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| 1 | Membrane protein reconstitution for functional and structural studies显示文摘Membrane proteins are involved in various critical biological processes,and studying membrane proteins represents a major challenge in protein biochemistry.As shown by both structural and functional studies,the membrane environment plays an essential role for membrane proteins.In vitro studies are reliant on the successful reconstitution of membrane proteins.This review describes the interaction between detergents and lipids that aids the understanding of the reconstitution processes.Then the techniques of detergent removal and a few useful techniques to refine the formed proteoliposomes are reviewed.Finally the applications of reconstitution techniques to study membrane proteins involved in Ca2+ signaling are summarized. | WANG LiGuo TONGGU LiGe | 2015 | Science China(Life Sciences)2015，58，1： | 5 |
| 2 | Photochemical oxidation of dimethylsulfide in seawater显示文摘Dimethylsulfide(DMS) is generally thought to be lost from the surface oceans by evasion into the atmosphere as well as consumption by microbe.However,photochemical process might be important in the removal of DMS in the oceanic photic zone.A kinetic investigation into the photochemical oxidation of DMS in seawater was performed.The photo-oxidation rates of DMS were influenced by various factors including the medium,dissolved oxygen,photosensitizers,and heavy metal ions.The photo-oxidation rates of DMS were higher in seawater than in distilled water,presumably due to the effect of salinity existing in seawater.Three usual photosensitizers(humic acid,fulvic acid and anthroquinone),especially in the presence of oxygen,were able to enhance the photo-oxidation rate of DMS,with the fastest rate observed with anthroquinone.Photo-oxidation of DMS followed first order reaction kinetics with the rate constant ranging from 2.5×10-5 to 34.3×10-5 s-1.Quantitative analysis showed that approximately 32% of the photochemically removed DMS was converted to dimethylsulfoxide.One of the important findings was that the presence of Hg2+ could markedly accelerate the photo-oxidation rate of DMS in seawater.The mechanism of mercuric catalysis for DMS photolysis was suggested according to the way of CTTM(charge transfer to metal) of DMS-Hg2+ complex. | YANG Guipeng LI Chengxuan QI Jialin HU Lige HOU Haijun | 2007 | Acta Oceanologica Sinica2007，26，5： | 4 |
| 3 | Revisiting the methods for gas permeability measurement in tight porous medium显示文摘Permeability is a key parameter to describe fluid transport properties of porous medium; however, the permeability measurement is extremely difficult for tight porous medium, e.g. fine-grained rock or dense soil. In this paper, three methods for gas permeability measurement, i.e. steady state method, pulse decay method(PDM) and pressure oscillation method(POM), are first reviewed and then their advantages and drawbacks are discussed. Both analytical and numerical solutions of gas permeability are presented for the tight porous medium. The results show that the analytical method is relatively simple but only valid under certain conditions, whilst the numerical method is more robust and generic, which can take into account several factors such as porosity, saturation, gas leakage, and unconventional boundary conditions. The influence of the effective porosity on the permeability determination is further analyzed using the proposed numerical method. In this study, new pressure data interpretation procedures for PDM and POM are proposed, and the obtained results can serve as a guidance to define a proper method for permeability measurement of the tight porous medium. | Diansen Yang Wei Wang Weizhong Chen Xianjun Tan Lige Wang | 2019 | Journal of Rock Mechanics and Geotechnical Engineering2019，11，2： | 3 |
| 4 | Icariin induces osteoblast proliferation, differentiation and mineralization through estrogen receptor-mediated ERK and JNK signal activation显示文摘 | Lige Song Jiashen Zhao Xiuzhen Zhang Hong Li Yun Zhou | 2013 | European Journal of Pharmacology (-)2013，，1： | 2 |
| 5 | Experimental Study on Factors that Influence the Diameter of Dry Granulated Particles显示文摘The development of heat recovery methods for dry granulation processes from blast furnace slag in the iron and steel industry is limited because of the high consumption of granulation energy during these processes.To determine the factors that influence the diameter of granulated particles,a paraffin test platform for gas quenching granulation was established.The influences of air velocity,air flow rate,liquid mass flow rate,and liquid pipe diameter on the final particle size and mass distribution were studied.Experimental results showed that the final particle size decreased(from 1.07 mm to 0.81 mm) with increasing air velocity(from 28.3 m/s to 113.2 m/s).However,when air velocity was higher than 60 m/s,its influence on particle diameter decreased significantly.The experimental data were analyzed using SPSS Statistics software,which indicated that the effect of air velocity on particle diameter was the most significant,followed by those of air flow and liquid pipe diameter.The effect of liquid mass flow was the least significant. | TONG Lige ZHANG Pei YIN Shaowu LIU Yongxu WANG Li DING Yulong | 2016 | Journal of Thermal Science2016，25，6： | 2 |
| 6 | Bias correction of sea surface temperature retrospective forecasts in the South China Sea显示文摘Offline bias correction of numerical marine forecast products is an effective post-processing means to improve forecast accuracy. Two offline bias correction methods for sea surface temperature(SST) forecasts have been developed in this study: a backpropagation neural network(BPNN) algorithm, and a hybrid algorithm of empirical orthogonal function(EOF) analysis and BPNN(named EOF-BPNN). The performances of these two methods are validated using bias correction experiments implemented in the South China Sea(SCS), in which the target dataset is a six-year(2003–2008) daily mean time series of SST retrospective forecasts for one-day in advance, obtained from a regional ocean forecast and analysis system called the China Ocean Reanalysis(CORA),and the reference time series is the gridded satellite-based SST. The bias-correction results show that the two methods have similar good skills;however, the EOF-BPNN method is more than five times faster than the BPNN method. Before applying the bias correction, the basin-wide climatological error of the daily mean CORA SST retrospective forecasts in the SCS is up to-3°C;now, it is minimized substantially, falling within the error range(±0.5°C) of the satellite SST data. | Guijun Han Jianfeng Zhou Qi Shao Wei Li Chaoliang Li Xiaobo Wu Lige Cao Haowen Wu Yundong Li Gongfu Zhou | 2022 | Acta Oceanologica Sinica2022，41，2： | 2 |
| 7 | Positive solutions of certain elliptic systems with density dependent diffusions显示文摘 | AHN I LI Lige | 1995 | Proc Roy Soc Edinburgh Sect A1995，125，： | 1 |
| 8 | Numerical Simulation of Air-cooling Tower显示文摘A mathematic model for packed air-cooling tower thermodynamic calculation is set up in this paper on the basis of fundamental heat and mass transfer equations. Based on the Double Film theory, direct equation-solving method is used to simulate air-cooling tower, and variation of parameters is taken to analyze the data and results of the program. | Lijuan Hao Huanzhi Li Zhaohu Sun Lige TongThermal Energy Department, Beijing University of Science and Technology, Beijing 100083, China | 2003 | Journal of Thermal Science2003，12，3： | 1 |
| 9 | Icariin induces osteoblast proliferation, differentiation and mineralization through estrogen receptor-mediated ERK and JNK signal activation显示文摘 | Lige Song Jiashen Zhao Xiuzhen Zhang Hong Li Yun Zhou | 2013 | European Journal of Pharmacology (-)2013，，1： | 1 |
| 10 | Decay Properties of Er3+ Ions in Er3+:YAG and Er3+:YAlO3 显示文摘 | Huailiang Xu Lige Zhou Zhenwen Dai | 2002 | Physica B2002，，324： | 1 |
| 11 | Experimental study on filtering mixed solid-liquid dust with a sliding granular bed filter显示文摘The filtration of mixed dust that included a small number of melted(liquid)particles was studied in an experimental granular bed filter(GBF).Results show that the collection efficiency of dust containing melted particles is higher than that of dust composed of solid particles but the pressure drop from the former is higher than that of the latter.The collection efficiency and pressure drop increase as the concentration of melted particles increases.A surface sliding GBF exhibits good comprehensive performance when filtering dust,especially dust containing a mix of solid and liquid particles.The effects of the gas temperature,gas velocity,sliding filter bed thickness,and sliding collector flux on filter performance were also examined.Based on the experimental results,correlations for the collection efficiency and pressure drop for mixed dust are presented. | Yankai Peng Chuanping Liu Li Wang Shaowu Yin Lige Tong Zeyi Jiang | 2021 | Particuology2021，19，5： | 1 |
| 12 | Numerical simulation of the crack propagation processes in rocks with double joints显示文摘 | Fu Jinwei Zhu Weishen Wang Lige | | 0，，90： | 1 |
| 13 | Deficiency of a Niemann-Pick, Type Cl-related Protein in Toxoplasma Is Associated with Multiple Lipidoses and Increased Patho- genicity显示文摘 | Lige B Romano JD Bandaru VV | 2011 | PIoS Pathog2011，7，10： | 1 |
| 14 | Modeling of radiative heat transfer between high-temperature fluidized beds and immersed walls显示文摘 | Yang Jing Wang Li Tong Lige | 2004 | Chemical Engineering Science2004，59，15： | 1 |
| 15 | Liquid Air Energy Storage for Decentralized Micro Energy Networks with Combined Cooling,Heating,Hot Water and Power Supply显示文摘Liquid air energy storage(LAES)has been regarded as a large-scale electrical storage technology.In this paper,we first investigate the performance of the current LAES(termed as a baseline LAES)over a far wider range of charging pressure(1 to 21 MPa).Our analyses show that the baseline LAES could achieve an electrical round trip efficiency(e RTE)above 60%at a high charging pressure of 19 MPa.The baseline LAES,however,produces a large amount of excess heat particularly at low charging pressures with the maximum occurred at~1 MPa.Hence,the performance of the baseline LAES,especially at low charging pressures,is underestimated by only considering electrical energy in all the previous research.The performance of the baseline LAES with excess heat is then evaluated which gives a high e RTE even at lower charging pressures;the local maximum of 62%is achieved at~4 MPa.As a result of the above,a hybrid LAES system is proposed to provide cooling,heating,hot water and power.To evaluate the performance of the hybrid LAES system,three performance indicators are considered:nominal-electrical round trip efficiency(ne RTE),primary energy savings and avoided carbon dioxide emissions.Our results show that the hybrid LAES can achieve a high ne RTE between 52%and 76%,with the maximum at~5 MPa.For a given size of hybrid LAES(1 MW×8 h),the primary energy savings and avoided carbon dioxide emissions are up to 12.1 MWh and 2.3 ton,respectively.These new findings suggest,for the first time,that small-scale LAES systems could be best operated at lower charging pressures and the technologies have a great potential for applications in local decentralized micro energy networks. | SHE Xiaohui ZHANG Tongtong PENG Xiaodong WANG Li TONG Lige LUO Yimo ZHANG Xiaosong DING Yulong | 2021 | Journal of Thermal Science2021，30，1： | 1 |
| 16 | Energy storage for black start services:A review显示文摘With the increasing deployment of renewable energy-based power generation plants,the power system is becoming increasingly vulnerable due to the intermittent nature of renewable energy,and a blackout can be the worst scenario.The current auxiliary generators must be upgraded to energy sources with substantially high power and storage capacity,a short response time,good profitability,and minimal environmental concern.Difficulties in the power restoration of renewable energy generators should also be addressed.The different energy storage methods can store and release electrical/thermal/mechanical energy and provide flexibility and stability to the power system.Herein,a review of the use of energy storage methods for black start services is provided,for which little has been discussed in the literature.First,the challenges that impede a stable,environmentally friendly,and cost-effective energy storage-based black start are identified.The energy storagebased black start service may lack supply resilience.Second,the typical energy storage-based black start service,including explanations on its steps and configurations,is introduced.Black start services with different energy storage technologies,including electrochemical,thermal,and electromechanical resources,are compared.Results suggest that hybridization of energy storage technologies should be developed,which mitigates the disadvantages of individual energy storage methods,considering the deployment of energy storage-based black start services. | Yanqi Zhao Tongtong Zhang Li Sun Xiaowei Zhao Lige Tong Li Wang Jianning Ding Yulong Ding | 2022 | International Journal of Minerals,Metallurgy and Materials2022，29，4： | 1 |
| 17 | Leptin and leptin receptor abnormalities in hyperglycemic,hyperinsulinmic New-Zealand obese mice显示文摘 | Becke W Herbrg L | 1997 | Diabetes1997，105，6： | 1 |
| 18 | Novel neutrophil extracellular trap-related mechanisms in diabetic wounds inspire a promising treatment strategy with hypoxia-challenged small extracellular vesicles显示文摘Neutrophil extracellular traps(NETs)have been considered a significant unfavorable factor for wound healing in diabetes,but the mechanisms remain unclear.The therapeutic application of small extracellular vesicles(sEVs)derived from mesenchymal stem cells(MSCs)has received considerable attention for their properties.Hypoxic preconditioning is reported to enhance the therapeutic potential of MSC-derived sEVs in regenerative medicine.Therefore,the aim of this study is to illustrate the detailed mechanism of NETs in impairment of diabetic wound healing and develop a promising NET-targeting treatment based on hypoxic pretreated MSC-derived sEVs(Hypo-sEVs).Excessive NETs were found in diabetic wounds and in high glucose(HG)-induced neutrophils.Further research showed that high concentration of NETs impaired the function of fibroblasts through activating endoplasmic reticulum(ER)stress.Hypo-sEVs efficiently promoted diabetic wound healing and reduced the excessive NET formation by transferring miR-17-5p.Bioinformatic analysis and RNA interference experiment revealed that miR-17-5p in Hypo-sEVs obstructed the NET formation by targeting TLR4/ROS/MAPK pathway.Additionally,miR-17-5p overexpression decreased NET formation and overcame NET-induced impairment in fibroblasts,similar to the effects of Hypo-sEVs.Overall,we identify a previously unrecognized NET-related mechanism in diabetic wounds and provide a promising NET-targeting strategy for wound treatment. | Ziqiang Chu Qilin Huang Kui Ma Xi Liu Wenhua Zhang Shengnan Cui Qian Wei Huanhuan Gao Wenzhi Hu Zihao Wang Sheng Meng Lige Tian Haihong Li Xiaobing Fu Cuiping Zhang | 2023 | Bioactive Materials2023，，9： | 1 |
| 19 | Modeling of radiative heat transfer between high-temperature fluidized beds and immersed walls显示文摘 | YANG Jing WANG Li TONG Lige | 2004 | Chem Eng Sci2004，59，： | 1 |
| 20 | Temporal-spatial oceanic variation in relation with the three typical Kuroshio paths south of Japan显示文摘Empirical orthogonal function(EOF)analysis was applied to a 50-year long time series of monthly mean positions of the Kuroshio path south of Japan from a regional reanalysis.Three leading EOF modes characterize the contributions from three typical paths of the Kuroshio meander:the typical large meander path,the offshore nonlarge meander path,and the nearshore non-large meander path,respectively.Accordingly,the spatial variation characteristics of oceanic anomaly fields can be depicted by their regression fields upon the associated three leading principal components(PCs),which are well-matched with the results of composite analysis corresponding to each period of the three typical Kuroshio paths.A new index for the typical large meander is defined by using the second leading PC,which is highly correlated with the Kushimoto-Uragami index.Spectral analysis of this new index series shows variability of the Kuroshio path south of Japan at time scales of about 7–8 years and 20 years. | Xiaobo Wu Yanling Zhao Guijun Han Wei Li Qi Shao Lige Cao Chaoliang Li | 2022 | Acta Oceanologica Sinica2022，41，2： | 1 |
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