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| 1 | A Strigolactone Biosynthesis Gene Contributed to the Green Revolution in Rice显示文摘Plant architecture is a complex agronomic trait and a major factor of crop yield,which is affected by several important hormones.Strigolactones(SLs)are identified as a new class hormoneinhibiting branching in many plant species and have been shown to be involved in various developmental processes.Genetical and chemical modulation of the SL pathway is recognized as a promising approach to modify plant architecture.However,whether and how the genes involved in the SL pathway could be utilized in breeding still remain elusive.Here,we demonstrate that a partial loss-of-function allele of the SL biosynthesis gene,HIGH TILLERING AND DWARF 1/DWARF17(HTD1/D17),which encodes CAROTENOID CLEAVAGE DIOXYGENASE 7(CCD7),increases tiller number and improves grain yield in rice.We found that the HTD1 gene had been widely utilized and co-selected with Semidwarf 1(SD1),both contributing to the improvement of plant architecture in modern rice varieties since the Green Revolution in the 1960s.Understanding how phytohormone pathway genes regulate plant architecture and how they have been utilized and selected in breeding will lay the foundation for developing the rational approaches toward improving crop yield. | Yuexing Wang Lianguang Shang Hong Yu Longjun Zeng Jiang Hu Shen Ni Yuchun Rao Sanfeng Li Jinfang Chu Xiangbing Meng Lei Wang Ping Hu Jijun Yan Shujing Kang Minghao Qu Hai Lin Tao Wang Quan Wang Xingming Hu Hongqi Chen Bing Wang Zhenyu Gao Longbiao Guo Dali Zeng Xudong Zhu Guosheng Xiong Jiayang Li Qian Qian | 2020 | Molecular Plant2020，13，6： | 23 |
| 2 | Developing disease-resistant thermosensitive male sterile rice by multiplex gene editing显示文摘High-quality and disease-resistant male sterile lines have great potential for applications in hybrid rice breeding.We introduced specific mutations into the TMS5,Pi21,and Xa13 genes in Pinzhan intermediate breeding material using the CRISPR/Cas9 multiplex genome editing system.We found that the transgenefree homozygous triple tms5/pi21/xa13 mutants obtained in the T1 generation displayed characteristics of thermosensitive genic male sterility(TGMS)with enhancedresistance to rice blast and bacterial blight.Our study provides a convenient and effective way of converting breeding intermediate material into TGMS lines through multiplex gene editing,which could significantly accelerate the breeding of sterile lines. | Sanfeng Li Lan Shen Ping Hu Qing Liu Xudong Zhu Qian Qian Kejian Wang Yuexing Wang | 2019 | Journal of Integrative Plant Biology2019，61，12： | 11 |
| 3 | The secretion of lecithinase of Pseudomonas alcaligenes S2 was via type II secretion pathway显示文摘Strain S2 is a lecithin (or phosphatidylcholine)- solubilizing bacterium, which was isolated from the rice rhizosphere in rural areas of Beijing, China. On the basis of a polyphasic study involving phenotypic tests, physiological and biochemical tests, 16S rDNA sequence analysis, G+C content determination and DNA-DNA hybridizations analy-sis, strain S2 was identified as Pseudomonas alcaligenes. P. alcaligenes S2 was mutagenized with Tn5 and four mutants showing decreased or increased solubilizing ability of lecithin were isolated based on the halo size around colonies on the solid plate supplemented with egg yolk. To characterize the genes of P. alcaligenes S2 involved in solubilization of lecithin, the EcoR I fragments of the chromosomes from the four mutant strains carrying a single transposon were cloned, and the DNA sequences flanking the Tn5 were determined. The Tn5 insertion sites in the mutants M808, M1329 and M1400, showing decreased solubilizing ability of lecithin, were found to be located in the xcpS, xcpX and xcpW , respectively, whose products XcpS, XcpX and XcpW were the components of type II secretion pathway. Complementation of xcpS, xcpX and xcpW could restore the corresponding mutants M808, M1329 and M1400 to solubilize lecithin. The data suggested that mutation in one of these xcp genes would lead to the absence of mature lecithinase secretion into the extracellular medium. The data also indicated that the secretion of leci-thin-hydrolyzing enzyme of P. alcaligenes was via type II secretion pathway. In the mutant M20 showing increasing lecithin-hydrolyzing activity, the interrupted gene showed 86% identity with chpA of Pseudomonas aeruginosa PAO1, whose product plays an important role in controlling twitch-ing motility of the bacterial cells. | LU Jing LI Fan CHEN Sanfeng LI Jilun | 2005 | Chinese Science Bulletin2005，50，16： | 3 |
| 4 | Effects of traverse speed on weld formation,microstructure and mechanical properties of ZK60 Mg alloy joint by bobbin tool friction stir welding显示文摘ZK60B(Mg-6%Zn-0.6%Zr)alloy joints fabricated by bobbin tool friction stir welding(BTFSW)with various traverse speeds were investigated.The sound joint fabricated by the BTFSW was possible under the appropriate welding parameters.The severe plastic deformation during BTFSW resulted in dispersion and segregation of the Zr-rich particles within the stirred zone(SZ)followed by evolution of a bimodal grain structure with distributed bands of 0.8-1.7μm ultrafine grains and 4.1-7.1μm equiaxed grains.Micro-hardness of SZ is substantially reduced in contrast to that of parent metal(PM)in spite of the finer grain size owing to dissolution of Mg-Zn based precipitates having hardening effects on alpha-Mg matrix.With the decrease in traverse speed,randomization degree of the plasticized metal flow increases,which is evidenced by the randomized arc line pattern at the low traverse speed.Among all defect-free joints,the 200 mm/min joint exhibits the weakest isotropy of texture within SZ and the best tensile properties,which has reduced ultimate tensile strength and yield strength by 5.4% and by 22.2%,respectively,as compared to the PM.The randomized texture hinders the joint fracturing within SZ at low elongation.Therefore,a relatively high elongation of 10.8% was achieved,which corresponded to 72% of the PM value. | Gaohui LI Li ZHOU Haifeng ZHANG Sanfeng LUO Ning GUO | 2021 | Chinese Journal of Aeronautics2021，34，12： | 3 |
| 5 | Effect of SC-CO 2 pretreatment in increasing rice straw biomass conversion显示文摘 | Miao Gao Feng Xu Shurong Li Xiaoci Ji Sanfeng Chen Dequan Zhang | 2010 | Biosystems Engineering2010，，4： | 1 |
| 6 | PAS domain of the deduced Org35 protein mediates the interaction with NifA显示文摘NifA in Azospirillum brasilense plays a key role in regulating the synthesis of nitrogenase in response to ammonia and oxygen available. Recently, our laboratory has identified four clones, whose gene prodcuts interact with NifA, from A. brasilense Sp7 genomic libraries by using the yeast two-hybrid sys- tem with NifA as bait. We are interested in clone S35, one of the four clones, because it contains a PAS-domain coding region. The entire open reading frame (ORF) for the PAS domain-containing protein was isolated and designated as org35 here. org35 gene is 2211-bp long and encodes a protein of 736 aa with a predicted molecular weight of about 78.4 kD. The predicted amino acid sequence of org35 has similarity to some two-component sensor kinase/response regulator hybrids of bacteria. Struc- tural analyses showed that Org35 comprises at least three discrete conserved domains: the N-terminal PAS, the central histidine protein kinase (HPK) and the C-terminal response regulator (RR). The PAS domain of the deduced Org35 protein was found to interact directly with NifA, but the central HPK and the C-terminal RR domains of Org35 were not. These results indicated that interaction between NifA and Org35 was mediated by PAS domain. | TU Ran CUI Yanhua CHEN Sanfeng LI Jilun | 2006 | Chinese Science Bulletin2006，51，9： | 1 |
| 7 | Chlorophyllide-a Oxygenase 1(OsCAO1) Over-Expression Affects Rice Photosynthetic Rate and Grain Yield显示文摘Leaf color and photosynthesis are important factors for rice growth and development.Hence,improving the photosynthetic rate is an effective approach for increasing rice yield.We isolated a gene,chlorophyllide-a oxygenase 1(OsCAO1),which characterized a rice near-isogenic line named fgl(faded green leaf). | HU Ping MA Jie KANG Shujing LI Sanfeng WU Xianmei ZENG Longjun LU Caolin HE Rui HE Huiying SHANG Lianguang RAO Yuchun ZHU Xudong XIONG Guosheng QIAN Qian GUO Longbiao WANG Yuexing | 2023 | Rice science2023，30，2： | 1 |
| 8 | A Method for Effectively Overcoming Tight Functional Linkage Between Genes in Rice by CRISPR/Cas9 System显示文摘Anthocyanins are widely distributed in one or more parts of rice(Oryza sativa L.)plants,including seed coat,stigma,apiculus,leaf sheath and leaf blade,and are the main pigments used in rice to achieve different colors(Hou et al,2009;Aizza and Dornelas,2011).In rice,tissue-specific color traits(especially the color of apiculus,namely the lemma and palea of the spikelet)are not only important for rice variety identification but also important for linkage analysis and rice domestication research(Saitoh et al,2004;Fan et al,2007;Lin et al,2019).The apiculus color is controlled by the complementary functions of three pairs of dominant genes,C,A and P.Gene C(chromogen)is a pigment gene,which is the basic gene for producing pigments.Gene A(activator)activates gene C,converting the chromogen into anthocyanins,and gene P(purple)controls the distribution of anthocyanins in various organs(Reddy,1996;Sakamoto et al,2001). | LI Sanfeng SHEN Lan HU Ping WU Xianmei YUAN Qiaoling RAO Yuchun QIAN Qian WANG Kejian ZHU Xudong SHANG Lianguang WANG Yuexing | 2020 | Rice science2020，27，3： | 0 |
| 9 | Microstructure,mechanical and fracture properties of friction stir welded 2195 Al-Li alloy joints显示文摘The 8 mm-thick 2195 Al-Li alloy joints were achieved by Friction Stir Welding(FSW).The microstructural evolution,temperature-dependent mechanical properties,and fracture properties were studied.The T1,δ’/β’and θ’precipitates were observed in the Base Metal(BM)and the Heat Affected Zone(HAZ).Most of the precipitates,except for re-precipitated δ’/β’phases,were dissolved in the Nugget Zone(NZ).The tensile specimens that failed at cryogenic temperatures(-196℃)had the maximum Ultimate Tensile Strength(UTS),and the fracture surface showed the inter-granular fracture characteristics.Compared to those at room temperature(25℃),the decreasing tensile properties at high temperature(180℃)were related to microstructure and strain hardening effects.The NZ presented the optimal fracture toughness,and the Crack Tip Opening Displacement(CTOD)was mutually dominated by microhardness and grain size.Analysis on Fatigue Crack Growth(FCG)rates indicates that the Thermal-Mechanically Affected Zone(TMAZ)exhibited the most superior fatigue resistance performance at stress intensity range below17 MPa.m1/2due to compressive residual stress and the crack closure effect.The fatigue fracture surfaces reveal that the crack propagation zone was characterized by the striations and secondary cracks.Also,inter-granular fracture behavior was responsible for the fastest FCG rates in the NZ. | Guangda SUN Weizhao SUN Li ZHOU Sanfeng LUO Zili ZHANG Debo LIU Huiqiang WU | 2024 | Chinese Journal of Aeronautics2024，37，1： | 0 |
| 10 | Expression, Characterization and Antimicrobial Ability of a Variant T4 Lysozyme in Pichia pastoris显示文摘T4 lysozyme was engineered with disulfide bonds and expressed in Pichia pastoris. The secreted proteins were purified and made into powder by lyophilization. Recombinant protein purity was more than 70% measured by HPLC. The lytic activity of variant T4-lysozyme was measured by the lysis of the cell wall of Xanthomonas oryzae pv. oryzae, X. oryzae pv. oryzicola, Ralstonia solanacearum comb.nov, Clavibacter michiganensis subsp. michiganensis, X. campestris pv. malvacearum, Fusarium oxysporium sp. vasinfectum, Verticillium dahliae kleb. Inhibition zone assay showed that variant T4 lysozyme significantly inhibited X. o. oryzicola and X. c. malvacearum. The antifungal activities of this protein against F. o. vasinfectum and V. d. kleb were also analyzed. | Ning SUN Sanfeng CHEN Xiangming XIE Yueju WANG Gangqiang LI Nan WANG Dehu LIU | 2014 | Agricultural Science & Technology2014，15，3： | 0 |
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