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| 1 | Herbal decoctosome is a novel form of medicine显示文摘Traditionally, herbal medicine is consumed by drinking decoctions produced by boiling herbs with water. The functional components of the decoction are heat stable. Small RNAs(sRNAs) were reported as a new class of functional components in decoctions. However, the mechanisms by which sRNAs survive heat treatment of the decoction and enter cells are unclear.Previous studies showed that plant-derived exosome-like nanoparticles(ELNs), which we call botanosomes, could deliver therapeutic reagents in vivo. Here, we report that heat-stable decoctosomes(ELNs) from decoctions have more therapeutic effects than the decoctions in vitro and demonstrate therapeutic efficacy in vivo. Furthermore, sRNAs, such as HJT-sRNA-m7 and PGY-sRNA-6, in the decoctosome exhibit potent anti-fibrosis and anti-inflammatory effects, respectively. Decoctosome is comprised of lipids, chemical compounds, proteins, and s RNAs. A medical decoctosome mimic is called bencaosome. A single lipid sphinganine(d22:0) identified in the decoctosome was mixed and heated with the synthesized sRNAs to form the simplest bencaosome. This simple bencaosome structure was identified by critical micelle concentration(cmc) assay that sRNAs coassembled with sphinganine(d22:0) to form the lipid layers of vesicles. The heating process facilitates co-assembly of sRNAs and sphinganine(d22:0) until a steady state is reached. The artificially produced sphinganine-HJT-sRNA-m7 and sphinganinePGY-sRNA-6 bencaosomes could ameliorate bleomycin-induced lung fibrosis and poly(I:C)-induced lung inflammation, respectively, following oral administration in mice. Our study not only demonstrates that the herbal decoctosome may represent a combinatory remedy in precision medicine but also provides an effective oral delivery route for nucleic acid therapy. | Xiaoyun Li Zhu Liang Jianchao Du Zhiqing Wang Song Mei Zhiqing Li Yan Zhao DANDan Zhao Yiming Ma Jun Ye Jiantao Xu Yu Zhao Jiahui Chang Yuhao Qin Lanlan Yu Chenxuan Wang Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 26 |
| 2 | The antitumor effect of tanshinone IIA on antiproliferation and decreasing VEGF/VEGFR2 expression on the human non-small cell lung cancer A549 cell line显示文摘The effects of tanshinone IIA on the proliferation of the human non-small cell lung cancer cell line A549 and its possible mechanism on the VEGF/VEGFR signal pathway were investigated.The exploration of the interaction between tanshinone IIA and its target proteins provides a feasible platform for studying the anticancer mechanism of active components of herbs.The CCK-8 assay was used to evaluate the proliferative activity of A549 cells treated with tanshinone IIA(2.5 80 μmol/L) for 24,48 and 72 h,respectively.Flow cytometry was used for the detection of cell apoptosis and cell cycle perturbation.VEGF and VEGFR2 expression were studied by Western blotting.The binding mode of tanshinone IIA within thecrystal structure of the VEGFR2 protein was evaluated with molecular docking analysis by use of the CDOCKER algorithm in Discovery Studio 2.1.The CCK-8 results showed that tanshinone IIA can significantly inhibit A549 cell proliferation in a dose-and time-dependent manner.Flow cytometry results showed that the apoptosis rate of tested group was higher than the vehicle control,and tanshinone IIAtreated cells accumulated at the S phase,which was higher than the vehicle control.Furthermore,the expression of VEGF and VEGFR2 was decreased in Western blot.Finally,molecular docking analysis revealed that tanshinone IIA could be stably docked into the kinase domain of VEGFR2 protein with its unique modes to form H-bonds with Cys917 and π–π stacking interactions with Val848.In conclusion,tanshinone IIA may suppress A549 proliferation,induce apoptosis and cell cycle arrest at the S phase.This drug may suppress angiogenesis by targeting the protein kinase domains of VEGF/VEGFR2. | Jun Xie Jiahui Liu Heng Liu Shihui Liang Meigui Lin Yueyu Gu Taoli Liu Dongmei Wang Hui Gee Sui-lin Mo | 2015 | Acta Pharmaceutica Sinica B2015，5，6： | 26 |
| 3 | Plant-derived phosphocholine facilitates cellular uptake of anti-pulmonary fibrotic HJT-sRNA-m7显示文摘Pulmonary fibrosis, a progressive chronic disease with a high mortality rate, has limited treatment options. Currently, lung transplantation remains the only effective treatment. Here we report that a small RNA, HJT-sRNA-m7, from a Chinese herbal medicine Hong Jing Tian(HJT, RHODIOHAE CRENULATAE RADIX ET RHIZOMA, Rhodiola crenulata) can effectively reduce the expressions of fibrotic hallmark genes and proteins both in alveolar in vitro and in mouse lung tissues in vivo. We also discovered over one hundred oil-soluble chemicals from HJT decoctions, most of which are found in lipid extracts from other Chinese herbals decoctions, including Pu Gong Ying(PGY, TARAXACI HERBA, Taraxacum mongolicum), Chuan Xin Lian(CXL, changed to 'ANDROGRAPHIS HERBA, Andrographis paniculata'), and Jin Yin Hua(JYH, lonicera japonica or Honeysuckle). We identified the active component in these decoctions as two forms of phosphocholines, PC(18:0/18:2) and PC(16:0/18:2). These PCs potentially could form liposomes with small RNAs to enter human alveolar and gastric cells. Our experimental results suggest an unprecendent lipid complex route through which botanic small RNA can enter human bodies.Our results provide an innovative treatment strategy for oral delivery of siRNAs as therapeutic medication. | Jianchao Du Zhu Liang Jiantao Xu Yan Zhao Xiaoyun Li Yanli Zhang DANDan Zhao Ruxuan Chen Yang Liu Trupti Joshi Jiahui Chang Zhiqing Wang Yanxu Zhang Jindong Zhu Qiang Liu Dong Xu Chengyu Jiang | 2019 | Science China(Life Sciences)2019，62，3： | 21 |
| 4 | The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility显示文摘Objective: To examine the effect of pSer9-GSK-3β on breast cancer and to determine whether the underlying metabolic and immunological mechanism is associated with ROS/eIF2B and natural killer(NK) cells.Methods: We employed TWS119 to inactivate GSK-3β by phosphorylating Ser9 and explored its effect on breast cancer and NK cells. The expression of GSK-3β, natural killer group 2 member D(NKG2D) ligands, eIF2B was quantified by PCR and Western blot. We measured intracellular reactive oxygen species(ROS) and mitochondrial ROS using DCFH-DA and MitoSOX^(TM) probe,respectively, and conducted quantitative analysis of cellular respiration on 4T1 cells with mitochondrial respiratory chain complex Ⅰ/Ⅲ kits.Results: Our investigation revealed that TWS119 downregulated NKG2D ligands(H60 a and Rae1), suppressed the cytotoxicity of NK cells, and promoted the migration of 4T1 murine breast cancer cells. Nevertheless, LY290042, which attenuates p-GSK-3β formation by inhibiting the PI3K/Akt pathway, reversed these effects. We also found that higher expression of p Ser9-GSK-3β induced higher levels of ROS, and observed that abnormality of mitochondrial respiratory chain complex Ⅰ/Ⅲ function induced the dysfunction of GSK-3β-induced electron transport chain, naturally disturbing the ROS level. In addition, the expression of NOX3 and NOX4 was significantly up-regulated, which affected the generation of ROS and associated with the metastasis of breast cancer. Furthermore, we found that the expression of pSer535-eIF2B promoted the expression of NKG2D ligands(Mult-1 and Rae1) following by expression of pSer9-GSK-3β and generation of ROS.Conclusions: The PI3K/Akt/GSK-3β/ROS/eIF2B pathway could regulate NK cell activity and sensitivity of tumor cells to NK cells,which resulted in breast cancer growth and lung metastasis. Thus, GSK-3β is a promising target of anti-tumor therapy. | Fengjiao Jin Zhaozhen Wu Xiao Hu Jiahui Zhang Zihe Gao Xiao Han Junfang Qin Chen Li Yue Wang | 2019 | Cancer Biology & Medicine2019，16，1： | 20 |
| 5 | Sirt1-ROS-TRAF6 Signaling-Induced Pyroptosis Contributes to Early Injury in Ischemic Mice显示文摘Stroke is an acute cerebro-vascular disease with high incidence and poor prognosis,most commonly ischemic in nature.In recent years,increasing attention has been paid to inflammatory reactions as symptoms of a stroke.However,the role of inflammation in stroke and its underlying mechanisms require exploration.In this study,we evaluated the inflammatory reactions induced by acute ischemia and found that pyroptosis occurred after acute ischemia both in vivo and in vitro,as determined by interleukin-1β,apoptosis-associated speck-like protein,and caspase-1.The early inflammation resulted in irreversible ischemic injury,indicating that it deserves thorough investigation.Meanwhile,acute ischemia decreased the Sirtuin 1(Sirtl)protein levels,and increased the TRAF6(TNF receptor associated factor 6)protein and reactive oxygen species(ROS)levels.In further exploration,both Sirtl suppression and TRAF6 activation were found to contribute to this pyroptosis.Reduced Sirtl levels were responsible for the production of ROS and increased TRAF6 protein levels after ischemic exposure.Moreover,N-acetyl-L-cysteine,an ROS scavenger,suppressed the TRAF6 accumulation induced by oxygen-glucose deprivation via suppression of ROS bursts.These phenomena indicate that Sirtl is upstream of ROS,and ROS bursts result in increased TRAF6 levels.Further,the activation of Sirtl during the period of ischemia reduced ischemiainduced injury after 72 h of reperfusion in mice with middle cerebral artery occlusion.In sum,these results indicate that pyroptosis-dependent machinery contributes to the neural injury during acute ischemia via the Sirt1-ROS-TRAF6 signaling pathway.We propose that inflammatory reactions occur soon after oxidative stress and are detrimental to neuronal survival;this provides a promising therapeutic target against ischemic injuries such as a stroke. | Weijie Yan Wei Sun Jiahui Fan Haiqing Wang Song Han Junfa Li Yanling Yin | 2020 | Neuroscience Bulletin2020，36，8： | 9 |
| 6 | An Optimized Random Forest Model and Its Generalization Ability in Landslide Susceptibility Mapping:Application in Two Areas of Three Gorges Reservoir,China显示文摘Numerous researches have been published on the application of landslide susceptibility assessment models;however,they were only applied in the same areas as the models were originated,the effect of applying the models to other areas than the origin of the models has not been explored.This study is purposed to develop an optimized random forest(RF)model with best ratios of positive-to-negative cells and 10-fold cross-validation for landslide susceptibility mapping(LSM),and then explore its generalization ability not only in the area where the model is originated but also in area other than the origin of the model.Two typical counties(Fengjie County and Wushan County)in the Three Gorges Reservoir area,China,which have the same terrain and geological conditions,were selected as an example.To begin with,landslide inventory was prepared based on field investigations,satellite images,and historical records,and 1522 landslides were then identified in Fengjie County.22 landslide-conditioning factors under the influence of topography,geology,environmental conditions,and human activities were prepared.Then,combined with 10-fold cross-validation,three typical ratios of positive-to-negative cells,i.e.,1:1,1:5,and 1:10,were adopted for comparative analyses.An optimized RF model(Fengjie-based model)with the best ratios of positive-to-negative cells and 10-fold cross-validation was constructed.Finally,the Fengjie-based model was applied to Fengjie County and Wushan County,and the confusion matrix and area under the receiver operating characteristic(ROC)curve value(AUC)were used to estimate the accuracy.The Fengjie-based model delivered high stability and predictive capability in Fengjie County,indicating a great generalization ability of the model to the area where the model is originated.The LSM in Wushan County generated by the Fengjie-based model had a reasonable reference value,indicating the Fengjiebased model had a great generalization ability in area other than the origin of the model.The Fengjiebased model in this study could be applied in other similar areas/countries with the same terrain and geological conditions,and a LSM may be generated without collecting landslide information for modeling,so as to reduce workload and improve efficiency in practice. | Deliang Sun Jiahui Xu Haijia Wen Yue Wang | 2020 | Journal of Earth Science2020，31，6： | 8 |
| 7 | Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization显示文摘Three-dimensional(3D)printing has been increasingly employed to produce advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths,in order to induce improved bone regeneration in defects with a critical size.Given that the successful bone regeneration requires both excellent osteogenesis and vascularization,endowing scaffolds with both strong bone forming ability and favorable angiogenic potential would be highly desirable to induce improved bone regeneration with required vascularization.In this investigation,customized bone tissue engineering scaffolds with balanced osteoconductivity/osteoinductivity were produced via cryogenic 3D printing ofβ-tricalcium phosphate and osteogenic peptide(OP)containing water/poly(lactic-co-glycolic acid)/dichloromethane emulsion inks.The fabricated scaffolds had a hierarchically porous structure and were mechanically comparable to human cancellous bone.Angiogenic peptide(AP)containing collagen I hydrogel was then coated on scaffold surface to further provide scaffolds with angiogenic capability.A sequential release with a quick AP release and a slow but sustained OP release was obtained for the scaffolds.Both rat endothelial cells(ECs)and rat bone marrow derived mesenchymal stem cells(MSCs)showed high viability on scaffolds.Improved in vitro migration and angiogenesis of ECs were obtained for scaffolds delivered with AP while enhanced osteogenic differentiation was observed in scaffolds containing OP.The in vivo results showed that,toward scaffolds containing both AP and OP,the quick release of AP induced obvious angiogenesis in vivo,while the sustained OP release significantly improved the new bone formation.This study provides a facile method to produce dual-delivery scaffolds to achieve multiple functions. | Chong Wang Jiahui Lai Kai Li Shaokui Zhu Bingheng Lu Jia Liu Yujin Tang Yen Wei | 2021 | Bioactive Materials2021，6，1： | 7 |
| 8 | Solvent-free nanocasting toward universal synthesis of ordered mesoporous transition metal sulfide@N-doped carbon composites for electrochemical applications显示文摘Transition metal sulfides (TMSs) have a wide range of applications owing to their intriguing properties.Significant efforts have been devoted to nanostructuring TMSs to enhance their properties and performance,still there is a high need in general synthesis of TMS nanostructures.Herein,for the first time,a simple solvent free reactive nanocasting approach that integrates solid precursor loading,in-situ sulfuration and carbonization into a single heating step is developed for the universal synthesis of ordered mesoporous TMS@N-doped carbon composites (denoted as OM-TMS@NCs) with methionine (Met) and metal chlorides as the precursors and the mesoporous silica (SBA-15) as the hard template.A series of OM-TMS@NCs with a hexagonal mesostructure,ultra-high surface areas (430-754 m2·g-1),large pore volumes (0.85-1.32 cm3·g-1),and unique TMS stoichiometries,including MoS2,Fe7S8,Co9S8,NiS,Cu7S4 and ZnS,are obtained.Two distinct structure configurations,namely,highly dispersed ultrathin TMS nanosheets within NCs and TMS@NC co-nanowire arrays,can be obtained depending on different metals.The structure evolution of the OM-TMS@NCs over the solvent-free nanocasting process is studied in detail for a deep understanding of the synthesis.As demonstrations,these materials are promising for electrocatalytic hydrogen evolution reaction and lithium ion storage with high performances. | Jiahui Zhu Zhi Chen Lin Jia Yuqi Lu Xiangru Wei Xiaoning Wang Winston Duo Wu Na Han Yanguang Li Zhangxiong Wu | 2019 | Nano Research2019，12，9： | 5 |
| 9 | Research on Mobile Internet Mobile Agent System Dynamic Trust Model for Cloud Computing显示文摘This paper analyzes the reasons for the formation of security problems in mobile agent systems, and analyzes and compares the security mechanisms and security technologies of existing mobile agent systems from the perspective of blocking attacks. On this basis, the host protection mobile agent protection technology is selected, and a method to enhance the security protection of mobile agents (referred to as IEOP method) is proposed. The method first encrypts the mobile agent code using the encryption function, and then encapsulates the encrypted mobile agent with the improved EOP protocol IEOP, and then traces the suspicious execution result. Experiments show that using this method can block most malicious attacks on mobile agents, and can protect the integrity and confidentiality of mobile agents, but the increment of mobile agent tour time is not large. | Weijin Jiang Yang Wang Yirong Jiang Jiahui Chen Yuhui Xu Lina Tan | 2019 | China Communications2019，16，7： | 5 |
| 10 | Nuclear miR-665 aggravates heart failure via suppressing phosphatase and tensin homolog transcription显示文摘Although numerous miRNAs have been discovered,their functions in the different subcellular organelles have remained obscure.In this study,we found that miR-665 was enriched in the nucleus of cardiomyocytes,and then investigated the underlying role of nuclear miR-665 in heart failure.RNA fluorescence in situ hybridization assays in human heart tissue sections and primary cardiomyocytes showed that miR-665 was localized in the nucleus of cardiomyocytes.Increased expression of nuclear miR-665 was observed not only in the cardiomyocytes isolated from the heart of mice treated in vivo by transverse aortic constriction(TAC),but also in phenylephrine(PE)-treated cultured cardiomyocytes in vitro.To further explore the role of miR-665 in heart failure,a type 9 recombinant adeno-associated virus(rAAV)system was employed to manipulate the expression of miR-665 in mice.Overexpression of miR-665 aggravated TAC-induced cardiac dysfunction,while down-expression of miR-665 showed opposite effects.Bioinformatic prediction and biological validation confirmed that the PTEN(phosphatase and tensin homolog)gene was one of the targets of miR-665 in the nucleus.Furthermore,restoring PTEN expression significantly eliminated the destructive effects of miR-665 over-expression in TAC-induced cardiac dysfunction.Our data showed that nuclear miR-665 aggravates heart failure via inhibiting PTEN expression,which provided a therapeutic approach for heart failure. | Jiahui Fan Xudong Zhang Xiang Nie Huaping Li Shuai Yuan Beibei Dai Jiabing Zhan Zheng Wen Jiangang Jiang Chen Chen Daowen Wang | 2020 | Science China(Life Sciences)2020，63，5： | 4 |
| 11 | The double face of miR-320:cardiomyocytes-derived miR-320 deteriorated while fibroblasts-derived miR-320 protected against heart failure induced by transverse aortic constriction显示文摘MicroRNAs(miRNAs)are aberrantly expressed in the pathophysiologic process of heart failure(HF).However,the functions of a certain miRNA in different cardiac cell types during HF are scarcely reported,which might be covered by the globe effects of it on the heart.In the current study,Langendorff system was applied to isolate card io myocytes(CMs)and cardiac fibroblasts(CFs)from transverse aortic constriction(TAC)-induced mice.Slight increase of miR-320 expression was observed in the whole heart tissue of TAC mice.Interestingly,miR-320 was significantly elevated in CMs but decreased in CFs from TAC mice at different time points.Then,recombinant adeno-associated virus 9 with cell-type-specific promoters were used to manipulate miR-320 expressions in vivo.Both in vitro and in vivo experiments showed the miR-320 overexpression in CMs exacerbated cardiac dysfunction,whereas overexpression of miR-320 in CFs alleviated cardiac fibrosis and hypertrophy.Mechanically,downstream signaling pathway analyses revealed that miR-320 might induce various effects via targeting PLEKHM3 and IFITM1 in CMs and CFs,respectively.Moreover,miR-320 mediated effects could be abolished by PLEKHM3 re-expression in CMs or IFITM1 re-expression in CFs.Interestingly,miR-320 treated CFs were able to indirectly affect CMs function,but not vice versa.Meanwhile,upstream signaling pathway analyses showed that miR-320 expression and decay rate were rigorously manipulated by Ago2,which was regulated by a cluster of cell-type-specific TFs distinctively expressed in CMs and CFs,respectively.Together,we demonstrated that miR-320 functioned differently in various cell types of the heart during the progression of HF. | Xudong Zhang Shuai Yuan Huaping Li Jiabing Zhan Feng Wang Jiahui Fan Xiang Nie Yan Wang Zheng Wen Yanghui Chen Chen Chen Dao Wen Wang | 2021 | Signal Transduction and Targeted Therapy2021，6，3： | 4 |
| 12 | National-scale data-driven rainfall induced landslide susceptibility mapping for China by accounting for incomplete landslide data显示文摘China is one of the countries where landslides caused the most fatalities in the last decades. The threat that landslide disasters pose to people might even be greater in the future, due to climate change and the increasing urbanization of mountainous areas. A reliable national-scale rainfall induced landslide susceptibility model is therefore of great relevance in order to identify regions more and less prone to landsliding as well as to develop suitable risk mitigating strategies. However, relying on imperfect landslide data is inevitable when modelling landslide susceptibility for such a large research area. The purpose of this study is to investigate the influence of incomplete landslide data on national scale statistical landslide susceptibility modeling for China. In this context, it is aimed to explore the benefit of mixed effects modelling to counterbalance associated bias propagations. Six influencing factors including lithology, slope,soil moisture index, mean annual precipitation, land use and geological environment regions were selected based on an initial exploratory data analysis. Three sets of influencing variables were designed to represent different solutions to deal with spatially incomplete landslide information: Set 1(disregards the presence of incomplete landslide information), Set 2(excludes factors related to the incompleteness of landslide data), Set 3(accounts for factors related to the incompleteness via random effects). The variable sets were then introduced in a generalized additive model(GAM: Set 1 and Set 2) and a generalized additive mixed effect model(GAMM: Set 3) to establish three national-scale statistical landslide susceptibility models: models 1, 2 and 3. The models were evaluated using the area under the receiver operating characteristics curve(AUROC) given by spatially explicit and non-spatial cross-validation. The spatial prediction pattern produced by the models were also investigated. The results show that the landslide inventory incompleteness had a substantial impact on the outcomes of the statistical landslide susceptibility models. The cross-validation results provided evidence that the three established models performed well to predict model-independent landslide information with median AUROCs ranging from 0.8 to 0.9.However, although Model 1 reached the highest AUROCs within non-spatial cross-validation(median of 0.9), it was not associated with the most plausible representation of landslide susceptibility. The Model 1 modelling results were inconsistent with geomorphological process knowledge and reflected a large extent the underlying data bias. The Model 2 susceptibility maps provided a less biased picture of landslide susceptibility. However, a lower predicted likelihood of landslide occurrence still existed in areas known to be underrepresented in terms of landslide data(e.g., the Kuenlun Mountains in the northern Tibetan Plateau). The non-linear mixed-effects model(Model 3) reduced the impact of these biases best by introducing bias-describing variables as random effects. Among the three models, Model 3 was selected as the best national-scale susceptibility model for China as it produced the most plausible portray of rainfall induced landslide susceptibility and the highest spatially explicit predictive performance(median AUROC of spatial cross validation 0.84) compared to the other two models(median AUROCs of 0.81 and 0.79, respectively). We conclude that ignoring landslide inventory-based incompleteness can entail misleading modelling results and that the application of non-linear mixed-effect models can reduce the propagation of such biases into the final results for very large areas. | Qigen Lin Pedro Lima Stefan Steger Thomas Glade Tong Jiang Jiahui Zhang Tianxue Liu Ying Wang | 2021 | Geoscience Frontiers2021，12，6： | 4 |
| 13 | Dynamical decoupling of electron spins in phosphorus-doped silicon显示文摘Quantum coherence is an important enabling feature underpinning quantum computation. However, because of couplings with its noisy surrounding environment, qubits suffer from the decoherence effects. The dynamical decoupling (DD) technique uses pulse-induced qubit flips to effectively mitigate couplings between qubits and environment. Optimal DD eliminates dephasing up to a given order with the minimum number of pulses. In this paper, we first introduce our recent work on prolonging electron spin coherence in γ-irradiated malonic acid crystals and analyze different decoherence mechanisms in this solid system. Then we focus on electron spin relaxation properties in another system, phosphorous-doped silicon (Si:P) crystals. These properties have been investigated by pulse electron paramagnetic resonance (EPR). We also investigate the performance of the dynamical decoupling technique on this system. Using 8-pulse periodic DD, the coherence time can be extended to 296 μs compared with 112 μs with one-pulse control. | RONG Xing WANG Ya YANG JiaHui ZHU JinXian XU WanJie FENG PengBo WEN XuJie SU JiHu DU JiangFeng | 2011 | Chinese Science Bulletin2011，56，7： | 4 |
| 14 | Trimetallic PtRhNi alloy nanoassemblies as highly active electrocatalyst for ethanol electrooxidation显示文摘尽管 nanostructures 广泛地基于高贵金属合金被利用在(电镀物品) 催化作用,他们的低温度的合成由于金属先锋的不同 Nernst 平衡潜力仍然是庞大的挑战。此处,我们用一个简单混合 cyanogel 减小方法与悦耳的 Pt/Rh 比率描述 trimetallic PtRhNi 合金 nanoassemblies (PtRhNi 言论集) 的成功的合成并且提供他们的化学作文,形态学,和结构的详细描述。另外, PtRhNi 言论集的电气化学的性质被周期的 voltammetry 检验,揭示在乙醇氧化反应(EOR ) 的作文依赖者 electrocatalytic 活动。比作商业磅黑人 electrocatalyst,优化的磅 3 Rh 1 Ni 2-ANAs 显示显著地在碱的媒介提高了 EOR electrocatalytic 性能。 | Huimin Liu Jiahui Li Lijuan Wang Yawen Tang Bao Yu Xia Yu Chen | 2017 | Nano Research2017，10，10： | 4 |
| 15 | Molecular Characteristics and Phylogenetic Analysis of N Gene of Human Derived Rabies Virus显示文摘Objective To investigate the relationship between the molecular characteristics and phylogenetic evolution of rabies N gene.Methods Saliva samples were collected from rabies cases,and RT-PCR was used to amplify the N gene of rabies virus with the specific primers.The amplifying product of RT-PCR was cloned to pUCm-T vector and transformed into E.coli XL1-Blue and then the blue-white selection,PCR screening and gene sequencing were carried out to identify the positive clones.Finally,ExPASy and other bioinformatics softwares were used to analyze and predict the structure and biological characteristics of the N genome.Results The amplification product of RT-PCR was 1 353 bp,the recombinant plasmid pUCm-T/N was constructed,the whole length of the N gene open reading frame was composed of 1 353 nucleotide residues to code 450 amino acids (20 kinds),the accession number submitted to the Genbank was HM756692,its sequence homology of nucleotides and amino acids compared with the vaccine strain CTN-1-V was 90% and 99% respectively.The evolutionary analysis showed that the isolated strain belonged to genotype I with certain geographic regionality.Conclusion The characteristics investigation and bioinformatics analysis of Hunan0806 N gene will provide fundament data to reveal the significance of the N gene characteristics for rabies epidemiology and its prevention & control. | CAI Liang TAO XiaoYan LIU YunZhi ZHANG Hong GAO LiDong HU ShiXiong LIU FuQiang LI Hao SHEN XinXin LIU JiaHui WANG ShiQing TANG Qing | 2011 | Biomedical and Environmental Sciences2011，24，4： | 4 |
| 16 | A fluorescent Eu(Ⅲ) MOF for highly selective and sensitive sensing of picric acid显示文摘A metal-organic framework [Eu_3L_3(CH_3COO)_2(H_2O)_2(μ_3-OH)]·3 DMF,(EuL, H_2L=9H-carbazole-2,7-dicarboxylic acid,DMF=N,N-dimethylformamide) has been synthesized under solvothermal conditions and structurally characterized. In EuL,Eu_6O_8 clusters are four-bridged by carboxylates to form parallel-aligned Eu–O–C chains, which are further linked by the carbazole moieties of L^(2-) ligands to form the three-dimensional framework with rhombic channels. The EuL material with characteristic emission of Eu^(3+) ion exhibits significant luminescence quenching response for picric acid(PA) and the linear Stern-Volmer plot was observed in the concentration range of 0.05–0.15 mM with K_(sv) of 98074 M^(-1). As far as we know, this Ksv is among the highest values for COFs and MOFs in detection of PA. The excellent anti-interference ability and repeatability were also verified by experiments. Lastly, we investigated the luminescence quenching mechanism in the EuL sensing system. | Qianqian Chen Jiahui Cheng Jin Wang Liang Li Zhipeng Liu Xinhui Zhou Yujian You Wei Huang | 2019 | Science China Chemistry2019，62，2： | 3 |
| 17 | Genome-Wide Identification, Characterization, and Expression Analysis of Calmodulin-Like Proteins(CMLs) in Apple显示文摘Calcium(Ca^(2+)), a crucial second messenger in plants, is involved in diverse signaling pathways including biotic and abiotic stress responses. However, the functions of most calcium sensors including calcium-dependent protein kinases(CDPKs), calmodulins(Ca Ms), calmodulinlike proteins(CMLs), and calcineurin B-like proteins(CBLs) remain limited in plants, particularly in woody plants. Currently, a total of 83 CMLs and seven Ca Ms were discovered in the apple genome in this study. Functional domain analysis showed that these CMLs and Ca Ms contain a highly conserved EF-hand motif. q RT-PCR assays indicated that these CMLs were expressed ubiquitously in apple, including in the roots, stems,leaves, flowers, and fruits, and also possessed spatial specificity. Finally, most of these CMLs were induced by hormonal and abiotic stress, suggesting their potential roles in the regulation of growth, development, and the stress response in apple. In summary, our findings provide potential evidence that apple CMLs may be involved in abiotic stress and the regulation of plant growth and development. | ZHANG Quanyan LIU Xiaojuan LIU Xin WANG Jiahui YU Jianqiang HU Dagang HAO Yujin | 2017 | Horticultural Plant Journal2017，3，6： | 3 |
| 18 | Tranexamic acid for acute intracerebral haemorrhage growth based on imaging assessment (TRAIGE): a multicentre, randomised, placebo- controlled trial显示文摘Background Studies show tranexamic acid can reduce the risk of death and early neurological deterioration after intracranial haemorrhage.We aimed to assess whether tranexamic acid reduces haematoma expansion and improves outcome in intracerebral haemorrhage patients susceptible to haemorrhage expansion.Methods We did a prospective,double-blind,randomised,placebo-controlled trial at 10 stroke centres in China.Acute supratentorial intracerebral haemorrhage patients were eligible if they had indication of haemorrhage expansion on admission imaging(eg,spot sign,black hole sign or blend sign),and were treatable within 8 hours of symptom onset.Patients were randomly assigned(1:1)to receive either tranexamic acid or a matching placebo.The primary outcome was intracerebral haematoma growth(>33% relative or>6 mL absolute)at 24 hours.Clinical outcomes were assessed at 90 days.Results Of the 171 included patients,124(72.5%)were male,and the mean age was 55.9±11.6 years.89 patients received tranexamic acid and 82 received placebo.The primary outcome did not differ significantly between the groups:36(40.4%)patients in the tranexamic acid group and 34(41.5%)patients in the placebo group had intracranial haemorrhage growth(OR 0.96,95% CI 0.52 to 1.77,p=0.89).The proportion of death was lower in the tranexamic acid treatment group than placebo group(8.1%vs 10.0%),but there were no significant differences in secondary outcomes including absolute intracranial haemorrhage growth,death and dependency.Conclusions Among patients susceptible to haemorrhage expansion treated within 8 hours of stroke onset,tranexamic acid did not significantly prevent intracerebral haemorrhage growth.Larger studies are needed to assess safety and efficacy of tranexamic acid in intracerebral haemorrhage patients. | Jingyi Liu Ximing Nie Hongqiu Gu Qi Zhou Haixin Sun Ying Tan Dacheng Liu Lina Zheng Jiahui Zhao Yan Wang Yibin Cao Haomeng Zhu Yunpeng Zhang Lijin Yi Yuehua Pu Miao Wen Zhonghua Yang Shengjun Sun Wenzhi Wang Xingquan Zhao Liping Liu Yongjun Wang | 2021 | Stroke & Vascular Neurology2021，6，2： | 3 |
| 19 | Dosimetric parameters of palladium-103 brachytherapy source with Monte Carlo simulation显示文摘Before clinical application of a new source, the dosimetric parameters of the source should be accu- rately determined. This work is dedicated to the Monte Carlo method to calculate dosimetric parameters as recommended by the American Association of Physicists in Medicine (AAPM) TG-43 guidelines for model ADVANTAGETM palladium-103 source and, through comparison with data from another published report for the same source, presents a suggested dataset for clinical applications. From these calcula- tions, tables are presented for the radial dose function and the anisotropy function of palladium-103 brachytherapy source. The dose rate constants are found to be 0.671 (cGyh-1U-1) in liquid water and 0.673 (cGyh-1U-1) in Solid WaterTM. And the anisotropy constants in liquid water and Solid WaterTM are found to be 0.864 and 0.865 respectively. Comparison with the previous study shows that our results of dosimetric parameters are in good agreement with those measured and calculated by Meigooni et al. (2006) both in Water and Solid WaterTM. | WANG JianHua LIU Wei XU XunJiang GU JiaHui CAI Jun HUA ZhengDong XU JiaQiang | 2008 | Chinese Science Bulletin2008，53，9： | 3 |
| 20 | A New Time-Aware Collaborative Filtering Intelligent Recommendation System显示文摘Aiming at the problem that the traditional collaborative filtering recommendation algorithm does not fully consider the influence of correlation between projects on recommendation accuracy,this paper introduces project attribute fuzzy matrix,measures the project relevance through fuzzy clustering method,and classifies all project attributes.Then,the weight of the project relevance is introduced in the user similarity calculation,so that the nearest neighbor search is more accurate.In the prediction scoring section,considering the change of user interest with time,it is proposed to use the time weighting function to improve the influence of the time effect of the evaluation,so that the newer evaluation information in the system has a relatively large weight.The experimental results show that the improved algorithm improves the recommendation accuracy and improves the recommendation quality. | Weijin Jiang Jiahui Chen Yirong Jiang Yuhui Xu Yang Wang Lina Tan Guo Liang | 2019 | Computers, Materials & Continua2019，，8： | 3 |
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