure interaction on seismic collapse resistance of super-tall buildings显示文摘Numerous field tests indicate that the soilestructure interaction(SSI) has a significant impact on the dynamic characteristics of super-tall buildings,which may lead to unexpected structural seismic responses and/or failure.Taking the Shanghai Tower with a total height of 632 m as the research object,the substructure approach is used to simulate the SSI effect on the seismic responses of Shanghai Tower.The refined finite element(FE) model of the superstructure of Shanghai Tower and the simplified analytical model of the foundation and adjacent soil are established.Subsequently,the collapse process of Shanghai Tower taking into account the SSI is predicted,as well as its final collapse mechanism.The influences of the SSI on the collapse resistance capacity and failure sequences are discussed.The results indicate that,when considering the SSI,the fundamental period of Shanghai Tower has been extended significantly,and the collapse margin ratio has been improved,with a corresponding decrease of the seismic demand.In addition,the SSI has some impact on the failure sequences of Shanghai Tower subjected to extreme earthquakes,but a negligible impact on the final failure modes. | Mengke Li Xiao Lu Xinzheng Lu Lieping Ye | 2014 | Journal of Rock Mechanics and Geotechnical Engineering2014，6，5： | 4 |
| 6 | The hypoxia adaptation of small mammals to plateau and underground burrow conditions显示文摘Oxygen is one of the important substances for the survival of most life systems on the earth,and plateau and underground burrow systems are two typical hypoxic environments.Small mammals living in hypoxic environments have evolved different adaptation strategies,which include increased oxygen delivery,metabolic regulation of physiological responses and other physiological responses that change tissue oxygen utilization.Multiomics predictions have also shown that these animals have evolved different adaptations to extreme environments.In particular,vascular endothelial growth factor(VEGF)and erythropoietin(EPO),which have specific functions in the control of O_(2) delivery,have evolved adaptively in small mammals in hypoxic environments.Naked mole-rats and blind mole-rats are typical hypoxic model animals as they have some resistance to cancer.This review primarily summarizes the main living environment of hypoxia tolerant small mammals,as well as the changes of phenotype,physiochemical characteristics and gene expression mode of their long-term living in hypoxia environment. | Mengke Li Dan Pan Hong Sun Lei Zhang Han Cheng Tian Shao Zhenlong Wang | 2021 | Animal Models and Experimental Medicine2021，4，4： | 3 |
| 7 | Frictional stability of Longmaxi shale gouges and its implication for deep seismic potential in the southeastern Sichuan Basin显示文摘Microearthquakes accompanying shale gas recovery highlight the importance of exploring the frictional and stability properties of shale gouges.Aiming to reveal the influencing factors on fault stability,this paper explores the impact of mineral compositions,effective stress and temperature on the frictional stability of Longmaxi shale gouges in deep reservoirs located in the Luzhou area,southeastern Sichuan Basin.Eleven shear experiments were conducted to define the frictional strength and stability of five shale gouges.The specific experimental conditions were as follows:temperatures:90–270°C;a confining stress:95 MPa;and pore fluid pressures:25–55 MPa.The results show that all five shale gouges generally display high frictional strength with friction coefficients ranging from 0.60 to 0.70 at the aforementioned experiment condition of pressures,and temperatures.Frictional stability is significantly affected by temperature and mineral compositions,but is insensitive to variation in pore fluid pressures.Fault instability is enhanced at higher temperatures(especially at>200°C)and with higher tectosilicate/carbonate contents.The results demonstrate that the combined effect of mineral composition and temperature is particularly important for induced seismicity during hydraulic fracturing in deep shale reservoirs. | Fengshou Zhang Li Cui Mengke An Derek Elsworth Changrong He | 2022 | Deep Underground Science and Engineering2022，1，1： | 2 |
| 8 | Synthesis of ordered Si nanowire arrays in porous anodic aluminum oxide templates显示文摘Highly ordered poly crystalline Si nanowire arrays were synthesized in porous anodic aluminum oxide (AAO) templates by the chemical vapor deposition (CVD) method. The morphological structure, the crystal character of Si nanowire arrays and the individual nanowire were analyzed by the transmission electron microscopy (TEM), scanning electron microscopy (SEM), atom force microscopy (AFM) and the X-ray diffraction spectrum (XRD), respectively. It is shown that most fabricated silicon nanowires (SiNWs) tend to be assembled parallelly in bundles and constructed with highly orientated arrays. This method provides a simple and low cost fabricating craftwork and the diameters and lengths of SiNWs can be controlled, the large area Si nanowire arrays can be achieved easily under such a way. The curling and twisting SiNWs are fewer than those by other synthesis methods. | Mengke Li Chengwei Wang Hulin Li | 2001 | Chinese Science Bulletin2001，46，21： | 2 |
| 9 | 显示文摘 | Li Mengke Lu Mei Kong Lingbin | 2003 | Materials Science and Engineer A2003，354，： | 1 |
| 10 | Well-aligned polyaniline nano-fibril array membrane and its field emission property显示文摘 | Chengwei Wang Zhen Wang Mengke Li Hulin Li | 2001 | Chemical Physics Letters2001，，5： | 1 |
| 11 | Synthesis and properties of electroless Ni-P-Nanometer Diamond composite coatings显示文摘 | Xu Hui Yang Zhi Li Mengke | 2005 | Surface & Coatings Technology2005，191，： | 1 |
| 12 | Preparation and properties of Ni/P/single-walled carbonnanotubes composite coatings by means of electroless plating 显示文摘 | Yang Zhi Xu Hui Li Mengke | 2004 | Thin Solid Films2004，466，12： | 1 |
| 13 | The synthesis of MWNTS/SWNTS muhiple phase nanowire arrays in porous anodic aluminum oxide templates 显示文摘 | LI Mengke LU Mei KONG Lingbin | 2003 | Materials Science and Engineer A2003，354，1： | 1 |
| 14 | Rational design of eco-friendly Mn-doped nonstoichiometric CuInSe/ZnSe core/shell quantum dots for boosted photoelectrochemical efficiency显示文摘Colloidal core/shell quantum dots(QDs)with environment-friendly feature and controllable optoelectronic properties are promising building blocks in emerging solar technologies.In this work,we rationally design and tailor the eco-friendly CuInSe(CISe)/ZnSe core/shell QDs by Mn doping and stoichiometric optimization(i.e.,molar ratios of Cu/In).It is demonstrated that Mn doping in In-rich CISe/ZnSe core/shell QDs can effectively engineer the charge kinetics inside the QDs,enabling efficient photogenerated electrons transfer into the shell for retarded charge recombination.As a result,a solar-driven photoelectrochemical(PEC)device fabricated using the optimized Mn-doped In-rich CISe/ZnSe core/shell QDs(Cu/In ratio of 1/2)exhibits improved charge extraction and injection,showing a~3.5-fold higher photocurrent density than that of the pristine CISe/ZnSe core/shell QDs under 1 sun AM 1.5G illumination.The findings indicate that transition metal doping in“green”nonstoichiometric core/shell QDs may offer a new strategy for achieving high-efficiency solar energy conversion applications. | Rui Wang Xin Tong Zhihang Long Ali Imran Channa Hongyang Zhao Xin Li Mengke Cai Yimin You Xuping Sun Zhiming Wang | 2022 | Nano Research2022，15，8： | 1 |
| 15 | Branched silver nanowires obtained in porous anodic aluminum oxide template显示文摘 | Kong Lingbin Lu Mei Li Mengke | 2003 | Journal ofMaterials Science Letters2003，22，9： | 1 |
| 16 | Well-aligned carbon nanotube array membrane and its field emission properties显示文摘 | Chengwei Wang Mengke Li Hulin Li | 2001 | Science in China Series A: Mathematics2001，，2： | 1 |
| 17 | Influence factors of documentation quality of bidding for highway engi- neering and the main problem of 显示文摘 | Li Hua Dong Weizhi Wang Mengke | 2009 | Technology Innovation Herald2009，81，： | 1 |
| 18 | Predicting the lattice thermal conductivity of alloyed compounds from the perspective of configurational entropy显示文摘Accurate evaluation of lattice thermal conductivity is usually a tough task from the theoretical side,especially for alloyed systems with fractional stoichiometry.Using the tetradymite family as a prototypical class of examples,we propose a reliable approach for rapid prediction on the lattice thermal conductivity at arbitrary composition by utilizing the concept of configurational entropy.Instead of performing time-consuming first-principles calculations,the lattice thermal conductivities of any alloyed tetradymites can be readily obtained from a few samples with integer stoichiometry.The strong predictive power is demonstrated by good agreement between our results and those reported experimentally.In principle,such an effective method can be applicable to any other material families,which is very beneficial for high-throughput design of systems with desired thermal conductivity. | Mengke Li Guohua Cao Yufeng Luo Caiyu Sheng Huijun Liu | 2022 | npj Computational Materials2022，，1： | 1 |
| 19 | Diacylglycerol-enriched Oil from Hydrolysis of Soybean Oil with Rhizopus Oryzae Lipase Against High-fat Diet-induced Obesity in Mice显示文摘The present work aimed to investigate the impact of intake of diacylglycerol(DAG) from soybean oil on the reduction of fat accumulation and the long-term effects of dietary intake of DAG and triacylglycerol(TAG) with similar fatty acid compositions on the development of obesity. Kuming mice were used to compare the effects of low-dose TAG(2.5 g/kg BW), low-dose DAG(2.5 g/kg BW), high-dose TAG(10 g/kg BW), and high-dose DAG(10 g/kg BW) on the induced obesity. The results showed that the body weight and serum triglyceride concentration decreased significantly in both DAG-treated groups compared with the TAG-treated groups(P < 0.01, P < 0.05, respectively). However, the plasma glucose concentration was significantly lower in the DAG-treated groups than the TAG-treated groups(P < 0.05); the weight and morphology of the liver and kidney in DAG-treated groups were similar to those in the control group, there were no significant differences within each group. The present results indicated the anti-obesity and lipid-lowering effects of dietary DAG oil in mice and its potential use as a functional food for humans. | ZHANG Mengke SHI Bo LI Chao CHENG Yongqiang | 2018 | Grain & Oil Science and Technology2018，1，1： | 1 |
| 20 | Well-aligned carbon nanotube array membrane and its field emission properties显示文摘 | Chengwei Wang Mengke Li Hulin Li | 2001 | Science in China Series A: Mathematics2001，，2： | 1 |
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