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1篇 您的检索式:作者名="KANG Liangyi"
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1Expression of human hepatitis C virus core antigen in tobacco plants by tobacco mosaic virus-based vector system显示文摘Tobacco mosaic virus (TMV) has the potential to highly express foreign gene. A novel TMV-based in trans expression system was constructed. A TMV mutant TSHc had its coat protein replaced with hepatitis C vir黶 (HCV) core antigen gene. Anotherr TMV mutant TSBD was repli-case-defective. Coinfection of the two mutants could cause systemic infection in tobacco plants by in trans complementation of their functions. TSHc could effectively replicate and assemble to viral particles, which were a little longer than that of wild-type TMV. HCV core antigen was expressed in whole tobacco plants. A similar expression level of HCV core antigen was detected on serial passages, which suggested that this viral expression system be stable.HAN Aidong LIU Yule XIAO Li KANG Liangyi ZHANG Yuman LI Dongtian TIAN Bo 2000Chinese Science Bulletin2000,45,1:0
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