维普期刊资源整合服务平台（V6.5）

维普中文期刊产品整合服务

1篇 您的检索式：作者名="Lavinia V.Lotti"

选中清除导出查看参考文献查看引证文献引用追踪共1页首页上一页第1页下一页末页 /1 跳转

		题名	作者	年代	出处	被引量
	1	Impact of chronic exposure to bevacizumab on EpCAM-based detection of circulating tumor cells显示文摘Background:Circulating tumor cells(CTCs) are often undetected through the immunomagnetic epithelial cell adhesion molecule(EpCAM)-based CellSearch~ System in breast and colorectal cancer(CRC)patients treated with bevacizumab(BEV),where low CTC numbers have been reported even in patients with evidence of progression of disease.To date,the reasons for this discrepancy have not been clarified.This study was carried out to investigate the molecular and phenotypic changes in CRC cells after chronic exposure to BEV in vitro.Methods:The human CRC cell line WiDr was exposed to a clinically relevant dose of BEV for 3 months in vitro.The expression of epithelial and mesenchymal markers and EpCAM isoforms was determined by western blotting and immunofluorescence.To evaluate the impact of EpCAM variant isoforms expression on CTC enumeration by CellSearch~,untreated and treated colon cancer cells were spiked into 7.5 mL of blood from a healthy donor and enumerated by CellSearch~.Results:Chronic exposure of CRC cell line to BEV induced decreased expression of EpCAM 40 kDa isoform and increased expression EpCAM 42 kDa isoform,together with a decreased expression of cytokeratins(CK),while no evidence of epithelial to mesenchymal transition(EMT) in treated cells was observed.The recovery rate of cells through CellSearch~ was gradually reduced in course of treatment with BEV,being 84%,70%and 40%at 1,2 and 3 months,respectively.Conclusions:We hypothesize that BEV may prevent CellSearch~ from capturing CTCs through altering EpCAM isoforms.	Chiara Nicolazzo Isabella Massimi Lavinia V.Lotti Simone Vespa Cristina Raimondi Fabio Maria Pulcinelli Angela Gradilone Paola Gazzaniga	2015	Chinese Journal of Cancer Research2015，27，5：	1

返回顶部每页显示：

共1页首页上一页第1页下一页末页 /1 跳转

goolge学术搜索-维普

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费