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85篇 您的检索式:作者名="Kasha"
    题名 作者 年代 出处 被引量
1转基因大麦中gfp基因的染色体位置及其表达(英文)显示文摘通过对大麦小孢子进行基因枪轰击获得 4株转绿色荧光蛋白基因 (gfp)的植株 (A、C、D、E) ,以gfp基因为探针进行荧光原位杂交 (FISH)研究转化植株中转基因插入位置和基因表达。 4个株系在染色体 7L(5HL)的不同位置都有一个插入点 ,而E株系在染色体 5S(7HS)还有第 2个插入点。所有的转基因T0 代植株都是半合子并在T1、T2代发生分离。D株系GFP未表达 ,但FISH和PCR分析表明gfp基因已成功插入其染色体。各株系在根尖和花粉中的GFP表达水平不同 :C株系在花粉表达强而在根尖表达中等 ;A株系在花粉中等表达而在根尖表达较淡 ;E株系则在根尖高表达 ,花粉中等表达。A和C株系在根尖和花粉的GFP分离都表现单位点特性 ,而E株系的根尖分离表现重叠作用 (15∶1)特征 ,但在花粉中表达GFP的频率低。PCR结果和 3个分离株系的根尖表达结果一致。D和E株系的GFP表达不正常可能和gfp基因插入位置或基因的结构有关。陈建民 Carlson A R 万建民 Kasha K J 2003Acta Genetica Sinica2003,30,8:6
2Improved plant regeneration from shed microspore culture in barley (Hordeum vulgare L.) cv. igri显示文摘A. Ziauddin E. Simion K. J. Kasha 1990Plant Cell Reports1990,,2:3
3Improvement of isolated microspore culturein wheat(Triticum aestivum L)through ovary co-culture显示文摘Hu T Kasha KJ 1997Plant Cell Rep1997,16,:1
4An improved in vitro technique for isolated microspore culture of barley显示文摘Kasha K J Simion E 2001Euphytica2001,120,3:1
5Genetic control of chromosome elimination during haploid formation in barley显示文摘HO KM KASHA KJ 1975Genetics1975,81,:1
6Emerging Patterns of Complex Technological Innovation 显示文摘Kasha D E Rycroft R 2002Technological Forecasting & Social Change2002,69,7:1
7Characterization of electronic transitions in complex molecules显示文摘Kasha M 1950Disc Faraday Soc1950,,9:1
8Selective chromosome elimination during haploid formation and amphyploid显示文摘SUBERHANYAM MC KASHA KJ 1973Pflanzenzuchtg1973,42,:1
9Studies of the chemiluminescence reaction reason of polyhydrophenols显示文摘Khan AV Kasha M 1964Nature (London)1964,204,:1
10The chemiluminescence phenomenon of sodium periodate显示文摘Khan AV Kasha MJ 1966Am Chem Soc1966,88,7:1
11Solvent effects on the electronic absorption spectrum of nitrite ion显示文摘Strickler S J Kasha M 1963Am Chem Soc1963,85,19:1
12Preparation and characterization of nanometric Mn ferrite via differ- ent methods 显示文摘AHMED M A KASHA N O EL-DEK SI 2008Nanoteehnology2008,19,06:1
13The excition model in molecular spectroscopy显示文摘KASHA M RAWLS H R EL-BAYOUMI M A 1965Pure Appl Chem1965,11,:1
14Excited state proton-transfer spectroscopy of 3-hytdroxyflavone and quercetin显示文摘Sengupta P K Kasha M 1979Chemical Physics Letters1979,68,:1
15Naturally occurring antibodies for the group B streptococcal surface immunogenic protein ( Sip ) in pregnant women and newborn babies 显示文摘Manning SD Wood S Kasha K 2006Vaccine2006,24,47:1
16The exciton model in molecular spectroscopy显示文摘Kasha M Rawls H R EI-Bayoumi M A 1965Pure Appl Chem1965,11,:1
17An improved in vitro technique for isolated microspore culture of barley显示文摘Kasha K J Simion E Oro R 2001Euphytica2001,,120:1
18The resurgence of haploids in higher plants显示文摘Forster B Heberlebors E Kasha K 2007Trends in Plant Science2007,12,8:1
19Use of haploid in breeding bar- ley显示文摘Choo T M Reinbergs E Kasha K J 1983Plant Breeding Review1983,3,:1
20Arabinogalactans and arabinogalactan-proteins induce embryogenesis in wheat (Triticum aestivum L.) microspore culture显示文摘Jocelyne Letarte Ecaterina Simion Mai Miner Ken J. Kasha 2006Plant Cell Reports2006,,12:1
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