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| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | Tissue-specific transcriptional imprinting and heterogeneity in human innate lymphoid cells revealed by full-length single-cell RNA-sequencing显示文摘The impact of the microenvironment on innate lymphoid cell(ILC)-mediated immunity in humans remains largely unknown.Here we used full-length Smart-seq2 single-cell RNA-sequencing to unravel tissue-specific transcriptional profiles and heterogeneity of CD127+ILCs across four human tissues.Correlation analysis identified gene modules characterizing the migratory properties of tonsil and blood ILCs,and signatures of tissue-residency,activation and modified metabolism in colon and lung ILCs.Trajectory analysis revealed potential differentiation pathways from circulating and tissue-resident naïve ILCs to a spectrum of mature ILC subsets.In the lung we identified both CRTH2+and CRTH2−ILC2 with lung-specific signatures,which could be recapitulated by alarmin-exposure of circulating ILC2.Finally,we describe unique TCR-V(D)J-rearrangement patterns of blood ILC1-like cells,revealing a subset of potentially immature ILCs with TCR-δ rearrangement.Our study provides a useful resource for in-depth understanding of ILC-mediated immunity in humans,with implications for disease. | Luca Mazzurana Paulo Czarnewski Viktor Jonsson Leif Wigge Markus Ringnér Teresa CWilliams Avinash Ravindran Åsa KBjörklund Jesper Säfholm Gunnar Nilsson Sven-Erik Dahlén Ann-Charlotte Orre Mamdoh Al-Ameri Charlotte Höög Charlotte Hedin Sylwester Szczegielniak Sven Almer Jenny Mjösberg | 2021 | Cell Research2021，31，5： | 2 |
| 3 | Evolutionarily conserved and non-conserved retrovirus restriction activities of artiodactyl APOBEC3F proteins 显示文摘 | Jonsson S R Hache G Stenglein M D | 2006 | Nucleic Acids Res2006，34，19： | 1 |
| 4 | Oestrus detection in dairy cows from activity and lying data using on-lineindividual models显示文摘 | JONSSON R BLANKE M POULSEN IV K | 2011 | Computers & Electronics in Agriculture2011，76，1： | 1 |
| 5 | Classification cri- teria for Sjogren5 s syndrome:a revised version of the Euro- pean criteria proposed by the American-European Consensus Group显示文摘 | VITALI C BOMBARDIERI S JONSSON R MOUTSOPOULOS HM ALEXANDER EL CARSONS SE | 2002 | Ann Rheum Dis2002，61，6： | 1 |
| 6 | Measurement of costs and scales for outcome evaluation in health economic studies of Parkinson's disease 显示文摘 | Dodel R Jonsson B Reese JP | 2014 | Mov Disord2014，29，2： | 1 |
| 7 | N-acetylcysteine does not prevent bronchopulmonary dysplasia in immature infants:a randomized controlled trial显示文摘 | Lapatto R Raivio KO Selander B Stigson L Jonsson B | 2003 | J Pediatr2003，143，6： | 1 |
| 8 | Classification criteria for Sjfigren's syndrome : a revised version of the European criteria proposed by the American European Consensus Group 显示文摘 | Vitali C Bombardieri S Jonsson R | 2002 | Ann Rheum Dis2002，61，6： | 1 |
| 9 | Classification criteriafor Sjogren's syndrome:a revised version of the European criteriaproposed by the American-European Consensus Group显示文摘 | Vitali C Bombardieri S Jonsson R | 2002 | AnnRheum Dis2002，61，： | 1 |
| 10 | Detection of anti-type 3 muscarinic acetylcholine receptor autoantibodies in the sera of Sjogren 's syn- drome patients by use of a transfected cell line assay 显示文摘 | Gao J Cha S Jonsson R | 2004 | Arthritis Rheum2004，50，8： | 1 |
| 11 | Fibroblast growth factor 23 in oncogenic osteomalacia and X-linked hypophosphatemia显示文摘 | Jonsson KB Zahradnik R Larsson T White KE Sugimoto T Imanishi Y Yamamoto T Hampson G Koshiyama H Ljunggren O Oba K Yang IM Miyauchi A Econs MJ Lavigne J Juppner H | | 0，，： | 1 |
| 12 | Classification criteria for Sjsgren's syndrome: a revised version of the European criteria proposed by the American-European Consensus Group 显示文摘 | Vitali C Bombardieri S Jonsson R | 2002 | Ann Rheum Dis2002，61，： | 1 |
| 13 | Immunocompetent cells adjacent to stainless steel and titanium miniplates and screws 显示文摘 | Torgersen S Moe G Jonsson R | 1995 | Eur J Oral Sci1995，103，1： | 1 |
| 14 | Revisiting Sjogren' s syndrome in the new millennium:perspectives on assessment and outcome measures显示文摘 | Bowman SJ Pillemer S Jonsson R | 2001 | Rheumatology2001，40，10： | 1 |
| 15 | High mobility group box chromosomal protein1,a DNA binding cytokine,induces arthritis显示文摘 | Pullerits R Jonsson I M Verdrengh M | 2003 | Arthritis Rheum2003，48，6： | 1 |
| 16 | Work capacity and central hemodynamics thirteen to twenty-six years after repair of tetralogy of Fallot 显示文摘 | Jonsson H Ivert T Jonasson R | 1995 | J Thorac Cardiovasc Surg1995，110，： | 1 |
| 17 | The influence of a low-molecular hydrophobic solute on the flux of polysuphone ultrafiltration membranes with different cut-off 显示文摘 | Lindau J Jonsson A S Wimmerstedt R | 1995 | J Membr Sci1995，106，： | 1 |
| 18 | Apoptosis in oral lichen planus显示文摘 | Johannessen AC Jonsson R | 2001 | Eur J Oral Sci2001，109，5： | 1 |
| 19 | Classification criteria for Sjogren's syndrome: a revised version of the European criteria proposed by the American- European Consensus Group显示文摘 | Vitali C Bombardieri S Jonsson R | 2002 | Ann Rheum Dis2002，61，： | 1 |
| 20 | Classica- tion criteria for Sjogren's syndrome: a revised version of the European criteria proposed by the American Eu- ropean Consensus Group显示文摘 | Vitali C Bom bardieri S Jonsson R | 2002 | Ann Rheum Dis2002，61，： | 1 |
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