|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Design of 16S rRNA gene primers for 454 pyrosequencing of the human foregut microbiome显示文摘AIM:To design and validate broad-range 16S rRNA primers for use in high throughput sequencing to classify bacteria isolated from the human foregut microbiome.METHODS:A foregut microbiome dataset was constructed using 16S rRNA gene sequences obtained from oral,esophageal,and gastric microbiomes produced by Sanger sequencing in previous studies represented by 219 bacterial species.Candidate primers evaluated were from the European rRNA database.To assess the effect of sequence length on accuracy of classification,16S rRNA genes of various lengths were created by trimming the full length sequences.Sequences spanning various hypervariable regions were selected to simulate the amplicons that would be obtained using possible primer pairs.The sequences were compared with full length 16S rRNA genes for accuracy in taxonomic classification using online software at the Ribosomal Database Project (RDP).The universality of the primer set was evaluated using the RDP 16S rRNA database which is comprised of 433 306 16S rRNA genes,represented by 36 phyla.RESULTS:Truncation to 100 nucleotides(nt)downstream from the position corresponding to base 28 in the Escherichia coli 16S rRNA gene caused misclassification of 87(39.7%)of the 219 sequences,compared with misclassification of only 29(13.2%)sequences with truncation to 350 nt.Among 350-nt sequence reads within various regions of the 16S rRNA gene,the reverse read of an amplicon generated using the 343F/798R primers had the least(8.2%)effect on classification.In comparison,truncation to 900 nt mimicking single pass Sanger reads misclassified 5.0%of the 219 sequences.The 343F/798R amplicon accurately assigned 91.8%of the 219 sequences at the species level.Weighted by abundance of the species in the esophageal dataset,the 343F/798R amplicon yielded similar classification accuracy without a significant loss in species coverage(92%).Modification of the 343F/798R primers to 347F/803R increased their universality among foregut species.Assuming that a typicalpolymerase chain reaction can tolerate 2 mismatches between a primer and a template,the modified 347F and 803R primers should be able to anneal 98%and 99.6%of all 16S rRNA genes in the RDP database.CONCLUSION:347F/803R is the most suitable pair of primers for classification of foregut 16S rRNA genes but also possess universality suitable for analyses of other complex microbiomes. | Carlos W Nossa William E Oberdorf Jφrn A Aas Bruce J Paster Todd Z DeSantis Eoin L Brodie Daniel Malamud Michael A Poles Zhiheng Pei | 2010 | World Journal of Gastroenterology2010，16，33： | 16 |
| 2 | Indifference pricing for CRRA utilities显示文摘 | MALAMUD S TRUBOWITZ E WUTHRICH M | 2013 | Mathematics and Financial Economics2013，7，3： | 1 |
| 3 | Contribution of connexin 26mutations to nonsyndromic deafness in Ashkenazi patients and the variable phenotypic effect of the mutation 167delT显示文摘 | Lerer I Sagi M Malamud E | 2000 | Am J Med Genet2000，95，1： | 1 |
| 4 | Astudy of microstrip array antenna with the feed network显示文摘 | LERINE E MALAMUD G SHTRIKMAN S | 1989 | IEEE Transactions on Antennas and Propagation1989，37，4： | 1 |
| 5 | A study of microstrip array antennas with the feed network显示文摘 | Levine E Malamud G Shtrikman S | 1989 | IEEE Trans on AP1989，37，4： | 1 |
| 6 | A study of mi- crostrip array antenna with the feed network显示文摘 | Lerine E Malamud G Shtrikman S | 1989 | IEEE Transactions on Antennas and Propagation1989，37，4： | 1 |
| 7 | The E148Q mutation in the MEFV gene: is it a disease causing mutation or a sequence variant? 显示文摘 | Lerer I Malamud E | 2000 | Hum Mutat2000，15，： | 1 |
| 8 | A study of microstrip array antennas with the feed network 显示文摘 | LEVIN E MALAMUD G HTRIKMAN S S | 1989 | IEEE Trans Antenna Propagation1989，37，4： | 1 |
| 9 | A study of microstrip array antenna with the feed network显示文摘 | LERINE E MALAMUD G SHTRIKMAN S | 1989 | IEEE Trans Antenna Propagation1989，37，4： | 1 |
| 10 | 高血压的数字化管理改善收缩压变异性显示文摘平均收缩压相比,较高的收缩压变异性已被证明是全因和心血管病死亡率、脑卒中和心脏病的更好的预测因子。研究者评估了数字高血压治疗计划对803例长期高血压患者的收缩压变异性的影响,这些患者在入组前接受初级保健医生至少12个月的治疗(平均为4.7年)。血压读数通过使用数据连接的血压装置直接从家中传输。药物调整和生活方式指导通过一个由药剂师和健康顾问组成的专门团队提供。使用每个个体所有收缩压数值的标准差计算收缩压变异性,并按四分位数分组。 | 刘青(译) 叶鹏(摘、审校) Milani RV Wilt JK Milani AR Bober RM Malamud E Entwisle J Lavie CJ | 2020 | 中华高血压杂志2020，28，2： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员