5篇
您的检索式：作者名="LUO ShaoXiang"
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| 1 | Antigenic analysis of grass carp reovirus using single-chain variable fragment antibody against IgM from Ctenopharyngodon idella显示文摘Grass carp (Ctenopharyngodon idella) is an important species of freshwater aquaculture fish in China. However, grass carp reovirus (GCRV) can cause fatal hemorrhagic disease in yearling populations. Until now, a strategy to define the antigenic capacity of the virus's structural proteins for preparing an effective vaccine has not been available. In this study, some single-chain variable fragment antibodies (scFv), which could specifically recognize grass carp IgM, were selected from a constructed mouse na ve antibody phage display cDNA library. The identified scFv C1B3 clone was shown to possess relatively higher specific binding activity to grass carp IgM. Furthermore, ELISA analysis indicated that the IgM level in serum from virus-infected grass carp was more than two times higher than that of the control group at 5-7 days post infection. Moreover, Western blot analysis demonstrated that the outer capsid protein VP7 has a specific immuno-binding-reaction with the serum IgM from virus-infected grass carp. Our results suggest that VP7 can induce a stronger immune response in grass carp than the other GCRV structural proteins, which implies that VP7 protein could be used as a preferred immunogen for vaccine design. | CHEN CongLin SUN XiaoYun LIAO LanJie LUO ShaoXiang LI ZhouQuan ZHANG XiaoHua WANG YaPing GUO QionLin FANG Qin DAI HePing | 2013 | Science China(Life Sciences)2013，56，1： | 6 |
| 2 | Damage localization in plate structures from uniform load surface curvature显示文摘 | Wu Di Luo Shaoxiang | 2004 | Journal of Sound and Vibration2004，276，2： | 1 |
| 3 | Creation of the digital three-dimensional model of the prostate and its adjacent structures based on Chinese visible human显示文摘 | Wu Yi Zhang Shaoxiang Luo Na | 2010 | Surgical and Radiologic Anatomy2010，32，7： | 1 |
| 4 | Structural damage localization from modal strain energy change显示文摘 | Shi Zhiyu Luo Shaoxiang | 1998 | Journal of Sound and Vibration1998，218，5： | 1 |
| 5 | Study on peptide-peptide interaction using high-performance affinity chromatography and quartz crystal microbalance biosensor显示文摘The specific interaction between sense and antisense peptides was studied by high-performance affinity chromatography (HPAC) and quartz crystal microbalance (QCM) biosensor. Fragment 1-14 of human interferon-β (hIFN-β) was chosen as sense peptide and its three antisense peptides (AS-IFN 1, AS-IFN 2, and AS-IFN 3) were designed according to the degeneracy of genetic codes. The affinity column was prepared with sense peptide as ligand and the affinity chromatographic behavior was evaluated. Glu-substituted antisense peptide (AS-IFN 3) showed the strongest binding to immobilized sense peptide at pH 7.5. A quartz crystal microbalance-flow injection analysis (QCM-FIA) system was introduced to investigate the recognition process in real-time. The equilibrium dissociation constants between sense peptide and AS-IFN 1, AS-IFN 2 and AS-IFN 3 measured 2.08×10-4, 1.31×10-4 and 2.22× 10-5 mol/L, respectively. The mechanism study indicated that the specific recognition between sense peptide and AS-IFN 3 was due to sequence-dependent and multi-modal affinity interaction. | LUO Jia HUANG YanYan XIONG ShaoXiang LIU GuoQuan ZHAO Ruit | 2007 | Chinese Science Bulletin2007，52，10： | 1 |
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