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| 1 | A genome-wide association study uncovers a critical role of the RsPAP2 gene in red-skinned Raphanus sativus L.显示文摘Radish(Raphanus sativus L.)taproot contains high concentrations of flavonoids,including anthocyanins(ATCs),in redskinned genotypes.However,little information on the genetic regulation of ATC biosynthesis in radish is available.A genome-wide association study of radish red skin color was conducted using whole-genome sequencing data derived from 179 radish genotypes.The R2R3-MYB transcription factor production of anthocyanin pigment 2(PAP2)gene was found in the region associated with a leading SNP located on chromosome 2.The amino acid sequence encoded by the RsPAP2 gene was different from those of the other published RsMYB genes responsible for the red skin color of radish.The overexpression of the RsPAP2 gene resulted in ATC accumulation in Arabidopsis and radish,which was accompanied by the upregulation of several ATC-related structural genes.RsPAP2 was found to bind the RsUFGT and RsTT8 promoters,as shown by a dual-luciferase reporter system and a yeast one-hybrid assay.The promoter activities of the RsANS,RsCHI,RsPAL,and RsUFGT genes could be strongly activated by coinfiltration with RsPAP2 and RsTT8.These findings showed the effectiveness of GWAS in identifying candidate genes in radish and demonstrated that RsPAP2 could(either directly or together with its cofactor RsTT8)regulate the transcript levels of ATC-related genes to promote ATC biosynthesis,facilitating the genetic enhancement of ATC contents and other related traits in radish. | Lianxue Fan YanWang Liang Xu Mingjia Tang Xiaoli Zhang Jiali Ying Cui Li Junhui Dong Liwang Liu | 2020 | Horticulture Research2020，7，1： | 2 |
| 2 | A state-of-the-art review of mechanical characteristics and cracking processes of pre-cracked rocks under quasi-static compression显示文摘The mechanical characteristics and failure behavior of rocks containing flaws or discontinuities have received wide attention in the field of rock mechanics.When external loads are applied to rock materials,stress-induced cracks would initiate and propagate from the flaws,ultimately leading to the irreversible failure of rocks.To investigate the cracking behavior and the effect of flaw geometries on the mechanical properties of rock materials,a series of samples containing one,two and multiple flaws have been widely investigated in the laboratory.In this paper,the experimental results for pre-cracked rocks under quasistatic compression were systematically reviewed.The progressive failure process of intact rocks is briefly described to reveal the background for experiments on samples with flaws.Then,the nondestructive measurement techniques utilized in experiments,such as acoustic emission(AE),X-ray computed tomography(CT),and digital image correlation(DIC),are summarized.The mechanical characteristics of rocks with different flaw geometries and under different loading conditions,including the geometry of pre-existing flaws,flaw filling condition and confining pressure,are discussed.Furthermore,the cracking process is evaluated from the perspective of crack initiation,coalescence,and failure patterns. | Liwang Liu Haibo Li Xiaofeng Li | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，6： | 2 |
| 3 | An overview of different distillationtechnologies for drinking water preparation显示文摘 | Lu Liu He Liwang | 2014 | AdvancedMaterials Research2014，937，7： | 1 |
| 4 | Comparative proteomic analysis provides insight into a complex regulatory network of taproot formation in radish (Raphanus sativus L.)显示文摘The fleshy taproot of radish is an important storage organ determining its yield and quality.Taproot thickening is a complex developmental process in radish.However,the molecular mechanisms governing this process remain unclear at the proteome level.In this study,a comparative proteomic analysis was performed to analyze the proteome changes at three developmental stages of taproot thickening using iTRAQ approach.In total,1862 differentially expressed proteins(DEPs)were identified from 6342 high-confidence proteins,among which 256 up-regulated proteins displayed overlapped accumulation in S1(pre-cortex splitting stage)vs.S2(cortex splitting stage)and S1 vs.S3(expanding stage)pairs,whereas 122 up-regulated proteins displayed overlapped accumulation in S1 vs.S3 and S2 vs.S3 pairs.Gene Ontology(GO)and pathway enrichment analysis showed that these DEPs were mainly involved in several processes such as“starch and sucrose metabolism”,“plant hormone signal transduction”,and“biosynthesis of secondary metabolites”.A high concordance existed between iTRAQ and RT-qPCR at the mRNA expression levels.Furthermore,association analysis showed that 187,181,and 96 DEPs were matched with their corresponding differentially expressed genes(DEGs)in S1 vs.S2,S1 vs.S3,and S2 vs.S3 comparison,respectively.Notably,several functional proteins including cell division cycle 5-like protein(CDC5),expansin B1(EXPB1),and xyloglucan endotransglucosylase/hydrolase protein 24(XTH24)were responsible for cell division and expansion during radish taproot thickening process.These results could facilitate a better understanding of the molecular mechanism underlying taproot thickening,and provide valuable information for the identification of critical genes/proteins responsible for taproot thickening in root vegetable crops. | Yang Xie Liang Xu Yan Wang Lianxue Fan Yinglong Chen Mingjia Tang Xiaobo Luo Liwang Liu | 2018 | Horticulture Research2018，5，1： | 1 |
| 5 | TRAP markers generated with resistant gene analog sequences and their application to genetic diversity analysis of radish germplasm显示文摘 | Dandan Cheng Fenjiao Zhang Liwang Liu Liang Xu Yinglong Chen Xianli Wang Cecilia Limera Rugang Yu Yiqin Gong | 2013 | Scientia Horticulturae2013，，： | 1 |
| 6 | Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum L显示文摘 | Liu Liwang Guo Wangzhen Zhu Xiefei | | 0，，106： | 1 |
| 7 | Multicolor fluorescence in situ hybridization with combinatorial labeling probesenables a detailed karyotype analysis of Larix principis-rupprechtii显示文摘 | Liu B Liwang Q Ruiyang C | 2007 | Biol Res2007，40，1： | 1 |
| 8 | Karyotype analysis and physical mapping of 45S rDNA in eight species of Sophora, Robinia, and Amorpha显示文摘The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species,and two forms of Sophora,two species of Robina,and one species of Amorpha.S.japonica L.,S.japonica L.f.oligophylla Franch.,S.japonica L.f.pendula Loud.,and S.xanthantha C.Y.Ma.are all tetraploids with 2n=28.There were four 45S rDNA sites in pericentromeric regions of two pairs of chromosomes in each of them.S.rubriflora Tsoong.is a triploid with 2n=21,and three sites were located in each satellite of group 5 chromosomes.In R.pseudoacacia L.(2n=2x=22),we examined four intensive signals in te-lomeric regions of two pairs of satellite chromosomes.In R.hispida L.(2n=2x=30),there were four other signals in centromeric regions besides those like in R.pseudoacacia.Amorpha fruticosa L.has most chromosomes(2n=40)among the eight materials,however,there were only six 45S rDNA loci and they laid in centromeric regions,and satellites of three pairs of chromosomes.45S rDNA is a valuable chromosomal landmark in karyotype analysis.The distribution and genomic organization of rDNA in the three genera were also discussed. | LIU Bo CHEN Chengbin LI Xiulan QI Liwang HAN Suying | 2006 | Frontiers in Biology2006，1，3： | 0 |
| 9 | RsERF40 contributes to cold stress tolerance and cell expansion of taproot in radish (Raphanus sativus L.)显示文摘The growth and development of taproots are inhibited by cold stress in radish(Raphanus sativus L.).Ethylene-responsive element binding factors(ERF)are key participators in the cold stress response and growth regulation of plants.However,the function of ERF genes in cold tolerance and root development in radish remains elusive.Here,we showed that the secondary growth of radish taproots was inhibited by cold stress.Comparative transcriptome analysis demonstrated that the RsERF40 gene is an important regulator of the cold stress response and root growth regulation.The cold tolerance of transgenic Arabidopsis plants overexpressing the RsERF40 gene was significantly improved.Overexpressing RsERF40 in the cold-sensitive radish genotype and silencing RsERF40 in the cold-tolerant radish genotype indicated that RsERF40 was beneficial for alleviating oxidative damage under cold stress in radish.Transgenic Arabidopsis seedlings showed an increase in the elongation and radial growth of dark-grown roots.RT-qPCR analysis showed that the expression of the cold-related genes(CORs)RsCOR78 and RsCOR413PM1 and the cell wall strengthening-related genes RsCESA6 and RsEXPB3 was upregulated in transgenic Arabidopsis seedlings.Yeast one-hybrid(Y1H)and dual-luciferase reporter assays(DLA)revealed that RsERF40 directly regulates RsCOR78,RsCOR413PM1,RsCESA6 and RsEXPB3 expression,illustrating that RsERF40 enhances cold tolerance and taproot growth by modulating osmotic adjustment and cell wall mechanical strength in radish.In this study,the RsERF40-regulon was firstly found to be a new cold response pathway independent of the CBF-COR pathway conferring cold stress tolerance with increasing radish taproot growth.These results provided novel insight into the molecular mechanism underlying cold stress response and would facilitate the genetic improvement of cold tolerance in radish and other root vegetable crops. | Cui Li Baozhen Mao Kai Wang Liang Xu Lianxue Fan Yan Wang Ying Li Yinbo Ma Lun Wang Liwang Liu | 2023 | Horticulture Research2023，10，3： | 0 |
| 10 | Decoding of Raman spectroscopy-encoded suspension arrays based on the detail constraint cycle domain adaptive model显示文摘Previous studies have already shown that Raman spectroscopy can be used in the encoding of suspension array technology.However,almost all existing convolutional neural network-based decoding approaches rely on supervision with ground truth,and may not be well generalized to unseen datasets,which were collected under different experimental conditions,applying with the same coded material.In this study,we propose an improved model based on CyCADA,named as Detail constraint Cycle Domain Adaptive Model(DCDA).DCDA implements the clasification of unseen datasets through domain adaptation,adapts representations at the encode level with decoder-share,and enforces coding features while leveraging a feat loss.To improve detailed structural constraints,DCDA takes downsample connection and skips connection.Our model improves the poor generalization of existing models and saves the cost of the labeling process for unseen target datasets.Compared with other models,extensive experiments and ablation studies show the superiority of DCDA in terms of classification stability and generalization.The model proposed by the research achieves a classification with an accuracy of 100%when applied in datasets,in which the spectrum in the source domain is far less than the target domain. | Yu Yao Kaiwen Xue Liwang Liu Shanshan Zhu Chengfeng Yue Yanhong Ji | 2022 | Journal of Innovative Optical Health Sciences2022，15，4： | 0 |
| 11 | Melatonin-induced DNA demethylation of metal transporters and antioxidant genes alleviates lead stress in radish plants显示文摘Melatonin(MT)is a tryptophan-derived natural product that plays a vital role in plant response to abiotic stresses,including heavy metals(HMs).However,it remains elusive how exogenous MT mediates lead(Pb)accumulation and detoxification at the methylation and transcriptional levels in radish.In this study,decreased Pb accumulation and increased antioxidant enzyme activity were detected under MT treatment in radish.Single-base resolution maps of DNA methylation under Pb stress(Pb200)and Pb plus MT treatment(Pb_50MT)were first generated.The genome-wide methylation level was increased under Pb stress,while an overall loss of DNA methylation was observed under MT treatment.The differentially methylated region(DMR)-associated genes between Pb_50MT and Pb200 were uniquely enriched in ion binding terms,including cation binding,iron ion binding,and transition metal ion binding.Hyper-DMRs between Pb200 and Control exhibited a decreasing trend of methylation under Pb_50MT treatment.A few critical upregulated antioxidant genes(e.g.,RsAPX2,RsPOD52 and RsGST)exhibited decreased methylation levels under MT treatment,which enabled the radish plants to scavenge lead-induced reactive oxygen species(ROS)and decrease oxidative stress.Notably,several MT-induced HM transporter genes with low methylation(e.g.,RsABCF5,RsYSL7 and RsHMT)and transcription factors(e.g.,RsWRKY41 and RsMYB2)were involved in reducing Pb accumulation in radish roots.These findings could facilitate comprehensive elucidation of the molecular mechanism underlying MT-mediated Pb accumulation and detoxification in radish and other root vegetable crops. | Mingjia Tang Liang Xu Yan Wang Junhui Dong Xiaoli Zhang Kai Wang Jiali Ying Cui Li Liwang Liu | 2021 | Horticulture Research2021，8，1： | 0 |
| 12 | Effect of intermittent joint distribution on the mechanical and acoustic behavior of rock masses显示文摘The mechanical characteristics and acoustic behavior of rock masses are greatly influenced by stochastic joints.In this study,numerical models of rock masses incorporating intermittent joints with different numbers and dip angles were produced using the finite element method(FEM)with the intrinsic cohesive zone model(ICZM).Then,the uniaxial compressive and wave propagation simulations were performed.The results indicate that the joint number and dip angle can affect the mechanical and acoustic properties of the models.The uniaxial compressive strength(UCS)and wave velocity of rock masses decrease monotonically as the joint number increases.However,the wave velocity grows monotonically as the joint dip angle increases.When the joint dip angle is 45°–60°,the UCS of the rock mass is lower than that of other dip angles.The wave velocity parallel to the joints is greater than that perpendicular to the joints.When the dip angle of joints remains unchanged,the UCS and wave velocity are positively related.When the joint dip angle increases,the variation amplitude of the UCS regarding the wave velocity increases.To reveal the effect of the joint distribution on the velocity,a theoretical model was also proposed.According to the theoretical wave velocity,the change in wave velocity of models with various joint numbers and dip angles was consistent with the simulation results.Furthermore,a theoretical indicator(i.e.fabric tensor)was adopted to analyze the variation of the wave velocity and UCS. | Shuaiyang Fu Haibo Li Liwang Liu Di Wu Ben Wang | 2024 | Journal of Rock Mechanics and Geotechnical Engineering2024，16，4： | 0 |
| 13 | Crystallization and Luminescence Properties of Sm3+-Doped SrO-Al2O3-SiO2 Glass-Ceramics显示文摘The Sm^(3+)-doped SrO-Al_2O_3-SiO_2(SAS) glass-ceramics with excellent luminescence properties were prepared by batch melting and heat treatment. The crystallization behavior and luminescent properties of the glass-ceramics were investigated by DTA, XRD, SEM and luminescence spectroscopy. The results indicate that the crystal phase precipitated in this system is monocelsian(SrAl_2Si_2O_8) and with the increase of nucleation/crystallization temperature, the crystallite increases from 66 % to 79 %. The Sm^(3+)-doped SAS glass-ceramics emit green, orange and red lights centered at 565, 605, 650 and 715 nm under the excitation of 475 nm blue light which can be assigned to the 4 G5/2→6 Hj/2(j=5, 7, 9, 11) transitions ofSm^(3+), respectively. Besides, by increasing the crystallization temperature or the concentration ofSm^(3+), the emission lights of the samples located at 565, 605 and 650 nm are intensified significantly. The present results demonstrate that theSm^(3+)-doped SAS glassceramics are promising luminescence materials for white LED devices by fine controlling and combining of these three green, orange and red lights in appropriate proportion. | 李宏 LIU Liwang TANG Xiaozhe WANG Qian Paul W Wang 王伟 | 2017 | Journal of Wuhan University of Technology(Materials Science)2017，32，5： | 0 |
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