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| 1 | miR-126 enhances the sensitivity of non-small cell lung cancer cells to anticancer agents by targeting vascular endothelial growth factor A显示文摘增加的证据建议 hsa-miR-126 (miR-126 ) 在房间衬里的非小的房间肺癌症(NSCLC ) 是下面调整的, miR-126 的恢复由指向特定的分子损害肿瘤房间增长,移植,侵略,和幸存。这里,我们第一次报导 miR-126 涉及调整 NSCLC 房间的反应到癌症化疗。在有 miR-126 的房间模仿的 transfected A549 或禁止者以后,我们发现 miR-126 的提高的水平显著地与减少的一半被联系 adriamycin ( ADM )和长春新碱的最大的禁止的集中, ADM 的增加的累积,脉管的 endothelial 生长因素 A ( VEGFA )和 multidrug 的下面规定联系抵抗的蛋白质 1 ( MRP1 ),并且 Akt 的 inactivation 发信号的小径。而且,提高了 miR-126 的表示压制了 A549 异种皮移植的生长并且禁止了 VEGFA 和 MRP1 的表示。miR-126 能高效地通过和 VEGFA 3-untranslated 区域的相互作用的下面调整 VEGFA 表示,而 VEGFA 的恢复能部分由 miR-126 稀释 MRP1 的抑制。然而, LY294002,表明小径的 PI3K/Akt 的一个禁止者,减少这效果,建议 miR-126 的那提高的表情通过发信号的 VEGF/PI3K/Akt/MRP1 的否定规定增加了 NSCLC 房间的敏感到 anticancer 代理人小径。 | Xiaolan Zhu Hao Li Lulu Long Lulu Hui Haining Chen Xuefeng Wang Huiling Shen Wenlin Xu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，6： | 23 |
| 2 | Numerical simulation of RCS for carrier electronic warfare airplanes显示文摘This paper studies the radar cross section(RCS) of carrier electronic warfare airplanes.Under the typical naval operations section, the mathematical model of the radar wave's pitch angle incidence range analysis is established. Based on the CATIA software, considering dynamic deflections of duck wing leading edge flaps, flaperons, horizontal tail, and rudder, as well as aircraft with air-to-air missile, anti-radiation missile, electronic jamming pod, and other weapons, the 3D models of carrier electronic warfare airplanes Model A and Model B with weapons were established. Based on the physical optics method and the equivalent electromagnetic flow method, by the use of the RCSAnsys software, the characteristics of carrier electronic warfare airplanes' RCS under steady and dynamic flights were simulated under the UHF, X, and S radar bands. This paper researches the detection probability of aircraft by radars under the condition of electronic warfare, and completes the mathematical statistical analysis of the simulation results. The results show that: The Model A of carrier electronic warfare airplane is better than Model B on stealth performance and on discover probability by radar detection effectively. | Yue Kuizhi Liu Wenlin Li Guanxiong Ji Jinzu Yu Dazhao | 2015 | Chinese Journal of Aeronautics2015，28，2： | 22 |
| 3 | miR-181a sensitizes a multidrug-resistant leukemia cell line K562/A02 to daunorubicin by targeting BCL-2显示文摘这研究的目的是调查 miR-181a 是否能调制白血病的敏感药抵抗的房间衬里 K562/A02 到化学疗法的代理人 daunorubicin (DNR ) ,并且在 K562/A02 房间的 DNR 敏感上探索 miR-181a 的机制。MicroRNA microarray 和茎环颠倒抄写聚合酶链反应被用来检测 miR-181a 的表示。3-(4,5-dimethylthiazol-2-yl )-2,5-diphenyl-2H-tetrazolium 溴化物试金被执行确定 K562 房间生长和生存能力上的 miR-181a 的效果。Apoptotic 房间是用 Annexin V/FITC 和 PI apoptosis 察觉工具包检测的份量上。BCL-2 蛋白质表示被西方的污点测量。有通常认为的 BCL-2 3 untranslated 区域的酶记者向量被构造探索 BCL-2 是否是 miR-181a 的直接目标基因。BCL-2 siRNA 是进房间的 transfected 探索在 BCL-2 和 DNR 抵抗之间的关系。miR-181a 表示水平比在 K562 房间在 K562/A02 房间是更低的(P < 0.05 ) 。是有 miR-181a 禁止者的 transfected 的 K562 房间比是有 miR-181a 的 transfected 的房间模仿的 K562 房间,和 K562/A02 比 K562/A02 房间有显著地更低的幸存的有显著地更高的幸存(P < 0.05 ) 。miR-181a 能在 K562/A02 房间提高导致 DNR 的 apoptosis。BCL-2 siRNA transfected K562/A02 房间减少了幸存与 K562/A02 控制组相比。在结论, miR-181a 能在 K562/A02 房间在 DNR 抵抗的发展起一个作用, miR-181a 的在表示上能由指向 BCL-2 敏化 K562/A02 房间到 DNR。 | Hao Li Lulu Hui Wenlin Xu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，3： | 17 |
| 4 | Ghost imaging for an axially moving target with an unknown constant speed显示文摘The influence of the axial relative motion between the target and the source on ghost imaging(GI) is investigated.Both the analytical and experimental results show that the transverse resolution of GI is reduced as the deviation of the target’s center position from the optical axis or the axial motion range increases. To overcome the motion blur,we propose a deblurring method based on speckle-resizing and speed retrieval, and we experimentally validate its effectiveness for an axially moving target with an unknown constant speed. The results demonstrated here will be very useful to forward-looking GI remote sensing. | Xiaohui Li Chenjin Deng Mingliang Chen Wenlin Gong Shensheng Han | 2015 | Photonics Research2015，3，4： | 15 |
| 5 | Effects of Filler Metal Composition on Inclusions and Inclusion Defects for ER NiCrFe-7 Weldments显示文摘The effects of filler metal(FM) composition on inclusions and inclusion defects for ER NiCrFe-7 weldments have been investigated and analyzed.Results show that as Al,Ti content in FM increases from 0.14 wt%Al,0.30 wt% Ti to 0.42 wt%Al,0.92 wt%Ti,the Al,Ti reduction will increase during welding.Inclusion defects(point-like defects named by welding workers) are prone to form in the high Al,Ti content weldments.Inclusion defects with Mg,Ca,Al,and Ti as major metallic elements have been found on the surface and interior of the weldments,as Al,Ti content in FM is over 0.29 wt%Al,0.62 wt%Ti.Less Ti content in FM cannot prevent ductility-dip-cracking(DDC) through producing enough intragranular precipitates and lessening intergranular M_(23)C_6 precipitates.Nb can be used to replace Ti to reduce the sensitivity of the DDC in the NiCrFe-7 alloy weldments. | Wenlin Mo Shanping Lu Dianzhong Li Yiyi Li | 2013 | Journal of Materials Science & Technology2013，29，5： | 11 |
| 6 | Retrospective study on Professor Zhongying Zhou's experience in Traditional Chinese Medicine treatment on diabetic nephropathy显示文摘OBJECTIVE:To study and analyze the etiology and pathogenesis,diagnosis and prescription for cases of diabetic nephropathy(DN) treated by Professor Zhongying Zhou(Prof.Zhou) with the help of dada mining technique,so as to inherit his clinical experience and academic thoughts.METHODS:After pretreatment of the medical record information,statistical software SPSS 13.0 was used to analyze and process the standardized data using the descriptive analysis,cluster analysis and association rules.RESULTS:Ninety-four entries derived from Traditional Chinese Medicine(TCM) four-diagnostic information were selected.Through data mining,the highest frequency was yellow-thin-greasy tongue coating,followed by dark tongue,and then by thready-slippery pulse.The main self-conscious symptoms were limb numbness,dry mouth,frequent micturition,etc.With respect to pathogenesis,the etiology related to kidney reached 73.46%.The frequency of pathological factors for deficiency,blood stasis,heat,dampness,phlegm and dryness were 137.65%,80.25%,78.40%,48.77%,21.60%,and 14.20% respectively.A total of 236 kinds of herbs were used 2913 person times,averaging 17.98 herbs per prescription.CONCLUSION:The pathological location of DN is in the kidney.The pathogenesis of the disease is deficiency in origin and excess in superficiality,the former of which include deficiency of the liver and kidney,and deficiency of both Qi and Yin.The main pathological factors are blood stasis,heat,dampness,phlegm,and dryness;and they act upon each other to form the complex pathogenesis in terms of blood stasis-heat,dampness-heat,phlegm-heat,and dryness-heat.All the above factors are always crucial in the aggravation of DN throughout the whole course of its pathological evolution. | Kelei Su Fangshi Zhu Lizhong Guo Yao Zhu Wenlin Li Xingjiang Xiong | 2013 | Journal of Traditional Chinese Medicine2013，33，2： | 11 |
| 7 | Berberine sensitizes ovarian cancer cells to cisplatin through miR-21/PDCD4 axis显示文摘最近的研究证明了 microRNA-21 (miR-21 ) 贡献肿瘤抵抗到化疗。有趣地,我们发现了 berberine 能在几根癌症房间线禁止 miR-21 表示。在这研究,我们调查了 berberine 是否能调制卵巢的癌症房间的敏感到 cisplatin 并且探索机制。被孵化, berberine 与 cisplatin 结合了的 cisplatin 抵抗的 SKOV3 房间独自比 cisplatin 有 signicantly 更低的幸存组和提高的导致 cisplatin 的 apoptosis。Berberine 能在卵巢的癌症禁止 miR-21 表示和功能,由它的目标 PDCD4 的改进出现在卵巢的癌症的重要肿瘤 suppressor。结果建议 berberine 能经由在卵巢的癌症房间调整 miR-21/PDCD4 轴调制 cisplatin 的敏感。 | Shiguo Liu Yue Fang Huiling Shen Wenlin Xu Hao Li | 2013 | Acta Biochimica et Biophysica Sinica2013，45，9： | 9 |
| 8 | Effects of Boron on the Microstructure,Ductility-dip-cracking,and Tensile Properties for NiCrFe-7 Weld Metal显示文摘The distribution of boron and the microstructure of grain boundary(GB) precipitates(M_(23)(C,B)_6 and M_2B)have been analyzed with their effects on the susceptibility of ductility-dip-cracking(DDC) and tensile properties for NiCrFe-7 weld metal,using optical microscopy(OM),secondary ion mass spectroscopy(SIMS),scanning electron microscopy(SEM),and transmission electron microscopy(TEM).The results show that boron segregates at GBs in NiCrFe-7 weld metal during the welding process.The segregation of boron at GBs promotes the formation of continuous M_(23)(C,B)_6 carbide chains and M_2B borides along GBs.The addition of boron aggravates GB embrittlement and causes more DDC in the weld metal,by its segregation at GBs presenting as an impurity,and promoting the formation of larger and continuous M_(23)(C,B)_6 carbides,and M_2B borides along GBs.DDC in the weld metal deteriorates the ductility and tensile strength of the weld metal simultaneously. | Wenlin Mo Xiaobing Hu Shanping Lu Dianzhong Li Yiyi Li | 2015 | Journal of Materials Science & Technology2015，31，12： | 9 |
| 9 | Streamflow responses to climate change and LUCC in a semi-arid watershed of Chinese Loess Plateau显示文摘Climate change and Land Use/Cover Change(LUCC) have been identified as two primary factors affecting watershed hydrological regime. This study analyzed the trends of streamflow, precipitation, air temperature and potential evapotranspiration(PET) from 1962 to 2008 in the Jihe watershed in northwestern Loess Plateau of China using the Mann-Kendall test. The streamflow responses to climate change and LUCC were quantified independently by the elasticity method. The results show that the streamflow presented a dramatic decline with a turning point occurred in 1971, while the precipitation and PET did not change significantly. The results also show that the temperature rose markedly especially since 1990 s with an approximate increase of 1.74°C over the entire research period(1962–2008). Using land use transition matrix, we found that slope cropland was significantly converted to terrace between 1970 s and 1990 s and that forest cover increased relatively significantly because of the Grain for Green Project after 2000. The streamflow reduction was predominantly caused by LUCC and its contribution reached up to 90.2%, while the contribution of climate change to streamflow decline was only 9.8%. Although the analytical results between the elasticity method and linear regression model were not satisfactorily consistent, they both indicated that LUCC(human activity) was the major factor causing streamflow decline in the Jihe watershed from 1962 to 2008. | LI Qingyun SUN Yanwei YUAN Wenlin LYU Subing WAN Fang | 2017 | Journal of Arid Land2017，9，4： | 9 |
| 10 | miR-137 restoration sensitizes multidrug-resistant MCF-7/ADM cells to anticancer agents by targeting YB-1显示文摘到化学疗法的代理人的 Multidrug 抵抗(MDR ) 是到在乳癌病人的成功的治疗的一个主要障碍。这研究的目的是调查 miR-137 是否涉及 MDR 的规定,并且在 MCF-7/ADM 房间的敏感上探索 miR-137 的机制。miR-137 是在 MCF-7/ADM 房间的 downregulated,并且它的表达式被发现相反地在乳癌房间与 Y 盒子有约束力的 protein-1 (YB-1 ) 和 P-glycoprotein (P-gp ) 层次相关。而且, YB-1 被酶记者试金和西方的污点分析作为 miR-137 的一个目标证实。而且, miR-137 的提高的表示减少了 YB-1 和 P-gp 的蛋白质表示层次, mimicking 在到 anticancer 代理人的 MCF-7/ADM 房间的敏感击倒的 YB-1 的效果,而 YB-1 的恢复减少了这效果。在结论,我们的结果证明 miR-137 被指向 YB-1 通过 P-gp 的调整在癌症涉及 MDR,建议 miR-137 可能是为阻止并且逆行在肿瘤房间的 MDR 的一个潜在的目标。 | Xiaolan Zhu Yuefeng Li Huiling Shen Hao Li Lulu Hui Wenlin Xu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，2： | 6 |
| 11 | Effect of microwave pre-treatment on physical quality,bioactive compounds,safety risk factor,and storage stability of peanut butter显示文摘Microwave,as a new heat treatment technology,has the characteristics of uniform and fast heating speed.It is an energy-saving technology known for improving oilseed product quality.Its efficiency mainly depends on the roasting power and time.However,the production of high-quality peanut butter using short-time roasting con-ditions are limited.Herein,we determined an appropriate microwave roasting power and time for peanuts and evaluated its impacts on the quality of peanut butter.Different roasting powers(400 W,800 W and 1200 W)and times(4,4.5,5,and 5.5 min)were preliminarily tested.Among them,800 W at 5 min was the most suitable.The roasting efficiency was further evaluated using color,sensory,bioactive compounds,storage stability,and safety risk factors of peanut butter produced from four peanut cultivars(Silihong,Baisha-1016,Yuanza-9102,and Yuhua-9414).The pre-treated butter obtained from three cultivars(Silihong,Yuanza-9102,and Yuhua-9414)with moisture content between 5%and 7.2%had a similar sensory score(6-7)as the commercial on a 9-point hedonic scale compare to the other.The color of the pre-treated peanut butter varies statistically with the commercial but remained in the recommended range of Hunter L*values of 51-52,respectively,for high initial moisture peanut cultivars.The total polyphenol(35.20-31.59±0.59μmol GAE/g)and tocopherol(19.05±0.35 mg/100 g)content in the butter obtained from three cultivars(Yuahua-9102,Yuhua,and Baisha-1016)and Silihong respectively,were significantly(P<0.05)higher than those in the commercial butter.The induction times of all pre-treated butter(19.80±0.99-7.84±0.07 h)were significantly(P<0.05)longer during storage at accelerated temperature than commercial samples.In addition,no benzo[a]pyrene was found in the pre-treated samples.Collectively,the microwave pretreated peanut butter was superior to the commercial one.These findings provided data support and a reference basis to promote microwave use for peanut butter production. | Jefferson G.Degon Chang Zheng Abdeen Elkhedir Bo Yang Qi Zhou Wenlin Li | 2021 | Oil Crop Science2021，6，3： | 6 |
| 12 | miR-106a confers cisplatin resistance by regulating PTEN/Akt pathway in gastric cancer cells显示文摘最近的研究证明了 microRNA-106a (miR-106a ) 是在胃的癌症的 overexpressed 并且贡献肿瘤生长。在这研究,我们调查了 miR-106a 是否调停了到化学疗法的代理人 cisplatin (DDP ) 的胃的癌症房间线 SGC7901 的抵抗。MiR-106a 表示与它的父母线 SGC7901 相比在 DDP 抵抗房间线 SGC7901/DDP 是起来调整的。miR-106a 的 Transfection 在 SGC7901 导致了 DDP 抵抗,当在 SGC7901/DDP 的 miR-106a 的抑制导致了提高的 DDP cytotoxicity 时。进一步的学习显示 miR-106a-induced DDP 抵抗的机制包含了从染色体 10 删除的磷酸酶和 tensin 相当或相同的事物(PTEN ) 的表示蛋白质和它的下游的 phosphatidylinositol 3 kinase (PI3K )/protein kinase B (AKT ) 小径。这研究在胃的癌症提供 DDP 抵抗的新奇机制。 | Yue Fang Huiling Shen Hao Li Yuan Cao Rong Qin Lulu Long Xiaolan Zhu Chanjuan Xie Wenlin XU | 2013 | Acta Biochimica et Biophysica Sinica2013，45，11： | 6 |
| 13 | Chemical approaches to studying stem cell biology显示文摘干细胞,包括 pluripotent 干细胞和 multipotent 体的干细胞,为讯问织物开发的机制,并且为对待众多的破坏的抓住伟人潜力,动态平衡和病理疾病。建立在 vitro 平台,忠实地维持并且精确操作干细胞命运是必要的理解干细胞生物学的基本机制,并且把干细胞翻译成再生药。化学途径最近提供了能被用来控制房间自强,系区别, reprogramming 和新生的很多个小分子。这些化学调节的人被证明了是为探查干细胞生物学并且向需要的结果操作房间命运的万用的工具。最终,这策略正在答应是为针对内长的干细胞调整的药开发的新边疆。 | Wenlin Li Kai Jiang Wanguo Wei Yan Shi Sheng Ding | 2013 | Cell Research2013，23，1： | 5 |
| 14 | Pan-genome of Raphanus highlights genetic variation and introgression among domesticated,wild,and weedy radishes显示文摘Post-polyploid diploidization associated with descending dysploidy and interspecific introgression drives plant genome evolution by unclear mechanisms.Raphanus is an economically and ecologically important Brassiceae genus and model system for studying post-polyploidization genome evolution and introgres-sion.Here,we report the de novo sequence assemblies for 11 genomes covering most of the typical sub-species and varieties of domesticated,wild and weedy radishes from East Asia,South Asia,Europe,and America.Divergence among the species,sub-species,and South/East Asian types coincided with Quaternary glaciations.A genus-level pan-genome was constructed with family-based,locus-based,and graph-based methods,and whole-genome comparisons revealed genetic variations ranging from single-nucleotide polymorphisms(SNPs)to inversions and translocations of whole ancestral karyotype(AK)blocks.Extensive gene flow occurred between wild,weedy,and domesticated radishes.High fre-quencies of genome reshuffling,biased retention,and large-fragment translocation have shaped the genomic diversity.Most variety-specific gene-rich blocks showed large structural variations.Extensive translocation and tandem duplication of dispensable genes were revealed in two large rearrangement-rich islands.Disease resistance genes mostly resided on specific and dispensable loci.Variations causing the loss of function of enzymes modulating gibberellin deactivation were identified and could play an important role in phenotype divergence and adaptive evolution.This study provides new insights into the genomic evolution underlying post-polyploid diploidization and lays the foundation for genetic improve-ment of radish crops,biological control of weeds,and protection of wild species'germplasms. | Xiaohui Zhang Tongjin Liu Jinglei Wang Peng Wang Yang Qiu Wei Zhao Shuai Pang Xiaoman Li Haiping Wang Jiangping Song Wenlin Zhang Wenlong Yang Yuyan Sun Xixiang Li | 2021 | Molecular Plant2021，14，12： | 3 |
| 15 | Copper partitioning between granitic silicate melt and coexisting aqueous fluid at 850°C and 100 MPa显示文摘Experiments on the partitioning of Cu between different granitic silicate melts and the respective coexisting aqueous fluids have been performed under conditions of 850 °C, 100 MPa and oxygen fugacity(f O_2) buffered at approaching Ni–Ni O(NNO). Partition coefficients of Cu(DCu= cfluid/cmelt) were varied with different alumina/alkali mole ratios [Al_2O_3/(Na_2O·K_2O), abbreviated as Al/Alk], Na/K mole ratios, and Si O_2 mole contents. The DCu increased from 1.28 ± 0.01 to 22.18 ± 0.22 with the increase of Al/Alk mole ratios(ranging from 0.64 to 1.20)and Na/K mole ratios(ranging from 0.58 to 2.56). The experimental results also showed that DCuwas positively correlated with the HCl concentration of the starting fluid.The DCuwas independent of the Si O_2 mole content in the range of Si O_2 content considered. No DCuvalue was less than 1 in our experiments at 850 °C and 100 MPa, indicating that Cu preferred to enter the fluid phase rather than the coexisting melt phase under most conditions in the melt-fluid system, and thus a significant amount of Cu could be transported in the fluid phase in the magmatichydrothermal environment. The results indicated that Cu favored partitioning into the aqueous fluid rather than themelt phase if there was a high Na/K ratio, Na-rich, peraluminous granitic melt coexisting with the high Cl-fluid. | Shuilong Wang Hui Li Linbo Shang Xianwu Bi Xinsong Wang Wenlin Fan | 2016 | Acta Geochimica2016，35，4： | 3 |
| 16 | Influenza A virus-induced downregulation of miR-26a contributes to reduced IFNα/β production显示文摘Innate immunity provides immediate defense against viral infection. Influenza A virus(IAV) is able to get past the first line of defense. Elucidation of the molecular interaction between influenza factors and the newly recognized host players in the innate response might help in our understanding of the root causes of virulence and pathogenicity of IAV. In this study, we show that expression of miR-26 a leads to a significant inhibition of IAV replication. miR-26 a does not directly target IAV genome. Instead, miR-26 a activates the type I interferon(IFN) signaling pathway and promotes the production of IFN-stimulated genes, thus suppressing viral replication. Furthermore,ubiquitin-specific protease 3(USP3), a negative regulator of type I IFN pathway, is targeted by miR-26 a upon IAV challenge. However, miR-26 a is significantly downregulated during IAV infection.Thus, downregulation of miR-26 a is a new strategy evolved by IAV to counteract cellular antiviral responses. Our findings indicate that delivery of miR-26 a may be a potential strategy for anti-IAV therapies. | Shijuan Gao Jiandong Li Liping Song Jiaoxiang Wu Wenlin Huang | 2017 | Virologica Sinica2017，32，4： | 3 |
| 17 | Novel ACTG1 mutation causing autosomal dominant non-syndromic hearing impairment in a Chinese family显示文摘The γ-actin (ACTG1) gene is a cytoplasmic nonmuscle actin gene, which encodes a major cytoskeletal protein in the sensory hair cells of the cochlea. Mutations in ACTG1 were found to cause autosomal dominant, progressive, sensorineural hearing loss linked to the DFNA 20/26 locus on chromosome 17q25.3 in European and American families, respectively. In this study, a novel missense mutation (c.364A>G; p.I122V) co-segregated with the affected individuals in the family and did not exist in the unaffected family members and 150 unrelated normal controls. The alteration of residue Ile122 was predicted to damage its interaction with actin-binding proteins, which may cause disruption of hair cell organization and function. These findings strongly suggested that the I122V mutation in ACTG1 caused autosomal dominant non-syndromic hearing impairment in a Chinese family and expanded the spectrum of ACTG1 mutations causing hearing loss. | Ping Liu Hu Li Xiang Ren Haiyan Mao Qihui Zhu Zhengfeng Zhu Rong Yang Wenlin Yuan Jingyu Liu Qing Wang Mugen Liu | 2008 | Journal of Genetics and Genomics2008，35，9： | 3 |
| 18 | CONTROLLABILITY CANONICAL FORM FOR NONLINEAR CONTROL SYSTEMS显示文摘General nonlinear control systems are studied in this paper with the goal to transform them into the so-called controllability canonteal form via state transformation only. The conditions of transformability are given for both single input and multiple input cases. Besides, by an algebraic approach the procedure for constructing the state transformation is established. This paper is formulated in the framework of calculus rather than differential geometry approach. | Li Wenlin and Gao WeibingBeijing Universtiy of Aeronautics and Astronautics | 1990 | Chinese Journal of Aeronautics1990，5，3： | 2 |
| 19 | Vascular endothelial growth factor induces multidrug resistance-associated protein 1 overexpression through phosphatidylinositol-3-kinase/protein kinase B signaling pathway and transcription factor specificity protein 1 in BGC823 cell line显示文摘Multidrug 抵抗(MDR ) 是化疗失败和癌复发的最重要的原因之一。但是 rolesof 在 MDR 遗体的联系 MDR 的蛋白质 MRP1 糟糕理解。脉管的 endothelial 生长因素(VEGF ) , themost 之一活跃、特定的脉管的生长因素,在增长,区别,和 metastasisof 癌症起一个重要作用。在 MRP1 的表示上探索 VEGF 的效果,我们使用了 recombinant 人 VEGF 刺激 K562 andBGC-823 房间线。量的即时聚合酶链反应和西方的污点分析证明在 mRNA 和蛋白质层次的 expressionof MRP1 被增加。3-(4,5-Dimethylthiazol-2-yl )-2,5-diphenyl tetrazolium 溴化物 resultsalso 证明 VEGF 显著地提高了与 adriamycin 对待的房间的 IC 50 。探索内在的规章的机制,我们构造了 MRP1 倡导者和 theluciferase 记者基因 recombinant 向量。,酶记者基因试金证明 MRP1 倡导者的活动被 VEGF 刺激显著地增加 LY294002, phosphatidylinositol-3-kinase (PI3K ) 的一个禁止者表明小径的 /proteinkinase B (Akt ) ,减少了这效果。抄写因素特性蛋白质 1 (SP1 ) 有约束力的地点 mutationpartially 由 VEGF 堵住了 MRP1 倡导者活动的起来规定。在摘要,我们的结果表明了 MRP1 的那 VEGF enhancedthe 表情,并且表明小径和 SP1 的 PI3K/Akt 可以涉及这调整。 | Juan Li Xiaojun Wu Jinling Gong Jing Yang Jiayan Leng Qiaoyun Chen Wenlin Xu | 2013 | Acta Biochimica et Biophysica Sinica2013，45，8： | 2 |
| 20 | Non-covalent interaction between almond protein and sinapic acid: impact on protein structure and antioxidant activity显示文摘Plant phenolic acids are good sources of antioxidants and sinapic acid(SA)is one of them that can be applied in protein-based food system.However,little research is available regarding interactions between almond protein(AP)and SA.In this study,structure-affinity interaction between SA and AP,structure and antioxidant activity of proteins were investigated.Different mathematical models showed that Ka of binding SA and AP were 3.27×10^4 L/mol and 3.08×10^4 L/mol.CD(Circular dichroism)spectroscopy and FT-IR(Fourier transform infrared)spectroscopy showed that the amount of random coil andα-helix decreased whileβ-sheet increased in AP-SA complex.In combination,the interaction model of AP-SA complex was static quenching and attributed to hydrophobic interaction.Further,AP-SA complex exerted better DPPH radical scavenging ability(36.97±0.78%),ABTS+radical scavenging ability(47.26±0.45%),and higher ORAC value(2.41±0.23 M trolox/g)compared to AP.In the further,SA can be applied in protein matrix to improve film stability,gel strength and restraining fat oxidation degradation. | Xiao Jia Xin Ning Wenlin Li Qianchun Deng Xiao Yu Chen Cheng Qi Zhou | 2019 | Oil Crop Science2019，4，4： | 2 |
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